Abstract:
A Bacillus humi analogous strain, a method for preparing the compost by using the Bacillus humi analogous strain, and the organic compost prepared by the method are provided to improve the quality of organic compost and to reduce the fermentation time. A Bacillus humi analogous strain comprises the sequence represented by the sequence number 1. A method for preparing the compost comprises the step of using the excretions of domestic animals and the mushroom culture waste sawdust culture medium in the roller system using the Bacillus humi analogous strain. Preferably the excretions of domestic animals and the mushroom culture waste sawdust culture medium are mixed in a ratio of 8:2.
Abstract:
본 발명은 한국 전통 간장으로부터 분리한 바실러스 퍼밀러스 HY1균주( Bacillus pumilus HY1 )를 동정하고 상기 균주로부터 생성되는 설펙틴의 분리 및 정제 방법, 상기 균주로부터 분리된 설펙틴의 유방암과 대장암에 대한 세포독성 시험 및 바실러스 퍼밀러스 ( Bacillus
pumilus ) HY1를 이용하여 설펙틴이 다량 함유된 청국장 및 간장의 속성 제조방법을 제공하는 뛰어난 효과가 있다. 바실러스 퍼밀러스, 설펙틴, 청국장, 간장 속성제조법
Abstract:
A novel multifunctional gene cel44C isolated from a Paenibacillus polymyxa GS01 strain is provided to decompose the cell wall of a plant and simultaneously decompose cellulose, xylan, lichenan and mannan, so that the gene is useful for paper industry and organic food production. The novel multifunctional gene cel44C isolated from a Paenibacillus polymyxa GS01 strain has the nucleotide sequence of SEQ ID NO:1, wherein the multifunctional enzyme encoded by the gene cel44C has the amino acid sequence of SEQ ID NO:2; the Paenibacillus polymyxa GS01 strain is isolated from the root of ginseng and is capable of decomposing plant cell wall; and the gene cel44C is analyzed by performing PCR(polymerase chain reaction) with a primer having any one nucleotide sequence selected from SEQ ID NO:3 to SEQ ID NO:8.
Abstract translation:提供了一种从多粘类芽孢杆菌GS01菌株中分离出的新型多功能基因cel44C,用于分解植物的细胞壁并同时分解纤维素,木聚糖,地衣多糖和甘露聚糖,使得该基因可用于造纸工业和有机食品生产。 从多粘类芽孢杆菌GS01菌株中分离的新型多功能基因cel44C具有SEQ ID NO:1的核苷酸序列,其中由基因cel44C编码的多功能酶具有SEQ ID NO:2的氨基酸序列; 多粘类芽孢杆菌GS01菌株是从人参的根中分离出来的,能够分解植物细胞壁; 并通过用具有选自SEQ ID NO:3至SEQ ID NO:8的任何一个核苷酸序列的引物进行PCR(聚合酶链式反应)来分析基因cel44C。
Abstract:
A Pseudomonas sp. 7014 strain is provided to shorten a total cultivation period of king oyster mushroom by promoting growth of mushroom mycelium and reduce manufacturing cost and labor cost significantly, thereby being very useful for cultivating other mushrooms as well as the king oyster mushroom, for gardening and for cultivating vegetables. A Pseudomonas sp. 7014 strain having the growth promoting activity during cultivation of bottle mushroom is deposited as a deposition no. KACC 91277P. A method for rapid cultivation of the bottle mushroom comprises the steps of: (a) preparing a culture medium by mixing the Oregon pine, Salix koreensis, wheat bran and rice bran in a volumetric ratio of 5-7:1-3:0.5-1.5:0.5-1.5, adding tap water thereto to adjust a moisture content to be 60-70%, filling up a plastic bottle with the mixture, applying mechanical perforation thereto and then sterilizing it; and (b) after inoculating the Pseudomonas sp. strain into the culture medium and allowing it to be fixated, inoculating mushroom sawdust spawn thereinto and culturing.
Abstract:
PURPOSE: Organophosphorus hydrolases effectively used for removing organophosphorus insecticide are provided to effectively decompose the organophosphorus insecticide and to confirm an optimized fermentation condition of young radish watery Kimchi with degrading lactic acid bacteria. CONSTITUTION: A method for fermenting of young radish watery Kimchi includes the following steps: salting young radish in salt water for 3 ~ 5 hours; washing the salted young radish; adjusting the slat concentration to 2 ~ 4 %; mixing wheat flour with water; manufacturing wheat flour liquid; mixing the wheat flour liquid with the young radish; making a seasoning for the kimchi; adding the seasoning in the young radish; and fermenting the young radish for 10 ~ 15 days.
Abstract:
PURPOSE: A method for detecting lactobacillus in Kimchi fermentation through multiplex PCR is provided to accurately and quickly detect grouping pattern of lactobacillus and recue time and cost. CONSTITUTION: A primer set for detecting lactobacillus in Kimchi fermentation using multiplex PCR comprises a primer pair of sequence number 1 and 2, 3 and 4, 5 and 6, 7 and 8, 9 and 10, 11 and 12, 13 and 14, 15 and 16, 17 and 18, or 19 and 20. A method for detecting lactobacillus in Kimchi fermentation comprises: a step of making a primer set which is specific to lactobacillus relating to Kimchi fermentation; a step of collecting a sample from Kimchi fermented 4°C~25°C in a specific time interval; a step of measuring change of strain number; a step of irritation; a step of confirming 10 kinds of lactobacillus by performing m-PCR; a step of confirming with a 16S rDNA(ribosomal DNA); and a step of detecting final m-PCR.
Abstract:
본 발명은 생육촉진 기능을 가지는 슈도모나스속 P7014 균주 및 이를 이용한 버섯 속성 재배방법에 관한 것으로, 새송이버섯 재배지로부터 식용버섯의 생육을 촉진하는 균을 분리하고 분리된 미생물을 동정한 후 감자한천배지 상에서 균사 생육촉진검정, 컬럼 테스트를 통한 생육촉진검정, 현미경 관찰을 통한 버섯과 분리균주의 상호작용 검정 및 병 재배 테스트를 통한 생육촉진 검정을 통해 상기 분리 미생물의 생육촉진능을 검정한 다음 상기 균주를 이용하여 식용버섯을 속성 재배한 후 재배일수 및 수확일수 단축을 조사함으로써 버섯을 속성으로 재배할 수 있게 하는 신규한 버섯 생육촉진능을 가지는 슈도모나스속 균주 및 이를 이용한 버섯 속성재배방법을 제공할 수 있는 매우 뛰어난 효과가 있다. 버섯생육촉진세균, 슈도모나스속, Pseudomonas sp., 식용버섯, 버섯속성재배
Abstract:
본 발명은 셀루로즈, 자이란, 리첸안 및 만난을 동시에 분해하는 다기능 단일효소의 효소활성을 증가시키는 방법에 관한 것으로 보다 상세하게는 본 발명은 셀루로즈, 자이란, 리첸안 및 만난을 동시에 분해하는 다기능 단일효소 Cel44C(미국국립생물정보센터, NCBI 등록번호 DQ367923)의 C-말단의 유전자 서열에 인공적인 종결 코돈을 넣은 프라이머를 제작하고 중합효소연쇄반응하여 상기 Cel44C의 셀룰라아제, 자이란아제, 리첸아제 및 만난아제의 활성을 증가시키는 매우 뛰어난 효과가 있다. Cel44C, 다기능 단일효소, 식물세포벽 분해효소, 효소활성 증가