公开(公告)号：KR101433362B1

公开(公告)日：2014-08-26

申请号：KR1020130012481

申请日：2013-02-04

Applicant: 한국식품연구원 ,  고려대학교 산학협력단

Inventor： 최희돈 ,  김윤숙 ,  박호영 ,  하상근 ,  이상훈 ,  홍희도 ,  이광원 ,  홍승택 ,  남미현 ,  양재언

IPC: A23L1/305 ,  A23L1/30 ,  A61K38/38 ,  A61K31/721

Abstract: 본 발명은 알파-락트알부민과 덱스트란의 마이얄반응을 통해 생성된 당단백질을 유효성분으로 함유하는 면역 증강용 조성물에 관한 것으로, 더 상세하게는 알파-락트알부민(alpha-lactalbumin)과 덱스트란(dextran)의 마이얄반응(Maillard reaction)을 통해 생성된 당단백질을 유효성분으로 함유하는 면역 증강용 또는 암 개선용 식품용 조성물, 면역력 저하로 인한 질환 치료용 또는 암 치료용 약학적 조성물에 관한 것이다.
본 발명의 당단백질은 면역 증진에 필요한 사이토킨 및 백혈구의 수를 늘려주고, 종양을 억제하는 효능이 있어, 면역 증진용 조성물 및 암 치료용 조성물의 제조에 효과적이다.

公开(公告)号：KR1020140099991A

公开(公告)日：2014-08-14

申请号：KR1020130012481

申请日：2013-02-04

Applicant: 한국식품연구원 ,  고려대학교 산학협력단

Inventor： 최희돈 ,  김윤숙 ,  박호영 ,  하상근 ,  이상훈 ,  홍희도 ,  이광원 ,  홍승택 ,  남미현 ,  양재언

IPC: A23L1/305 ,  A23L1/30 ,  A61K38/38 ,  A61K31/721

CPC classification number: A23L33/17 ,  A23V2002/00 ,  A23V2200/324 ,  A61K31/721 ,  A61K38/38

Abstract: The present invention relates to a composition for immune enhancement containing, as an active ingredient, glycoprotein produced through a Maillard reaction of alpha-lactalbumin and dextran, and more specifically, to a composition for immune enhancement or a food composition for cancer alleviation, containing, as an active ingredient, glycoprotein produced through a Maillard reaction of alpha-lactalbumin and dextran, and to a pharmaceutical composition for treating disease caused by decreased immunity or treating cancer. The glycoprotein of the present invention has an effect of increasing the number of cytokines and leukocytes, which are necessary for immunological enhancement, and suppressing tumor, and thus is effective in preparing a composition for immune enhancement and a composition for cancer treatment.

Abstract translation: 本发明涉及一种用于免疫增强的组合物，其含有通过α-乳白蛋白和葡聚糖的美拉德反应产生的糖蛋白作为活性成分，更具体地，涉及用于免疫增强的组合物或用于癌症缓解的食物组合物， 作为活性成分，通过α-乳清蛋白和葡聚糖的美拉德反应产生的糖蛋白，以及用于治疗由免疫力降低或治疗癌症引起的疾病的药物组合物。 本发明的糖蛋白具有增加免疫增强所必需的细胞因子和白细胞数量以及抑制肿瘤的效果，因此有效地制备免疫增强用组合物和癌症治疗用组合物。

公开(公告)号：KR101419706B1

公开(公告)日：2014-07-15

申请号：KR1020120156213

申请日：2012-12-28

Applicant: 한국식품연구원

Inventor： 최인욱 ,  박용곤 ,  하상근 ,  최희돈 ,  임진선

IPC: A61K31/352 ,  A61K31/045 ,  A61K31/05 ,  A61P29/00

Abstract: 본 발명은, 루테올린에 하기 화학식 1로 표시되는 카테콜기를 작용기로 포함하는 화합물을 첨가함으로써 생체내에서의 항염증 효능이 증가된 루테올린 혼합 조성물을 제조하는 방법에 관한 것이다.
본 발명에 의하면, 생체내에서의 루테올린의 항염증 효능을 증가시키기 위하여 특정한 구조의 천연 화합물을 생체내 루테올린 간 대사의 competitor로서 혼합하는 혼합 조성물의 제조방법 및 그 제조방법에 의하여 제조된 생체내에서의 항염증 효능이 증가된 루테올린 혼합 조성물을 제공할 수 있다.

公开(公告)号：KR1020140066821A

公开(公告)日：2014-06-02

申请号：KR1020120132891

申请日：2012-11-22

Applicant: 한국식품연구원

Inventor： 최희돈 ,  김윤숙 ,  박호영 ,  하상근 ,  이상훈 ,  유아름 ,  홍희도

IPC: C07K1/34 ,  C07K1/36 ,  C07K14/195 ,  C07K14/37

Abstract: The present invention relates to a fraction of rice wine lees derived-glycoprotein, which is characterized in having a molecular weight of 15 to 110 kDa, and a method of producing the same and, more specifically, to a fraction of rice wine lees-derived glycoprotein and the uses thereof, which is prepared by a method comprising: (a) a step of removing fat in rice wine lees; (b) a step of mixing the rice wine lees in a buffer solution having a pH level of 7.0 to 8.0; (c) a step of obtaining a supernatant by centrifugation; (d) a step of dialyzing the supernatant with a dialysis semipermeable membrane (MWCO 6000-8000); (e) a step of adding a salt precipitating protein in the dialyzed supernatant to precipitate glycoprotein; (f) a step of dissolving the precipitate in a buffer solution having a pH level of 7.0 to 8.0 with a dialysis semipermeable membrane (MWCO 6000-8000); and (g) a step of obtaining a fraction of glycoprotein from the dialyzed and precipitated lysate. The fraction of glycoprotein of the present invention has a better effectiveness of promoting histiocyte vitality than Krestin (PSK), which is a medicine for enhancing immune vitality, and has no cytotoxicity, thereby being effective in producing a new functional food and a medicine for enhancing immune function.

Abstract translation: 本发明涉及分子量为15〜110kDa的米酒衍生糖蛋白的一部分及其制造方法，更具体地说，涉及一批来自米酒的糖酵母 糖蛋白及其用途，其通过以下方法制备：（a）在米酒中除去脂肪的步骤; （b）将米酒混合在pH为7.0〜8.0的缓冲液中的工序; （c）通过离心获得上清液的步骤; （d）用透析半透膜（MWCO 6000-8000）透析上清液的步骤; （e）在透析的上清液中加入沉淀蛋白质沉淀糖蛋白的步骤; （f）用透析半透膜（MWCO 6000-8000）将沉淀物溶解在pH值为7.0〜8.0的缓冲溶液中的步骤; 和（g）从透析和沉淀的裂解物中获得一部分糖蛋白的步骤。 与作为增强免疫活力的药物Krestin（PSK）相比，本发明的糖蛋白的分数具有更好的促进组织细胞活力的效果，并且没有细胞毒性，因此有效地生产新的功能性食品和增强药物 免疫功能。

公开(公告)号：KR1020130076102A

公开(公告)日：2013-07-08

申请号：KR1020110144529

申请日：2011-12-28

Applicant: 한국식품연구원

Inventor： 최희돈 ,  박용곤 ,  최인욱 ,  김윤숙 ,  박호영 ,  하상근 ,  이상훈 ,  나세준 ,  최태동

IPC: A23L1/30 ,  A23L19/00 ,  A23L5/40

CPC classification number: A23L19/00 ,  A23V2002/00 ,  A23V2300/14 ,  A23V2300/44

Abstract: PURPOSE: A separating method is provided to extract capsaicinoid and carotenoid from red pepper without using organic solvent, and to prevent the denaturation and destruction of effective component. CONSTITUTION: (a) One to four percent of yield fraction is collected from red pepper by supercritical carbon dioxide extraction on condition that the temperature is 30-50°C and the pressure is 1500-2500 psi. (b) One to four percent of yield fraction is collected from red pepper of (a) step by supercritical carbon dioxide extraction on condition that the temperature is 30-50°C and the pressure is 6000-7500 psi. (c) One to four percent of yield fraction is collected from the red pepper by supercritical carbon dioxide extraction on condition that the temperature is 30-50°C and the pressure is 6000-7500 psi. The pressure condition in (a) step is 1500-2000 psi. The temperature condition in (a) step is 35-45°C. The temperature condition in (b) step is 35-45°C.

Abstract translation: 目的：提供从红辣椒提取辣椒素和类胡萝卜素而不使用有机溶剂的分离方法，并防止有效成分的变性和破坏。 构成：（a）在温度为30-50℃，压力为1500-2500psi的条件下，通过超临界二氧化碳萃取从红辣椒中收集1％至4％的产率。 （b）在温度为30-50℃，压力为6000-7500psi的条件下，通过超临界二氧化碳萃取从（a）步骤的红辣椒中收集1％至4％的收率级分。 （c）在温度为30-50℃，压力为6000-7500psi的条件下，通过超临界二氧化碳萃取从红辣椒中收集1％至4％的产率。 （a）步骤中的压力条件为1500-2000psi。 （a）步骤中的温度条件为35-45℃。 （b）步骤的温度条件为35-45℃。

公开(公告)号：KR1020130076091A

公开(公告)日：2013-07-08

申请号：KR1020110144516

申请日：2011-12-28

Applicant: 한국식품연구원

Inventor： 최희돈 ,  박용곤 ,  최인욱 ,  김윤숙 ,  박호영 ,  하상근 ,  이상훈 ,  나세준 ,  최태동

IPC: A23L27/00 ,  A23L27/10

CPC classification number: A23L27/10 ,  A23L29/06 ,  A23V2002/00 ,  A23V2250/21

Abstract: PURPOSE: A manufacturing method is provided to manufacture spice and the taste component of red pepper without the denaturation and the destruction of effective component. CONSTITUTION: Red pepper extract and the extract remnant are manufactured by supercritical extraction. It is the supercritical extraction and the red pepper extract and extracted cake is manufactured. An enzymatic lysate is manufactured by reacting the produced extract remnant with at least one of cell wall enzyme or proteinase. The extract includes oleoresin The oleoresin is added to the enzymatic lysate and mixed together. The supercritical extraction condition is as in the following: 35-45°C of temperature; 4500-7500 psi; and 5-10 of CO2 / sample rate. The cell wall degrading enzyme is viscozyme, celluclast, pectinex, or ultraflo. The proteinase is alcalase or protamex. The Reaction occurs at 45-55°C. The spice composition includes the enzymatic lysate. The enzymatic lysate comprises at least one of amino nitrogen, reducing sugar or organic acid.

Abstract translation: 目的：提供制造香料和红辣椒味道成分的制造方法，而不会使变质和破坏有效成分。 构成：红辣椒提取物和提取物残留物通过超临界萃取法生产。 是超临界萃取，红辣椒提取物和提取的蛋糕制造。 通过使产生的提取物残留物与细胞壁酶或蛋白酶中的至少一种反应来制备酶裂解物。 提取物包括油树脂。将油树脂加入到酶裂解物中并混合在一起。 超临界萃取条件如下：温度35-45℃; 4500-7500 psi; 和5-10的CO2 /采样率。 细胞壁降解酶是粘蛋白酶，纤维素，果胶或超细胞。 蛋白酶是alcalase或protamex。 反应发生在45-55℃。 香料组合物包括酶裂解物。 酶裂解物包含氨基氮，还原糖或有机酸中的至少一种。