Abstract:
본 발명은, 용해하고자 하는 세포 샘플을 준비하는 단계; 세포 샘플의 온도를 높이는 가열 단계; 및 세포 샘플 주위에서 흡열 반응을 일으킴으로써 세포 샘플의 온도를 낮추는 냉각 단계를 포함하는 세포의 용해 방법을 제공한다. 본 발명의 방법에 의하면, 별도의 에너지원이 필요하지 않고 운반이 가능하므로, 장소나 기기에 구애를 받지 않고, 간편하게 세포 용해를 수행할 수 있으며, 특히 시료의 양이 적은 바이오칩 내에서 세포 용해를 행할 경우 그 효과는 더욱 크다 할 수 있다. 또한 세포 용해 효율도, 단순히 가열만을 행한 경우에 비하여, 훨씬 우수한 효율을 나타낸다.
Abstract:
본 발명은 DNA 중합 효소 연쇄 반응(PCR)용 모듈 및 이를 채용한 다중 PCR 시스템에 관한 것으로서, 보다 상세하게는, PCR 반응을 위한 열반응장치와 증폭산물의 감지를 위한 감지장치가 합체된 DNA 중합 효소 연쇄 반응(PCR)용 모듈 및 이를 채용한 다중 PCR 시스템에 관한 것이다.
Abstract:
PURPOSE: A PCR primer set for detection of hepatitis B and a method for detecting hepatitis B using the same primer set are provided, thereby reducing the detection time to 30 minutes and improving the detection reproductivity by optimizing the PCR condition and using specific PCR primer set. CONSTITUTION: The PCR primer set for detection of hepatitis B is selected from the group consisting of: (a) a set of primers having the nucleotide sequences of SEQ ID NO:1 and SEQ ID NO:2; (b) a set of primers having the nucleotide sequences of SEQ ID NO:1 and SEQ ID NO:3; (c) a set of primers having the nucleotide sequences of SEQ ID NO:4 and SEQ ID NO:5; (d) a set of primers having the nucleotide sequences of SEQ ID NO:4 and SEQ ID NO:6; (e) a set of primers having the nucleotide sequences of SEQ ID NO:4 and SEQ ID NO:7; (f) a set of primers having the nucleotide sequences of SEQ ID NO:7 and SEQ ID NO:8; (g) a set of primers having the nucleotide sequences of SEQ ID NO:9 and SEQ ID NO:10; (h) a set of primers having the nucleotide sequences of SEQ ID NO:11 and SEQ ID NO:12; (i) a set of primers having the nucleotide sequences of SEQ ID NO:13 and SEQ ID NO:14; (j) a set of primers having the nucleotide sequences of SEQ ID NO:15 and SEQ ID NO:16; (k) a set of primers having the nucleotide sequences of SEQ ID NO:17 and SEQ ID NO:18; (l) a set of primers having the nucleotide sequences of SEQ ID NO:19 and SEQ ID NO:20; (m) a set of primers having the nucleotide sequences of SEQ ID NO:21 and SEQ ID NO:22; (n) a set of primers having the nucleotide sequences of SEQ ID NO:23 and SEQ ID NO:24; (o) a set of primers having the nucleotide sequences of SEQ ID NO:25 and SEQ ID NO:26; (p) a set of primers having the nucleotide sequences of SEQ ID NO:27 and SEQ ID NO:28; (q) a set of primers having the nucleotide sequences of SEQ ID NO:29 and SEQ ID NO:30; (r) a set of primers having the nucleotide sequences of SEQ ID NO:31 and SEQ ID NO:32; (s) a set of primers having the nucleotide sequences of SEQ ID NO:33 and SEQ ID NO:34; (t) a set of primers having the nucleotide sequences of SEQ ID NO:35 and SEQ ID NO:36; (u) a set of primers having the nucleotide sequences of SEQ ID NO:37 and SEQ ID NO:38; and (a) a set of primers having the nucleotide sequences of SEQ ID NO:39 and SEQ ID NO:40.
Abstract translation:目的:提供用于检测乙型肝炎的PCR引物组和使用相同引物组检测乙型肝炎的方法,从而将检测时间缩短到30分钟,并通过优化PCR条件并使用特异性PCR引物组来提高检测繁殖力 。 构成:用于检测乙型肝炎的PCR引物组选自:(a)一组具有SEQ ID NO:1和SEQ ID NO:2的核苷酸序列的引物; (b)具有SEQ ID NO:1和SEQ ID NO:3的核苷酸序列的一组引物; (c)具有SEQ ID NO:4和SEQ ID NO:5的核苷酸序列的一组引物; (d)具有SEQ ID NO:4和SEQ ID NO:6的核苷酸序列的一组引物; (e)具有SEQ ID NO:4和SEQ ID NO:7的核苷酸序列的一组引物; (f)具有SEQ ID NO:7和SEQ ID NO:8的核苷酸序列的一组引物; (g)具有SEQ ID NO:9和SEQ ID NO:10的核苷酸序列的一组引物; (h)具有SEQ ID NO:11和SEQ ID NO:12的核苷酸序列的一组引物; (i)具有SEQ ID NO:13和SEQ ID NO:14的核苷酸序列的一组引物; (j)具有SEQ ID NO:15和SEQ ID NO:16的核苷酸序列的一组引物; (k)具有SEQ ID NO:17和SEQ ID NO:18的核苷酸序列的一组引物; (1)具有SEQ ID NO:19和SEQ ID NO:20的核苷酸序列的一组引物; (m)具有SEQ ID NO:21和SEQ ID NO:22的核苷酸序列的一组引物; (n)具有SEQ ID NO:23和SEQ ID NO:24的核苷酸序列的一组引物; (o)具有SEQ ID NO:25和SEQ ID NO:26的核苷酸序列的一组引物; (p)一组具有SEQ ID NO:27和SEQ ID NO:28的核苷酸序列的引物; (q)具有SEQ ID NO:29和SEQ ID NO:30的核苷酸序列的一组引物; (r)具有SEQ ID NO:31和SEQ ID NO:32的核苷酸序列的一组引物; 一组具有SEQ ID NO:33和SEQ ID NO:34的核苷酸序列的引物; (t)具有SEQ ID NO:35和SEQ ID NO:36的核苷酸序列的一组引物; (u)具有SEQ ID NO:37和SEQ ID NO:38的核苷酸序列的一组引物; 和(a)具有SEQ ID NO:39和SEQ ID NO:40的核苷酸序列的一组引物。
Abstract:
PURPOSE: A method for measuring zeta potential using alternation voltage and T-channel is provided to measure zeta potential between a solid wall and a solvent without track particles. CONSTITUTION: A steady state has come by injecting an electrolytic solution through a first injection port of a T-channel having a grounded output electrode, a first input electrode, and a second input electrode. Direct current is applied to the output electrode from the first and the second input electrodes so that a boundary is formed between the electrolytic solution and a mixed solution of fluorescence. Alternation current is applied to the grounded output electrode from any one of two input electrodes. Zeta potential is calculated by measuring the amplitude of the boundary.
Abstract:
A micro-electric detector for use in a micro-analysis system is provided. The micro-electric detector comprises a first substrate having a first surface, comprising at least one microchannel and at least one reservoir in fluid communication with the microchannel; a second substrate having a second surface disposed on the first surface of the first substrate; and a sensing portion comprising at least one pair of first electrodes for detection, which is disposed on the second surface of the second substrate along the microchannel, such that the first electrodes are positioned facing a bottom of the microchannel.
Abstract:
The present invention provides a method for improving detection sensitivity of hybridized nucleic acid immobilized on a solid support of sensing device for gene assay, by removing non-hybridized nucleic acid probe using nuclease from the solid support. In accordance with the invented method, background signal caused by single stranded probe that is not hybridized with target nucleic acid or signal caused by non-specific binding of target nucleic acid to probe is decreased or removed, which improves detection sensitivity of hybridization with a high accuracy, and minimizes the loss of hybridized nucleic acid in the course of washing background signal removed in the conventional method.
Abstract:
PURPOSE: A method for detecting immobilization of probes and a method for detecting binding degree between the probes and target samples are provided, thereby simultaneously and accurately detecting immobilization of probe DNA and hybridization of probe DNA with target DNA. CONSTITUTION: A method for detecting immobilization of probes comprises the steps of: (1) supplying probes to a biochip with an MOSFET type sensor; (2) combining the probes with the surface of a gate electrode of the MOSFET type sensor; and (3) measuring characteristics of voltage and current passed through the gate electrode. A method for detecting binding degree between probes and target samples comprises the steps of: (1) supplying probes to a biochip with a MOSFET type sensor to combine them with the surface of a gate electrode of the MOSFET type sensor; (2) measuring characteristics of voltage and current passed through the gate electrode; (3) combining target samples with the probes; and (4) measuring characteristics of voltage and current passed through the gate electrode.
Abstract:
PURPOSE: Provided is a method for improving a sensitivity of a sensing device in the detection of a hybridized nucleic acid by removing non-hybridized nucleic acid probes using a nuclease. CONSTITUTION: A sensitivity of a sensing device in the detection of hybridization of nucleic acids is improved by removing a non-hybridized nucleic acid probe, wherein the nucleic acids are fixed onto a base plate which is characteristically made of glass, quartz, silicon or plastic.
Abstract:
본 발명에 의한 진공청소기의 집진장치는 오물분리부; 상기 오물분리부에서 여과된 오물이 포집되는 오물수거통; 및 상기 오물분리부와 오물수거통 사이에 개재되어, 상기 오물수거통에 포집된 오물을 압축하는 먼지압축장치;를 포함하는 것을 특징으로 한다. 이상과 같은 본 발명에 의하면, 한정된 집진장치 내부의 부피에 포집되는 먼지를 압축하여 수용할 수 있어, 사용자가 집진장치를 자주 청소할 필요가 없어 편리하다. 진공청소기, 집진장치, 사이클론, 먼지압축, 압축