흡열반응을 통한 가열-냉각 과정에 의한 세포 용해 방법
    71.
    发明授权
    흡열반응을 통한 가열-냉각 과정에 의한 세포 용해 방법 有权
    通过吸热反应进行热冷却的细胞溶解法

    公开(公告)号:KR100601982B1

    公开(公告)日:2006-07-18

    申请号:KR1020050005537

    申请日:2005-01-20

    Abstract: 본 발명은, 용해하고자 하는 세포 샘플을 준비하는 단계; 세포 샘플의 온도를 높이는 가열 단계; 및 세포 샘플 주위에서 흡열 반응을 일으킴으로써 세포 샘플의 온도를 낮추는 냉각 단계를 포함하는 세포의 용해 방법을 제공한다.
    본 발명의 방법에 의하면, 별도의 에너지원이 필요하지 않고 운반이 가능하므로, 장소나 기기에 구애를 받지 않고, 간편하게 세포 용해를 수행할 수 있으며, 특히 시료의 양이 적은 바이오칩 내에서 세포 용해를 행할 경우 그 효과는 더욱 크다 할 수 있다. 또한 세포 용해 효율도, 단순히 가열만을 행한 경우에 비하여, 훨씬 우수한 효율을 나타낸다.

    Abstract translation: 本发明提供了制备细胞样品的方法,包括:制备要溶解的细胞样品; 提高细胞样品温度的加热步骤; 以及通过在细胞样本周围引起吸热反应来降低细胞样本的温度的冷却步骤。

    B형 간염 검사용 PCR 프라이머 세트 및 이를 포함하는 B형 간염 검사 키트
    73.
    发明公开
    B형 간염 검사용 PCR 프라이머 세트 및 이를 포함하는 B형 간염 검사 키트 有权
    用于检测乙型肝炎病毒的PCR检测装置将检测时间减少到30分钟,并提高检测重现性和用于检测乙型肝炎病毒B的方法

    公开(公告)号:KR1020050018511A

    公开(公告)日:2005-02-23

    申请号:KR1020030056432

    申请日:2003-08-14

    CPC classification number: C12Q1/706 C12Q2565/629 C12Q2565/543 C12Q2531/113

    Abstract: PURPOSE: A PCR primer set for detection of hepatitis B and a method for detecting hepatitis B using the same primer set are provided, thereby reducing the detection time to 30 minutes and improving the detection reproductivity by optimizing the PCR condition and using specific PCR primer set. CONSTITUTION: The PCR primer set for detection of hepatitis B is selected from the group consisting of: (a) a set of primers having the nucleotide sequences of SEQ ID NO:1 and SEQ ID NO:2; (b) a set of primers having the nucleotide sequences of SEQ ID NO:1 and SEQ ID NO:3; (c) a set of primers having the nucleotide sequences of SEQ ID NO:4 and SEQ ID NO:5; (d) a set of primers having the nucleotide sequences of SEQ ID NO:4 and SEQ ID NO:6; (e) a set of primers having the nucleotide sequences of SEQ ID NO:4 and SEQ ID NO:7; (f) a set of primers having the nucleotide sequences of SEQ ID NO:7 and SEQ ID NO:8; (g) a set of primers having the nucleotide sequences of SEQ ID NO:9 and SEQ ID NO:10; (h) a set of primers having the nucleotide sequences of SEQ ID NO:11 and SEQ ID NO:12; (i) a set of primers having the nucleotide sequences of SEQ ID NO:13 and SEQ ID NO:14; (j) a set of primers having the nucleotide sequences of SEQ ID NO:15 and SEQ ID NO:16; (k) a set of primers having the nucleotide sequences of SEQ ID NO:17 and SEQ ID NO:18; (l) a set of primers having the nucleotide sequences of SEQ ID NO:19 and SEQ ID NO:20; (m) a set of primers having the nucleotide sequences of SEQ ID NO:21 and SEQ ID NO:22; (n) a set of primers having the nucleotide sequences of SEQ ID NO:23 and SEQ ID NO:24; (o) a set of primers having the nucleotide sequences of SEQ ID NO:25 and SEQ ID NO:26; (p) a set of primers having the nucleotide sequences of SEQ ID NO:27 and SEQ ID NO:28; (q) a set of primers having the nucleotide sequences of SEQ ID NO:29 and SEQ ID NO:30; (r) a set of primers having the nucleotide sequences of SEQ ID NO:31 and SEQ ID NO:32; (s) a set of primers having the nucleotide sequences of SEQ ID NO:33 and SEQ ID NO:34; (t) a set of primers having the nucleotide sequences of SEQ ID NO:35 and SEQ ID NO:36; (u) a set of primers having the nucleotide sequences of SEQ ID NO:37 and SEQ ID NO:38; and (a) a set of primers having the nucleotide sequences of SEQ ID NO:39 and SEQ ID NO:40.

    Abstract translation: 目的:提供用于检测乙型肝炎的PCR引物组和使用相同引物组检测乙型肝炎的方法,从而将检测时间缩短到30分钟,并通过优化PCR条件并使用特异性PCR引物组来提高检测繁殖力 。 构成:用于检测乙型肝炎的PCR引物组选自:(a)一组具有SEQ ID NO:1和SEQ ID NO:2的核苷酸序列的引物; (b)具有SEQ ID NO:1和SEQ ID NO:3的核苷酸序列的一组引物; (c)具有SEQ ID NO:4和SEQ ID NO:5的核苷酸序列的一组引物; (d)具有SEQ ID NO:4和SEQ ID NO:6的核苷酸序列的一组引物; (e)具有SEQ ID NO:4和SEQ ID NO:7的核苷酸序列的一组引物; (f)具有SEQ ID NO:7和SEQ ID NO:8的核苷酸序列的一组引物; (g)具有SEQ ID NO:9和SEQ ID NO:10的核苷酸序列的一组引物; (h)具有SEQ ID NO:11和SEQ ID NO:12的核苷酸序列的一组引物; (i)具有SEQ ID NO:13和SEQ ID NO:14的核苷酸序列的一组引物; (j)具有SEQ ID NO:15和SEQ ID NO:16的核苷酸序列的一组引物; (k)具有SEQ ID NO:17和SEQ ID NO:18的核苷酸序列的一组引物; (1)具有SEQ ID NO:19和SEQ ID NO:20的核苷酸序列的一组引物; (m)具有SEQ ID NO:21和SEQ ID NO:22的核苷酸序列的一组引物; (n)具有SEQ ID NO:23和SEQ ID NO:24的核苷酸序列的一组引物; (o)具有SEQ ID NO:25和SEQ ID NO:26的核苷酸序列的一组引物; (p)一组具有SEQ ID NO:27和SEQ ID NO:28的核苷酸序列的引物; (q)具有SEQ ID NO:29和SEQ ID NO:30的核苷酸序列的一组引物; (r)具有SEQ ID NO:31和SEQ ID NO:32的核苷酸序列的一组引物; 一组具有SEQ ID NO:33和SEQ ID NO:34的核苷酸序列的引物; (t)具有SEQ ID NO:35和SEQ ID NO:36的核苷酸序列的一组引物; (u)具有SEQ ID NO:37和SEQ ID NO:38的核苷酸序列的一组引物; 和(a)具有SEQ ID NO:39和SEQ ID NO:40的核苷酸序列的一组引物。

    교류 전압과 T 채널을 이용하여 제타 포텐셜을 측정하는방법
    74.
    发明公开
    교류 전압과 T 채널을 이용하여 제타 포텐셜을 측정하는방법 失效
    使用替代电压和T通道测量ZETA电位的方法

    公开(公告)号:KR1020040067127A

    公开(公告)日:2004-07-30

    申请号:KR1020030004108

    申请日:2003-01-21

    Abstract: PURPOSE: A method for measuring zeta potential using alternation voltage and T-channel is provided to measure zeta potential between a solid wall and a solvent without track particles. CONSTITUTION: A steady state has come by injecting an electrolytic solution through a first injection port of a T-channel having a grounded output electrode, a first input electrode, and a second input electrode. Direct current is applied to the output electrode from the first and the second input electrodes so that a boundary is formed between the electrolytic solution and a mixed solution of fluorescence. Alternation current is applied to the grounded output electrode from any one of two input electrodes. Zeta potential is calculated by measuring the amplitude of the boundary.

    Abstract translation: 目的:提供使用交变电压和T沟道测量ζ电位的方法,用于测量固体壁和无轨道颗粒的溶剂之间的ζ电位。 构成:通过具有接地输出电极,第一输入电极和第二输入电极的T沟道的第一注入口注入电解液来实现稳定状态。 从第一和第二输入电极向输出电极施加直流电流,使得在电解液和荧光的混合溶液之间形成边界。 交替电流从两个输入电极中的任何一个施加到接地的输出电极。 通过测量边界的振幅来计算ζ电位。

    칩 상의 전기적 미세 검출기
    75.
    发明授权
    칩 상의 전기적 미세 검출기 失效
    칩상의전기적미세검출기

    公开(公告)号:KR100438828B1

    公开(公告)日:2004-07-05

    申请号:KR1020010069502

    申请日:2001-11-08

    CPC classification number: G01N27/44791 G01N27/226 G01N27/4473

    Abstract: A micro-electric detector for use in a micro-analysis system is provided. The micro-electric detector comprises a first substrate having a first surface, comprising at least one microchannel and at least one reservoir in fluid communication with the microchannel; a second substrate having a second surface disposed on the first surface of the first substrate; and a sensing portion comprising at least one pair of first electrodes for detection, which is disposed on the second surface of the second substrate along the microchannel, such that the first electrodes are positioned facing a bottom of the microchannel.

    Abstract translation: 提供了用于微分析系统的微电探测器。 所述微电探测器包括具有第一表面的第一基底,所述第一基底包括至少一个微通道和与所述微通道流体连通的至少一个储存器; 具有设置在所述第一基板的所述第一表面上的第二表面的第二基板; 以及感测部分,所述感测部分包括至少一对用于检测的第一电极,所述感测部分沿着所述微通道设置在所述第二基板的所述第二表面上,使得所述第一电极定位成面向所述微通道的底部。 <图像>

    혼성화된 핵산의 검출감도를 증가시키는 방법
    76.
    发明授权
    혼성화된 핵산의 검출감도를 증가시키는 방법 失效
    혼성화된핵산의검출감도를증가시키는방법

    公开(公告)号:KR100436554B1

    公开(公告)日:2004-06-18

    申请号:KR1020010020573

    申请日:2001-04-17

    Inventor: 조윤경 임희균

    CPC classification number: C12Q1/6834 C12Q2521/307

    Abstract: The present invention provides a method for improving detection sensitivity of hybridized nucleic acid immobilized on a solid support of sensing device for gene assay, by removing non-hybridized nucleic acid probe using nuclease from the solid support. In accordance with the invented method, background signal caused by single stranded probe that is not hybridized with target nucleic acid or signal caused by non-specific binding of target nucleic acid to probe is decreased or removed, which improves detection sensitivity of hybridization with a high accuracy, and minimizes the loss of hybridized nucleic acid in the course of washing background signal removed in the conventional method.

    Abstract translation: 本发明提供了一种通过使用来自固体支持物的核酸酶去除未杂交的核酸探针来提高固定在用于基因测定的感测装置的固体支持物上的杂交核酸的检测灵敏度的方法。 根据本发明的方法,由与靶核酸不杂交的单链探针引起的背景信号或由靶核酸与探针的非特异性结合引起的信号减少或消除,这提高了杂交的检测灵敏度, 准确性,并且使在常规方法中去除背景信号的洗涤过程中杂交核酸的损失最小化。

    프로브의 고정화 검출방법 및 상기 프로브와 표적시료의결합정도 검출방법
    77.
    发明公开
    프로브의 고정화 검출방법 및 상기 프로브와 표적시료의결합정도 검출방법 失效
    用于检测探针的固定化方法和检测探针与目标样品之间的结合度的方法

    公开(公告)号:KR1020030048178A

    公开(公告)日:2003-06-19

    申请号:KR1020010078010

    申请日:2001-12-11

    Abstract: PURPOSE: A method for detecting immobilization of probes and a method for detecting binding degree between the probes and target samples are provided, thereby simultaneously and accurately detecting immobilization of probe DNA and hybridization of probe DNA with target DNA. CONSTITUTION: A method for detecting immobilization of probes comprises the steps of: (1) supplying probes to a biochip with an MOSFET type sensor; (2) combining the probes with the surface of a gate electrode of the MOSFET type sensor; and (3) measuring characteristics of voltage and current passed through the gate electrode. A method for detecting binding degree between probes and target samples comprises the steps of: (1) supplying probes to a biochip with a MOSFET type sensor to combine them with the surface of a gate electrode of the MOSFET type sensor; (2) measuring characteristics of voltage and current passed through the gate electrode; (3) combining target samples with the probes; and (4) measuring characteristics of voltage and current passed through the gate electrode.

    Abstract translation: 目的:提供检测探针固定化的方法和检测探针与靶标结合度的方法,同时准确检测探针DNA的固定和探针DNA与靶DNA的杂交。 构成:用于检测探针固定的方法包括以下步骤:(1)用MOSFET型传感器将探针供应给生物芯片; (2)将探针与MOSFET型传感器的栅电极的表面组合; 和(3)测量通过栅电极的电压和电流的特性。 用于检测探针和目标样品之间的结合度的方法包括以下步骤:(1)向具有MOSFET型传感器的生物芯片供应探针,以将其与MOSFET型传感器的栅电极的表面组合; (2)测量通过栅电极的电压和电流的特性; (3)将目标样品与探针组合; 和(4)测量通过栅电极的电压和电流的特性。

    혼성화된 핵산의 검출감도를 증가시키는 방법
    78.
    发明公开
    혼성화된 핵산의 검출감도를 증가시키는 방법 失效
    用于提高感测装置在检测杂化核酸中的灵敏度的方法

    公开(公告)号:KR1020020080803A

    公开(公告)日:2002-10-26

    申请号:KR1020010020573

    申请日:2001-04-17

    Inventor: 조윤경 임희균

    CPC classification number: C12Q1/6834 C12Q2521/307

    Abstract: PURPOSE: Provided is a method for improving a sensitivity of a sensing device in the detection of a hybridized nucleic acid by removing non-hybridized nucleic acid probes using a nuclease. CONSTITUTION: A sensitivity of a sensing device in the detection of hybridization of nucleic acids is improved by removing a non-hybridized nucleic acid probe, wherein the nucleic acids are fixed onto a base plate which is characteristically made of glass, quartz, silicon or plastic.

    Abstract translation: 目的:提供了通过使用核酸酶去除非杂交核酸探针来提高感测装置在检测杂交核酸中的灵敏度的方法。 构成:通过去除非杂交的核酸探针来改善感测装置在检测核酸杂交中的灵敏度,其中核酸被固定在特征性地由玻璃,石英,硅或塑料制成的基板上 。

    청소기 및 그 제어 방법
    79.
    发明公开
    청소기 및 그 제어 방법 审中-实审
    清洁剂及其控制方法

    公开(公告)号:KR1020170089529A

    公开(公告)日:2017-08-04

    申请号:KR1020160009862

    申请日:2016-01-27

    CPC classification number: A47L5/22 A47L9/28

    Abstract: 청소기는흡입력을생성하는팬모터, 사용자입력을수신하는입력버튼, 외부전원으로부터공급받은교류전력을변환하고, 제1 직류전력을출력하는제1 전원회로, 상기제1 직류전력을공급받아전기에너지를저장하고, 상기저장된전기에너지에의한제2 직류전력을출력하는제2 전원회로, 상기제1 직류전력및 상기제2 직류전력중에적어도하나를공급받아상기팬모터를구동하는구동회로, 상기제2 전원회로로공급되는제1 직류전력을제어하는제1 반도체스위칭회로, 상기구동회로로공급되는상기제1 직류전력및 상기제2 직류전력을제어하는제2 반도체스위칭회로및 상기사용자입력및 상기외부전원의연결상태에따라상기제1 반도체스위치회로및 상기제2 반도체스위치회로를온 또는오프시키는제어신호를출력하는마이크로프로세서를포함할수 있다.

    Abstract translation: 真空吸尘器包括用于产生吸力的风扇电机,用于接收用户输入的输入按钮,用于转换从外部电源供应的AC电力并输出第一DC电力的第一电源电路, 第二电源电路,用于基于存储的电能存储第一DC电力和第二DC电力;驱动电路,用于提供第一DC电力和第二DC电力中的至少一个以驱动风扇电机; 2,用于控制供应到所述电源电路中的第一直流电源的第一半导体开关电路,用于控制第一直流电力并提供给所述驱动电路的第二直流电源和所述用户输入和所述第二半导体开关电路 以及微处理器,用于根据外部电源的连接状态输出用于打开或关闭第一半导体开关电路和第二半导体开关电路的控制信号。

Patent Agency Ranking