公开(公告)号：DK591684D0

公开(公告)日：1984-12-11

申请号：DK591684

申请日：1984-12-11

Applicant: MILES LAB

Inventor： ALBARELLA JAMES P ,  ANDERSON LESLIE H ,  CARRICO ROBERT J

IPC: C07K16/18 ,  C12Q1/68 ,  C12Q1/70 ,  G01N ,  C12Q

公开(公告)号：CA1133474A

公开(公告)日：1982-10-12

申请号：CA320897

申请日：1979-02-06

Applicant: MILES LAB

Inventor： BOGUSLASKI ROBERT C ,  CARRICO ROBERT J ,  BURD JOHN F

IPC: C07D209/48 ,  C07D233/78 ,  C07H17/075 ,  C07H21/00 ,  G01N33/533 ,  G01N33/535 ,  C07H17/06

Abstract: An improved specific binding assay method and reagent for determining a ligand in a liquid medium employing, as an enzyme-cleavable substrate label, a residue having the formula: G-D-R wherein G is a glycone, D is a dye indicator moiety, and R is a linking group through which the label residue is covalently bound to a binding component of a conventional binding assay system, such as the ligand, an analog thereof, or a specific binding partner thereof. The monitored characteristic of the label is the release of a detectable product, usually a fluorogen or chromogen, upon enzymatic cleavage of the glycosidic linkage between the glycone and the dye indicator moiety. The assay method may follow a homogeneous or heterogeneous format. The preferred glycone is a .beta.-galactosyl group and the preferred dye indicator moiety is an umbelliferone residue. The improved assay is particularly suited to the determination of haptens such as drugs, particularly the aminoglycoside antibiotics.

公开(公告)号：CA1078712A

公开(公告)日：1980-06-03

申请号：CA249744

申请日：1976-04-07

Applicant: MILES LAB

Inventor： BOGUSIASKI ROBERT C ,  CARRICO ROBERT J ,  CHRISTNER JAMES E

IPC: G01N33/542 ,  C07D209/48 ,  C07D237/32 ,  C07D493/10 ,  C07D495/04 ,  C07H19/20 ,  C07H19/207 ,  C07H21/00 ,  C12Q1/00 ,  G01N31/00 ,  G01N33/50 ,  G01N33/532 ,  G01N33/533 ,  G01N33/536 ,  G01N33/543 ,  G01N33/60 ,  G01N33/16

Abstract: An improved heterogenous specific binding assay method which employs a substance having reactant activity, i.e., a reactant, as a labeling substance in the detection of a ligand in a liquid medium. The method is carried out using reagent means which comprises, as its labeled constituent, a conjugate formed of a specific binding substance coupled to the reactant. The reactant advantageously is an enzymatic reactant such as an enzyme substrate or coenzyme. The activity of the conjugated reactant as a constituent of a predetermined reaction system is utilized as means for monitoring the extent of binding of the labeled constituent in conventional heterogenous specific binding assay schemes. The presence of a ligand in a liquid medium may be determined following conventional competitive binding manipulative techniques. After the necessary separation of the boundand free-phases resulting in the specific binding reaction system, the extent of binding of the labeled constituent is determined by contacting either phase with the necessary materials to form the predetermined monitoring reaction system in which the labeling substance is active and assessing reactant activity therein.