公开(公告)号：WO2017218789A1

公开(公告)日：2017-12-21

申请号：PCT/US2017/037700

申请日：2017-06-15

Applicant: STRECK, INC.

Inventor： TORRES, Maria ,  HANSON, Nancy ,  LECHNER, Joel ,  COSSETTE, Stephanie ,  SCHEER, Cathy ,  KREIFELS, Matthew ,  MORROW, Stacey ,  CONNELLY, Chris ,  PORTER, Laura, R. ,  FOWLER, Randy

IPC: C12Q1/68

Abstract: Assays and methods for detecting resistance to beta-lactam antibiotics including detection of multiple β-lactamase family specific gene targets by polymerase chain reaction or microarray. One or more kits including primers and/or probes for identification of β-lactamase genes selected from the group consisting of one or more of the following:MOX-like, FOX-like, ACC-like, ACT/MIR-like, CMY-2-like, DHA-like, CTX-M-14-like, CTX-M-15-like, VIM-like, NDM-like, IMP-like, KPC-like, and OXA-48-like, OXA-51 -like, OXA-143-like, OXA-58-like, OXA-23-like, OXA-24/40-like, TEM-like, and SHV-like. A kit may also include one or more primers and/or probes for the identification a non-beta lactamase gene family which confers antibiotic resistance, such as the MCR-1 gene.

Abstract translation: 用于检测对β-内酰胺抗生素的抗性的分析方法和方法，包括通过聚合酶链式反应或微阵列检测多种β-内酰胺酶家族特异性基因靶标。 一种或多种试剂盒，包括用于鉴定选自以下一种或多种的β-内酰胺酶基因的引物和/或探针：MOX样，FOX样，ACC样，ACT / MIR样，CMY样， 2样，DHA样，CTX-M-14样，CTX-M-15样，VIM样，NDM样，IMP样，KPC样和OXA-48样，OXA-51 如OXA-143样，OXA-58样，OXA-23样，OXA-24/40样，TEM样和SHV样。 试剂盒还可以包括用于鉴定赋予抗生素抗性的非-β内酰胺酶基因家族的一个或多个引物和/或探针，例如MCR-1基因。

公开(公告)号：WO2011057184A1

公开(公告)日：2011-05-12

申请号：PCT/US2010/055815

申请日：2010-11-08

Applicant: STRECK, INC. ,  RYAN, Wayne, L. ,  FERNANDO, M., Rohan

Inventor： RYAN, Wayne, L. ,  FERNANDO, M., Rohan

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12N15/1003 ,  C12Q1/6806 ,  C12Q2527/127 ,  C12Q2527/137 ,  C12Q2527/101

Abstract: A method for preserving and processing nucleic acids located within a blood sample is disclosed, wherein a blood sample containing nucleic acids is treated to reduce both blood cell lysis and nuclease activity within the blood sample. The treatment of the sample aids in increasing the integrity and amount of cellular nucleic acids that can be identified and tested while avoiding contamination of the isolated nucleic acids with cell -free nucleic acids. The preferred compositions for preserving and processing contain diazolidinyl urea (DU), EDTA, aurintricarboxylic acid (ATA), glyceraldehyde and sodium floride.

Abstract translation: 公开了一种用于保存和处理位于血液样品内的核酸的方法，其中处理含有核酸的血液样品以降低血液样品中的血细胞裂解和核酸酶活性。 样品的处理有助于增加可以鉴定和测试的细胞核酸的完整性和量，同时避免用无细胞核酸污染分离的核酸。 用于保存和加工的优选组合物含有二唑烷基脲（DU），EDTA，三羟甲基（ATA），甘油醛和氟化钠。

公开(公告)号：WO2022036174A1

公开(公告)日：2022-02-17

申请号：PCT/US2021/045874

申请日：2021-08-13

Applicant: STRECK, INC.

Inventor： SALAMIFAR, Sean ,  GEORGE, Nick ,  HUNSLEY, Brad

IPC: B01L3/00 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/16 ,  B01L3/5082

Abstract: The present teachings relate to a method of making a biological sample collection container, an internally coated biological sample collection container, and uses of the same, particularly for omic analysis. A reagent (or a reagent precursor) is deposited in a liquid state at least partially along at least one side wall of the container. The reagent precursor is dried to form a dried coating having a predefined pattern and topology along at least a portion of the at least one side wall. A container thus results having a coating that includes, in a dried state, a stabilizer agent, or reaction product of a stabilizer agent and an anticoagulant, and upon collection of a sample enables stabilization of any present white blood cells, cell-free nucleic acids, extracellular vesicles, circulating tumor cells, proteins, metabolites, lipids, or any combination thereof, and preserving them in sufficient quantity and quality for omic analysis.

公开(公告)号：WO2015134053A1

公开(公告)日：2015-09-11

申请号：PCT/US2014/044867

申请日：2014-06-30

Applicant: STRECK, INC.

Inventor： KREIFELS, Matthew, R. ,  WHITNEY, Scott, E. ,  DOWLING, James ,  JUST, Troy

IPC: B01L3/00 ,  G01N21/03

CPC classification number: B01L3/5082 ,  B01L7/52 ,  B01L2300/021 ,  B01L2300/022 ,  B01L2300/043 ,  B01L2300/0654 ,  B01L2300/0832 ,  B01L2300/0848 ,  B01L2300/0851 ,  B01L2300/123 ,  B01L2300/168 ,  C12Q1/686 ,  G01N21/03 ,  G01N2021/0364 ,  G01N2021/0382 ,  G01N2021/6482 ,  G01N2021/6484

Abstract: An improved sample tube that includes a body portion having a longitudinal axis and a wall generally circumscribing the longitudinal axis. The body portion terminates in a distal tip having a dimple. The body portion includes at least one transparent portion (e.g., at the distal tip) that is adapted for transmitting light. The wail is configured for elastic deformation along at feast a portion of its length, including in a direction that is generally transverse to the longitudinal axis, so that it compressively and resiliently deforms and engages a wail defining an opening in a sample block of a PGR amplification instrument. The tube may be made by injection molding a polymeric material including a thermoplastic.

Abstract translation: 一种改进的样品管，其包括具有纵向轴线的主体部分和通常围绕纵向轴线的壁。 主体部分终止于具有凹坑的远端。 主体部分包括适于透射光的至少一个透明部分（例如，在远端）。 围栏被构造成沿着其长度的一部分包括在大致横向于纵向轴线的方向上的弹性变形，使得其压缩和弹性变形并且接合限定PGR的样品块中的开口的壁 放大仪器。 管可以通过注射模制包括热塑性塑料的聚合材料制成。

公开(公告)号：WO2015013244A1

公开(公告)日：2015-01-29

申请号：PCT/US2014/047551

申请日：2014-07-22

Applicant: STRECK, INC.

Inventor： FERNANDO, M., Rohan ,  RYAN, Wayne, L. ,  HUNSLEY, Brad

IPC: A01N1/02 ,  G01N33/574

CPC classification number: A01N1/0226 ,  G01N33/574

Abstract: Compositions and Methods for Stabilizing Circulating Tumor Cells Methods and compositions for stabilizing a biological sample for analysis, comprising the steps of obtaining in a sample collection device a biological sample from a subject, especially blood, the biological sample including at least one circulating tumor cell from the subject. The methods may include a step of contacting the biological sample with a protective agent composition that includes a preservative agent, an optional anticoagulant, and a quenching agent to form a mixture that includes the protective agent composition and the sample.

Abstract translation: 用于稳定循环肿瘤细胞的组合物和方法用于稳定生物样品用于分析的方法和组合物，包括以下步骤：在样品收集装置中获得来自受试者，特别是血液的生物样品，所述生物样品包括至少一种循环肿瘤细胞， 主题。 所述方法可以包括使生物样品与包含防腐剂，任选的抗凝血剂和猝灭剂的保护剂组合物接触以形成包含保护剂组合物和样品的混合物的步骤。

公开(公告)号：WO2013123030A2

公开(公告)日：2013-08-22

申请号：PCT/US2013/025912

申请日：2013-02-13

Applicant: STRECK, INC.

Inventor： RYAN, Wayne, L. ,  FERNANDO, M., Rohan ,  DAS, Kausik

IPC: C12Q1/68

CPC classification number: C12N15/1003 ,  C12Q1/6806 ,  C12Q2527/125

Abstract: Methods and devices for stabilizing a biological sample for analysis, comprising the steps of obtaining in a sample collection device a biological sample from a subject, the biological sample including at (east one circulating cell-free first nucleic acid from the subject. The methods may include a step of contacting the.biological sample while within the sample collection device with a protective agent composition that Includes a preservative agent, an optionai anticoagulant, and a quenching agent to form a mature that: includes the protective agent composition and the sample.

Abstract translation: 用于稳定生物样品用于分析的方法和装置，包括以下步骤：在样品收集装置中获得来自受试者的生物样品，所述生物样品包括（东侧一个来自受试者的无循环的无细胞的第一核酸），所述方法可 包括在样品收集装置内与具有保护剂组合物的生物样品接触的步骤，所述保护剂组合物包括防腐剂，选择性抗凝血剂和猝灭剂，以形成包含保护剂组合物和样品的成熟剂。

公开(公告)号：WO2013019290A2

公开(公告)日：2013-02-07

申请号：PCT/US2012/034434

申请日：2012-04-20

Applicant: STRECK, INC. ,  RYAN, Wayne, L. ,  SCHOLL, John, W.

Inventor： RYAN, Wayne, L. ,  SCHOLL, John, W.

IPC: C12N5/078

CPC classification number: G01N33/96

Abstract: A composition (and associated methods) including a plurality of treated red blood cells for simulating reticulocytes, and particularly an immature reticulocyte fraction, of whole blood when processed as a sample in an automated analyzer capable of detecting reticulocytes. A method for making the composition or other simulated reticuolcyte may include steps of contacting a suspension of a plurality of red blood cells each having a membrane in an initial state that surrounds an interior volume of a cell with an effective amount of a hypertonic permeabilizing solution including dimethyl sulfoxide and a hypotonic loading agent delivery solution including a loading agent, for a sufficient time to form a plurality of pores in the membrane, for permitting the loading agent to enter into the interior volume of the cells, and, after entry of a desired amount of the loading agent into the interior volume of the cell, for sealing the pores for substantially restoring the membrane to the initial state while substantially encapsulating the loading agent within the resulting cell, and preferably also retaining within the resulting cell a substantial amount of hemoglobin from the original starting red blood cell.

Abstract translation: 当在自动分析仪中作为样品处理时，包括多个用于模拟网织红细胞特别是未成熟网织红细胞部分的处理过的红细胞的组合物（和相关方法），所述自动分析仪能够检测网织红细胞 。 用于制备组合物或其他模拟网状细胞的方法可以包括以下步骤：使多种红细胞的悬浮液与包含细胞内部体积的初始状态下的膜接触，所述初始状态包含有效量的高渗透性溶液，所述高渗透性溶液包括 二甲基亚砜和包括负载剂的低渗负载剂递送溶液混合足够的时间以在膜中形成多个孔，用于允许负载剂进入细胞的内部体积中，并且在输入期望的 将填充剂的量加入细胞的内部容积中，用于密封孔隙以使膜基本上恢复至初始状态，同时将所述加载剂基本上包封在所得细胞内，并且优选还在所得细胞内保留大量血红蛋白 从最初的红血球开始。

公开(公告)号：WO2012151391A3

公开(公告)日：2013-01-03

申请号：PCT/US2012036304

申请日：2012-05-03

Applicant: STRECK INC ,  RYAN WAYNE L ,  GRUNKEMEYER JAMES A

Inventor： RYAN WAYNE L ,  GRUNKEMEYER JAMES A

IPC: A61K39/00 ,  A61K39/145 ,  C12N7/06

CPC classification number: A61K39/275 ,  A61K39/12 ,  A61K2039/5252 ,  C12N7/00 ,  C12N2760/16134 ,  C12N2760/16163

Abstract: The present invention is directed at a composition comprising a live swine flu virus having an infectious component and a plurality of surface antigens in contact with a formaldehyde donor agent having a molecular weight that is less than about 400 g/mol. Preferably, the formaldehyde donor agent is selected from a non-crosslinking chemical fixative that contains urea.

Abstract translation: 本发明涉及包含具有感染性成分的活猪流感病毒和与分子量小于约400g / mol的甲醛供体试剂接触的多种表面抗原的组合物。 优选地，甲醛供体剂选自含有尿素的非交联化学固定剂。

公开(公告)号：WO2012166913A1

公开(公告)日：2012-12-06

申请号：PCT/US2012/040201

申请日：2012-05-31

Applicant: STRECK, INC. ,  TERMAAT, Joel, R. ,  WHITNEY, Scott, E. ,  VILJOEN, Hendrik, J. ,  KREIFELS, Matthew, R.

Inventor： TERMAAT, Joel, R. ,  WHITNEY, Scott, E. ,  VILJOEN, Hendrik, J. ,  KREIFELS, Matthew, R.

IPC: C12Q1/68 ,  G01N35/00 ,  B01L7/00

CPC classification number: B01L7/52 ,  B01L2200/14 ,  B01L2300/0627 ,  B01L2300/0858 ,  B01L2300/1805 ,  B01L2300/1822 ,  C12Q1/6844 ,  G01N2035/00366

Abstract: A thermocycling device (10) and method of operating in which at least one first temperature sensor (1060) monitors a first temperature condition within the sample holder (64) and is adapted to provide a first electrical signal corresponding with the first temperature condition. In addition, at least one second temperature sensor (87) monitors a second temperature condition of a component external of the sample holder and is adapted to provide a second signal corresponding with the second temperature condition. At least one control device is adapted to interface with a power supply (1010) to control the delivery of power to a first thermal cycling element (60) and a second thermal cycling element (62), (both of which may sandwich a sample holder) and optionally an air mover (54); adapted to be in signaling communication with the first temperature sensor and the second temperature sensor; is configured for analysis of signals from the first temperature sensor and the second temperature sensor and; is configured such that, based upon such analysis, the at least one control device alters the power that is delivered to one or more of the first thermal cycling element and second thermal cycling element, and/or the air mover. The device Is controllably operated to account for thermal inertia effects.

Abstract translation: 一种热循环装置（10）和操作方法，其中至少一个第一温度传感器（1060）监测样品保持器（64）内的第一温度条件，并适于提供对应于第一温度条件的第一电信号。 此外，至少一个第二温度传感器（87）监测样品保持器外部的部件的第二温度条件，并且适于提供对应于第二温度条件的第二信号。 至少一个控制装置适于与电源（1010）接口，以控制向第一热循环元件（60）和第二热循环元件（62）的输送，（两者都可以夹持样品架 ）和任选的鼓风机（54）; 适于与第一温度传感器和第二温度传感器进行信令通信; 被配置用于分析来自第一温度传感器和第二温度传感器的信号; 被配置为使得基于这种分析，所述至少一个控制装置改变被输送到第一热循环元件和第二热循环元件和/或鼓风机中的一个或多个的功率。 该装置可控地操作以考虑热惯性效应。

公开(公告)号：CA2938275C

公开(公告)日：2021-08-31

申请号：CA2938275

申请日：2010-01-21

Applicant: STRECK INC

Inventor： FERNANDO M ROHAN ,  CHAO-WEI CHEN KATE

IPC: C12N15/10 ,  G01N33/48

Abstract: Compositions for preserving and processing fetal nucleic acids located within maternal blood samples are disclosed, wherein a sample of maternal blood containing fetal nucleic acids is treated with a composition to reduce both cell lysis of the maternal blood cells and deoxyribonuclease (DNase) and ribonuclease (RNase) activity within the fetal nucleic acids. Treatment of the sample with a composition as disclosed herein aids in increasing the arnount of fetal nucleic acids that can be identified and tested while maintaining the structure and integrity of the fetal nucleic acids.