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公开(公告)号：KR1020010038030A
公开(公告)日：2001-05-15
申请号：KR1019990045835
申请日：1999-10-21
Applicant: 한국해양연구원
IPC: C12N1/20
Abstract: PURPOSE: A facultative anaerobic microorganism, Shewanella putrefaciens DK-1, and a method for decomposing oil pollutants using the same are provided. Since the facultative anaerobic microorganism is viable in the presence of oxygen, they can effectively decompose oil pollutants even if in the presence of air. CONSTITUTION: The facultative anaerobic microorganism, Shewanella putrefaciens DK-1 (KCTC 8966P), which is viable in the presence or absence of air, decomposes oil pollutants containing aliphatic compounds such as hexadecane and uses oxygen, NO3 and Fe(III) as a final electron receptor. The oil pollutants, which are produced by the leakage of oil from oil tank, wrong treatment of petroleum compounds, and so forth, are biologically decomposed by adding the fermented culture of Shewanella putrefaciens DK-1 (KCTC 8966P) into the oil pollutants.
Abstract translation: 目的：提供兼性厌氧微生物，腐败假丝酵母DK-1，以及使用该方法分解油污染物的方法。 由于兼性厌氧微生物在氧气存在下是可行的，所以即使在存在空气的情况下也能有效地分解油污染物。 构成：在存在或不存在空气的情况下存活的兼性厌氧微生物Shevanella putrefaciens DK-1（KCTC 8966P）分解含有脂肪族化合物如十六烷的油污染物，并使用氧，NO 3和Fe（III）作为最终 电子受体。 通过将油菜腐败菌DK-1（KCTC 8966P）的发酵培养物添加到油污染物中，通过油罐漏油，石油化合物处理错误等产生的油污染物生物分解。
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公开(公告)号：KR101203920B1
公开(公告)日：2012-11-22
申请号：KR1020080116102
申请日：2008-11-21
Applicant: 한국해양연구원
Abstract: 본 발명은
Thermococcus 속에 속하는 고호열성 신균주로부터 분리한 신규한 포스파타제(Phosphatase) 및 이를 암호화하는 유전자에 관한 것이다. 본 발명의 포스파타제는 고온에서도 열안정성을 갖기 때문에 특정 단백질이나 DNA 대상체를 검출하기 위한 마커 등으로 널리 이용될 수 있다.
포스파타제, HAD 슈퍼패밀리-
公开(公告)号：KR101059967B1
公开(公告)日：2011-08-29
申请号：KR1020080106307
申请日：2008-10-29
Applicant: 한국해양연구원
Abstract: 본 발명은 심해 퇴적물을 배양하여 얻어지며 혈관신생유도 억제 물질을 생산하는 스트렙토마이스세스(
Streptomyces ) 속 미생물 및 이를 이용한 항암제 조성물에 관한 것으로서, 심해 퇴적물을 60℃에서 50분 간 열처리하는 단계와 멸균 해수에 상기 열처리한 심해 퇴적물을 희석한 후 여과한 다음 멸균 해수에 재희석하여 배지에 배양하는 단계에 의해 혈관신생유도 억제 물질을 생산하는 스트렙토마이스세스(
Streptomyces ) 속 미생물을 분리하는 것으로서 분리된 스트렙토마이스세스(
Streptomyces ) 속 미생물은 기탁번호 KFCC11406P로 기탁되어 있다. 또한 본 발명은 기탁된 균주, 상기 균주의 배양물 및 상기 균주의 배양여과액으로 이루어진 군으로부터 선택되는 어느 하나 이상을 포함하여 혈관신생유도 억제를 통한 항암 활성을 갖는 것을 특징으로 하는 항암제 조성물을 제공한다.
심해 퇴적물, 혈관신생유도, 스트렙토마이세스Abstract translation: 本发明涉及通过培养深海沉积物而产生血管生成抑制剂的方法，
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Patent Agency Ranking
公开(公告)号：KR1020110069283A
公开(公告)日：2011-06-23
申请号：KR1020090125952
申请日：2009-12-17
Applicant: 한국해양연구원
Abstract: PURPOSE: A gene encoding a protein isolated from Thermococcus sp. NA1 is provided to be applied to textile, food, paper, and distillation industries and clinical and pharmaceutical fields. CONSTITUTION: A useful gene isolated from Thermococcus sp. NA1 is selected from sequence numbers 1-2031. A useful protein isolated from Thermococcus sp. NA1 is selected from sequence numbers 2032-4062. A recombinant vector contains the useful gene. A transformed cell is obtained by transforming host cells of prokaryote, fungi, plant cells, and animal cells using the recombinant vector. The host cell is E.coli. The useful protein is prepared by culturing the transformed cells.
Abstract translation: 目的：编码从Thermococcus sp。分离的蛋白质的基因。 NA1适用于纺织，食品，造纸，蒸馏工业以及临床和制药领域。 构成：从Thermococcus sp。分离的有用基因。 NA1从序列号1-2031中选择。 从Thermococcus sp。分离的有用蛋白质 NA1选自序列号2032-4062。 重组载体含有有用的基因。 通过使用重组载体转化原核生物，真菌，植物细胞和动物细胞的宿主细胞获得转化的细胞。 宿主细胞是大肠杆菌。 通过培养转化细胞制备有用的蛋白质。
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公开(公告)号：KR1020110046412A
公开(公告)日：2011-05-04
申请号：KR1020110025582
申请日：2011-03-22
Applicant: 한국해양연구원
Abstract: PURPOSE: A novel phosphatase isolated from Thermococcus strain and a gene encoding the same are provided to ensure thermal stability and to be used as a marker. CONSTITUTION: A novel phosphatase has an amino acid of sequence number 9. The novel phosphatase is produced from Thermococcus onnurineus NA1(deposit number: KCTC 10859BP) which generates hydrogen under anaerobic condition. A gene encoding the phosphatase contains a base sequence of sequence number 4. The novel phosphatase is produced by culturing Thermococcus onnurineus NA1(deposit number: KCTC 10859BP).
Abstract translation: 目的：提供从Thermococcus菌株分离的新型磷酸酶及其编码基因，以确保热稳定性并用作标记物。 构成：新型磷酸酶具有序列号9的氨基酸。新型磷酸酶由嗜热厌氧条件下产生氢的Thermococcus onnurineus NA1（保藏号：KCTC 10859BP）产生。 编码磷酸酶的基因含有序列号4的碱基序列。新型磷酸酶是通过培养嗜热热球菌NA1（保藏号：KCTC 10859BP）产生的。
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公开(公告)号：KR1020110046411A
公开(公告)日：2011-05-04
申请号：KR1020110025581
申请日：2011-03-22
Applicant: 한국해양연구원
Abstract: PURPOSE: A novel phosphatase isolated from Thermococcus onnurineus NA1 and agene encoding the same are provided to be used as a marker for detecting DNA object or certain protein. CONSTITUTION: A phosphatase isolated from Thermococcus onnurineus NA1 contains an amino acid of sequence number 8. The Thermococcus onnurineus NA1 has deposit number KCTC 10859BP. The novel strain is isolated from deep seawater in East Manus Basin. The novel phosphatase is produced by culturing Thermococcus onnurineus NA1(deposit number: KCTC 10859BP).
Abstract translation: 目的：提供从Thermococcus Onnurineus NA1分离的新型磷酸酶和编码该蛋白的Agene，用作检测DNA物质或某些蛋白质的标记物。 构成：从Thermococcus onnurineus NA1分离的磷酸酶含有序列号8的氨基酸。Thermococcus onnurineus NA1具有保藏号KCTC 10859BP。 新型菌株从东马努斯盆地的深海水中分离出来。 新型磷酸酶通过培养嗜热热球菌NA1（保藏号：KCTC 10859BP）产生。
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公开(公告)号：KR1020100047423A
公开(公告)日：2010-05-10
申请号：KR1020080106307
申请日：2008-10-29
Applicant: 한국해양연구원
Abstract: PURPOSE: A Streptomyces genus microorganism isolated from deep sea sediment is provided to produce a material which suppresses vascularization and use as an anti-cancer composition. CONSTITUTION: A Streptomyces genus microorganism producing a material which suppresses vascularization is obtained by culturing deep sea sediment. A method for isolating Streptomyces genus microorganism comprises: a step of heating the deep sea sediment at 60°C for 50 minutes; a step of diluting the sediment and filtering; and a step of re-diluting and culturing in a medium. The Streptomyces genus microorganism is deposited in the number of KFCC1406P. An anti-cancer composition having an anti-cancer activity through suppression of vascularization contains the Streptomyces genus microorganism of deposit number KFCC1406P, culture medium of the strain, or filtered liquid of the culture.
Abstract translation: 目的：提供从深海沉积物分离的链霉菌属微生物，以产生抑制血管形成和用作抗癌成分的物质。 构成：通过培养深海沉积物获得产生抑制血管形成的材料的链霉菌属微生物。 分离链霉菌属微生物的方法包括：在60℃加热深海沉积物50分钟的步骤; 稀释沉淀物和过滤的步骤; 以及在培养基中再稀释和培养的步骤。 链霉菌属微生物以KFCC1406P的数量沉积。 通过抑制血管形成具有抗癌活性的抗癌组合物含有存在编号KFCC1406P的链霉菌属微生物，菌株的培养基或培养物的过滤液体。
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公开(公告)号：KR100825279B1
公开(公告)日：2008-04-25
申请号：KR1020060119613
申请日：2006-11-30
Applicant: 한국해양연구원
IPC: C07K14/195
CPC classification number: C12N9/16
Abstract: A HAM-1 like protein is provided to increase the processivity of DNA polymerase when used with the DNA polymerase, thereby being used for all reactions requiring DNA polymerization and applied to DNA amplification requiring high sensitivity and rapidity. A protein is described as SEQ ID : NO. 1 and is characterized in that it increases DNA polymerization processivity. A nucleic acid sequence encodes the protein and is described as SEQ ID : NO. 2. The protein is used in order to increase DNA polymerization by DNA polymerase. A host cell transformed by a recombinant vector including a gene described as SEQ ID : NO. 2 is used in order to produce the protein. The DNA polymerase and the protein are used in order to increase DNA replication.
Abstract translation: 提供了一种类似HAM-1的蛋白质，以增加DNA聚合酶与DNA聚合酶的连续性，从而被用于需要DNA聚合的所有反应，并应用于需要高灵敏度和快速度的DNA扩增。 蛋白质描述为SEQ ID NO： 1，其特征在于其增加DNA聚合反应活性。 核酸序列编码该蛋白质并描述为SEQ ID NO： 2.蛋白质用于通过DNA聚合酶增加DNA聚合。 通过重组载体转化的宿主细胞，其包含如SEQ ID NO： 2用于产生蛋白质。 使用DNA聚合酶和蛋白质以增加DNA复制。
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