公开(公告)号：KR100514593B1

公开(公告)日：2005-09-13

申请号：KR1020050004128

申请日：2005-01-17

Applicant: (주)케비젠

Inventor： 김소미 ,  오덕근 ,  백대헌 ,  신홍식 ,  박시호 ,  이유진 ,  엄수종 ,  노영쇠 ,  박종섭

IPC: C12N1/20

CPC classification number: C12P7/6427 ,  A23C9/1234 ,  A23C11/106 ,  A23L33/12 ,  A23L33/135 ,  A23P10/30 ,  A23Y2300/59 ,  A61K35/744 ,  A61K35/745 ,  C12R1/13

Abstract: 본 발명은 공역화 리놀레인산(CLA)을 생산할 수 있는 신규한 균주에 관한 것이다.
본 발명의 균주에는 비피도박테리움 브레베(
Bifidobacterium breve ) CBG-C2 균주, 비피도박테리움 슈도카르테눌라툼(
Bifidobacterium pseudocartenulatum ) CBG-C4 균주 및 엔테로코커스 패시움(
Enterococcus faecium ) CBG-C5 균주가 포함된다.
본 발명의 균주는 CLA 생산 능력이 우수하며, CLA를 생성하여 배지로 분비하는 한편 균체 내에 축적할 수 있다. 또한 본 발명의 균주는 위산이나 담즙 등의 산과 항생물질에 대해 강한 내성을 지닌다.
본 발명의 균주를 포함하는 조성물은 수용성 다당류로 이루어진 피복물질 내에 본 발명의 균주와 CLA를 포함하는 캡슐제 형태로 제조되어, 기능성 식품 및 의약품으로 사용될 수 있다.

公开(公告)号：KR1020030089396A

公开(公告)日：2003-11-21

申请号：KR1020020066071

申请日：2002-10-29

Applicant: (주)케비젠

Inventor： 엄수종 ,  신홍식 ,  박시호 ,  백대헌 ,  전국체 ,  박명순 ,  박종섭 ,  노영쇠 ,  오덕근

IPC: A61K36/488 ,  A61P35/00

Abstract: PURPOSE: Provided is an anticancer composition comprising the alcohol extract of Pueraria sp. roots, which has excellent anticancer activity with less adverse effect and is thus useful for the prevention and treatment of cancer. CONSTITUTION: An anticancer composition is characterized by containing the alcohol extract of Pueraria sp. roots including Pueraria mirifica and Pueraria lobata. The alcohol extract is selected from PE1, PE2, PE3, PE4, Sub PE-A, Sub PE-B, Sub PE-C, Sub PE-D, Sub PE-E, Sub PE-F, Sub PE-G, Sub PE-H, Sub PE-D1, Sub PE-D2, and Sub PE-D3.

Abstract translation: 目的：提供包含葛根属酒精提取物的抗癌组合物。 根，具有优异的抗癌活性，副反应较少，因此可用于预防和治疗癌症。 构成：抗癌组合物的特征在于含有葛根属的酒精提取物。 根系包括葛根和葛根。 酒精提取物选自PE1，PE2，PE3，PE4，Sub PE-A，Sub PE-B，Sub PE-C，Sub PE-D，Sub PE-E，Sub PE-F，Sub PE-G，Sub PE-H，Sub-PE-D1，Sub-PE-D2，Sub-PE-D3。

公开(公告)号：KR100508627B1

公开(公告)日：2005-08-17

申请号：KR1020020015016

申请日：2002-03-20

Applicant: (주)케비젠

Inventor： 신홍식 ,  엄수종 ,  노영쇠 ,  박시호 ,  권윤자 ,  박명순 ,  한혜숙 ,  김소미 ,  김동명 ,  오덕근 ,  박종섭 ,  배태성

IPC: C07C235/78

Abstract: 본 발명은 화학식1로 표시되는 신규한 레티노이드 유도체 화합물 또는 이의 약제학적으로 허용되는 염에 관한 것이다:
화학식 1

상기식에서, X, R
1 , R
2 및 R
3 은 본원에 정의한 바와 같다. 또한, 본 발명은 상기 레티노이드 유도체 화합물의 제조 방법 및 그러한 화합물을 함유하는 항암제 조성물에 관한 것이다. 본 발명에 따른 화학식1의 화합물은 고도의 항암 활성을 나타내면서 유해한 부작용을 유발하지 않는다.

公开(公告)号：KR1020050033871A

公开(公告)日：2005-04-13

申请号：KR1020050014553

申请日：2005-02-22

Applicant: (주)케비젠

Inventor： 엄수종 ,  신홍식 ,  박시호 ,  백대헌 ,  전국체 ,  박명순 ,  박종섭 ,  노영쇠 ,  오덕근

IPC: A61K8/97 ,  A61Q19/00 ,  A61Q19/02 ,  A61Q19/08

Abstract: Provided are anticancer compositions comprising the alcohol extract of Pueraria sp. root. The compositions have excellent anticancer effects with less adverse side effects and is thus useful for prevention and treatment of various kinds of cancers. Therefore, the compositions are applied to anticancer-pharmaceuticals and cosmetics. The cosmetic composition comprises, as an active ingredient, the alcohol extract of Pueraria sp. root, wherein Pueraria sp. includes Pueraria mirifica and Pueraria lobata. The alcohol extract is at least one kind selected from PE1, PE2, PE3, PE4, Sub PE-A, Sub PE-B, Sub PE-C, Sub PE-D, Sub PE-E, Sub PE-F, Sub PE-G, Sub PE-H, Sub PE-D1, Sub PE-D2 and Sub PE-D3. The extract inhibits the proliferation of cancer cells to prevent and treat cancers. And it has skin stability, increases skin elasticity, has skin moisturizing effect and prevents skin wrinkles.

Abstract translation: 提供的是包含葛根属酒精提取物的抗癌组合物。 根。 该组合物具有优异的抗癌作用，副作用较小，因此可用于预防和治疗各种癌症。 因此，将组合物应用于抗癌药和化妆品。 化妆品组合物包含作为活性成分的葛根属酒精提取物。 根，其中葛根属 包括葛根和葛根。 酒精提取物选自PE1，PE2，PE3，PE4，Sub PE-A，Sub PE-B，Sub PE-C，Sub PE-D，Sub PE-E，Sub PE-F，Sub PE -G，Sub-PE-H，Sub-PE-D1，Sub-PE-D2和Sub-PE-D3。 提取物抑制癌细胞的增殖以预防和治疗癌症。 它具有皮肤稳定性，增加皮肤弹性，具有皮肤保湿效果，防止皮肤皱纹。

公开(公告)号：KR1020050023363A

公开(公告)日：2005-03-09

申请号：KR1020050004127

申请日：2005-01-17

Applicant: (주)케비젠

Inventor： 김소미 ,  오덕근 ,  백대헌 ,  신홍식 ,  박시호 ,  이유진 ,  엄수종 ,  노영쇠 ,  박종섭

IPC: C12N1/20

CPC classification number: C12P7/6427 ,  A23C9/1234 ,  A23C11/106 ,  A23L33/12 ,  A23L33/135 ,  A23P10/30 ,  A23Y2300/59 ,  A61K35/744 ,  A61K35/745 ,  C12R1/13

Abstract: PURPOSE: A conjugated linoleic acid(CLA) producing microorganism, a capsulated composition comprising the same microorganism, and the functional food using the same composition are provided, which microorganism has improved conjugated linoleic acid(CLA) producing activity, secretes CLA into the medium and accumulates CLA within the biomass, and has tolerance against acids such as acid in the stomach and bile juice and antibiotics. CONSTITUTION: The CLA producing microorganism which converts linoleic acid(LA) into conjugated linoleic acid(CLA) is provided, wherein the CLA producing microorganism is Bifidobacterium pseudocartenulatum CBG-C4(KACC 91003, KCTC 10208BP). The capsulated composition for prevention and treatment of cancer, arteriosclerosis, diabetes and obeseness comprises the CLA producing microorganism, wherein the coating material is water-soluble polysaccharides. The functional food containing the capsulated composition is provided, wherein the food is yoghurt, cheese, kimchi and jang(fermented soybean).

Abstract translation: 目的：提供共轭亚油酸（CLA）产生微生物，包含相同微生物的包封组合物和使用相同组成的功能性食品，该微生物具有改善的共轭亚油酸（CLA）生产活性，将CLA分泌到培养基中， 在生物质内积累CLA，并且具有对胃酸，胆汁和抗生素等酸的耐受性。 构成：提供将亚油酸（LA）转化为共轭亚油酸（CLA）的CLA产生微生物，其中CLA产生微生物是假双杆菌CBG-C4（KACC 91003，KCTC 10208BP）。 用于预防和治疗癌症，动脉硬化，糖尿病和肥胖症的包封组合物包括产生CLA的微生物，其中所述涂层材料是水溶性多糖。 提供含有封装组合物的功能性食品，其中食品是酸奶，奶酪，泡菜和姜（发酵大豆）。

公开(公告)号：KR100424251B1

公开(公告)日：2004-03-22

申请号：KR1020010025012

申请日：2001-05-08

Applicant: (주)케비젠 ,  신홍식

Inventor： 신홍식 ,  박명순 ,  노영쇠 ,  권윤자 ,  박시호 ,  엄수종 ,  장판식 ,  조성동

IPC: C07C227/18

Abstract: PURPOSE: A novel method for preparing α-L-aspartyl-L-phenylalanine methyl ester·HCl salt is provided, to reduce the amount of by-products, to allow the by-products to be reused after hydrolysis and to increase the production yield by 20% or more. CONSTITUTION: The method comprises the steps of reacting L-aspartic acid with trimethyl silyl chloride in the presence of an alcohol solvent to esterify the β-COOH selectively to obtain α-L-aspartyl-L-phenylalanine methyl ester·HCl. Preferably the alcohol solvent is selected from the group consisting of methyl alcohol, ethyl alcohol, allyl alcohol and benzyl alcohol. Preferably the method comprises further the steps of protecting an amine group with -CHO, Boc, benzyloxycarbonyl group, or -C(O)OR¬2 (R2 is methyl. ethyl or isobutyl group) by using acetic anhydride and formic acid.

Abstract translation: 目的：提供一种制备α-L-天冬氨酰-L-苯丙氨酸甲酯盐酸盐的新方法，以减少副产物的量，使副产物在水解后重新使用和 将产量提高20％以上。 构成：该方法包括以下步骤：在醇溶剂存在下使L-天冬氨酸与三甲基甲硅烷基氯化物反应以选择性酯化β-COOH以获得α-L-天冬氨酰-L-苯丙氨酸甲酯和amp ; middot; HCl中。 优选醇溶剂选自甲醇，乙醇，烯丙醇和苯甲醇。 优选地，该方法还包括通过使用乙酸酐和甲酸，用-CHO，Boc，苄氧基羰基或-C（O）OR 2（R 2为甲基，乙基或异丁基）保护胺基的步骤。

公开(公告)号：KR1020030081180A

公开(公告)日：2003-10-17

申请号：KR1020030023164

申请日：2003-04-12

Applicant: (주)케비젠

Inventor： 김소미 ,  오덕근 ,  백대헌 ,  신홍식 ,  박시호 ,  이유진 ,  엄수종 ,  노영쇠 ,  박종섭

IPC: C12N1/20

CPC classification number: C12P7/6427 ,  A23C9/1234 ,  A23C11/106 ,  A23L33/12 ,  A23L33/135 ,  A23P10/30 ,  A23Y2300/59 ,  A61K35/744 ,  A61K35/745 ,  C12R1/13

Abstract: PURPOSE: A novel microorganism capable of producing conjugated linoleic acid, a capsulated composition comprising the same, and a preparation method thereof are provided. The microorganism has high conjugated linoleic acid production yield and gastric juice and bile acid tolerance. CONSTITUTION: A microorganism capable of producing conjugated linoleic acid is provided, wherein the microorganism is Bifidobacterium breve CBG-C2 KACC 91001 and KCTC 10462BP, Bifidobacterium pseudocartenulatum CBG-C4 KACC 91003, and Enterococcus faecium CBG-C5 KACC 91002 and KCTC 10208BP. The capsulated composition for prevention and treatment of diseases capable of being inhibited by the conjugated linoleic acid, such as cancer, sclerosis of the arteries, diabetes and corpulence comprises the microorganism capable of producing conjugated linoleic acid.

Abstract translation: 目的：提供一种能够生产共轭亚油酸的新型微生物，包含其的包封组合物及其制备方法。 该微生物具有高共轭亚油酸产量和胃液和胆汁酸耐受性。 构成：提供能够产生共轭亚油酸的微生物，其中微生物是双歧双歧杆菌CBG-C2KACC91001和KCTC10462BP，假双歧杆菌CBG-C4KACC 91003和屎肠球菌CBG-C5 KACC 91002和KCTC 10208BP。 用于预防和治疗能够被共轭亚油酸抑制的疾病的包封组合物，例如癌症，动脉硬化，糖尿病和败血症包括能够产生共轭亚油酸的微生物。

公开(公告)号：KR100516377B1

公开(公告)日：2005-09-26

申请号：KR1020030023164

申请日：2003-04-12

Applicant: (주)케비젠

Inventor： 김소미 ,  오덕근 ,  백대헌 ,  신홍식 ,  박시호 ,  이유진 ,  엄수종 ,  노영쇠 ,  박종섭

IPC: C12N1/20

CPC classification number: C12P7/6427 ,  A23C9/1234 ,  A23C11/106 ,  A23L33/12 ,  A23L33/135 ,  A23P10/30 ,  A23Y2300/59 ,  A61K35/744 ,  A61K35/745 ,  C12R1/13

Abstract: 본 발명은 공역화 리놀레인산(CLA)을 생산할 수 있는 신규한 균주에 관한 것이다.
본 발명의 균주에는 비피도박테리움 브레베(
Bifidobacterium breve ) CBG-C2 균주, 비피도박테리움 슈도카르테눌라툼(
Bifidobacterium pseudocartenulatum ) CBG-C4 균주 및 엔테로코커스 패시움(
Enterococcus faecium ) CBG-C5 균주가 포함된다.
본 발명의 균주는 CLA 생산 능력이 우수하며, CLA를 생성하여 배지로 분비하는 한편 균체 내에 축적할 수 있다. 또한 본 발명의 균주는 위산이나 담즙 등의 산과 항생물질에 대해 강한 내성을 지닌다.
본 발명의 균주를 포함하는 조성물은 수용성 다당류로 이루어진 피복물질 내에 본 발명의 균주와 CLA를 포함하는 캡슐제 형태로 제조되어, 기능성 식품 및 의약품으로 사용될 수 있다.

公开(公告)号：KR100464061B1

公开(公告)日：2004-12-30

申请号：KR1020020026615

申请日：2002-05-15

Applicant: (주)케비젠

Inventor： 오덕근 ,  김소미 ,  신홍식 ,  엄수종 ,  노영쇠 ,  박종섭 ,  김동명 ,  박시호

IPC: C12P19/02

Abstract: PURPOSE: A method for producing tagatose by immobilization of arabinose isomerase is provided. Tagatose is cheaply and effectively mass-produced by maintaining the enzyme activity for a long time. CONSTITUTION: A method for producing tagatose by immobilization of arabinose isomerase comprises binding arabinose isomerase to resin, bridging arabinose isomerase with glutaraldehyde to immobilize the arabinose isomerase, and reacting the immobilized arabinose isomerase with substrate, wherein the concentration of arabinose isomerase is 10 mg/ml; the resin is sodium alginate, alumina or silica; the sodium alginate is used by dissolving 1.5 to 4.0% of sodium alginate in water; the alumina is used by dissolving 60 to 70% of alumina in 50 mM tris buffer solution; the silica is used by dissolving 60 to 70% of silica in 50 mM tris buffer solution; substrate is galactose; the galactose is used by dissolving 45 to 55% of galactose in pH 7 to 8 tris buffer solution; and the reaction temperature is 55 to 65 deg. C.

Abstract translation: 目的：提供一种通过固定阿拉伯糖异构酶来生产塔格糖的方法。 塔格糖通过长期维持酶活性而廉价且有效地大量生产。 构成：通过固定阿拉伯糖异构酶生产塔格糖的方法包括将阿拉伯糖异构酶与树脂结合，将阿拉伯糖异构酶与戊二醛桥接以固定阿拉伯糖异构酶，并使固定的阿拉伯糖异构酶与底物反应，其中阿拉伯糖异构酶的浓度为10mg / ml ; 该树脂是海藻酸钠，氧化铝或二氧化硅; 海藻酸钠通过在水中溶解1.5至4.0％的海藻酸钠来使用; 通过在50mM tris缓冲溶液中溶解60-70％的氧化铝来使用氧化铝; 二氧化硅是通过将60-70％的二氧化硅溶解在50mM tris缓冲溶液中而使用的; 底物是半乳糖; 半乳糖通过在pH 7至8 Tris缓冲溶液中溶解45-55％半乳糖来使用; 反应温度为55〜65℃。 C。

公开(公告)号：KR100437102B1

公开(公告)日：2004-06-23

申请号：KR1020020001178

申请日：2002-01-09

Applicant: (주)케비젠

Inventor： 신홍식 ,  박시호 ,  노영쇠 ,  엄수종 ,  권윤자 ,  박명순 ,  한혜숙 ,  정민숙 ,  김소미 ,  김동명 ,  오덕근 ,  박종섭

IPC: C07K5/00

Abstract: PURPOSE: Provided are a novel retinol derivative, its preparation method in higher yield, and its use. The novel retinol has excellent light-stability, and shows high reactivity to retinoic acid receptor α, while showing low reactivity to retinoic receptor β and γ. It can applied to medical products, cosmetics, soap, shampoo, functional foods, etc., for the prevention and improvement of skin aging. CONSTITUTION: The novel retinol derivative is characterized by carboester bond of a peptide material having COOH group, wherein the peptide material having COOH group is selected from -di, -tri, -poly peptide including N-L- α-aspartyl-L-phenylalanine 1-methylester(AMP;aspartame), N-protection group-aspartame, neotame and the like. Its manufacturing method comprises the steps of: reacting retinylacetate with methanolic solvent and inorganic slat at 25-40 deg.C in a dark room then extracting the reaction product with ether solvent; removing the solvent then followed by mixing a compound having OH group, natural or separated and purified retinol, diethylazodicarboxylate and triphenylphosphate with methylenechloride solvent, and reacting them at room temperature to obtain the ester derivative of retinol; and performing chromatography with reverse-phase, Merck Silicagel 60 RP 18(40-63) micro meter to separate pure ester derivative of retinol.

Abstract translation: 目的：提供一种新颖的视黄醇衍生物及其制备方法，其收率较高，并且其用途。 该新型视黄醇具有优异的光稳定性，并且对视黄酸受体α具有高反应性，而对视黄酸受体β具有低反应性; 和＆amp; gamma ;. 它可以应用于医疗产品，化妆品，肥皂，洗发水，功能性食品等，以预防和改善皮肤老化。 构成：新颖的视黄醇衍生物的特征在于具有COOH基团的肽材料的羧基酯键，其中具有COOH基团的肽材料选自包括NL-和α-天冬氨酰-L- 苯丙氨酸1-甲酯（AMP;天冬甜素），N-保护基 - 阿斯巴甜，纽甜等。 其制造方法包括以下步骤：在25-40℃，在黑暗的房间内将乙酸视黄醇酯与甲醇溶剂和无机薄片反应，然后用乙醚溶剂萃取反应产物; 然后除去溶剂，然后将具有OH基团的化合物，天然或分离纯化的视黄醇，偶氮二羧酸二乙酯和磷酸三苯酯与二氯甲烷溶剂混合，并在室温下反应，得到视黄醇的酯衍生物; 并用反相色谱（Merck Silicagel 60 RP 18（40-63）微米）进行色谱分离以分离视黄醇的纯酯衍生物。