Abstract:
PURPOSE: A pPLim3 promoter specifically expressed in pollen and a recombinant expression vector containing the same are provided to induce expression of useful gene in pollen tissue and to develop transformant of novel species. CONSTITUTION: A pPLim3 promoter for inducing specific expression of pollen tissue has a base sequence of sequence number 1. A structure gene is specifically expressed in pollen tissue of a plant. A recombinant expression vector contains the pPLim3 promoter and structure gene which is operably linked with the promoter. The vector induces expression of the structure gene in pollen tissue. The vector additionally contains a structure gene, operator, polyadenylation signal, enhancer, secretion signal, selection marker, or replication origin.
Abstract:
PURPOSE: A gene related to seed size and growth isolated from Oryza sativa is provided to develop rice seed having high quality. CONSTITUTION: An OsLIM gene which is related to seed size and growth of rice plants comprise a nucleotide sequence of sequence number 1. A transgenic vector pMJ-Cytc+LIM contains the OsLIM gene of sequence number 1. The rice transfomant is obtained by transforming with Argrobacterium. A method for preparing the rice transformant comprises: a step of constructing transforming actor by cloning OsLIM gene with Topo vector; a step of introducing the transformed vector to the host cells.
Abstract:
PURPOSE: Provided are plant tissue specific promoters, thereby controlling when, where and how much to express a foreign gene. CONSTITUTION: A pollen specific promoter region Pban102 contains 1,177 bp upstream of an initiation codon ATG in a genomic clone of BAN102 gene having the nucleotide sequence of SEQ ID NO: 1. A pollen superior promoter region Pban103 contains 1 to 1831 bp in a genomic clone of BAN103 gene having the nucleotide sequence of SEQ ID NO: 2. A pollen specific promoter region P84 contains 1 to 565 bp in a genomic clone of BAN84 gene having SEQ ID NO: 3. Plasmids, pGBAN84, p84Xb, p84Bm and p84SM, contain the genomic clone of Chinese cabbage containing the BAN84 gene. A carpet tissue specific promoter region PBcA9 contains 616 to 1,436 bp in a genomic clone of BcA9 gene having the nucleotide sequence of SEQ ID NO: 4. A vascular bundle specific promoter region Pbif38 contains 1 to 966 bp in a gemonic clone of BIF38 gene having the nucleotide sequence of SEQ ID NO: 5.
Abstract translation:目的:提供植物组织特异性启动子,从而控制表达外源基因的时间,位置和数量。 构成:花粉特异性启动子区域Pban102在具有SEQ ID NO:1的核苷酸序列的BAN102基因的基因组克隆中含有起始密码子ATG上游1177bp。花粉优势启动子区域Pban103在基因组克隆中含有1至1831bp 具有SEQ ID NO:2的核苷酸序列的BAN103基因。花粉特异性启动子区P84在具有SEQ ID NO:3的BAN84基因的基因组克隆中含有1至565bp。质粒pGBAN84,p84Xb,p84Bm和p84SM含有 含有BAN84基因的大白菜的基因组克隆。 地毯组织特异性启动子区域PBcA9在具有SEQ ID NO:4的核苷酸序列的BcA9基因的基因组克隆中含有616至1,436bp。血管束特异性启动子区域Pbif38在BIF38基因的宝石克隆中含有1至966bp, SEQ ID NO:5的核苷酸序列。
Abstract:
본 발명은 식물의 특정한 생장 발달 시기에 특정한 조직에서 유전자의 발현을 특이적으로 유도할 수 있는 신규한 프로모터에 관한 것이다. 보다 구체적으로, 본 발명은 식물의 유묘 발달 시기에 줄기 정단 분열조직, 잎의 주맥을 포함하는 잎맥, 배수조직 및 주근에서 유전자의 발현을 특이적으로 유도하며, 식물의 개화 및 종자 성숙 시기에 꽃받침, 암술대 및 꼬투리의 말단 조직에서 유전자의 발현을 특이적으로 유도할 수 있는 신규한 BrLRP1 프로모터에 관한 것이다. 본 발명에 따르면 식물의 유묘 발달 시기에 줄기 정단 분열조직, 잎의 주맥을 포함하는 잎맥, 배수조직 및 주근에서 유전자의 발현을 특이적으로 유도하고, 식물의 개화 및 종자 성숙 시기에 꽃받침, 암술대 및 꼬투리의 말단 조직에서 유전자의 발현을 특이적으로 유도할 수 있는 형질전환 식물을 제조할 수 있다. 따라서 식물의 생장 발달 단계 중 상기 특정 조건 하에서 유전자 생성물의 효율적인 생산을 위해 유용하게 활용될 수 있다.
Abstract:
본 발명은 배추(Brassica rapa)에서 분리한 기능이 아직 알려져 있지 않은 유전자 BrPSR(Brassica rapa Plant Size Regulation)의 식물 생장 촉진 용도에 관한 것으로서, 상기 유전자를 식물체 내로 도입하여 세포내에서 발현시킴으로써 식물의 생장을 촉진시킬 수 있다. 보다 구체적으로 본 발명은 배추에서 분리한 BrPSR 유전자의 식물 생장 촉진 용도, 상기 유전자가 도입된 형질전환 식물체 및 상기 유전자를 식물체에 도입시켜 식물의 생장을 촉진시키는 방법에 관한 것이다. 본 발명은 식물체 전체 크기, 잎, 꽃, 종자 크기를 증가시켜 식물의 생장을 촉진시키고자 할 경우, 특히 관상 목적으로 식물의 크기, 꽃의 크기를 크게 하거나, 잎, 꽃, 종자의 크기를 크게 하여 생산성을 높이고자 하는 경우에 유용하게 사용할 수 있을 것이다.
Abstract:
PURPOSE: A transformant which produces rotavirus VP7 antigen protein is provided to induce antibody formation with respect to rotavirus in human body using a vaccine, and to prevent rotavirus disease. CONSTITUTION: An expression vector contains VP7 antigen gene encoding rotavirus VP7 coat protein in sequence number 1. VP7 antigen gene has a nucleic acid sequence in sequence number 2. An expression vector is VP7/pB7WG2D vector. An Agrobacterium sp. microorganism is transformed by the expression vector. A transformant is prepared by the microorganism. A recombinant rotavirus VP7 antigen protein is expressed from the transformant. A vaccine composition for oral administration for preventing and treating rotavirus diseases contains the transformant, a protein extract of the transformant, or the recombinant rotavirus VP7 antigen protein as an active ingredient. [Reference numerals] (AA) BP reaction; (BB) LP reaction
Abstract:
PURPOSE: A recombinant vector and a transformed cell are provided to obtain a transgenic plant with high zeaxanthin content, to improve visual acuity, and to obtain a gene and carrier IP for developing a novel functional color GM(genetic modified) crop material. CONSTITUTION: A polynucleotide is denoted by sequence number 3 or 4. A recombinant vector containing the polynucleotide is pGlb:Bch or pGlb:stBch. A transformed cell is prepared by transforming with the vector and includes Agrobacterium sp. microorganism or a plant cell. A transgenic plant is prepared by introducing the vector. A transgenic rice plant contains the vector. [Reference numerals] (AA) Normal rice; (BB) Beta carotene rice; (CC) Zeaxanthin rice; (DD) Recombination gene name; (EE) Patent No. 10-0905219; (FF) Reference
Abstract:
PURPOSE: A BRLRP1 promoter which is specific to a plant tissue is provided to specifically induce gene expression in a certain tissue and to effectively produce a gene product under a predetermined condition. CONSTITUTION: A promoter contains a nucleic acid sequence of sequence number 1. An expression vector, pPBrLRP1 vector, contains the promoter. A cell is transformed with the expression vector and includes Agrobacterium sp. microorganism or a plant cell. A method for producing a transgenic plant comprises: a step of transforming a plant cell with the expression vector; and a step of re-differentiating the transgenic plant prepared from the transformed plant cell.