公开(公告)号：KR1020110088102A

公开(公告)日：2011-08-03

申请号：KR1020100007846

申请日：2010-01-28

Applicant: 가톨릭대학교 산학협력단

Inventor： 박용일 ,  박제권 ,  김우중

IPC: C12N1/20 ,  C12P19/04 ,  C12R1/39

Abstract: PURPOSE: A Pseudomonas fluorescens JK-0412 with a chitin decomposition ability and a method for producing N-acetylglucosamine(GlcNAc) using the same are provided to ensure cheap synthesis. CONSTITUTION: A Pseudomonas fluorescens JK-0412(deposit number KCTC 11623BP) has an ability of decomposing chitin. The strain produces N-acetylglucosaminidase from a culture medium by culturing the strain in the medium. A method for producing N-acetylglucosaminidase comprises a step of adding chitin to the medium. N-acetylglucosaminidase is contained in the supernatant of the culture medium in which the strain is vaccinated. The chitin is a powdery or colloidal chitin. The strain is cultured at 25-50°C and pH 6-8.

Abstract translation: 目的：提供具有甲壳素分解能力的荧光假单胞菌JK-0412和使用其的生产N-乙酰葡糖胺（GlcNAc）的方法以确保廉价的合成。 构成：荧光假单胞菌JK-0412（保藏号KCTC 11623BP）具有分解壳多糖的能力。 该菌株通过在培养基中培养菌株从培养基中产生N-乙酰氨基葡糖苷酶。 制备N-乙酰氨基葡糖苷酶的方法包括向培养基中加入壳多糖的步骤。 N-乙酰氨基葡糖苷酶包含在其中接种菌株的培养基的上清液中。 几丁质是粉状或胶体壳多糖。 该菌株在25-50℃和pH 6-8下培养。

公开(公告)号：KR1020110014820A

公开(公告)日：2011-02-14

申请号：KR1020090072366

申请日：2009-08-06

Applicant: 가톨릭대학교 산학협력단

Inventor： 박용일 ,  나예슬 ,  김우중

IPC: A61K31/715 ,  A61K36/05 ,  A61P7/02 ,  A61P29/00

CPC classification number: A61K31/737 ,  A61K36/05 ,  A61K2236/53 ,  Y10S514/822 ,  Y10S514/886

Abstract: PURPOSE: A polysaccharide extracted from Capsosiphon fulvescens with anti-thrombotic effect and immunity is provided to produce a large amount of polysaccharide without side effects. CONSTITUTION: A polysaccharide having anti-thrombotic effect and immunity is isolated from Capsosiphon fulvescens. The polysaccharide is a soluble polysaccharide containing sulfate residue. The polysaccharide contains mannose and galactose as a main ingredient. A composition contains the polysaccharide as an active ingredient. A method for preparing the polysaccharide comprises: a step of neutralizing hydrolyzed extract of Capsosiphon fulvescens; a step of precipitating with alcohol of 1-4 carbon atoms, ethyl acetate, hexane, dichloromethane, or mixture solvent thereof and centrifuging to obtain a first precipitate; a step of treating the first precipitate with calcium chloride(CaCl_2) and centrifuging to obtain a supernatant; a step of treating the supernatant with alcohol of 1-4 carbon atoms, ethyl acetate, hexane, dichloromethane, or mixture solvent thereof to obtain a second precipitate; and a step of dialyzing the second precipitate and drying.

Abstract translation: 目的：提供具有抗血栓形成作用和免疫力的辣椒素提取的多糖，以产生大量的多糖，无副作用。 构成：从Capsosiphon fulvescens中分离出具有抗血栓形成作用和免疫力的多糖。 多糖是含有硫酸盐残基的可溶性多糖。 多糖含有甘露糖和半乳糖作为主要成分。 组合物含有多糖作为活性成分。 制备多糖的方法包括：中和辣椒皂苷的水解提取物的步骤; 用1-4个碳原子的醇沉淀的步骤，乙酸乙酯，己烷，二氯甲烷或其混合溶剂，并离心得到第一沉淀物; 用氯化钙（CaCl 2）处理第一沉淀物并离心以获得上清液的步骤; 用1-4个醇的醇处理上清液的步骤，乙酸乙酯，己烷，二氯甲烷或其混合溶剂，得到第二沉淀物; 和透析第二沉淀物并干燥的步骤。

公开(公告)号：KR1020080104679A

公开(公告)日：2008-12-03

申请号：KR1020070051771

申请日：2007-05-29

Applicant: 가톨릭대학교 산학협력단

Inventor： 박용일 ,  김우중 ,  김현걸 ,  김성민 ,  정미경 ,  박주웅 ,  서현효

IPC: C12N1/20 ,  C12N1/00

Abstract: A novel Sphingomonas sp strain, a method for culturing the strain, and a method for preparing fuco-oligosaccharide by using the strain are provided to improve the degradation of fucoidan of brown algae into fuco-oligosaccharide. A Sphingomonas sp strain contains an enzyme which can degrade the fucoidan of brown algae into fuco-oligosaccharide. Preferably the strain belongs to the Sphingomonas paucimobilis sp. The deposition number of the strain is KCTC 11130BP.

Abstract translation: 提供新型鞘氨醇单胞菌菌株，培养菌株的方法以及通过使用该菌株制备非低聚糖的方法，以改善褐藻酸的岩藻依聚糖降解为低聚果糖。 鞘氨醇单胞菌属菌株含有可将褐藻酸岩藻依聚糖降解成低聚果糖的酶。 优选地，菌株属于矮化型鞘氨醇单胞菌（Sphingomonas paucimobilis sp。 菌株的沉积次数为KCTC 11130BP。