公开(公告)号：DE69223510D1

公开(公告)日：1998-01-22

申请号：DE69223510

申请日：1992-04-10

Applicant: ABBOTT LAB

Inventor： KLINE STEVEN ,  JOU YI-HER ,  STROUPE STEPHEN

IPC: G01N33/53 ,  G01N33/531 ,  G01N33/532 ,  G01N33/538 ,  G01N33/543 ,  G01N33/545 ,  G01N33/569 ,  G01N33/574 ,  G01N33/58 ,  G01N33/74 ,  G01N33/76 ,  G01N33/94 ,  G01N33/536 ,  G01N33/537 ,  G01N33/541 ,  G01N33/544 ,  G01N33/546 ,  G01N33/551 ,  G01N33/553

公开(公告)号：DE68924345T2

公开(公告)日：1996-04-25

申请号：DE68924345

申请日：1989-07-31

Applicant: ABBOTT LAB

Inventor： HILTIBRAN ROBERT G HILTIBRAN R ,  STROUPE STEPHEN DENHAM ,  JOU YI-HER

IPC: G01N33/532 ,  C07K14/435 ,  C07K14/76 ,  C08G69/10 ,  G01N33/538 ,  G01N33/542 ,  G01N33/543 ,  G01N33/574

Abstract: This invention presents novel polymeric anionic molecules and novel negatively charged capture reagents comprising the reaction products of said anionic molecules and a specific binding member for use in separation techniques and assay procedures wherein said activated polymeric anionic molecule comprises a compound having the formula: wherein n is about 10 to about 500; z is about 1 to about 6; W is selected from the group consisting of H , Na , K , Li , amine salts, and derivatives thereof; and X is a reactive group or a structure having a reactive group that enables the chemical binding of said activated polymer to a specific binding member.

公开(公告)号：DE69020094T2

公开(公告)日：1996-03-07

申请号：DE69020094

申请日：1990-08-27

Applicant: ABBOTT LAB

Inventor： KHALIL OMAR S ,  ZUREK THOMAS F ,  GENGER KEVIN R ,  PEPE CURTIS J ,  JOU YI-HER ,  COTTER STEPHEN M

IPC: G01N21/76 ,  G01N33/52 ,  G01N33/543 ,  G01N33/576 ,  G01N33/58

Abstract: Apparatus and method for performing a chemiluminescende assay involving the immobilization of a chemiluminescent reaction complex to a solid, porous element. The solid, porous element is preferably treated to provide an immobilizing interaction with the chemiluminescent reaction complex wherein the chemiluminescent reaction complex is thereby immobilized to the solid, porous element. The activating and reading of the chemiluminescent reaction are separately performed by evenly distributing a concentrated chemiluminescent activating solution to form a puddle on the surface of the porous element to which the chemiluminescent reaction complex is immobilized.

公开(公告)号：CA1309344C

公开(公告)日：1992-10-27

申请号：CA573112

申请日：1988-07-27

Applicant: ABBOTT LAB

Inventor： JOU YI-HER ,  HU ROGER C ,  LAGOCKI PETER A

IPC: G01N33/544 ,  G01N33/542 ,  G01N33/543 ,  G01N33/58 ,  G01N33/68 ,  G01N33/76

Abstract: "LIPOSOME BASED HOMOGENEOUS IMMUNOASSAY FOR DIAGNOSTIC TESTS" The present invention provides for novel homogeneous immunoassay systems involving complementmediated lysis of marker-encapsulating lipid vesicles (liposomes) for detection of analyte in a fluid sample. These systems do not require the separation of unbound antigens and/or antibody conjugates yet provide highly sensitive procedures for analyte detection. Liposomes containing a marker, are coupled to antibody fragments in a way which confers the liposomes with immunological specificity yet avoids sensitizing the liposomes to complement mediated lysis in the absence of analyte. Antibody sensitized liposomes (the first reagent) are sequentially incubated with an analytecontaining sample, and optionally "dummy" liposomes, which do not contain encapsulated marker, a second antibody (the second reagent), and finally with a complement source such as plasma. Complement is activated by the liposome-antibody-antigen-second antibody complex causing liposome lysis and a concomitant release of marker. Antibody of the first reagent may be an anti-analyte F(ab')2 antibody fragment, or an anti-analyte Fab' antibody fragment. Antibody of the second reagent may be provided in either soluble form, or in insoluble form e.g., bound onto carboxylated polystyrene particles or coupled to a third antibody in the form of a "double antibody" immune precipitate. Where the antibody of the second reagent is provided in an insoluble form, the analyte-containing sample is preferably incubated with the second reagent, to form an analyte-second antibody complex, prior to incubation with the first reagent. Where the second reagent consists of soluble antibodies, the first reagent is incubated with the analyte containing sample prior to incubation with the second reagent. Also provided are methods for preparing antibody sensitized liposomes in the presence of a polysaccharide capable of forming a reversible gel and methods for preparing derivatized Fab' antibody fragments for coupling to lipid vesicles.

公开(公告)号：AU9146491A

公开(公告)日：1992-08-17

申请号：AU9146491

申请日：1991-12-23

Applicant: ABBOTT LAB

Inventor： KHALIL OMAR S ,  GENGER KEVIN R ,  COTTER STEPHEN M ,  JOU YI-HER ,  ABUNIMEH NOMAN A ,  HILTIBRAND ROBERT G ,  STROUPE STEPHEN D

IPC: G01N33/532 ,  C12Q1/68 ,  G01N33/543 ,  G01N33/58 ,  C12Q1/00 ,  G01N33/53 ,  G01N33/566

Abstract: Assays for the determination of multiple analytes which may be present in a test sample by using different short-lived and long-lived chemiluminescent labels and integrating the generated chemiluminescence signal and time-discriminating the short-lived and the long-lived components of the signal generated. Also provided are assay kits which contain these short-lived and long-lived chemiluminescent compounds.