公开(公告)号：US20070133077A1

公开(公告)日：2007-06-14

申请号：US11339044

申请日：2006-01-25

Applicant: Steven Rogers ,  Nissim Elmaliach ,  Emanuel Elyasef ,  Alon Litman ,  Ron Naftali ,  Doron Meshulach

Inventor： Steven Rogers ,  Nissim Elmaliach ,  Emanuel Elyasef ,  Alon Litman ,  Ron Naftali ,  Doron Meshulach

IPC: G02B26/08

CPC classification number: G01N21/8806 ,  G01N21/9501 ,  G01N21/956 ,  G01N2201/10 ,  G01N2201/1042 ,  G01N2201/106

Abstract: A method for scanning a surface, consisting of focusing an array of optical beams using optics having an axis, so as to illuminate a region of the surface intercepted by the axis, such that each optical beam illuminates a portion of a respective sub-region within the region. The method further includes moving at least one of the array and the surface so as to cause a translation of the surface relative to the axis in a first direction. During the translation in the first direction, each of the optical beams is scanned over the respective sub-region in a second direction, which is different from the first direction.

Abstract translation: 一种用于扫描表面的方法，其包括使用具有轴的光学器件聚焦光束阵列，以便照亮由所述轴线截取的表面的区域，使得每个光束照亮所述光束中的相应子区域的一部分 该区域。 所述方法还包括移动所述阵列和所述表面中的至少一个，以使所述表面相对于所述轴线在第一方向上平移。 在第一方向的平移期间，每个光束在与第一方向不同的第二方向上在相应的子区域上被扫描。

公开(公告)号：US20070132831A1

公开(公告)日：2007-06-14

申请号：US11302695

申请日：2005-12-13

Applicant: Daniel Chu

Inventor： Daniel Chu

IPC: B41J15/14

CPC classification number: G01N21/253 ,  G01N2201/0446 ,  G01N2201/1042

Abstract: Scanning of a microarray is performed through a mask that exposes a plurality, but not all, of the sites of the microarray, and either the mask is movable relative to the microarray or the microarray is movable relative to the mask, or both. The mask is useful as a means of restricting the illumination of sites on the microarray to those that can be illuminated while the scan head is traveling at a steady, target velocity, blocking the passage of light between the scan head and the microarray at those points in the scan head trajectory where the scan head is either accelerating or decelerating. The mask is also useful for reducing background noise in the microarray image by preventing light spillage to sites adjacent to those being scanned.

Abstract translation: 微阵列的扫描通过暴露微阵列的多个但不是全部的掩模进行，并且掩模相对于微阵列是可移动的，或者微阵列相对于掩模或两者都是可移动的。 该掩模可用作将扫描头以稳定的目标速度行进的情况下将微阵列上的部位的照明限制在可照亮的位置的手段，阻止在那些点处扫描头和微阵列之间的光通过 在扫描头轨迹中扫描头正在加速或减速。 该掩模也可用于通过防止光泄漏到与扫描的那些相邻的位置来减少微阵列图像中的背景噪声。

公开(公告)号：US20060007439A1

公开(公告)日：2006-01-12

申请号：US11175955

申请日：2005-07-06

Applicant: Timothy Corcoran

Inventor： Timothy Corcoran

IPC: G01J3/30

CPC classification number: G01N21/6456 ,  G01J3/02 ,  G01J3/021 ,  G01J3/14 ,  G01J3/4406 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6486 ,  G01N2021/6417 ,  G01N2021/6423 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2201/10 ,  G01N2201/1042

Abstract: Methods and devices are disclosed which apply an excitation-emission matrix (EEM) to a heterogeneous, two-dimensional sample, allowing a considerably larger number of emitting, e.g. fluorescent, labels to be used simultaneously. This may be accomplished by employing a spectroscopic method of excitation-emission matrices which allows discrimination of species with similar emission spectra, and also allows positive identification of energy transfer between emitting species. The methods and devices may employ a novel excitation-light scanning technique which allows imaging of the emission from the heterogeneous sample both in two spatial dimensions (length and width) and in two spectral dimensions (excitation and emission wavelength). This light scanning technique maximizes the throughput of excitation light, increasing the sensitivity and hence the reading speed of the instrument.

Abstract translation: 公开了将激发发射矩阵（EEM）施加到异质二维样品的方法和装置，允许相当大量的发射，例如， 荧光，标签同时使用。 这可以通过采用激发发射矩阵的光谱方法来实现，其允许区分具有相似发射光谱的物质，并且还允许阳性鉴定发射物质之间的能量转移。 所述方法和装置可以采用新的激发光扫描技术，其允许在两个空间维度（长度和宽度）和两个光谱尺寸（激发和发射波长）中成像来自异质样品的发射。 这种光扫描技术使激发光的吞吐量最大化，从而提高了仪器的灵敏度和读取速度。

公开(公告)号：US06835946B2

公开(公告)日：2004-12-28

申请号：US09996672

申请日：2001-11-30

Applicant: Nobuhiko Ogura

Inventor： Nobuhiko Ogura

IPC: G21N2164

CPC classification number: G01N21/6456 ,  G01N21/6458 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2201/1042

Abstract: An image reading apparatus is adapted for irradiating an image carrier including a labeling substance contained in two-dimensionally distributed spots with a stimulating ray and photoelectrically detecting light released from the labeling substance, thereby producing image data, and the image reading apparatus includes at least one stimulating ray source for emitting a stimulating ray, a lens for shaping the stimulating ray emitted from the at least one stimulating ray source into a line beam, a sensor for photoelectrically detecting light released from the labeling substance, and a controller for performing a stimulation and detection step of irradiating the image carrier including the labeling substance contained in the two-dimensionally distributed spots with the line beam of the stimulating ray to stimulate the labeling substance, stopping irradiation with the line beam of the stimulating ray and causing the sensor to photoelectrically detect light released from the labeling substance after the completion of irradiation with the line beam of the stimulating ray. According to the thus constituted image reading apparatus, it is possible to produce low noise image data rapidly and with a simple operation by irradiating an image carrier including two-dimensionally distributed spots of a labeling substance such as a fluorescent substance, a radioactive labeling substance or the like with a stimulating ray to excite the labeling substance and photoelectrically detecting light released from the labeling substance.

Abstract translation: 一种图像读取装置适用于用包含在二维分布斑点中的标记物质的图像载体用刺激光线照射和光电检测从标记物质释放的光，从而产生图像数据，图像读取装置包括至少一个 用于将从至少一个刺激射线源发射的刺激射线成形为线束的透镜，用于光电检测从标记物质释放的光的传感器，以及用于进行刺激的控制器，以及 将包含二维分布斑点中的标记物质的图像载体用刺激射线的线束照射的刺激标记物质的停止检测步骤，停止用刺激射线的线束照射，使传感器进行光电检测 光从标签上释放出来 在用激发光束的线束照射完成后。 根据这样构成的图像读取装置，可以通过照射包含荧光物质，放射性标记物质等标记物质的二维分布的斑点的图像载体，并且通过简单的操作，可以产生低噪声图像数据。 其具有刺激性射线以激发标记物质并光电检测从标记物质释放的光。

公开(公告)号：US20020066866A1

公开(公告)日：2002-06-06

申请号：US09996672

申请日：2001-11-30

Applicant: FUJI PHOTO FILM CO., LTD.

Inventor： Nobuhiko Ogura

IPC: G01N021/64

CPC classification number: G01N21/6456 ,  G01N21/6458 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2201/1042

Abstract: An image reading apparatus is adapted for irradiating an image carrier including a labeling substance contained in two-dimensionally distributed spots with a stimulating ray and photoelectrically detecting light released from the labeling substance, thereby producing image data, and the image reading apparatus includes at least one stimulating ray source for emitting a stimulating ray, a lens for shaping the stimulating ray emitted from the at least one stimulating ray source into a line beam, a sensor for photoelectrically detecting light released from the labeling substance, and a controller for performing a stimulation and detection step of irradiating the image carrier including the labeling substance contained in the two-dimensionally distributed spots with the line beam of the stimulating ray to stimulate the labeling substance, stopping irradiation with the line beam of the stimulating ray and causing the sensor to photoelectrically detect light released from the labeling substance after the completion of irradiation with the line beam of the stimulating ray. According to the thus constituted image reading apparatus, it is possible to produce low noise image data rapidly and with a simple operation by irradiating an image carrier including two-dimensionally distributed spots of a labeling substance such as a fluorescent substance, a radioactive labeling substance or the like with a stimulating ray to excite the labeling substance and photoelectrically detecting light released from the labeling substance.

Abstract translation: 一种图像读取装置适用于用包含在二维分布斑点中的标记物质的图像载体用刺激光线照射和光电检测从标记物质释放的光，从而产生图像数据，图像读取装置包括至少一个 用于将从至少一个刺激射线源发射的刺激射线成形为线束的透镜，用于光电检测从标记物质释放的光的传感器，以及用于进行刺激的控制器，以及 将包含二维分布斑点中的标记物质的图像载体用刺激射线的线束照射的刺激标记物质的停止检测步骤，停止用刺激射线的线束照射，使传感器进行光电检测 光从标签上释放出来 在用激发光束的线束照射完成后。 根据这样构成的图像读取装置，可以通过照射包含荧光物质，放射性标记物质等标记物质的二维分布的斑点的图像载体，并且通过简单的操作，可以产生低噪声图像数据。 其具有刺激性射线以激发标记物质并光电检测从标记物质释放的光。

公开(公告)号：US5108179A

公开(公告)日：1992-04-28

申请号：US391481

申请日：1989-08-09

Applicant: Stephen A. Myers

Inventor： Stephen A. Myers

IPC: G01N21/64 ,  G01N27/447

CPC classification number: G01N21/6428 ,  G01N21/645 ,  G01N27/44721 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N21/6402 ,  G01N21/6456 ,  G01N2201/064 ,  G01N2201/1042

Abstract: The use of continuous wave (CW) laser excitation to measure small differences in the fluorescence of bands of fluorophore stained nucleic acid fragments present in gels is described.

Abstract translation: 描述了使用连续波（CW）激光激发来测量存在于凝胶中的荧光团染色的核酸片段的荧光的微小差异。

公开(公告)号：EP3112844B1

公开(公告)日：2018-09-19

申请号：EP16176083.0

申请日：2016-06-24

Applicant: Agilent Technologies, Inc.

Inventor： Ghetler, Andrew ,  Kleczewski, Adam ,  Tella, Richard P.

IPC: G01N21/27 ,  G01J3/42 ,  G01N21/3563 ,  G01N21/55 ,  G01J3/02 ,  G01J3/10 ,  G01J3/28 ,  G01N21/47

CPC classification number: G01J3/0297 ,  G01J3/10 ,  G01J3/108 ,  G01J3/2823 ,  G01J3/42 ,  G01N21/274 ,  G01N21/276 ,  G01N21/3563 ,  G01N21/4785 ,  G01N21/55 ,  G01N2201/101 ,  G01N2201/1042 ,  G01N2201/127

Abstract: A method and apparatus for obtaining reference samples, i.e. measuring reference targets (62) on a reference stage (61) during the generation of a mid-infrared (MIR) image without requiring that a sample specimen (16), being placed on a specimen stage (57) and imaged, be removed is disclosed. A tunable MIR laser (11) generates a light beam (18) that is focused onto the sample specimen on the specimen stage that moves the specimen in a first direction (33). An optical assembly includes a scanning assembly (31) having a focusing lens (55) and a mirror (56) that moves in a second direction (32), different from the first direction, relative to the specimen stage. A light detector (13) measures an intensity of light leaving the point on the specimen. A controller (39) forms an image from the measured intensity. The reference stage (61) is positioned such that the scanning assembly moves over the reference stage in response to a command so that the controller can also make a reference measurement.

公开(公告)号：EP3112844A1

公开(公告)日：2017-01-04

申请号：EP16176083.0

申请日：2016-06-24

Applicant: Agilent Technologies, Inc.

Inventor： Ghetler, Andrew ,  Kleczewski, Adam ,  Tella, Richard P.

IPC: G01N21/27 ,  G01J3/42 ,  G01N21/3563 ,  G01N21/47 ,  G01N21/55

CPC classification number: G01J3/0297 ,  G01J3/10 ,  G01J3/108 ,  G01J3/2823 ,  G01J3/42 ,  G01N21/274 ,  G01N21/276 ,  G01N21/3563 ,  G01N21/4785 ,  G01N21/55 ,  G01N2201/101 ,  G01N2201/1042 ,  G01N2201/127

Abstract: A method and apparatus for obtaining reference samples, i.e. measuring reference targets (62) on a reference stage (61) during the generation of a mid-infrared (MIR) image without requiring that a sample specimen (16), being placed on a specimen stage (57) and imaged, be removed is disclosed. A tunable MIR laser (11) generates a light beam (18) that is focused onto the sample specimen on the specimen stage that moves the specimen in a first direction (33). An optical assembly includes a scanning assembly (31) having a focusing lens (55) and a mirror (56) that moves in a second direction (32), different from the first direction, relative to the specimen stage. A light detector (13) measures an intensity of light leaving the point on the specimen. A controller (39) forms an image from the measured intensity. The reference stage (61) is positioned such that the scanning assembly moves over the reference stage in response to a command so that the controller can also make a reference measurement.

Abstract translation: 一种用于获得参考样本的方法和装置，即在产生中红外（MIR）图像期间在参考台（61）上测量参考目标（62），而不需要将样本（16）放置在样本 公开了阶段（57）并被成像。 可调谐MIR激光器（11）产生聚焦在样品台上的样品样本上的光束（18），样品台沿第一方向（33）移动样本。 光学组件包括具有聚焦透镜（55）的扫描组件（31）和相对于样品台沿与第一方向不同的第二方向（32）移动的反射镜（56）。 光检测器（13）测量离开样品点的光的强度。 控制器（39）根据测量的强度形成图像。 基准台（61）被定位成使得扫描组件响应于命令移动到参考台上，使得控制器也可进行参考测量。

公开(公告)号：EP1494007B8

公开(公告)日：2014-09-24

申请号：EP04014796.9

申请日：2004-06-24

Applicant: Tecan Trading AG

Inventor： Erlbacher, Andreas ,  Niggl, Lutz ,  Krutzenbichler, Alois

IPC: G01N21/03 ,  G01N21/64 ,  G01N21/25 ,  G01N21/53 ,  G01N21/59 ,  B01L3/00

CPC classification number: G01N21/6452 ,  B01L3/5085 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/168 ,  G01N21/0303 ,  G01N21/253 ,  G01N21/59 ,  G01N2021/6463 ,  G01N2021/6491 ,  G01N2021/651 ,  G01N2201/0446 ,  G01N2201/1042 ,  Y10S436/809

公开(公告)号：EP1991860A2

公开(公告)日：2008-11-19

申请号：EP07717653.5

申请日：2007-02-23

Applicant: GENERAL ELECTRIC COMPANY

Inventor： FOMITCHOV, Pavel A. ,  BARASH, Eugene ,  YEKTA, Ahmad ,  MASINO, Joseph

IPC: G01N23/00

CPC classification number: G01N21/6458 ,  G01N21/47 ,  G01N2201/0407 ,  G01N2201/1042 ,  G02B21/0048 ,  G02B21/0076 ,  G02B21/367

Abstract: A system and method for multimode imaging of at least one sample is disclosed. The system includes at least one light source; an optical system selected responsive to a mode of operation of the imaging system; and a detector capable of selective reading of pixels. The at least one sample is moved relative to the optical system using a sample movement technique selected from the group consisting of step sample moving and continuous sample moving. The method includes the steps of (1) selecting a mode of operation for the imaging system; (2) transmitting light from at least one light source through an optical system selected in response to the mode of operation for the imaging system; (3) moving the at least one sample relative to the optical system using a sample movement technique selected from the group consisting of step sample moving and continuous sample moving; and (4) selectively reading pixels with a detector.