公开(公告)号：BRPI0417710A

公开(公告)日：2007-03-20

申请号：BRPI0417710

申请日：2004-12-15

Applicant: BASF AG

Inventor： KRUEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGER CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

IPC: C07K14/34 ,  C12N20060101 ,  C12N1/00 ,  C12N15/63 ,  C12N15/67 ,  C12N15/77 ,  C12P1/00 ,  C12P13/08 ,  C12P13/12 ,  C07K14/195 ,  C12N15/74 ,  C12P13/00

Abstract: Use, for transcribing genes, of a nucleic acid (I) with promoter activity, where (I) is a 186 base pair sequence (1), reproduced; a variant of (1) with at least 90% identity and derived by substitution, insertion or deletion of nucleotides; a sequence that hybridizes to (1) under stringent conditions; or a functionally equivalent fragment of them, is new. Independent claims are also included for the following: (1) use of an expression unit (EU), containing (I) and functionally linked to a sequence (X) that ensures translation of RNA, for expressing genes; (2) (I), other than sequence (1), i.e. (Ia), as new compounds; (3) EU that contain (Ia) linked to (X); (4) altering (or producing) the transcription rate of genes in a microorganism, relative to the wild type; (5) expression cassette (EC) comprising at least one EU of (1), at least one other nucleic acid sequence (to be expressed) and optionally additional gene control elements, where at least the first two are linked and the sequence being expressed is heterologous with respect to EU; (6) expression vector (EV) that contains EC; (7) genetically modified microorganisms (GMO) having, for at least one gene, an altered (or induced) transcription rate, relative to the wild type; (8) preparing biosynthetic products by culturing GMO of (7); (9) use of the sequence aggagga (42) as a ribosome-binding site in expression units that provide expression of genes in Corynebacterium or Brevibacterium; (10) use of the sequences tagttt (39), taggat (40) or tgcgct (41) as -10 regions for expression of genes in Corynebacterium or Brevibacterium; and (11) expression units that contain sequences (39)-(42).

公开(公告)号：NO20062695A

公开(公告)日：2006-09-12

申请号：NO20062695

申请日：2006-06-12

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  HAEFNER STEFAN ,  SCHRODER HARTWIG ,  KROGER BURKHARD ,  RUFFER UWE ,  GRAEF CLAUDIA ISABELLA ,  HABERHAUER GREGOR

IPC: C07K14/34

CPC classification number: C07K14/34

公开(公告)号：AU2005321577A1

公开(公告)日：2006-07-06

申请号：AU2005321577

申请日：2005-12-21

Applicant: BASF AG

Inventor： HAEFNER STEFAN ,  SCHRODER HARTWIG ,  HEROLD ANDREA ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA

IPC: C12N15/77 ,  C07K14/34

Abstract: An expression unit (A) that contains several promoters is new. A new expression unit (A) comprises several promoters and includes, in the 5' to 3' direction the sequence module (I). 5'-P 1-(A x-P x) n-A y-P y-3' (I) n = integer 0-10; A x and A y = same or different chemical bonds or nucleic acid linker sequences; P 1, P x and P y = same or different promoter sequences, containing at least one RNA-polymerase binding region, and at least P y also includes a ribosome-binding, 3'-terminal segment. Independent claims are also included for: (1) an expression cassette (EC) that contains, in the 5' to 3' direction, the sequence module (II); (2) vector that contains at least one EC; (3) a genetically modified microorganism (GMO) transfected with the vector of (2) or containing EC; and (4) method for preparing biosynthetic products (X) by culturing GMO. 5'-P 1-(A x-P x) n-A y-P y-G-3' (II) G : at least one nucleic acid coding sequence functionally linked to the 5'-upstream regulatory sequence.

公开(公告)号：DE102004035052A1

公开(公告)日：2006-02-16

申请号：DE102004035052

申请日：2004-07-20

Applicant: BASF AG

Inventor： SAUER UWE ,  MAMPEL JOERG ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  ZELDER OSKAR ,  HEROLD ANDREA ,  KLOPPROGGE CORINNA

IPC: C12N1/00

Abstract: The present invention relates to microorganisms and methods for producing at least one sulfur-containing compound.

公开(公告)号：AR047151A1

公开(公告)日：2006-01-11

申请号：ARP040104736

申请日：2004-12-17

Applicant: BASF AG

Inventor： KROEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

IPC: F01D15/10 ,  F02C3/113 ,  F02C7/268 ,  C12N15/31 ,  C12N15/67 ,  C12N15/63 ,  C12N15/77 ,  C12N1/21 ,  C12P13/08 ,  C12P13/12 ,  C12R1/15 ,  C12R1/13

Abstract: Se refiere al uso de secuencias de ácidos nucleicos para la regulacion de la transcripcion y la expresion de genes, a los nuevos promotores y unidades de expresion propiamente dichos, a procedimientos para la modificacion o activacion del grado de transcripcion y/o grado de expresion de genes, a casetes de expresion que contienen las unidades de expresion, a microorganismos modificados genéticamente con grado de transcripcion y/o grado de expresion modificado o activado, así como también a procedimientos para la preparacion de productos biosintéticos por medio del cultivo de los microorganismos genéticamente modificados. Reivindicacion 1: Uso de un ácido nucleico con actividad promotora, que contiene: A) la secuencia de ácidos nucleicos SEQ. ID. NO. 1 o B) una secuencia derivada de esta secuencia por sustitucion, insercion o delecion de nucleotidos, la que presenta una identidad de por lo menos 90 % a nivel de ácidos nucleicos con la secuencia SEQ. ID. NO. 1, o C) una secuencia de ácidos nucleicos, que hibrida con la secuencia de ácidos nucleicos SEQ. ID. NO. 1 bajo condiciones severas o D) fragmentos funcionalmente equivalentes de las secuencias de A), B) o C), para la transcripcion de genes.

公开(公告)号：AR047057A1

公开(公告)日：2006-01-04

申请号：ARP040104740

申请日：2004-12-17

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  KROEGER BURKHARD ,  KIEFER PATRICK ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH

IPC: A61K38/15 ,  G01N33/574 ,  C12N15/53 ,  C12N1/21 ,  C12P13/08 ,  C12R1/15

Abstract: Comprende métodos para aumentar la produccion de un compuesto químico fino, por ejemplo, lisina a partir de un microorganismo, por ejemplo, Corynebacterium mediante la desregulacion de un gen codificador de una enzima, es decir, lactato deshidrogenasa. Se proveen métodos para incrementar la produccion de lisina en Corynebacterium glutamicum a través del incremento de la expresion de la actividad de lactato deshidrogenasa. También se provee un proceso para la produccion de lisina a través de la regulacion del flujo de carbono hacia oxalacetato (OAA).Se proveen métodos para la produccion de lisina a través de la utilizacion de fructosa o sacarosa como fuente de carbono. Reivindicacion 1: Un método para aumentar el flujo metabolico a través de la vía de pentosafosfatos en un microorganismo que comprenda el cultivo de dicho microorganismo que contenga un gen que se desregula bajo condiciones tales que se aumenta el flujo metabolico a través de la vía de pentosafosfatos. Reivindicacion 49: Un microorganismo recombinante que comprende un gen de biosíntesis de pentosafosfato desregulado

公开(公告)号：BR0215958A

公开(公告)日：2005-09-13

申请号：BR0215958

申请日：2002-11-29

Applicant: BASF AG

Inventor： POMPEJUS MARKUS ,  GER BURKHARD KR ,  DER HARTWIG SCH ,  ZELDER OSKAR ,  HABERHAUER GREGOR ,  HAEFNER STEFAN ,  KLOPPROGGE CORINNA

IPC: C07K14/34 ,  C12N15/31 ,  C12N15/77 ,  C12P13/12 ,  C12R1/13 ,  C12R1/15

公开(公告)号：AU2004298546A1

公开(公告)日：2005-06-30

申请号：AU2004298546

申请日：2004-12-15

Applicant: BASF AG

Inventor： HAEFNER STEFAN ,  KLOPPROGGE CORINNA ,  SCHRODER HARTWIG ,  KROGER BURKHARD ,  ZELDER OSKAR

IPC: C12P13/08 ,  C07K14/34 ,  C12N20060101 ,  C12N1/00 ,  C12N15/63 ,  C12N15/67 ,  C12N15/77 ,  C12P1/00 ,  C12P13/12

Abstract: Use, for transcribing genes, of a nucleic acid (I) with promoter activity, where (I) is a 186 base pair sequence (1), reproduced; a variant of (1) with at least 90% identity and derived by substitution, insertion or deletion of nucleotides; a sequence that hybridizes to (1) under stringent conditions; or a functionally equivalent fragment of them, is new. Independent claims are also included for the following: (1) use of an expression unit (EU), containing (I) and functionally linked to a sequence (X) that ensures translation of RNA, for expressing genes; (2) (I), other than sequence (1), i.e. (Ia), as new compounds; (3) EU that contain (Ia) linked to (X); (4) altering (or producing) the transcription rate of genes in a microorganism, relative to the wild type; (5) expression cassette (EC) comprising at least one EU of (1), at least one other nucleic acid sequence (to be expressed) and optionally additional gene control elements, where at least the first two are linked and the sequence being expressed is heterologous with respect to EU; (6) expression vector (EV) that contains EC; (7) genetically modified microorganisms (GMO) having, for at least one gene, an altered (or induced) transcription rate, relative to the wild type; (8) preparing biosynthetic products by culturing GMO of (7); (9) use of the sequence aggagga (42) as a ribosome-binding site in expression units that provide expression of genes in Corynebacterium or Brevibacterium; (10) use of the sequences tagttt (39), taggat (40) or tgcgct (41) as -10 regions for expression of genes in Corynebacterium or Brevibacterium; and (11) expression units that contain sequences (39)-(42).

公开(公告)号：BRPI0612900A2

公开(公告)日：2014-10-14

申请号：BRPI0612900

申请日：2006-07-18

Applicant: BASF AG

Inventor： HAEFNER STEFAN ,  HEROLD ANDREA ,  KLOPPROGGE CORINNA ,  YOCUM R ROGERS ,  ZELDER OSKAR ,  SCHROEDER HARTWIG ,  WILLIAMS MARK K

IPC: C12P13/12

Abstract: The present invention features improved processes and organisms for the production of methionine. The invention demonstrates that a &Dgr;metF organism or a &Dgr;metE AmetH organism, for example, mutants of C. glutamicum or E. coli, can use a methyl capped sulfide source, e.g., dimethyl disulfide (DMDS), as a source of both sulfur and a methyl group, bypassing the need for MetH/MetE and MetF activity and the need to reduce sulfate, for the synthesis of methionine. Also described in this patent are data implicating MetY (also called MetZ) as an enzyme that incorporates a methyl capped sulfide source, e.g., DMDS, into methionine. A &Dgr;metF &Dgr;metB strain of C. glutamicum can use a methyl capped sulfide source, e.g., DMDS, as a source of both sulfide and a methyl group. Furthermore, methionine production by engineered prototrophic organisms that overproduce O-acetyl-homoserine was improved by the addition of a methyl capped sulfide source, e.g., DMDS.

公开(公告)号：BRPI0520842A2

公开(公告)日：2012-07-03

申请号：BRPI0520842

申请日：2005-12-21

Applicant: BASF AG

Inventor： HAEFNER STEFAN ,  SCHROEDER HARTWIG ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  HEROLD ANDREA

IPC: C12N15/77 ,  C07K14/34

Abstract: An expression unit (A) that contains several promoters is new. A new expression unit (A) comprises several promoters and includes, in the 5' to 3' direction the sequence module (I). 5'-P 1-(A x-P x) n-A y-P y-3' (I) n = integer 0-10; A x and A y = same or different chemical bonds or nucleic acid linker sequences; P 1, P x and P y = same or different promoter sequences, containing at least one RNA-polymerase binding region, and at least P y also includes a ribosome-binding, 3'-terminal segment. Independent claims are also included for: (1) an expression cassette (EC) that contains, in the 5' to 3' direction, the sequence module (II); (2) vector that contains at least one EC; (3) a genetically modified microorganism (GMO) transfected with the vector of (2) or containing EC; and (4) method for preparing biosynthetic products (X) by culturing GMO. 5'-P 1-(A x-P x) n-A y-P y-G-3' (II) G : at least one nucleic acid coding sequence functionally linked to the 5'-upstream regulatory sequence.