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    심해 해저에서 분리된 에스터라제 ＫＴＬ 9
    62.
    发明公开
    심해 해저에서 분리된 에스터라제 ＫＴＬ 9 有权
    从深海沉积物中分离的酯酶KTL 9

    公开(公告)号：KR1020120129852A

    公开(公告)日：2012-11-28

    申请号：KR1020120116123

    申请日：2012-10-18

    Applicant: 한국해양과학기술원

    Inventor： 이정현 ,  이현숙 ,  강성균 ,  김윤재 ,  배승섭 ,  전정호 ,  임재규 ,  김상진 ,  권개경 ,  차선신

    IPC: C12N9/16 ,  C12N15/55 ,  C12N15/63

    Abstract: PURPOSE: An esterase KTL9 which is separated from the bottom of the sea is provided to enhance activities and to have stability in wide range of temperature. CONSTITUTION: An esterase KTL9 which is separated from the bottom of the sea has amino acid sequence of the sequence number 34. A gene ciphering the esterase has the base sequence described in the sequence number 33. A manufacturing method of the esterase gene comprises the following steps: transforming the cells into recombinant vector which includes the esterase; culturing the transformed cells; and separating the esterase from the cultured cell.

    Abstract translation: 目的：提供与海底分离的酯酶KTL9，以增强活性并在宽温度范围内具有稳定性。 构成：从海底分离的酯酶KTL9具有序列号34的氨基酸序列。加密酯酶的基因具有序列号33中所述的碱基序列。酯酶基因的制造方法包括以下 步骤：将细胞转化成包含酯酶的重组载体; 培养转化细胞; 并从培养的细胞中分离酯酶。

    써모코쿠스 속 균주를 이용한 수소 가스 생산 방법
    63.
    发明公开
    써모코쿠스 속 균주를 이용한 수소 가스 생산 방법 无效
    使用热电偶生产氢气的方法

    公开(公告)号：KR1020120103238A

    公开(公告)日：2012-09-19

    申请号：KR1020110021390

    申请日：2011-03-10

    Applicant: 한국해양과학기술원

    Inventor： 강성균 ,  이정현 ,  이현숙 ,  권개경 ,  임형순 ,  김윤재 ,  배승섭 ,  임재규 ,  김민식

    IPC: C12P3/00 ,  C12P1/04 ,  C01B3/02 ,  C12R1/01

    CPC classification number: C12P3/00

    Abstract: PURPOSE: A method for producing hydrogen gas(H_2) using Thermococcus sp. strain is provided to generate hydrogen of high yield and high purity without harmful by-product. CONSTITUTION: A method for producing hydrogen gas(H_2) using Thermococcus sp. strain comprises: a step of culturing the strain in a medium containing carbon monoxide, formic acid or a salt thereof, or starch at 50-90 Deg.C.; and a step of collecting hydrogen gas. The Thermococcus sp. strain is Thermococcus gammatolerans, Thermococcus barophilus Ch5, Thermococcus sp. DS-1, Thermococcus sp. DT-4, Thermococcus barophilus MP, or Thermococcus sp. AM4.

    Abstract translation: 目的：使用Thermococcus sp。生产氢气（H_2）的方法。 提供应变以产生高产率和高纯度的氢气而没有有害的副产物。 构成：使用Thermococcus sp。生产氢气（H_2）的方法。 菌株包括：在50-90℃下在含有一氧化碳，甲酸或其盐或淀粉的培养基中培养菌株的步骤。 以及收集氢气的步骤。 热球菌属 菌株是Thermococcus gammatolerans，Thermococcus barophilus Ch5，Thermococcus sp。 DS-1，Thermococcus sp。 DT-4，Thermococcus barophilus MP或Thermococcus sp。 AM4。

    ＤＮＡ 중합효소 Ｔ7 및 이의 유전자들
    64.
    发明公开
    ＤＮＡ 중합효소 Ｔ7 및 이의 유전자들 无效
    DNA聚合酶T7及其基因

    公开(公告)号：KR1020110126011A

    公开(公告)日：2011-11-22

    申请号：KR1020100045691

    申请日：2010-05-14

    Applicant: 한국해양과학기술원

    Inventor： 이정현 ,  강성균 ,  김상진 ,  권개경 ,  이현숙 ,  김윤재 ,  배승섭 ,  임재규 ,  전정호 ,  조요나 ,  황영옥 ,  차선신

    IPC: C12N9/12 ,  C12N15/54 ,  C12N15/63

    Abstract: PURPOSE: A DNA polymerase T7 and genes thereof are provided to ensure processivity, fidelity, and elongation length and to be applied in various fields. CONSTITUTION: A DNA polymerase T7 has an amino acid sequence of sequence number 2. A DNA polymerase T7 gene encodes an amino acid sequence of sequence number 2. The DNA polymerase T7 gene contains a base sequence of sequence number 1. A recombinant vector contains the T7 gene. The polymerase T7 is prepared by culturing a host cells transformed by the recombinant vector, inducing the recombinant protein expression, and isolating polymerase protein. The recombinant vector contains the DNA polymerase T7 gene. The DNA polymerase T7 is derived from Thermococcus sp. NA1.

    Abstract translation: 目的：提供DNA聚合酶T7及其基因，以确保持续性，保真度和伸长率，并适用于各种领域。 构成：DNA聚合酶T7具有序列号2的氨基酸序列.DNA聚合酶T7基因编码序列号2的氨基酸序列.DNA聚合酶T7基因含有序列号1的碱基序列。重组载体含有 T7基因。 通过培养由重组载体转化的宿主细胞，诱导重组蛋白质表达和分离聚合酶蛋白来制备聚合酶T7。 重组载体含有DNA聚合酶T7基因。 DNA聚合酶T7衍生自Thermococcus sp。 NA1。

    단백질분해효소 및 이를 코딩하는 유전자
    65.
    发明公开
    단백질분해효소 및 이를 코딩하는 유전자 无效
    编码它们的基因和基因

    公开(公告)号：KR1020090063644A

    公开(公告)日：2009-06-18

    申请号：KR1020070131091

    申请日：2007-12-14

    Applicant: 한국해양과학기술원

    Inventor： 김상진 ,  강성균 ,  권개경 ,  이정현 ,  강지현

    IPC: C12N9/50 ,  C12N9/00

    Abstract: A protease and gene coding the same are provided to ensure excellent algicidal activity and usefully prevent the red tide. A protease comprises amino acids of sequence number 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137 or 139. A polynucleotide coding the protease comprises base of the sequence number 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138 or 140. The protease is secreted from a kordia algicida OT-1(KCTC 8814P) strain which kills a skeletonema costatum.

    Abstract translation: 提供了一种蛋白酶及其编码基因，以确保优异的杀藻活性，有效防止红潮。 一种蛋白酶包含序列号1,3,5,7,9,11,13,15,17,19,21,23,25,27,29,31,33,35,37,39,41,46,33,39,39,39,39,39,39,31,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39 43，45，47，49，51，53，55，57，59，61，63，65，67，69，71，73，75，77，79，81，83，85，87，89， 93,95,97,99,101,103,105,107,109,111,113,115,117,119,121,123,125,127,129,131,133,135,137或139.一种多核苷酸 编码蛋白酶的步骤包括序列号2,4,6,8,10,12,14,16,18,20,22,24,26,28,30,32,34,36,38,40,42的碱基 ，44，46，48，50，52，54，56，58，60，62，64，66，68，70，72，74，76，78，80，82，84，86，88，90，92 ，94,96,98,100,102,104,106,108,110,112,114,116,118,120,122,124,126,128,130,132,134,136,138或140中。 蛋白酶是从一个kordia algicida OT-1（KCTC 8814P）菌株分泌的，该菌株杀死骨骼水肿。

    심해 해저에서 분리된 에스터라제 KTL 4
    66.
    发明公开
    심해 해저에서 분리된 에스터라제 KTL 4 有权
    从深海海域隔离的食物和食物

    公开(公告)号：KR1020090038757A

    公开(公告)日：2009-04-21

    申请号：KR1020070104219

    申请日：2007-10-16

    Applicant: 한국해양과학기술원

    Inventor： 이정현 ,  이현숙 ,  강성균 ,  조요나 ,  김윤재 ,  배승섭 ,  전정호 ,  임재규 ,  김상진 ,  권개경 ,  차선신 ,  김준태 ,  김형권

    IPC: C12N9/20 ,  C12N9/18 ,  C12N9/00

    Abstract: Provided is lipase and esterase isolated from deep sea mass-produced by genes coding the lipase and esterase. An esterase gene codes esterase having amino acid sequence of SEQ ID:NO 2, 4, 6, 10, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32 or 34. A method for preparing the esterase comprises the following steps of: cultivating a transformed cell with a recombinant vector; and isolating the esterase from the cultivated cells. The recombinant vector includes sequence number of 1, 3, 5, 9, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31 or 33. The lipase gene codes lipase having amino acid sequence of SEQ ID:NO 8 or 12. The method for preparing the lipase consists of the following steps of: cultivating a transformed cell with a recombinant vector; and isolating the lipase from the cultivated cells. The recombinant vector has sequence number of 7 or 11.

    Abstract translation: 提供从通过编码脂肪酶和酯酶的基因产生的深海分离的脂肪酶和酯酶。 酯酶基因编码具有SEQ ID NO：2,4,6,10,14,16,18,20,22,24,26,28,30,32或34的氨基酸序列的酯酶。制备酯酶的方法 包括以下步骤：用重组载体培养转化的细胞; 并从培养的细胞中分离酯酶。 重组载体包含序列号1,3,5,9,13,15,17,19,21,23,25,27,29,31或33.脂肪酶基因编码具有SEQ ID NO：1的氨基酸序列的脂肪酶， NO 8或12.制备脂肪酶的方法包括以下步骤：用重组载体培养转化的细胞; 并从培养的细胞中分离脂肪酶。 重组载体的序列号为7或11。

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