公开(公告)号：KR100516377B1

公开(公告)日：2005-09-26

申请号：KR1020030023164

申请日：2003-04-12

Applicant: (주)케비젠

Inventor： 김소미 ,  오덕근 ,  백대헌 ,  신홍식 ,  박시호 ,  이유진 ,  엄수종 ,  노영쇠 ,  박종섭

IPC: C12N1/20

CPC classification number: C12P7/6427 ,  A23C9/1234 ,  A23C11/106 ,  A23L33/12 ,  A23L33/135 ,  A23P10/30 ,  A23Y2300/59 ,  A61K35/744 ,  A61K35/745 ,  C12R1/13

Abstract: 본 발명은 공역화 리놀레인산(CLA)을 생산할 수 있는 신규한 균주에 관한 것이다.
본 발명의 균주에는 비피도박테리움 브레베(
Bifidobacterium breve ) CBG-C2 균주, 비피도박테리움 슈도카르테눌라툼(
Bifidobacterium pseudocartenulatum ) CBG-C4 균주 및 엔테로코커스 패시움(
Enterococcus faecium ) CBG-C5 균주가 포함된다.
본 발명의 균주는 CLA 생산 능력이 우수하며, CLA를 생성하여 배지로 분비하는 한편 균체 내에 축적할 수 있다. 또한 본 발명의 균주는 위산이나 담즙 등의 산과 항생물질에 대해 강한 내성을 지닌다.
본 발명의 균주를 포함하는 조성물은 수용성 다당류로 이루어진 피복물질 내에 본 발명의 균주와 CLA를 포함하는 캡슐제 형태로 제조되어, 기능성 식품 및 의약품으로 사용될 수 있다.

公开(公告)号：KR100464061B1

公开(公告)日：2004-12-30

申请号：KR1020020026615

申请日：2002-05-15

Applicant: (주)케비젠

Inventor： 오덕근 ,  김소미 ,  신홍식 ,  엄수종 ,  노영쇠 ,  박종섭 ,  김동명 ,  박시호

IPC: C12P19/02

Abstract: PURPOSE: A method for producing tagatose by immobilization of arabinose isomerase is provided. Tagatose is cheaply and effectively mass-produced by maintaining the enzyme activity for a long time. CONSTITUTION: A method for producing tagatose by immobilization of arabinose isomerase comprises binding arabinose isomerase to resin, bridging arabinose isomerase with glutaraldehyde to immobilize the arabinose isomerase, and reacting the immobilized arabinose isomerase with substrate, wherein the concentration of arabinose isomerase is 10 mg/ml; the resin is sodium alginate, alumina or silica; the sodium alginate is used by dissolving 1.5 to 4.0% of sodium alginate in water; the alumina is used by dissolving 60 to 70% of alumina in 50 mM tris buffer solution; the silica is used by dissolving 60 to 70% of silica in 50 mM tris buffer solution; substrate is galactose; the galactose is used by dissolving 45 to 55% of galactose in pH 7 to 8 tris buffer solution; and the reaction temperature is 55 to 65 deg. C.

Abstract translation: 目的：提供一种通过固定阿拉伯糖异构酶来生产塔格糖的方法。 塔格糖通过长期维持酶活性而廉价且有效地大量生产。 构成：通过固定阿拉伯糖异构酶生产塔格糖的方法包括将阿拉伯糖异构酶与树脂结合，将阿拉伯糖异构酶与戊二醛桥接以固定阿拉伯糖异构酶，并使固定的阿拉伯糖异构酶与底物反应，其中阿拉伯糖异构酶的浓度为10mg / ml ; 该树脂是海藻酸钠，氧化铝或二氧化硅; 海藻酸钠通过在水中溶解1.5至4.0％的海藻酸钠来使用; 通过在50mM tris缓冲溶液中溶解60-70％的氧化铝来使用氧化铝; 二氧化硅是通过将60-70％的二氧化硅溶解在50mM tris缓冲溶液中而使用的; 底物是半乳糖; 半乳糖通过在pH 7至8 Tris缓冲溶液中溶解45-55％半乳糖来使用; 反应温度为55〜65℃。 C。

公开(公告)号：KR100437102B1

公开(公告)日：2004-06-23

申请号：KR1020020001178

申请日：2002-01-09

Applicant: (주)케비젠

Inventor： 신홍식 ,  박시호 ,  노영쇠 ,  엄수종 ,  권윤자 ,  박명순 ,  한혜숙 ,  정민숙 ,  김소미 ,  김동명 ,  오덕근 ,  박종섭

IPC: C07K5/00

Abstract: PURPOSE: Provided are a novel retinol derivative, its preparation method in higher yield, and its use. The novel retinol has excellent light-stability, and shows high reactivity to retinoic acid receptor α, while showing low reactivity to retinoic receptor β and γ. It can applied to medical products, cosmetics, soap, shampoo, functional foods, etc., for the prevention and improvement of skin aging. CONSTITUTION: The novel retinol derivative is characterized by carboester bond of a peptide material having COOH group, wherein the peptide material having COOH group is selected from -di, -tri, -poly peptide including N-L- α-aspartyl-L-phenylalanine 1-methylester(AMP;aspartame), N-protection group-aspartame, neotame and the like. Its manufacturing method comprises the steps of: reacting retinylacetate with methanolic solvent and inorganic slat at 25-40 deg.C in a dark room then extracting the reaction product with ether solvent; removing the solvent then followed by mixing a compound having OH group, natural or separated and purified retinol, diethylazodicarboxylate and triphenylphosphate with methylenechloride solvent, and reacting them at room temperature to obtain the ester derivative of retinol; and performing chromatography with reverse-phase, Merck Silicagel 60 RP 18(40-63) micro meter to separate pure ester derivative of retinol.

Abstract translation: 目的：提供一种新颖的视黄醇衍生物及其制备方法，其收率较高，并且其用途。 该新型视黄醇具有优异的光稳定性，并且对视黄酸受体α具有高反应性，而对视黄酸受体β具有低反应性; 和＆amp; gamma ;. 它可以应用于医疗产品，化妆品，肥皂，洗发水，功能性食品等，以预防和改善皮肤老化。 构成：新颖的视黄醇衍生物的特征在于具有COOH基团的肽材料的羧基酯键，其中具有COOH基团的肽材料选自包括NL-和α-天冬氨酰-L- 苯丙氨酸1-甲酯（AMP;天冬甜素），N-保护基 - 阿斯巴甜，纽甜等。 其制造方法包括以下步骤：在25-40℃，在黑暗的房间内将乙酸视黄醇酯与甲醇溶剂和无机薄片反应，然后用乙醚溶剂萃取反应产物; 然后除去溶剂，然后将具有OH基团的化合物，天然或分离纯化的视黄醇，偶氮二羧酸二乙酯和磷酸三苯酯与二氯甲烷溶剂混合，并在室温下反应，得到视黄醇的酯衍生物; 并用反相色谱（Merck Silicagel 60 RP 18（40-63）微米）进行色谱分离以分离视黄醇的纯酯衍生物。

公开(公告)号：KR1020040020426A

公开(公告)日：2004-03-09

申请号：KR1020020052026

申请日：2002-08-30

Applicant: (주)케비젠

Inventor： 신홍식 ,  엄수종 ,  권윤자 ,  한혜숙 ,  박시호 ,  박명순 ,  백대헌

IPC: C07C403/20

Abstract: PURPOSE: Retinoid derivatives in cis form having substantially different chemical structure while having increased antitumor effects but reduced negative side effects compared with the conventional retinoid compounds, are provided. CONSTITUTION: The retinoid derivatives are represented by formula (I) and can be in cis form especially at the position of 9 and/or 13, wherein X is O, NH or S; R1 and R2 are the same or different, and independently -OH, -SH, -NH2, -COOH, -R(CH2)mCH3, -RCOCO(CH2)mCH3, -RCO(CH2)mCHCH3CH3, -RCO(CH2)mNR4CH3, -RCOCHOH(CH2)mCH3, -RCOCH2(CH2)m(CH)3, -RCOCH2CHOH(CH2)mCH3, -RCOCH2(CH2)mCOOH, -RSO2CH2(CH2)mCH4, -RPO2(OH)CH2(CH2)mCH3 or -RCOCH(NHCOCH3)CH2CH2CONH2; and R3 is H, wherein R is CH2, O, NH or S, R4 is H or C1-C6 alkyl, and m is an integer of 0 to 5.

Abstract translation: 目的：提供具有与常规类视黄醇化合物相比具有显着不同化学结构同时具有增加的抗肿瘤作用但降低的副作用的顺式形式的类视黄醇衍生物。 构成：类视黄醇衍生物由式（I）表示，并且可以是顺式，特别是在9和/或13位，其中X是O，NH或S; R1和R2相同或不同，独立地是-OH，-SH，-NH2，-COOH，-R（CH2）mCH3，-RCOCO（CH2）mCH3，-RCO（CH2）mCHCH3CH3，-RCO（CH2）mNR4CH3 ，-RCOCHOH（CH2）mCH3，-RCOCH2（CH2）m（CH）3，-RCOCH2CHOH（CH2）mCH3，-RCOCH2（CH2）mCOOH，-RSO2CH2（CH2）mCH4，-RPO2（OH）CH2（CH2） 或-RCOCH（NHCOCH 3）CH 2 CH 2 CONH 2; 并且R 3是H，其中R是CH 2，O，NH或S，R 4是H或C 1 -C 6烷基，m是0-5的整数。

公开(公告)号：KR1020030088749A

公开(公告)日：2003-11-20

申请号：KR1020020026615

申请日：2002-05-15

Applicant: (주)케비젠

Inventor： 오덕근 ,  김소미 ,  신홍식 ,  엄수종 ,  노영쇠 ,  박종섭 ,  김동명 ,  박시호

IPC: C12P19/02

Abstract: PURPOSE: A method for producing tagatose by immobilization of arabinose isomerase is provided. Tagatose is cheaply and effectively mass-produced by maintaining the enzyme activity for a long time. CONSTITUTION: A method for producing tagatose by immobilization of arabinose isomerase comprises binding arabinose isomerase to resin, bridging arabinose isomerase with glutaraldehyde to immobilize the arabinose isomerase, and reacting the immobilized arabinose isomerase with substrate, wherein the concentration of arabinose isomerase is 10 mg/ml; the resin is sodium alginate, alumina or silica; the sodium alginate is used by dissolving 1.5 to 4.0% of sodium alginate in water; the alumina is used by dissolving 60 to 70% of alumina in 50 mM tris buffer solution; the silica is used by dissolving 60 to 70% of silica in 50 mM tris buffer solution; substrate is galactose; the galactose is used by dissolving 45 to 55% of galactose in pH 7 to 8 tris buffer solution; and the reaction temperature is 55 to 65 deg. C.

Abstract translation: 目的：提供通过阿拉伯糖异构酶固定生产塔格糖的方法。 通过长时间维持酶活性，塔提糖便宜而有效地批量生产。 构成：通过阿拉伯糖异构酶的固定制备塔格糖的方法包括将阿拉伯糖异构酶与树脂结合，用戊二醛桥接阿拉伯糖异构酶以固定阿拉伯糖异构酶，并使固定的阿拉伯糖异构酶与底物反应，其中阿拉伯糖异构酶的浓度为10mg / ml ; 树脂是藻酸钠，氧化铝或二氧化硅; 通过将1.5〜4.0％的藻酸钠溶于水中使用藻酸钠; 通过将60〜70％的氧化铝溶解在50mM tris缓冲溶液中来使用氧化铝; 通过将60〜70％的二氧化硅溶解在50mM tris缓冲溶液中来使用二氧化硅; 底物是半乳糖; 通过将45至55％的半乳糖溶解在pH7至8 tris缓冲溶液中来使用半乳糖; 反应温度为55〜65℃。 C。

公开(公告)号：KR1020030075213A

公开(公告)日：2003-09-26

申请号：KR1020020014263

申请日：2002-03-16

Applicant: (주)케비젠

Inventor： 김동명 ,  박종섭 ,  김소미 ,  노영쇠 ,  박시호 ,  신홍식 ,  엄수종 ,  오덕근

IPC: A61K9/50

CPC classification number: A61K9/7007 ,  A61K9/5021

Abstract: PURPOSE: A process of preparing a water-soluble film having a high solid content, a low viscosity at wide pH range and excellent dispersion stability without causing solidification is provided. Therefore, the film has excellent dispersion stability and prevents the anticancer substances from being released fast after the destruction of the resin film and is thus effectively used in controlled released drug delivery systems, skin absorbing agents, patch agents or the like. CONSTITUTION: A water-soluble film(1) is prepared by: forming a microcapsule by coating anticancer substances(3) with aminoaldehyde polycondensated resin; and then coating the microcapsule with a water-soluble polymer.

Abstract translation: 目的：提供固体成分高，在宽pH范围内的低粘度，优异的分散稳定性而不引起凝固的水溶性薄膜的制造方法。 因此，该膜具有优异的分散稳定性，并且防止了抗癌物质在树脂膜破坏之后快速释放，因此有效地用于受控释放的药物递送系统，皮肤吸收剂，贴剂等中。 构成：通过以下方法制备水溶性薄膜（1）：通过用氨基醛缩聚树脂涂覆抗癌物质（3）形成微胶囊; 然后用水溶性聚合物涂布微胶囊。

公开(公告)号：KR1020020060598A

公开(公告)日：2002-07-18

申请号：KR1020020001178

申请日：2002-01-09

Applicant: (주)케비젠

Inventor： 신홍식 ,  박시호 ,  노영쇠 ,  엄수종 ,  권윤자 ,  박명순 ,  한혜숙 ,  정민숙 ,  김소미 ,  김동명 ,  오덕근 ,  박종섭

IPC: C07K5/00

CPC classification number: C07C403/08 ,  A23L33/155 ,  A61K8/671 ,  A61K31/07 ,  A61Q5/02 ,  A61Q19/005 ,  A61Q19/008 ,  A61Q19/10 ,  C07K5/04 ,  C07K19/00

Abstract: PURPOSE: Provided are a novel retinol derivative, its preparation method in higher yield, and its use. The novel retinol has excellent light-stability, and shows high reactivity to retinoic acid receptor α, while showing low reactivity to retinoic receptor β and γ. It can applied to medical products, cosmetics, soap, shampoo, functional foods, etc., for the prevention and improvement of skin aging. CONSTITUTION: The novel retinol derivative is characterized by carboester bond of a peptide material having COOH group, wherein the peptide material having COOH group is selected from -di, -tri, -poly peptide including N-L- α-aspartyl-L-phenylalanine 1-methylester(AMP;aspartame), N-protection group-aspartame, neotame and the like. Its manufacturing method comprises the steps of: reacting retinylacetate with methanolic solvent and inorganic slat at 25-40 deg.C in a dark room then extracting the reaction product with ether solvent; removing the solvent then followed by mixing a compound having OH group, natural or separated and purified retinol, diethylazodicarboxylate and triphenylphosphate with methylenechloride solvent, and reacting them at room temperature to obtain the ester derivative of retinol; and performing chromatography with reverse-phase, Merck Silicagel 60 RP 18(40-63) micro meter to separate pure ester derivative of retinol.

Abstract translation: 目的：提供一种新型视黄醇衍生物，其制备方法较高产率及其用途。 该新型视黄醇具有优异的光稳定性，对视黄酸受体α具有高反应性，而对视黄酸受体β和γ的反应性低。 适用于医疗用品，化妆品，肥皂，洗发水，功能性食品等，用于预防和改善皮肤老化。 构成：新型视黄醇衍生物的特征在于具有COOH基团的肽材料的碳键，其中具有COOH基团的肽材料选自-di，-tri， - 多肽，包括NL-α-天冬氨酰-L-苯丙氨酸1- 甲基酯（AMP;阿斯巴甜），N-保护基 - 阿斯巴甜，neotame等。 其制造方法包括以下步骤：将黑醋酸乙烯酯与甲醇溶剂和无机条板在25-40℃下在暗室中反应，然后用乙醚溶剂萃取反应产物; 除去溶剂，然后将具有OH基团的化合物，天然或分离的纯化的视黄醇，偶氮二甲酸二乙酯和磷酸三苯酯与二氯甲烷溶剂混合，并在室温下使其反应，得到视黄醇的酯衍生物; 并用反相色谱法，Merck Silicagel 60 RP 18（40-63）微米分离纯化的视黄醇酯衍生物。