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公开(公告)号：WO9511083A3
公开(公告)日：1995-08-31
申请号：PCT/US9412125
申请日：1994-10-21
Applicant: ABBOTT LAB
Inventor： HANLEY KATHLEEN A , HOFFERBERT A DAVID , LEE HELEN H , PEPE CURTIS J , PERKO TIMOTHY J , ZUREK THOMAS F
CPC classification number: B01L3/50825 , B01L7/52
Abstract: A disposable reaction vessel for performing nucleic acid amplification assay. The disposable reaction vessel has a penetrable cap that can be penetrated by an automated pipettor to aspirate a portion of an amplified reaction product. The disposable reaction vessel contains the reagents necessary to perform a nucleic acid amplification assay. A patient speciment is added to the unit dose reagents in the disposable reaction vessel and the penetrable cap is closed. The disposable reaction vessel containing the reaction mixture and the specimen undergoes amplification, typically by placing it in a thermal cycler. After amplification the intact disposable reaction vessel is transferred to an automated analyzer where an automated pipettor penetrates the closure membrane and aspirates a portion of the amplified sample for further processing, without removal of the reaction vessel cap. This avoids the generation of potentially contaminating aerosols or droplets.
Abstract translation: 本发明涉及一种用于扩增和检测核酸材料的方法，包括以下步骤：将疑似含有靶核酸材料的样品与标记的试剂一起加入到扩增容器中，以将可疑的靶核酸扩增至反应 混合物; 通过关闭具有可由移液器探针穿透的膜的紧密密封盖将反应混合物密封在容器内; 扩增血管内的靶核酸物质; 从容器中取出一部分反应混合物进行检测; 并通过检测标记的试剂检测扩增的靶核酸的存在; 其中通过用移液器探针刺穿膜来实现去除，将反应混合物的一部分吸入移液器并将该部分分配在不同的检测室中，而不打开容器，从而避免可能污染的扩增材料的液滴或气溶胶 环境，未反应的样品或试剂。 用于进行核酸扩增测定的反应容器装置包括：热稳定聚合材料管，具有尺寸设计成适合于热循环装置的外径，该管具有到内部的开口; 用于紧密地密封管的开口的帽，帽包括不大于0.0381cm厚度的可刺穿的膜，由此膜允许用自动移液器从封闭管中对扩增的反应产物进行取样而不打开管; 以及将盖保持在管上并允许将帽折叠到开口中的柔性铰链。
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公开(公告)号：AT449645T
公开(公告)日：2009-12-15
申请号：AT02005296
申请日：1994-10-21
Applicant: ABBOTT LAB
Inventor： HANLEY A KATHLEEN , HOFFERBERT A DAVID , LEE HELEN H , PEPE CURTIS J , PERKO TIMOTHY J , ZUREK THOMAS F
Abstract: The present invention relates to a method for amplifying and detecting nucleic acid materials comprising the steps of: adding a sample suspected to contain a target nucleic acid material to an amplification vessel along with labeled reagents for amplification of the suspected target nucleic acid to from a reaction mixture; sealing the reaction mixture inside the vessel by closing a tightly sealing cap having a membrane that is penetrable by a pipettor probe; amplifying the target nucleic acid material within the vessel; removing a portion of the reaction mixture from the vessel for detection; and detecting the presence of amplified target nucleic acid by detection of the labeled reagents; wherein the removing is effected by piercing the membrane with the pipettor probe, aspirating the portion of the reaction mixture into the pipettor and dispensing the portion in a distinct detection compartment without uncapping the vessel, thereby avoiding drops or aerosols of the amplified material which might contaminate the environment, unreacted samples or reagents. The reaction vessel device for performing the nucleic acid amplification assay comprises:a tube of thermally stable polymeric material having an outer diameter dimensioned to fit into a thermal cycling apparatus, the tube having an opening to an interior; a cap for tightly sealing the opening of the tube, the cap including a puncturable membrane of not more than 0.0381 cm thickness, whereby the membrane allows sampling the amplified reaction product from the closed tube with an automated pipettor without opening the tube; and a flexible hinge that holds the cap to the tube and permits folding of the cap into the opening.
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公开(公告)号：CA2346951A1
公开(公告)日：2000-04-20
申请号：CA2346951
申请日：1999-10-12
Applicant: ABBOTT LAB
Inventor： DAHLKE DANIEL G , HALAKA FOLIM , TOTH JULIUS , SAFAR SCOTT G , KUKLA RONALD E , CLEMENS JOHN M , ZUCK GARY , BACH MARK C , GALITZ CHARLES M , CARTER MICHAEL K , BAY DANIEL , PIERCE MARK , PEPE CURTIS J , GRAY ROBERT C , HERCHENBACH STEVE
Abstract: A method of performing a determination of an item of interest in a sample using a single structure is disclosed. A sample is provided accesssible to t he single structure. A first container for processing the sample is placed in a first process path on the single structure. The sample is transferred to the first container in the first process path. A reagent is added to the first container in the first process path. Contents of the first container is mixe d in the first process path. The item of interest in the sample is separated from the contents of the first container in the first process path. The separated item of interest in the sample is transferred from the first container in the first process path to a second container in a second proces s path on the single structure. Contents of the second container are brought t o a first temperature different from a temperature of the first process path i n the second process path. The item of interest in the second container is detected in the second process path.
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公开(公告)号：ES2334890T3
公开(公告)日：2010-03-17
申请号：ES02005296
申请日：1994-10-21
Applicant: ABBOTT LAB
Inventor： HANLEY A KATHLEEN , HOFFERBERT A DAVID , LEE HELEN H , PEPE CURTIS J , PERKO TIMOTHY J , ZUREK THOMAS F
Abstract: Un método para amplificar y detectar materiales de ácido nucleico, que comprende las etapas de: a. añadir una muestra sospechosa de contener un material de ácido nucleico objetivo a un recipiente de amplificación (10) junto con reactivos marcados para amplificación de dicho ácido nucleico objetivo sospechoso para formar una mezcla de reacción; b. sellar la mezcla de reacción dentro de dicho recipiente, como se muestra con preferencia en las figuras 2 y 3 (30), cerrando una tapa herméticamente sellada, como se muestra con preferencia en las figuras 2 y 3, (20), que tiene una membrana, como se muestra con preferencia en las figuras 2 y 3, (28), que es penetrable por una sonda de pipeta, donde el recipiente de amplificación, como se muestra con preferencia en las figuras 2 y 3 (10), tiene una bisagra replegable, como se muestra con preferencia en las figuras 2 y 3, (30); c. amplificar el material de ácido nucleico objetivo dentro de dicho recipiente (10); d. retirar una porción de la mezcla de reacción desde el recipiente de amplificación (10) para detección; y e. detectar la presencia de ácido nucleico objetivo amplificado por detección de dichos reactivos marcados; en el que la retirada se realiza perforando la membrana (28) de la tapa con una sonda de pipeta, aspirando la porción de la mezcla de reacción en la pipeta y dispersando la porción en un compartimiento de detección distinto sin destapar el recipiente de amplificación (10), evitando de esta manera que gotas o aerosoles del material amplificado puedan contaminar el medio ambiente, muestras no reaccionadas o reactivos.
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公开(公告)号：CA2173861C
公开(公告)日：2003-12-23
申请号：CA2173861
申请日：1994-10-21
Applicant: ABBOTT LAB
Inventor： HANLEY KATHLEEN A , HOFFERBERT A DAVID , LEE HELEN H , PEPE CURTIS J , PERKO TIMOTHY J , ZUREK THOMAS F
IPC: G01N33/566 , B01L3/00 , B01L3/14 , B01L7/00 , B01L99/00 , C12M1/00 , C12N15/09 , C12Q1/68 , C12P19/34
Abstract: A disposable reaction vessel for performing nu- cleic acid amplification assay. The disposable reac- tion vessel has a penetrable cap that can be pene- trated by an automated pipettor to aspirate a portion of an amplified reaction product. The disposable re- action vessel contains the reagents necessary to per- form a nucleic acid amplification assay. A patient speciment is added to the unit dose reagents in the disposable reaction vessel and the penetrable cap is closed. The disposable reaction vessel containing the reaction mixture and the specimen undergoes ampli- fication, typically by placing it in a thermal cycler. After amplification the intact disposable reaction ves- sel is transferred to an automated analyzer where an automated pipettor penetrates the closure membrane and aspirates a portion of the amplified sample for further processing, without removal of the reaction vessel cap. This avoids the generation of potentially contaminating aerosols or droplets.
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Patent Agency Ranking
公开(公告)号：ES2075861T3
公开(公告)日：1995-10-16
申请号：ES90116380
申请日：1990-08-27
Applicant: ABBOTT LAB
Inventor： KHALIL OMAR S , ZUREK THOMAS F , GENGER KEVIN R , PEPE CURTIS J , JOU YI-HER , COTTER STEPHEN M
IPC: G01N21/76 , G01N33/52 , G01N33/543 , G01N33/576 , G01N33/58
Abstract: Apparatus and method for performing a chemiluminescende assay involving the immobilization of a chemiluminescent reaction complex to a solid, porous element. The solid, porous element is preferably treated to provide an immobilizing interaction with the chemiluminescent reaction complex wherein the chemiluminescent reaction complex is thereby immobilized to the solid, porous element. The activating and reading of the chemiluminescent reaction are separately performed by evenly distributing a concentrated chemiluminescent activating solution to form a puddle on the surface of the porous element to which the chemiluminescent reaction complex is immobilized.
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公开(公告)号：DE69435255D1
公开(公告)日：2010-01-07
申请号：DE69435255
申请日：1994-10-21
Applicant: ABBOTT LAB
Inventor： HANLEY KATHLEEN A , HOFFERBERT DAVID A , LEE HELEN H , PEPE CURTIS J , PERKO TIMOTHY J , ZUREK THOMAS F
Abstract: The present invention relates to a method for amplifying and detecting nucleic acid materials comprising the steps of: adding a sample suspected to contain a target nucleic acid material to an amplification vessel along with labeled reagents for amplification of the suspected target nucleic acid to from a reaction mixture; sealing the reaction mixture inside the vessel by closing a tightly sealing cap having a membrane that is penetrable by a pipettor probe; amplifying the target nucleic acid material within the vessel; removing a portion of the reaction mixture from the vessel for detection; and detecting the presence of amplified target nucleic acid by detection of the labeled reagents; wherein the removing is effected by piercing the membrane with the pipettor probe, aspirating the portion of the reaction mixture into the pipettor and dispensing the portion in a distinct detection compartment without uncapping the vessel, thereby avoiding drops or aerosols of the amplified material which might contaminate the environment, unreacted samples or reagents. The reaction vessel device for performing the nucleic acid amplification assay comprises:a tube of thermally stable polymeric material having an outer diameter dimensioned to fit into a thermal cycling apparatus, the tube having an opening to an interior; a cap for tightly sealing the opening of the tube, the cap including a puncturable membrane of not more than 0.0381 cm thickness, whereby the membrane allows sampling the amplified reaction product from the closed tube with an automated pipettor without opening the tube; and a flexible hinge that holds the cap to the tube and permits folding of the cap into the opening.
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公开(公告)号：AT169521T
公开(公告)日：1998-08-15
申请号：AT95906818
申请日：1995-01-11
Applicant: ABBOTT LAB
Inventor： ZUREK THOMAS F , HANLEY KATHLEEN A , PEPE CURTIS J
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公开(公告)号：AT123878T
公开(公告)日：1995-06-15
申请号：AT90116380
申请日：1990-08-27
Applicant: ABBOTT LAB
Inventor： KHALIL OMAR S , ZUREK THOMAS F , GENGER KEVIN R , PEPE CURTIS J , JOU YI-HER , COTTER STEPHEN M
IPC: G01N21/76 , G01N33/52 , G01N33/543 , G01N33/576 , G01N33/58
Abstract: Apparatus and method for performing a chemiluminescende assay involving the immobilization of a chemiluminescent reaction complex to a solid, porous element. The solid, porous element is preferably treated to provide an immobilizing interaction with the chemiluminescent reaction complex wherein the chemiluminescent reaction complex is thereby immobilized to the solid, porous element. The activating and reading of the chemiluminescent reaction are separately performed by evenly distributing a concentrated chemiluminescent activating solution to form a puddle on the surface of the porous element to which the chemiluminescent reaction complex is immobilized.
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公开(公告)号：CA2021586A1
公开(公告)日：1991-04-24
申请号：CA2021586
申请日：1990-07-19
Applicant: ABBOTT LAB
Inventor： KHALIL OMAR S , ZUREK THOMAS F , GENGER KEVIN R , PEPE CURTIS J , JOU YI-HER , COTTER STEPHEN M
IPC: G01N21/76 , G01N33/52 , G01N33/543 , G01N33/576 , G01N33/53 , G01N33/58
Abstract: Apparatus and method for performing a chemiluminescence assay involving the immobilization of a chemiluminescent reaction complex to a solid, porous element. The solid, porous element is preferably treated to provide an immobilizing interaction with the chemiluminescent reaction complex wherein the chemiluminescent reaction complex is thereby immobilized to the solid, porous element. The activating and reading of the chemiluminescent reaction are separately performed by evenly distributing a concentrated chemiluminescent activating solution to form a puddle on the surface of the porous element to which the chemiluminescent reaction complex is immobilized.
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