公开(公告)号：TR200607443T1

公开(公告)日：2007-04-24

申请号：TR200607443

申请日：2000-06-23

Applicant: BASF AG

Inventor： POMPEJUS MARKUS ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  ZELDER OSKAR ,  HABERHAUER GREGOR

IPC: C12N15/31 ,  G01N33/53 ,  C07K20060101 ,  C07K14/34 ,  C12N20060101 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/18 ,  C12N15/09 ,  C12N15/52 ,  C12P20060101 ,  C12P1/04 ,  C12P13/04 ,  C12P21/02 ,  C12Q20060101 ,  C12Q1/04 ,  C12Q1/68 ,  C12R1/13 ,  C12R1/15 ,  C12R1/16 ,  G01N33/569

Abstract: Isolated nucleic acid molecules, designated MCT nucleic acid molecules, which encode novel MCT proteins from Corynebacterium glutamicum are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing MCT nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides isolated MCT proteins, mutated MCT proteins, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from C. glutamicum based on genetic engineering of MCT genes in this organism.

公开(公告)号：TR200700068T2

公开(公告)日：2007-03-21

申请号：TR200700068

申请日：2000-06-27

Applicant: BASF AG

Inventor： POMPEJUS MARKUS ,  KROEGER BURKHARD ,  HARBERHAUER GREGOR ,  ZELDER OSKAR ,  SCHROEDER HARTWIG

IPC: C12N15/54 ,  G01N33/53 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/10 ,  C12N9/12 ,  C12N15/09 ,  C12N15/31 ,  C12P1/04 ,  C12P13/04 ,  C12Q1/04 ,  C12Q1/68 ,  C12R1/13 ,  C12R1/16 ,  G01N33/566 ,  G01N33/569

Abstract: Isolated nucleic acid molecules, designated PTS nucleic acid molecules, which encode novel PTS proteins from Corynebacterium glutamicum are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing PTS nucleic acid molecules, and host cells into which expression vectors have been introduced. The invention still further provides isolated PTS proteins, mutated PTS proteins, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from C. Glutamicum based on genetic engineering of PTS genes in this organism.

公开(公告)号：CA2620468A1

公开(公告)日：2007-02-22

申请号：CA2620468

申请日：2006-08-18

Applicant: BASF AG

Inventor： KROEMER JENS ,  SCHROEDER HARTWIG ,  WITTMANN CHRISTOPH ,  HEINZLE ELMAR ,  HAEFNER STEFAN ,  HEROLD ANDREA ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  PERO JANICE ,  YOCUM ROGERS ,  WILLIAMS MARK ,  HERMAN THERON ,  PATTERSON THOMAS

IPC: C12N15/52 ,  C12P13/12

Abstract: The present invention relates to methods for the production of microorganisms with increased efficiency for methionine synthesis, microorganisms with increased efficiency for methionine synthesis, and methods for determining the optimal metabolic flux for organisms with respect to methionine synthesis.

公开(公告)号：ZA200404418B

公开(公告)日：2006-07-26

申请号：ZA200404418

申请日：2004-06-04

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  POMPEJUS MARKUS ,  SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  KLOPPROGGE CORINNA ,  HABENHAUER GREGOR

IPC: C12N15/11 ,  C07K14/34 ,  C12N20060101 ,  C12N1/21 ,  C12N15/63 ,  C12P13/08

Abstract: Isolated nucleic acid (I) that encodes particular protein sequences (II) that have, at specific positions, an amino acid alteration. Independent claims are also included for: (1) Vector that contains at least one (I); (2) Host cell transfected with at least one vector of (1); and (3) Method for preparing fine chemicals by culturing cells of (2).

公开(公告)号：ZA200404420B

公开(公告)日：2006-05-31

申请号：ZA200404420

申请日：2004-06-04

Applicant: BASF AG

Inventor： HABERHAUER GREGOR ,  ZELDER OSKAR ,  POMPEJUS MARKUS ,  SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  KLOPPROGGE CORRINA

IPC: C12N20060101 ,  C12N1/21 ,  C12N9/12 ,  C12N15/21 ,  C12N15/31 ,  C12N15/54 ,  C12P20060101

Abstract: Isolated nucleic acid (I) that encodes any of 4 amino acid sequences (II) that have, at specific positions, an amino acid (aa) alteration are new. The specification includes (i) a table identifying the function of (II) and the nature/position of the amino acid exchange and (ii) sequences for (I) and (II). Independent claims are also included for the following: (1) Vector that contains at least one (I); (2) Host cell (C) transfected with at least one vector of (1); and (3) Preparing fine chemicals by culturing cells of (C).

公开(公告)号：AR047152A1

公开(公告)日：2006-01-11

申请号：ARP040104737

申请日：2004-12-17

Applicant: BASF AG

Inventor： KROEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

IPC: C12N9/02 ,  C12N15/77 ,  C12N15/31 ,  C12N15/67 ,  C12N15/63 ,  C12P13/08 ,  C12P13/12 ,  C12R1/15 ,  C12N1/21 ,  C12R1/13

Abstract: Se refiere al uso de secuencias de ácidos nucleicos para la regulacion de la transcripcion y la expresion de genes, a los nuevos promotores y unidades de expresion propiamente dichos, a procedimientos para la modificacion o activacion del grado de transcripcion y/o grado de expresion de genes, a casetes de expresion que contienen las unidades de expresion, a microorganismos modificados genéticamente con grado de transcripcion y/o grado de expresion modificado o activado, así como también a procedimientos para la preparacion de productos biosintéticos por medio del cultivo de los microorganismos genéticamente modificados. Reivindicacion 1: Uso de un ácido nucleico con actividad promotora, que contiene: A) la secuencia de ácidos nucleicos SEQ. ID. NO. 1 o B) una secuencia derivada de esta secuencia por sustitucion, insercion o delecion de nucleotidos, la que presenta una identidad de por lo menos 90 % a nivel de ácidos nucleicos con la secuencia SEQ. ID. NO. 1, o C) una secuencia de ácidos nucleicos, que hibrida con la secuencia de ácidos nucleicos SEQ. ID. NO. 1 bajo condiciones severas o D) fragmentos funcionalmente equivalentes de las secuencias de A), B) o C), para la transcripcion de genes.

公开(公告)号：AR047058A1

公开(公告)日：2006-01-04

申请号：ARP040104742

申请日：2004-12-17

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  KROEGER BURKHARD ,  KIEFER PATRICK ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH

IPC: C12P20060101 ,  C12P13/08 ,  C12N15/54 ,  C12N1/21 ,  C12R1/15

Abstract: Comprende métodos para aumentar la produccion de un compuesto químico fino, por ejemplo, lisina a partir de un microorganismo, por ejemplo, Corynebacterium mediante la desregulacion de un gen codificador de una enzima, es decir, glicerol quinasa. Se proveen métodos para incrementar la produccion de lisina en Corynebacterium glutamicum a través del incremento de la expresion de la actividad de glicerol quinasa. También se provee un proceso para la produccion de lisina a través de la regulacion del flujo de carbono oxalacetato (OAA).Se proveen métodos para la produccion de lisina a través de la utilizacion de fructosa o sacarosa como fuente de carbono. Reivindicacion 1: Un método para aumentar el flujo metabolico a través de la vía de pentosafosfatos en un microorganismo que comprenda el cultivo de dicho microorganismo que contenga un gen que se desregula bajo condiciones tales que se aumenta el flujo metabolico a través de la vía de pentosafosfatos. Reivindicacion 49: Un microorganismo recombinante que comprende un gen de biosíntesis de pentosafosfato desregulado.

公开(公告)号：DE10359595A1

公开(公告)日：2005-07-28

申请号：DE10359595

申请日：2003-12-18

Applicant: BASF AG

Inventor： KROEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

IPC: C07K14/34 ,  C12N15/77 ,  C12P13/08 ,  C12N15/31 ,  C12N1/00

Abstract: Use of a nucleic acid (I) with promoter activity for transcribing genes, where (I) is (a) a 164 bp sequence (SEQ ID No,:1); (b) a variant of (SEQ ID No.:1) with at least 90% identity and derived by substitution, insertion or deletion of nucleotides; (c) a sequence that hybridizes to (SEQ ID No.:1) under stringent conditions; or (d) a functionally equivalent fragment of (a)-(c), is new. Independent claims are also included for : (1) use of an expression unit (EU), containing (I) and functionally linked to a sequence (X) that ensures translation of RNA, for expressing genes; (2) (I), other than sequence (1), i.e. (Ia), as new compounds; (3) EU that contain (Ia) linked to (X); (4) altering (or producing) the transcription rate of genes in a microorganism, relative to the wild type; (5) expression cassette (EC) comprising at least one EU of (1), at least one other nucleic acid sequence (to be expressed) and optionally additional gene control elements, where at least the first two are linked and the sequence being expressed is heterologous with respect to EU; (6) expression vector (EV) that contains EC; (7) genetically modified microorganisms (GMO) having, for at least one gene, an altered (or induced) transcription rate, relative to the wild type; (8) preparing biosynthetic products by culturing GMO of (7); (9) use of the sequence ggaggga (53) as a ribosome-binding site in expression units that provide expression of genes in Corynebacterium or Brevibacterium; (10) use of the sequence tagagt (52) as a -10 region for expression of genes in Corynebacterium or Brevibacterium; and (11) expression units that contain sequences (52) or (53).

公开(公告)号：DE10359594A1

公开(公告)日：2005-07-28

申请号：DE10359594

申请日：2003-12-18

Applicant: BASF AG

Inventor： KROEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

IPC: C07K14/34 ,  C12N20060101 ,  C12N1/00 ,  C12N15/63 ,  C12N15/67 ,  C12N15/77 ,  C12P1/00 ,  C12P13/08 ,  C12P13/12

Abstract: Use, for transcribing genes, of a nucleic acid (I) with promoter activity, where (I) is a 186 base pair sequence (1), reproduced; a variant of (1) with at least 90% identity and derived by substitution, insertion or deletion of nucleotides; a sequence that hybridizes to (1) under stringent conditions; or a functionally equivalent fragment of them, is new. Independent claims are also included for the following: (1) use of an expression unit (EU), containing (I) and functionally linked to a sequence (X) that ensures translation of RNA, for expressing genes; (2) (I), other than sequence (1), i.e. (Ia), as new compounds; (3) EU that contain (Ia) linked to (X); (4) altering (or producing) the transcription rate of genes in a microorganism, relative to the wild type; (5) expression cassette (EC) comprising at least one EU of (1), at least one other nucleic acid sequence (to be expressed) and optionally additional gene control elements, where at least the first two are linked and the sequence being expressed is heterologous with respect to EU; (6) expression vector (EV) that contains EC; (7) genetically modified microorganisms (GMO) having, for at least one gene, an altered (or induced) transcription rate, relative to the wild type; (8) preparing biosynthetic products by culturing GMO of (7); (9) use of the sequence aggagga (42) as a ribosome-binding site in expression units that provide expression of genes in Corynebacterium or Brevibacterium; (10) use of the sequences tagttt (39), taggat (40) or tgcgct (41) as -10 regions for expression of genes in Corynebacterium or Brevibacterium; and (11) expression units that contain sequences (39)-(42).

公开(公告)号：CA2548964A1

公开(公告)日：2005-06-30

申请号：CA2548964

申请日：2004-12-17

Applicant: BASF AG

Inventor： SCHROEDER HARTWIG ,  KLEIN DANIELA ,  BOY MATTHIAS

IPC: C12P13/12 ,  A23L1/305 ,  C07C319/28 ,  C07C323/58

Abstract: The invention relates to a method for the fermentative production of methionine, to a method for the insolation of the methionine thus formed, organic substances containing methionine occurring during insulation, to the use thereof in the production of animal food or animal food complements, in addition to the use of insolated methionine in the production of human or animal food or human or animal food complements.