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公开(公告)号：KR101346057B1
公开(公告)日：2013-12-31
申请号：KR1020120068585
申请日：2012-06-26
Applicant: 대한민국(농촌진흥청장) , 차의과학대학교 산학협력단
IPC: C07K1/14 , C07K14/435 , C12N1/04
Abstract: The present invention relates to a mass purification method of a silkworm hemolymph-derived protein and a protein purified thereby. The mass purification method according to the present invention comprises a first step of freeze-drying silkworm to prepare a silkworm dried material; a second step of removing silk gland and obtaining the remainder hemolymph; a third step of crushing the hemolymph and adding water thereto to prepare a hemolymph lysate; a fourth step of subjecting the hemolymph lysate to centrifugation to obtain a supernatant; and a fifth step of subjecting the supernatant to column chromatography to isolate a protein of 70kDa. According to the present invention, the silkworm hemolymph is applicable for several uses, such as a cell cryopreservative, a medium supplement for cell culture, and the like. Especially, the ingredients remaining after the isolation of silkworm hemolymph are also reusable, so that the present invention contributes to an increase in the income of sericulture farmers. [Reference numerals] (AA) Silk gland removal;(BB) Grown-up silkworm;(CC) live body weight 3.13g;(DD) Centrifugation;(EE) Quick freezing;(FF) Grown-up silkworm freeze-drying 590 mg;(GG) pulverization/selection;(HH) hemolymph 380 mg;(II) Dissolution (distilled water);(JJ) 63 mg after heat treatment;(KK) 30k 70k separation;(LL) Final retrieval amount
Abstract translation: 本发明涉及一种蚕血淋巴衍生蛋白的质量纯化方法和由此纯化的蛋白质。 根据本发明的质量净化方法包括：冷冻干燥蚕的第一步骤，制备蚕干物质; 去除丝腺并获得剩余血淋巴的第二步; 破碎血淋巴并向其中加入水以制备血淋巴溶胞产物的第三步骤; 第四步，使血淋巴溶胞产物离心得到上清液; 和将上清液进行柱色谱分离70kDa的蛋白质的第五步骤。 根据本发明，蚕血淋巴可应用于细胞冷冻保存剂，细胞培养用培养基等的多种用途。 特别是在分离蚕血淋淋后残留的成分也是可重复利用的，因此本发明有助于增加蚕食农民的收入。 （参考号）（AA）丝腺去除;（BB）成熟蚕;（CC）活体重3.13g;（DD）离心;（EE）快速冷冻;（FF）成熟蚕冷冻590 （GG）粉碎/选择;（HH）血淋巴380mg;（II）溶解（蒸馏水）;（JJ）热处理后63mg;（KK）30k 70k分离;（LL）最终检索量
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公开(公告)号：KR1020130049304A
公开(公告)日：2013-05-14
申请号：KR1020110114239
申请日：2011-11-04
Applicant: 대한민국(농촌진흥청장)
IPC: A61K31/7076 , A61K31/70 , A61K36/066 , A61P3/04
CPC classification number: A61K36/068 , A61K31/405 , A61K31/708 , A61K2236/333 , Y10S514/909
Abstract: PURPOSE: A pharmaceutical composition containing a Cordyceps militaris-derived compound for treating obesity is provided to ensure an excellent anti-obesity effect without side effects and toxicity. CONSTITUTION: A pharmaceutical composition for treating obesity contains two or more compounds selected among cordycepin, guanosine, and tryptophan derived from Cordyceps militaris. The compound is prepared by extracting Cordyceps militaris with a solvent and fractioning with butanol. The solvent is prepared by mixing water and ethanol in a volume ratio of 1:1. The composition suppresses differentiation of adipocytes.
Abstract translation: 目的：提供含有用于治疗肥胖症的冬虫夏草衍生化合物的药物组合物，以确保优异的抗肥胖效果而没有副作用和毒性。 构成：用于治疗肥胖症的药物组合物含有选自虫草素，鸟苷和来自冬虫夏草的色氨酸的两种或更多种化合物。 通过用溶剂萃取冬虫夏草并用丁醇分馏来制备化合物。 溶剂通过以1:1的体积比混合水和乙醇来制备。 该组合物抑制脂肪细胞的分化。
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公开(公告)号：KR101258699B1
公开(公告)日：2013-04-29
申请号：KR1020110040687
申请日：2011-04-29
Applicant: 대한민국(농촌진흥청장)
IPC: C12P7/62 , C12N1/14 , A61K31/215 , A61Q19/02
Abstract: 본 발명은 멜라닌 생성억제물질 에스-디하이드록시팔네식산 메틸 에스터의 대량생산방법에 관한 것이다.
본 발명의 멜라닌 생성억제물질의 대량생산방법은 곤충유래 곰팡이를 배양하여 종균 배양액을 제조하는 단계 및 상기 종균 배양액을 글루코스(glucose), 프락토스(fructose), 슈크로스(sucrose), 덱스트린(dextrin) 중 선택된 어느 하나 이상의 탄소원과 트립톤(tryptone), 콘스팁리쿼(CSL, corn steep Liquor) 중 선택된 어느 하나 이상의 질소원을 포함하는 배지에 접종한 후, 이를 배양하여 멜라닌 생성억제물질을 대량생산하는 단계를 포함한다.
본 발명에 의해, 멜라닌 생성억제 활성이 뛰어난 에스-디하이드록시팔네식산 메틸 에스터(S-(-)-10,11-Dihydroxyfarnesic acid methyl ester)의 대량생산이 가능하다.-
公开(公告)号：KR1020120122497A
公开(公告)日：2012-11-07
申请号：KR1020110040687
申请日：2011-04-29
Applicant: 대한민국(농촌진흥청장)
IPC: C12P7/62 , C12N1/14 , A61K31/215 , A61Q19/02
CPC classification number: C12P7/62 , A61K8/33 , A61K31/215 , A61Q19/02 , C12N1/14
Abstract: PURPOSE: A method for producing a large amount of S-dihydroxyfarnesic acid methyl ester is provided to obtain the compound with excellent melanogenesis prevention. CONSTITUTION: A method for producing a large amount melanogenesis inhibitors comprises: a step of culturing insect-derived fungi and preparing a spawn culture medium; a step of inoculating the spawn culture medium to a medium containing a carbon source of glucose, fructose, sucrose, or dextrin and a nitrogen source of tryptone or CSL(corn steep liquor); a step of culturing the mixture. The spawn culture medium is prepared by culturing an insect-derived fungi-inoculated medium at 25-27 Deg. C. for 5-7 days; and a step of inoculating the fungi culture at 160-180 rpm and 25-27 Deg. C. for 3-4 days. A melanogenesis inhibitor contains a melanogenesis inhibiting material, S-(-)-10,11-Dihydroxyfarnesic acid methyl ester.
Abstract translation: 目的：提供大量的S-二羟基法氢酸甲酯的制造方法，以获得预防黑素生成优异的化合物。 构成：生产大量黑素生成抑制剂的方法，包括：培养昆虫来源的真菌并制备产卵培养基的步骤; 将产卵培养基接种到含有葡萄糖，果糖，蔗糖或糊精的碳源和胰蛋白胨或CSL（玉米浆）的氮源的培养基中的步骤; 培养混合物的步骤。 产卵培养基是通过在25-27度培养昆虫来源的真菌接种培养基来制备的。 C. 5-7天; 并以160-180rpm和25-27℃接种真菌培养物的步骤。 C. 3-4天。 黑素生成抑制剂含有黑素生成抑制物质S - （ - ） - 10,11-二羟基法呢酸甲酯。
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公开(公告)号：KR101165041B1
公开(公告)日：2012-07-20
申请号：KR1020100021038
申请日：2010-03-09
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 살아 있는 누에번데기를 이용하여 밀리타리스동충하초의 자실체를 생산하는 방법으로, 밀리타리스동충하초를 보다 효율적으로 증식하기 위한 접종원 제조방법, 접종방법, 접종시기 및 배양 방법 등에 관한 것이다. 본 발명에 따르면, 살아있는 누에번데기에서 밀리타리스동충하초의 감염률 및 자실체의 발생률을 높일 수 있으며, 접종원 접종량, 접종방법, 접종시기, 배양방법 등을 표준화하여 보다 효율적이고 편리하게 재배할 수 있는 가장 경제적인 밀리타리스동충하초 자실체의 배양방법이 제공될 수 있다.
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Patent Agency Ranking
公开(公告)号：KR1020120071546A
公开(公告)日：2012-07-03
申请号：KR1020100133134
申请日：2010-12-23
Applicant: 대한민국(농촌진흥청장)
CPC classification number: A61L27/227 , A61F2/0063 , A61L2430/32 , D04H1/09
Abstract: PURPOSE: A silk artificial lipid membrane for artificial cerebral dura mater and biological films are provided to facilitate surgical operation by reinforcing flexibility and improving stability. CONSTITUTION: A silk artificial lipid membrane for artificial cerebral dura mater and biological films comprises the following steps: manufacturing a mixed solution by mixing 0.01-50.00 wt% of salt with 50-99.99wt% of silk fibroin; and manufacturing a silk artificial lipid membrane by pouring the mixed solution into a flat container and desiccating thereof at 20-80 deg. Celsius in a thermostatic chamber which contains 20-80 wt% of moisture. The salt is one of calcium chloride, sodium chloride, potassium chloride, and sodium acetate.
Abstract translation: 目的：提供用于人造脑硬膜和生物膜的丝素人造脂膜，以通过增强弹性和提高稳定性来促进外科手术。 构成：用于人造脑硬膜和生物膜的丝素人造脂膜包括以下步骤：通过将0.01-50.00重量％的盐与50-99.99重量％的丝素蛋白混合制备混合溶液; 并通过将混合溶液倒入平坦容器中并在20-80℃干燥而制造丝状人造脂质膜。 摄氏度在含有20-80重量％水分的恒温槽中。 该盐是氯化钙，氯化钠，氯化钾和乙酸钠之一。
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公开(公告)号：KR1020110102863A
公开(公告)日：2011-09-19
申请号：KR1020110088887
申请日：2011-09-02
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6883 , C12Q1/686
Abstract: 본 발명은 녹강병 및 백강병 진단용 프라이머 및 이를 이용한 진단방법에 관한 것으로 보다 상세하게는 유용곤충(꽃무지 및 장수풍뎅이)에 주로 발생하는 곰팡이인 녹강병원균(
Metarhizium
anisopliae
,
메타리지움
아니소플리애 )와 백강병원균(
Beauveria bassiana, 뷰베리아 바시아나 )을 종 특이적으로 각각 또는 동시에 PCR을 통하여 진단할 수 있는 진단용 프라이머 및 이를 이용한 진단 방법에 관한 것이다. 본 발명의
메타리지움
아니소플리애 또는
뷰베리아
바시아나 병원균 검출방법은 상기 병원균에 종 특이적으로 결합하는 뉴클레오타이드 서열을 이용하기 때문에, 극소량의 병원균이 존재하여도 이를 조기에 정확한 감도로 검출할 수 있다. 또한, 상기 병원균을 각각의 절차에 의해 검출하거나 단지 한 번의 절차만으로 동시에 검출할 수 있는 장점을 갖기 때문에 식약용 곤충에 가장 문제가 되는
메타리지움
아니소플리에
또는
뷰베리아
바시아나 병원균 예방과 방제에 시간과 비용을 절약하여 자원화 곤충의 안정적인 대량생산에 기여할 수 있다.-
公开(公告)号：KR1020110024025A
公开(公告)日：2011-03-09
申请号：KR1020090081867
申请日：2009-09-01
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/686 , C12N15/11 , C12Q1/689 , C12Q2600/16
Abstract: PURPOSE: A primer for diagnosing green muscardine and white muscardine is provided to accurately detect in presence of a small amount of pathogen. CONSTITUTION: A PCR primer set for diagnosing green muscardine comprises NC-F1 primer(sequence number 1) and NC-R1 primer(sequence number 2). A PCR kit for detecting Metarhizium anisopliae contains the PCR primer set. A method for diagnosing Metarhizium anisopliae infection comprises: a step of preparing DNA from a sample; and a step of performing PCR using the primer set. A PCR primer set for white muscardine comprises J-1 primer(sequence number 3) and J-2 primer(sequence number 4).
Abstract translation: 目的：提供用于诊断绿色麝香碱和白麝香碱的底漆，以准确检测少量病原体的存在。 构成：用于诊断绿色肌肉的PCR引物组包括NC-F1引物（序列号1）和NC-R1引物（序列号2）。 用于检测Metarhizium anisopliae的PCR试剂盒含有PCR引物组。 诊断Metarhizium anisopliae感染的方法包括：从样品制备DNA的步骤; 以及使用引物组进行PCR的步骤。 用于白麝香碱的PCR引物组包括J-1引物（序列号3）和J-2引物（序列号4）。
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公开(公告)号：KR100758813B1
公开(公告)日：2007-09-19
申请号：KR1020050133381
申请日：2005-12-29
Applicant: 대한민국(농촌진흥청장)
Abstract: A method for preserving pupa infected with Cordyceps spp, entomopathogenic fungi for a long time and a method for regenerating the fungi are provided to dispense with an expensive equipment or high technology for preserving the fungi, and also to produce fruit bodies on consumer demands by taking out the preserved pupa at desired time. The cultivation time of Cordyceps spp, entomopathogenic fungi can be controlled voluntarily, thereby solving the concentration of production time and the surplus or stock of production. A long-term preserving method of pupa infected with Cordyceps spp, entomopathogenic fungi comprises: preparing the pupa infected with Cordyceps spp, entomopathogenic fungi, controlling the water content of the pupa body to 4%, and then preserving the pupa at 4°C or less, or at -70°C after freeze-drying. The fungi regenerating method comprises soaking the preserved pupa in distilled water for 1~3hr, followed by saturating the pupa with water. The infected pupa can be preserved stably in a water content of 4% at a storage temperature of 4°C or less for 135day, and the regeneration ratio is to about 97%. In addition, a preservation period is more increased at lower temperature in freezing, and is up to 1year in preserving at -70°C or less after freeze-drying.
Abstract translation: 提供了一种用于保存长时间感染冬虫夏草，昆虫病原真菌的蛹的方法和用于再生真菌的方法，以省去用于保存真菌的昂贵的设备或高技术，以及通过采取消费者需求来生产果实 在所需的时间出来保存的蛹。 冬虫夏草，昆虫病原菌的培养时间可以自愿控制，从而解决生产时间和生产过剩或库存的集中。 昆虫病原真菌感染虫蛹的长期保存方法包括：制备感染冬虫夏草，昆虫病原真菌的蛹，将蛹体的水含量控制在4％，然后在4℃保存蛹，或 较少，或在-70℃下冷冻干燥。 真菌再生方法包括将保存的蛹在蒸馏水中浸泡1〜3小时，然后用水饱和蛹。 感染蛹可以在4℃或更低的储存温度下稳定保存在4％的水含量中，135天，再生率为约97％。 另外，冷冻时的温度保持时间更长，冷冻干燥后在-70℃以下保存1年左右。
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公开(公告)号：KR1020030012998A
公开(公告)日：2003-02-14
申请号：KR1020010047271
申请日：2001-08-06
Applicant: 대한민국(농촌진흥청장)
IPC: A01G1/04
CPC classification number: A61K36/068
Abstract: PURPOSE: A method for culturing Isaria sinclairii is provided by inoculating Isaria sinclairii to the pupa or larva of a domestic silkworm, thereby mass producing Isaria sinclairii . CONSTITUTION: The method for culturing Isaria sinclairii using a domestic silkworm comprises the steps of: inoculating spores of Isaria sinclairii to the larva or pupa of a domestic silkworm by spraying the spores on the surface of the larva or the pupa; and forming a fruit body of Isaria sinclairii from the infected pupa, wherein the inoculation process is carried out by diluting the spores of Isaria sinclairii in water in the concentration of 106/ml to 108/ml, spraying the diluted solution on the surfaces of the larvae or pupae of the domestic silkworms aged 2 weeks, 3 weeks, 4 weeks and 5 weeks, and leaving them under 95% of humidity for 12 hours; the larvae or pupae of the domestic silkworms are stored at 20 deg. C or less prior to the inoculation; and the fruit body formation process is carried out under conditions of 15 to 25 deg. C and humidity of 95%.
Abstract translation: 目的：通过将Isaria sinclairii接种到家蚕的蛹或幼虫来提供Isaria sinclairii的培养方法，从而大量生产Isaria sinclairii。 构成：使用家蚕培养伊蚊（Isaria sinclairii）的方法包括以下步骤：通过将孢子喷洒在幼虫或蛹的表面上，将Isaria sinclairii的孢子接种到家蚕的幼虫或蛹中; 并从感染的蛹中形成Isaria sinclairii的果实体，其中通过在浓度为106 / ml至108 / ml的水中稀释Isaria sinclairii的孢子进行接种过程，将稀释的溶液喷洒在 家蚕的幼虫或蛹2周，3周，4周和5周，并将其置于95％湿度下12小时; 家蚕的幼虫或蛹保存在20度。 C以下; 果实体形成处理在15〜25℃的条件下进行。 C和湿度95％。
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