公开(公告)号：IN387CH2008A

公开(公告)日：2008-09-19

申请号：IN387CH2008

申请日：2008-01-23

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  KIEFER PATRICK ,  HAEFNER STEFAN ,  KROEGER BURKHARD ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH

IPC: C12N9/16 ,  C12P13/08

Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i.e., fructose-1,6-bisphosphatase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of fructose-1,6-bisphosphatase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

公开(公告)号：IN2603CH2006A

公开(公告)日：2007-06-08

申请号：IN2603CH2006

申请日：2004-12-17

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  KIEFER PATRICK ,  HAEFNER STEFAN ,  KROEGER BURKHARD ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH

IPC: C12N9/16 ,  C12P13/08

Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i.e., fructose-1,6-bisphosphatase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of fructose-1,6-bisphosphatase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

公开(公告)号：IN2600CH2006A

公开(公告)日：2007-06-08

申请号：IN2600CH2006

申请日：2006-07-17

Applicant: BASF AG

Inventor： GER BURKHARD KR ,  ZELDER OSKAR ,  KLOPPROGGER CORINNA ,  DER HARTWIG SCHR ,  HAEFNER STEFAN

IPC: C07K14/34 ,  C12N15/77 ,  C12P13/08

Abstract: Use of a nucleic acid (I) with promoter activity for transcribing genes, where (I) is (a) a 164 bp sequence (SEQ ID No,:1); (b) a variant of (SEQ ID No.:1) with at least 90% identity and derived by substitution, insertion or deletion of nucleotides; (c) a sequence that hybridizes to (SEQ ID No.:1) under stringent conditions; or (d) a functionally equivalent fragment of (a)-(c), is new. Independent claims are also included for : (1) use of an expression unit (EU), containing (I) and functionally linked to a sequence (X) that ensures translation of RNA, for expressing genes; (2) (I), other than sequence (1), i.e. (Ia), as new compounds; (3) EU that contain (Ia) linked to (X); (4) altering (or producing) the transcription rate of genes in a microorganism, relative to the wild type; (5) expression cassette (EC) comprising at least one EU of (1), at least one other nucleic acid sequence (to be expressed) and optionally additional gene control elements, where at least the first two are linked and the sequence being expressed is heterologous with respect to EU; (6) expression vector (EV) that contains EC; (7) genetically modified microorganisms (GMO) having, for at least one gene, an altered (or induced) transcription rate, relative to the wild type; (8) preparing biosynthetic products by culturing GMO of (7); (9) use of the sequence ggaggga (53) as a ribosome-binding site in expression units that provide expression of genes in Corynebacterium or Brevibacterium; (10) use of the sequence tagagt (52) as a -10 region for expression of genes in Corynebacterium or Brevibacterium; and (11) expression units that contain sequences (52) or (53).

公开(公告)号：BRPI0417772A

公开(公告)日：2007-04-17

申请号：BRPI0417772

申请日：2004-12-17

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  KROEGER BURKHARD ,  KIEFER PATRICK ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH

IPC: C12N15/52 ,  C12N9/16 ,  C12P13/08

Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i.e., fructose-1,6-bisphosphatase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of fructose-1,6-bisphosphatase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

公开(公告)号：BRPI0417770A

公开(公告)日：2007-04-17

申请号：BRPI0417770

申请日：2004-12-15

Applicant: BASF AG

Inventor： GER BURKHARD KR ,  ZELDER OSKAR ,  KLOPPROGGER CORINNA ,  DER HARTWIG SCHR ,  HAEFNER STEFAN

IPC: C12N15/77 ,  C07K14/34 ,  C12P13/08

Abstract: Use of a nucleic acid (I) with promoter activity for transcribing genes, where (I) is (a) a 164 bp sequence (SEQ ID No,:1); (b) a variant of (SEQ ID No.:1) with at least 90% identity and derived by substitution, insertion or deletion of nucleotides; (c) a sequence that hybridizes to (SEQ ID No.:1) under stringent conditions; or (d) a functionally equivalent fragment of (a)-(c), is new. Independent claims are also included for : (1) use of an expression unit (EU), containing (I) and functionally linked to a sequence (X) that ensures translation of RNA, for expressing genes; (2) (I), other than sequence (1), i.e. (Ia), as new compounds; (3) EU that contain (Ia) linked to (X); (4) altering (or producing) the transcription rate of genes in a microorganism, relative to the wild type; (5) expression cassette (EC) comprising at least one EU of (1), at least one other nucleic acid sequence (to be expressed) and optionally additional gene control elements, where at least the first two are linked and the sequence being expressed is heterologous with respect to EU; (6) expression vector (EV) that contains EC; (7) genetically modified microorganisms (GMO) having, for at least one gene, an altered (or induced) transcription rate, relative to the wild type; (8) preparing biosynthetic products by culturing GMO of (7); (9) use of the sequence ggaggga (53) as a ribosome-binding site in expression units that provide expression of genes in Corynebacterium or Brevibacterium; (10) use of the sequence tagagt (52) as a -10 region for expression of genes in Corynebacterium or Brevibacterium; and (11) expression units that contain sequences (52) or (53).

公开(公告)号：BRPI0417726A

公开(公告)日：2007-04-03

申请号：BRPI0417726

申请日：2004-12-16

Applicant: BASF AG

Inventor： KLOPPROGGE CORINNA ,  ZELDER OSKAR ,  GER BURKHARD KR ,  DER HARTWIG SCHR ,  HAEFNER STEFAN ,  RUFFER UWE ,  GRAEF CLAUDIA ISABELLA ,  HABERHAUER GREGOR

IPC: C07K14/195 ,  C07K14/34

Abstract: The present invention relates to mutated nucleic acids and proteins of the metabolic pathway of fine chemicals, to processes for preparing genetically modified producer organisms, to processes for preparing fine chemicals by culturing genetically modified organisms, and to genetically modified organisms themselves.

公开(公告)号：BRPI0417706A

公开(公告)日：2007-03-20

申请号：BRPI0417706

申请日：2004-12-17

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  KRUEGER BURKHARD ,  KIEFER PATRICK ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH

IPC: C12P20060101 ,  C12P13/08 ,  C12N9/12 ,  C12N15/54 ,  C12N1/21

Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i. e., glycerol kinase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of glycerol kinase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

公开(公告)号：MXPA06006859A

公开(公告)日：2007-01-26

申请号：MXPA06006859

申请日：2004-12-17

Applicant: BASF AG

Inventor： SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  HAEFNER STEFAN ,  KIEFER PATRICK ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH

IPC: C12N1/00 ,  C12P13/08 ,  C12N9/00 ,  C12N15/00 ,  C12P20060101

Abstract: La presente invencion muestra metodos para aumentar la produccion de un quimico fino, por ejemplo, lisina a partir de un microorganismo, por ejemplo, Corynebacterium al desregularizar un gen que codifica la enzima, es decir, glicerol kinasa. En una modalidad preferida, la invencion provee metodos para aumentar la produccion de lisina en Corynebacterium glutamicum al aumentar la expresion de la actividad de glicerol kinasa. La invencion tambien provee un proceso novedoso para la produccion de lisina al regular el flujo de carbono hacia oxaloacetato (OAA). En una modalidad preferida, la invencion provee metodos para la produccion de lisina al utilizar fructosa o sacarosa como una fuente de carbono.

公开(公告)号：MXPA06006758A

公开(公告)日：2007-01-26

申请号：MXPA06006758

申请日：2004-12-17

Applicant: BASF AG

Inventor： SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  HAEFNER STEFAN ,  KIEFER PATRICK ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH

IPC: C12N15/00 ,  C12P13/08 ,  C12N1/00 ,  C12N9/00 ,  C12N9/04 ,  C12R1/15

Abstract: La presente invencion presenta metodos para incrementar la produccion de un quimico fino, por ejemplo, lisina, a partir de un microorganismo, por ejemplo, Corynebacterium mediante la desregulacion de un gen que codifica una enzima, lactato deshidrogenasa. En una modalidad, la invencion ofrece metodos para incrementar la produccion de lisina en Corynebacterium glutamicum mediante el incremento de la expresion de la actividad lactato deshidrogenasa. La invencion ofrece tambien un proceso novedoso para la produccion de lisina mediante la regulacion del flujo de carbono hacia oxaloacetato (OAA). En una modalidad preferida, la invencion ofrece metodos para la produccion de lisina mediante la utilizacion de fructosa o sucrosa como fuente de carbono.

公开(公告)号：CA2615416A1

公开(公告)日：2007-01-25

申请号：CA2615416

申请日：2006-07-18

Applicant: BASF AG

Inventor： SCHRODER HARTWIG ,  YOCUM ROGERS R ,  HAEFNER STEFAN ,  HERMANN THERON ,  ZELDER OSKAR ,  PATTERSON THOMAS A ,  PERO JANICE G ,  HEROLD ANDREA ,  WILLIAMS MARK K ,  KLOPPROGGE CORINNA

IPC: C12P13/12

Abstract: This invention relates to methionine producing recombinant microorganisms. Specifically, this invention relates to recombinant strains of Corynebacterium that produce increased levels of methionine compared to their wild-type counterparts and further to methods of generating such microorganisms.