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    Chemiluminescent electron-rich aryl-substituted 1,2-dioxetane
    1.
    发明专利
    Chemiluminescent electron-rich aryl-substituted 1,2-dioxetane 审中-公开
    亮氨酸电子稀释的芳基取代的1,2-二氧杂环己烷

    公开(公告)号：JP2008164600A

    公开(公告)日：2008-07-17

    申请号：JP2007322868

    申请日：2007-12-14

    Applicant: Abbott Lab ,  アボット・ラボラトリーズAbbott Laboratories

    Inventor： WANG NAI-YI ,  HU ROGER C

    IPC: G01N33/533 ,  C07D321/00 ,  C07D493/04 ,  C07F7/18 ,  C07F9/09 ,  C07F9/655 ,  C07F9/6561 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/68 ,  C12Q1/70 ,  G01N21/76 ,  G01N21/78 ,  G01N33/543 ,  G01N33/58

    CPC classification number: G01N33/582 ,  C07F7/1856 ,  C07F9/65512 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q2334/00 ,  G01N33/533 ,  Y10S435/968

    Abstract: PROBLEM TO BE SOLVED: To provide a chemiluminescent electron-rich aryl-substituted 1,2-dioxetane compound. SOLUTION: In the compound, an aryl group is multiply substituted with suitable electron-donating groups such that a light-emitting pattern of a molecule results in a very high luminescent count, thus providing for a sensitive and precise assay for haptens, analytes, polynucleotides and the like. The substituted aryl-containing 1,2-dioxetane compound can be used as a direct label in an immunoassay or when derivatized with an appropriate leaving group, can be used as a substrate for an enzyme immunoassay. The unusual chemiluminescence of the compound allows the timing of the luminescent reaction to be exactly controlled. COPYRIGHT: (C)2008,JPO&INPIT

    Abstract translation: 待解决的问题：提供化学发光富电子的芳基取代的1,2-二氧杂环丁烷化合物。 解决方案：在化合物中，芳基被合适的给电子基团多取代，使得分子的发光图案导致非常高的发光计数，从而为半抗原提供灵敏和精确的测定， 分析物，多核苷酸等。 取代的含芳基的1,2-二氧杂环丁烷化合物可以用作免疫测定中的直接标记，或者当用合适的离去基团衍生化时，可以用作酶免疫测定的底物。 该化合物的不寻常的化学发光允许精确控制发光反应的时间。 版权所有（C）2008，JPO＆INPIT

    LIPOSOME BASED HOMOGENEOUS IMMUNOASSAY FOR DIAGNOSTIC TESTS
    4.
    发明专利
    LIPOSOME BASED HOMOGENEOUS IMMUNOASSAY FOR DIAGNOSTIC TESTS 未知

    公开(公告)号：AU620554B2

    公开(公告)日：1992-02-20

    申请号：AU2006988

    申请日：1988-07-27

    Applicant: ABBOTT LAB

    Inventor： JOU YI-HER ,  HU ROGER C ,  LAGOCKI PETER A

    IPC: G01N33/544 ,  G01N33/542 ,  G01N33/543 ,  G01N33/58 ,  G01N33/68 ,  G01N33/76 ,  C07K15/12 ,  G01N33/53 ,  G01N33/563 ,  G01N33/92

    Abstract: The present invention provides for novel homogeneous immunoassay systems involving complement-mediated lysis of marker-encapsulating lipid vesicles (liposomes) for detection of analyte in a fluid sample. Antibody sensitized liposomes (the first reagent) are sequentially incubated with an analyte-containing sample, and optionally "dummy" liposomes, which do not contain encapsulated marker, a second antibody (the second reagent), and finally with a complement source such as plasma. Complement is activated by the liposome-antibody-antigen-second antibody complex causing liposome lysis and a concomitant release of marker. Antibody of the first reagent may be an anti-analyte F(ab min )2 antibody fragment, or an anti-analyte Fab min antibody fragment. Antibody of the second reagent may be provided in either soluble form, or in insoluble form e.g., bound onto carboxylated polystyrene particles or coupled to a third antibody in the form of a "double antibody" immune precipitate. Also provided are methods for preparing antibody sensitized liposomes in the presence of a polysaccharide capable of forming a reversible gel and methods for preparing derivatized Fab min antibody fragments for coupling to lipid vesicles.

    LIPOSOME BASED HOMOGENEOUS IMMUNOASSAY FOR DIAGNOSTIC TESTS
    7.
    发明专利
    LIPOSOME BASED HOMOGENEOUS IMMUNOASSAY FOR DIAGNOSTIC TESTS 未知

    公开(公告)号：CA1309344C

    公开(公告)日：1992-10-27

    申请号：CA573112

    申请日：1988-07-27

    Applicant: ABBOTT LAB

    Inventor： JOU YI-HER ,  HU ROGER C ,  LAGOCKI PETER A

    IPC: G01N33/544 ,  G01N33/542 ,  G01N33/543 ,  G01N33/58 ,  G01N33/68 ,  G01N33/76

    Abstract: "LIPOSOME BASED HOMOGENEOUS IMMUNOASSAY FOR DIAGNOSTIC TESTS" The present invention provides for novel homogeneous immunoassay systems involving complementmediated lysis of marker-encapsulating lipid vesicles (liposomes) for detection of analyte in a fluid sample. These systems do not require the separation of unbound antigens and/or antibody conjugates yet provide highly sensitive procedures for analyte detection. Liposomes containing a marker, are coupled to antibody fragments in a way which confers the liposomes with immunological specificity yet avoids sensitizing the liposomes to complement mediated lysis in the absence of analyte. Antibody sensitized liposomes (the first reagent) are sequentially incubated with an analytecontaining sample, and optionally "dummy" liposomes, which do not contain encapsulated marker, a second antibody (the second reagent), and finally with a complement source such as plasma. Complement is activated by the liposome-antibody-antigen-second antibody complex causing liposome lysis and a concomitant release of marker. Antibody of the first reagent may be an anti-analyte F(ab')2 antibody fragment, or an anti-analyte Fab' antibody fragment. Antibody of the second reagent may be provided in either soluble form, or in insoluble form e.g., bound onto carboxylated polystyrene particles or coupled to a third antibody in the form of a "double antibody" immune precipitate. Where the antibody of the second reagent is provided in an insoluble form, the analyte-containing sample is preferably incubated with the second reagent, to form an analyte-second antibody complex, prior to incubation with the first reagent. Where the second reagent consists of soluble antibodies, the first reagent is incubated with the analyte containing sample prior to incubation with the second reagent. Also provided are methods for preparing antibody sensitized liposomes in the presence of a polysaccharide capable of forming a reversible gel and methods for preparing derivatized Fab' antibody fragments for coupling to lipid vesicles.

    8.
    发明专利
    未知

    公开(公告)号：ES2171423T3

    公开(公告)日：2002-09-16

    申请号：ES93925057

    申请日：1993-10-26

    Applicant: ABBOTT LAB

    Inventor： WANG NAI-YI ,  HU ROGER C

    IPC: C07D321/00 ,  C07D493/04 ,  C07F7/18 ,  C07F9/655 ,  C07F9/6561 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/68 ,  C12Q1/70 ,  G01N33/533 ,  G01N33/58 ,  C09K3/00 ,  C12Q1/00 ,  C07D327/02 ,  C07D305/00 ,  C07F9/06

    Abstract: Chemiluminescent electron-rich aryl-substituted 1,2-dioxetane compounds are disclosed in which the aryl group is poly-substituted with suitable electron-donating groups such that the light-emitting pattern of the molecule results in a very high luminescent count, thus providing for a sensitive and precise assay for haptens, analytes, polynucleotides and the like. These substituted aryl-containing 1,2-dioxetane compounds can be used as direct labels in an immunoassay or when derivatized with an appropriate leaving group, can be used as a substrate for a enzyme immunoassay. The unusual chemiluminescence of the compounds allows the timing of the luminescent reaction to be exactly controlled.

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