公开(公告)号：IT1224254B

公开(公告)日：1990-09-26

申请号：IT2013388

申请日：1988-04-08

Applicant: SCLAVO SPA

Inventor： GROSS ROY ,  RAPPUOLI RINO

IPC: C12N15/09 ,  C12N1/21 ,  C12N15/74 ,  C12P21/00 ,  C12R1/01 ,  C12R1/19 ,  C12N

公开(公告)号：IT8920554D0

公开(公告)日：1989-05-19

申请号：IT2055489

申请日：1989-05-19

Applicant: SCLAVO SPA

Inventor： MAGISTRIS MARIA TERESA DE ,  BARTOLONI ANTONELLA ,  RAPPUOLI RINO ,  TAGLIABUE ALDO

IPC: A61K38/00 ,  A61K39/10 ,  A61K39/39 ,  A61P37/00 ,  C07K7/06 ,  C07K7/08 ,  C07K14/235 ,  C07K19/00

公开(公告)号：IT1202612B

公开(公告)日：1989-02-09

申请号：IT1955187

申请日：1987-03-03

Applicant: ENIRICERCHE SPA ,  SCLAVO SPA

Inventor： BELLIN ADA VELATI ,  TOMA SALVATORE ,  GRANDI GUIDO ,  COLLETTI ELISABETTA ,  RIBOLI BARBARA ,  COSMINA PAOLA ,  TOGNONI ANGELO ,  PEDRONI PAOLA ,  RAPPUOLI RINO

IPC: A61K39/10 ,  A61K39/00 ,  A61K39/07 ,  C07K14/235 ,  C12N1/20 ,  C12N1/21 ,  C12N15/00 ,  C12N15/09 ,  C12N15/70 ,  C12N15/75 ,  C12P21/00 ,  C12P21/02 ,  C12Q1/04 ,  C12R1/07 ,  C12R1/125 ,  C12R1/19 ,  G01N33/569 ,  G01N33/577 ,  C12K

公开(公告)号：IT1167672B

公开(公告)日：1987-05-13

申请号：IT2402083

申请日：1983-12-05

Applicant: SCLAVO SPA

Inventor： RAPPUOLI RINO

IPC: C12N20060101 ,  C12N

Abstract: Method comprises cultivation of a microorganism of the Corynebacterium diphtheriae family having two mutant phages (bacterophages) carrying coding for the protein corresp. to the diphtheria toxin and integrated in non-tandem mode into their chromosomes. The cultivation is effected in a neutral liq. nutritive medium contg. 0.05-0.5 pref. 0.1, micrograms/ml of iron ions, at 30-40 pref. 37-37 deg.C, under aerobic conditions.

公开(公告)号：FR2540516A1

公开(公告)日：1984-08-10

申请号：FR8401863

申请日：1984-02-07

Applicant: SCLAVO SPA

Inventor： RAPPUOLI RINO

IPC: C12N15/09 ,  A61K39/05 ,  C07K14/34 ,  C12N15/00 ,  C12N15/12 ,  C12P21/00 ,  C12R1/16 ,  C12P21/02

Abstract: Process for the production of a protein correlated with the diphtheric toxin which comprises culturing in a liquid nutrient medium having a concentration of iron ions of from 0.05 mu g/ml to 0.5 mu g/ml, at a temperature of from 30 DEG C. to 40 DEG C., in a neutral culturing environment and under aerobic conditions, a microorganism belonging to the Corynebacterium diphtheriae genus with two mutant phages encoding the protein correlated with the diphtheric toxin integrated in a nontandem mode in their chromosomes.

公开(公告)号：IT8324020D0

公开(公告)日：1983-12-05

申请号：IT2402083

申请日：1983-12-05

Applicant: SCLAVO SPA

Inventor： RAPPUOLI RINO

IPC: C12N20060101 ,  C12N

公开(公告)号：CA2015677C

公开(公告)日：2005-06-21

申请号：CA2015677

申请日：1990-04-27

Applicant: SCLAVO SPA

Inventor： PIZZA MARIAGRAZIA ,  COVACCI ANTONELLO ,  RAPPUOLI RINO ,  NENCIONI LUCIANO

IPC: C12N1/20 ,  A61K39/00 ,  A61K39/10 ,  C07K14/235 ,  C12N15/00 ,  C12N15/01 ,  C12N15/09 ,  C12N15/31 ,  C12R1/01 ,  C12N1/21 ,  C12N9/10

Abstract: New pertussis toxin (PT) mutants are described being immunologically active and having reduced or no toxicity, characterized in that at least one of the aminoacid residues G1u129, Asp11, Trp26, Arg9, Phe50, Asp1, Arg13, Tyr130, Gly86, Ile88, Tyr89, Tyr8, Gly 44, Thr53 and G1y80 of subunit's 1 aminoacid sequence is deleted and substituted by a different aminoacid residue selected in the group of natural aminoacids; Bordetella strains capable of providing and secreting said PT mutants and means and methods for obtaining them are also described. The Bordetella strains and the PT mutants produced by them are particularly suitable for the preparation of effective cellular and acellular antipertussis vaccines.

公开(公告)号：CA1341123C

公开(公告)日：2000-10-17

申请号：CA614474

申请日：1989-09-29

Applicant: SCLAVO SPA ,  UNIV LELAND STANFORD JUNIOR

Inventor： FALKOW STANLEY ,  RELMAN DAVID A ,  RAPPUOLI RINO ,  DOMENIGHINI MARIO

IPC: C12N15/09 ,  A61K31/70 ,  A61K38/00 ,  A61K39/00 ,  A61K39/10 ,  A61K39/395 ,  A61P37/04 ,  C07K14/235 ,  C12N1/21 ,  C12N5/10 ,  C12N9/12 ,  C12P21/02 ,  C12R1/01 ,  C12R1/19 ,  C12N15/31 ,  C12N1/20 ,  A61P31/00

Abstract: Nucleic acid and protein compositions are provided from B. pertussis which may find use in diagnosis, prevention and therapy of whooping cough. Particularly, an open reading frame encoding filamentous hemagglutinin precursors provided, with the intact protein for the filamentous hemagglutinin portion thereof, can be expressed in a wide variety of hosts, for use in the production of antibodies, for immunodiagnosis or therapy, or as vaccines for prophylactic purposes.

公开(公告)号：HK17096A

公开(公告)日：1996-02-09

申请号：HK17096

申请日：1996-02-01

Applicant: SCLAVO SPA

Inventor： PIZZA MARIGRAZIA ,  COVACCI ANTONELLO ,  RAPPUOLI RINO ,  NENCIONI LUCIANO

IPC: C12N1/20 ,  A61K39/00 ,  A61K39/10 ,  C07K14/235 ,  C12N15/00 ,  C12N15/01 ,  C12N15/09 ,  C12N15/31 ,  C12R1/01

公开(公告)号：HK192995A

公开(公告)日：1995-12-29

申请号：HK192995

申请日：1995-12-21

Applicant: SCLAVO SPA

Inventor： PIZZA MARIAGRAZIA ,  RAPPUOLI RINO ,  BARTOLONI ANTONELLA

IPC: A61K39/00 ,  A61K39/10 ,  A61P31/04 ,  C07H21/04 ,  C07K14/00 ,  C07K14/195 ,  C07K14/235 ,  C07K14/41 ,  C12N1/20 ,  C12N1/21 ,  C12N15/09 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19 ,  C12N15/31

Abstract: Immunologically active polypeptides with no or reduced toxicity useful for the preparation of an antipertussis vaccine. Method for the preparation of said polypeptides which comprises, cultivating a microorganismtransformed with a hybrid plasmid including the gene/s which codes for at least one of said polypeptides in a suitable medium and recovering the desired polypeptide from the cells or from the culture medium.