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    DYNAMIC ARRAY ASSAY METHODS
    51.
    发明申请
    DYNAMIC ARRAY ASSAY METHODS 审中-公开
    动态阵列测定方法

    公开(公告)号：WO2009100449A1

    公开(公告)日：2009-08-13

    申请号：PCT/US2009/033586

    申请日：2009-02-09

    Applicant: FLUIDIGM CORPORATION ,  LIVAK, Kenneth J. ,  UNGER, Marc

    Inventor： LIVAK, Kenneth J. ,  UNGER, Marc

    IPC: C12M1/34

    CPC classification number: C12Q1/686 ,  C12Q1/6834 ,  C12Q2565/629

    Abstract: High throughput methods are used that combine the features of using a matrix-type microfluidic device, labeled nucleic acid probes, and homogenous assays to detect and/or quantify nucleic acid analytes. The high throughput methods are capable of detecting nucleic acid analyes with high PCR and probe specificity, producing a low fluorescence background and therefore, a high signal to noise ratio. Additionally, the high throughput methods are capable of detecting low copy number nucleic acid analyte per cell.

    Abstract translation: 使用高通量方法，其结合使用基质型微流体装置，标记的核酸探针和均质测定的特征来检测和/或定量核酸分析物。 高通量方法能够以高PCR和探针特异性检测核酸分析，产生低荧光背景，因此具有高的信噪比。 此外，高通量方法能够检测每个细胞的低拷贝数核酸分析物。

    METHOD AND SYSTEM FOR CRYSTALLIZATION AND X-RAY DIFFRACTION SCREENING
    52.
    发明申请
    METHOD AND SYSTEM FOR CRYSTALLIZATION AND X-RAY DIFFRACTION SCREENING 审中-公开

    公开(公告)号：WO2008141183A3

    公开(公告)日：2008-11-20

    申请号：PCT/US2008/063247

    申请日：2008-05-09

    Applicant: FLUIDIGM CORPORATION ,  FOWLER, Brian ,  MAY, Andrew

    Inventor： FOWLER, Brian ,  MAY, Andrew

    IPC: H05G1/00

    Abstract: An integrated fluidic circuit includes a substrate layer and a first structure coupled to the substrate layer and including a plurality of channels. The first structure is configured to provide for flow of one or more materials through the plurality of channels. The integrated fluidic circuit also includes a second structure coupled to the substrate layer. The second structure includes a plurality of control channels configured to receive an actuation pressure. The integrated fluidic circuit is characterized by a thickness of less than 1.5 mm.

    SINGLE-CELL TRANSCRIPT SEQUENCING
    56.
    发明申请
    SINGLE-CELL TRANSCRIPT SEQUENCING 审中-公开

    公开(公告)号：WO2018013723A9

    公开(公告)日：2018-01-18

    申请号：PCT/US2017/041770

    申请日：2017-07-12

    Applicant: FLUIDIGM CORPORATION

    Inventor： LIVAK, Kenneth J. ,  FEKETE, Richard A.

    IPC: C12Q1/68

    Abstract: Described herein are methods for preparing DNA templates for single-cell transcript sequencing of RNA from a population of cells. The methods entail distributing cells from the population into separate reaction volumes so that a plurality of separate reaction volumes each contain a single, isolated cell, wherein the cells have been treated with a fixative prior to distribution. The isolated cells are then permeabilized or disrupted, and cDNA is prepared by reverse transcript, followed by amplification. Also provided is a novel chemistry for efficient production of DNA templates from T-cell receptors or immunoglobulins in single cells.

    HAPLOIDOME DETERMINATION BY DIGITIZED TRANSPOSONS
    57.
    发明申请
    HAPLOIDOME DETERMINATION BY DIGITIZED TRANSPOSONS 审中-公开
    通过DIGITIZED TRANSPOSONS进行快速测定

    公开(公告)号：WO2015179706A1

    公开(公告)日：2015-11-26

    申请号：PCT/US2015/032066

    申请日：2015-05-21

    Applicant: FLUIDIGM CORPORATION

    Inventor： XI, Lei ,  WANG, Xiaohui ,  UNGER, Marc ,  RUFF, David

    IPC: C12N15/10 ,  C12Q1/68

    CPC classification number: C12N15/1082 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q2525/155 ,  C12Q2525/301 ,  C12Q2535/122

    Abstract: In certain embodiments, the present invention provides a way of "digitally" marking different the alleles of different chromosomes by using a transposase to insert differently barcoded transposons into genomic DNA before further analysis. According to this method, each allele becomes marked with a unique pattern of transposon barcodes. Because each unique pattern of transposon barcodes identifies a particular allele, the method facilitates determinations of ploidy and copy number variation, improves the ability to discriminate among homozygotes, heterozygotes, and patterns arising from sequencing errors, and allows loci separated by uninformative stretches of DNA to be identified as linked loci, thereby facilitating haplotype determinations. Also provided is a novel artificial transposon end that includes a barcode sequence in two or more positions that are not essential for transposition.

    Abstract translation: 在某些实施方案中，本发明提供了一种通过使用转座酶在进一步分析之前将不同的条形码转座子插入基因组DNA来“不同”地标记不同染色体等位基因的方法。 根据这种方法，每个等位基因都被标记为转座子条形码的独特模式。 因为转座子条形码的每个独特模式识别特定的等位基因，所以该方法有助于确定倍性和拷贝数变异，提高了鉴别纯合子，杂合子和测序错误引起的模式的能力，并且允许通过DNA的非信息延伸分离的位点 被确定为相关基因座，从而促进单倍型测定。 还提供了一种新颖的人工转座子末端，其包括对于转座不是必需的两个或更多个位置的条形码序列。

    NUCLEIC ACID DETECTION USING PROBES
    59.
    发明申请
    NUCLEIC ACID DETECTION USING PROBES 审中-公开
    使用探针进行核酸检测

    公开(公告)号：WO2014077822A1

    公开(公告)日：2014-05-22

    申请号：PCT/US2012/065376

    申请日：2012-11-15

    Applicant: FLUIDIGM CORPORATION

    Inventor： LIVAK, Kenneth ,  MEYERS, Stacy ,  WANG, Xiaohui ,  WANG, Jun

    IPC: C12Q1/68

    CPC classification number: C12Q1/6827 ,  C12Q1/686 ,  C12Q2521/101 ,  C12Q2525/185 ,  C12Q2535/125 ,  C12Q2563/107 ,  C12Q2565/101 ,  C12Q2565/629

    Abstract: The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm2, comprising a sequence segment that complementary to the regulatory sequence and a tail segment that does not hybridize to the probe nucleotide when the sequence segment and the regulatory sequence are annealed, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and using a DNA polymerase with high strand displacement activity and low 5-nuclease activity, and detecting the amplification product; wherein Tm1 and Tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.

    Abstract translation: 本发明提供了通过用核苷酸标签标记核苷酸序列来检测靶核苷酸序列的方法，提供了具有调节序列和核苷酸标签识别序列的融解温度Tm1的探针寡核苷酸; 在多核苷酸扩增反应中将探针寡核苷酸掺入标记的多核苷酸，提供具有解链温度Tm2的调节性寡核苷酸，其包含与调节序列互补的序列区段和当序列片段不与探针核苷酸杂交时的尾段 使用探针寡核苷酸作为引物，并使用具有高链置换活性和低5-核酸酶活性的DNA聚合酶，并检测扩增产物，在PCR扩增反应中扩增标记的靶核酸序列，调节序列退火， 其中Tm1和Tm2高于与多核苷酸扩增反应相关的退火温度。

    MULTIFUNCTIONAL PROBE-PRIMERS
    60.
    发明申请
    MULTIFUNCTIONAL PROBE-PRIMERS 审中-公开
    多功能探测器

    公开(公告)号：WO2012106668A2

    公开(公告)日：2012-08-09

    申请号：PCT/US2012/023870

    申请日：2012-02-03

    Applicant: FLUIDIGM CORPORATION ,  LIVAK, Kenneth J.

    Inventor： LIVAK, Kenneth J.

    IPC: E21B47/06

    CPC classification number: C12Q1/6876 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q2525/161 ,  C12Q2525/197 ,  C12Q2565/1015

    Abstract: Methods and reagents for detection and analysis of nucleic acids are provided. Certain methods involves an encoding amplification in which a target sequence is associated with probe-binding sequences and optionally with indexing sequences, (2) an optional distribution step in which the product of the encoding amplification is split into multiple aliquots, and (3) a decoding and detection step in which the presence, absence, quantity, or relative amount of the target sequence in the aliquots is determined. The detection step makes use of a multifunctional "self-digesting" molecular probe comprising a primer polynucleotide and a probe oligonucleotide, linked in a 5'-5' orientation.

    Abstract translation: 提供了用于检测和分析核酸的方法和试剂。 某些方法涉及编码扩增，其中靶序列与探针结合序列和任选地与索引序列相关，（2）任选的分布步骤，其中编码扩增的产物被分成多个等分试样，和（3）a 解码和检测步骤，其中确定等分试样中靶序列的存在，不存在，数量或相对量。 检测步骤使用包含以5'-5'方向连接的引物多核苷酸和探针寡核苷酸的多功能“自消化”分子探针。

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