公开(公告)号：KR100790801B1

公开(公告)日：2008-01-04

申请号：KR1020050060920

申请日：2005-07-06

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 성환우 ,  이윤정 ,  최준구 ,  이은경 ,  권준헌 ,  김재홍

IPC: C12N7/01 ,  C12N7/04

Abstract: 본 발명은 HA 단백질 분절부위 아미노산 배열의 모티프가 TSGR을 가지며, 국내유행 저병원성 조류인플루엔자 바이러스에 방어능력을 나타내는 백신제조용 H9N2 혈청형의 저병원성 조류인플루엔자 바이러스주를 제공한다. 상기 바이러스주를 백신제조용 원료로 사용하는 경우 양계산업에 있어 산란율 저하 및 폐사 등 지속적인 문제를 일으키는 H9N2 혈청형 저병원성 조류인플루엔자 예방에 활용이 가능하다.
저병원성 조류인플루엔자, H9N2, 백신

公开(公告)号：KR1020060132155A

公开(公告)日：2006-12-21

申请号：KR1020050052325

申请日：2005-06-17

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 성환우 ,  이윤정 ,  최준구 ,  이은경 ,  김재홍

IPC: A61K39/145 ,  A61K39/12

CPC classification number: A61K39/145 ,  A61K38/02 ,  Y10S514/888

Abstract: A low pathogenic avian influenza viral vaccine for preventing H5 subtype avian influenza viral infection is provided to enhance safety and medicinal efficacy of use, and prevent the infection of H5N1 type high pathogenic avian influenza virus safely. The method for preparing the low pathogenic avian influenza viral vaccine for preventing H5 subtype avian influenza viral infection comprises the steps of: (1) culturing a A/wild bird feces/Korea/CSM-2/02(H5N3) strain in a specific pathogen free egg(SPF egg) for 72 hours and collecting allantoic fluid; (2) measuring the titrate of the virus collected in step(1) through hemagglutination inhibition test; (3) concentrating the virus 10 times by centrifuging the virus at 18,000 rpm for 3 hours; (4) attenuating the virus by treating the concentrated virus with 0.1% formalin at 20 deg.C for 12 hours; and (5) mixing the attenuated virus solution with oil adjuvant ISA70 in a weight ratio of 3:7, wherein the low pathogenic avian influenza viral vaccine contains at least one protein encoded by the gene of SEQ ID NO:2 to SEQ ID NO:9.

Abstract translation: 提供用于预防H5亚型禽流感病毒感染的低致病性禽流感病毒疫苗，以提高使用的安全性和药用效力，并安全防止H5N1型高致病性禽流感病毒感染。 制备用于预防H5亚型禽流感病毒感染的低致病性禽流感病毒疫苗的方法包括以下步骤：（1）在特定病原体中培养A /野鸟粪/韩国/ CSM-2/02（H5N3）菌株 免费鸡蛋（SPF蛋）72小时并收集尿囊液; （2）通过血细胞凝集抑制试验测定步骤（1）中收集的病毒的滴度; （3）通过以18,000rpm将病毒离心3小时来浓缩病毒10次; （4）通过用0.1％福尔马林在20℃处理浓缩的病毒12小时来减毒病毒; 和（5）将减毒病毒溶液与油佐剂ISA70以3:7的重量比混合，其中低致病性禽流感病毒疫苗含有由SEQ ID NO：2至SEQ ID NO：2的基因编码的至少一种蛋白质。 9。

公开(公告)号：KR101463004B1

公开(公告)日：2014-12-04

申请号：KR1020120151046

申请日：2012-12-21

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 최강석 ,  계수정 ,  김지예 ,  권준헌 ,  이희수 ,  성환우

IPC: C12N15/63 ,  C12N15/45 ,  C12N15/85 ,  G01N33/68

Abstract: 본발명은베큘러바이러스에의하여발현된조류파라믹소바이러스-9 재조합 HN 단백질및 이를이용한조류파라믹소바이러스-9 진단방법에관한것이다. 본발명에따른 APMV-9 HN 단백질을발현하는베큘러바이러스또는이에의하여형질감염된곤충세포는 APMV-9 HN 단백질을고농도로생산하며, 이를이용하여세포배양이가능한일반실험실에서도손쉽게대량의항원을제조할수 있고, 곤충세포배양법으로일주일이내에항원진단액제조가가능하므로항원진단액제조기간을최소일주일이상단축할수 있는효과가있다. 또한, 본발명에의해제조한 APMV-9 HN 단백질항원은닭 적혈구에대한혈구응집능과열 안정성이있고, APMV-9 면역혈청에의해 HI 반응이특이적으로나타났을뿐만아니라, 종래의항원 (APMV-9 바이러스항원)과 HI반응법검사결과와비교하였을때 동일한검사결과를나타내는효과가있다. 따라서, 감염성 APMV-9 바이러스를확보하고있지않아도종래의 APMV-9 바이러스항원진단액을본 발명에의해제조한 APMV-9 HN 단백질항원으로대체하여사용할수 있다.

公开(公告)号：KR101462998B1

公开(公告)日：2014-11-19

申请号：KR1020120150960

申请日：2012-12-21

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 최강석 ,  계수정 ,  김지예 ,  권준헌 ,  이희수 ,  성환우

IPC: C12N15/63 ,  C12N15/45 ,  C12N15/85 ,  G01N33/68

Abstract: 본 발명은 제2형 조류파라믹소바이러스 (avian paramyxovirus-2, APMV-2)의 헤마글루티닌-뉴라미니다아제 (hemagglutinin-neuraminidase, HN) 단백질을 코딩하는 유전자를 포함하는 재조합 바이러스 발현 벡터, 상기 벡터에 의해 형질전환된 제2형 조류파라믹소바이러스의 헤마글루티닌-뉴라미니다아제 단백질을 발현하는 재조합 곤충 세포, 상기 재조합 곤충세포가 발현하는 제2형 조류파라믹소바이러스의 헤마글루티닌-뉴라미니다아제 재조합 항원 단백질을 포함하는 제2형 조류파라믹소바이러스 진단용 조성물, 진단 키트 및 이를 이용한 진단 방법에 관한 것이다. 본 발명에 따른 진단용 조성물은 살아있는 바이러스 취급에 따른 오염 가능성 없이 안전하고, 신속하게 대량의 샘플로부터 제2형 조류파라믹소바이러스의 감염 여부를 정확하게 진단할 수 있는 우수한 효과를 가지고 있다.

公开(公告)号：KR1020010095727A

公开(公告)日：2001-11-07

申请号：KR1020000019033

申请日：2000-04-11

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 송창선 ,  이윤정 ,  성환우 ,  한명국 ,  김기석

IPC: C12N15/63

CPC classification number: C12N15/63 ,  A61K39/12 ,  C12N7/00

Abstract: PURPOSE: A recombinant Marek's disease virus type 2(MDV2) expressing the S1 glycoprotein of infectious bronchitis virus(IBV) and its construction methods are provided, to effectively use in preventing the infectious bronchitis of chicken. CONSTITUTION: The Marek's disease virus type 2(MDV2) expression vector pKGFKM contains the S1 glycoprotein gene of infectious bronchitis virus(IBV). The recombinant Marek's disease virus type 2(MDV2) expressing the S1 glycoprotein of infectious bronchitis virus(IBV) rMDV2-US3S1 (KFCC 11154) is prepared by transforming the expression vector pKGFKM. The recombinant Marek's disease virus type 2(MDV2) expressing the S1 glycoprotein of infectious bronchitis virus(IBV) is constructed by co-transfecting Marek's disease virus type 2(MDV2) expression vector pKGFKM containing the S1 glycoprotein gene of infectious bronchitis virus(IBV) and genomic MDV HPRS24 DNA into the CEF cell by using electroporation method.

Abstract translation: 目的：提供表达感染性支气管炎病毒（IBV）S1糖蛋白的重组马立克氏病病毒2型（MDV2）及其构建方法，有效用于预防鸡传染性支气管炎。 构成：马立克氏病病毒2型（MDV2）表达载体pKGFKM含有感染性支气管炎病毒（IBV）的S1糖蛋白基因。 通过转化表达载体pKGFKM制备表达感染性支气管炎病毒（IBV）rMDV2-US3S1（KFCC 11154）的S1糖蛋白的重组马立克氏病病毒2型（MDV2）。 通过共转染含有感染性支气管炎病毒（IBV）的S1糖蛋白基因的马立克氏病毒2型（MDV2）表达载体pKGFKM构建表达感染性支气管炎病毒（IBV）的S1糖蛋白的重组马立克氏病病毒2型（MDV2） 和基因组MDV HPRS24 DNA通过电穿孔法进入CEF细胞。

公开(公告)号：KR101462985B1

公开(公告)日：2014-11-18

申请号：KR1020120150976

申请日：2012-12-21

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 최강석 ,  계수정 ,  김지예 ,  권준헌 ,  이희수 ,  성환우

IPC: C12N15/63 ,  C12N15/45 ,  C12N15/85 ,  G01N33/68

Abstract: 본 발명은 제3형 조류파라믹소바이러스 (avian paramyxovirus-3, APMV-3)의 헤마글루티닌-뉴라미니다아제 (hemagglutinin-neuraminidase, HN) 단백질을 코딩하는 유전자를 포함하는 재조합 바이러스 발현 벡터, 상기 벡터에 의해 형질전환된 제3형 조류파라믹소바이러스의 헤마글루티닌-뉴라미니다아제 단백질을 발현하는 재조합 곤충 세포, 상기 재조합 곤충세포가 발현하는 제3형 조류파라믹소바이러스의 헤마글루티닌-뉴라미니다아제 재조합 항원 단백질을 포함하는 제3형 조류파라믹소바이러스 진단용 조성물, 진단 키트 및 이를 이용한 진단 방법에 관한 것이다. 본 발명에 따른 진단용 조성물은 살아있는 바이러스 취급에 따른 오염 가능성 없이 안전하고, 신속하게 대량의 샘플로부터 제3형 조류파라믹소바이러스의 감염 여부를 정확하게 진단할 수 있는 우수한 효과를 가지고 있다.

公开(公告)号：KR1020140081332A

公开(公告)日：2014-07-01

申请号：KR1020120150960

申请日：2012-12-21

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 최강석 ,  계수정 ,  김지예 ,  권준헌 ,  이희수 ,  성환우

IPC: C12N15/63 ,  C12N15/45 ,  C12N15/85 ,  G01N33/68

Abstract: The present invention relates to a recombinant virus expression vector including a gene which encodes hemagglutinin-neuraminidase (HN) protein of avian paramyxovirus-2 (APMV-2); to recombinant insect cells transformed by the vector and expressing the HN protein of APMV-2; and to a diagnostic composition for APMV-2 including the recombinant antigenic HN protein of APMV-2 expressed by the recombinant insect cells, a diagnostic kit including the HN protein, and a diagnostic method using the HN protein. The diagnostic composition for APMV-2 according to the present invention is safe without the possibility of contamination caused by treatment of live viruses and has an excellent effect of quickly and accurately diagnosing whether there is infection with APMV-2 from a large number of samples.

Abstract translation: 本发明涉及包含编码禽副粘病毒-2（APMV-2）的血凝素 - 神经氨酸酶（HN）蛋白的基因的重组病毒表达载体; 通过载体转化并表达APMV-2的HN蛋白的重组昆虫细胞; 以及包含由重组昆虫细胞表达的APMV-2的重组抗原性HN蛋白质的APMV-2诊断组合物，包含HN蛋白质的诊断试剂盒和使用HN蛋白质的诊断方法。 根据本发明的APMV-2的诊断组合物是安全的，没有由病毒治疗引起的污染的可能性，并且具有快速且准确地诊断来自大量样品是否存在APMV-2感染的优异效果。

公开(公告)号：KR1020140081287A

公开(公告)日：2014-07-01

申请号：KR1020120150870

申请日：2012-12-21

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 최강석 ,  계수정 ,  김지예 ,  권준헌 ,  이희수 ,  성환우

IPC: C12N15/63 ,  C12N15/45 ,  C12N15/85 ,  C07K14/08

Abstract: The present invention relates to avian paramyxovirus (APMV)-7 recombinant hemagglutinin-neuraminidase (HN) protein expressed by baculoviruses, and a diagnostic method of APMV-7 using the same. The baculoviruses expressing APMV-7 HN protein according to the present invention, or insect cells transfected with the baculoviruses produce a high concentration of APMV-7 HN protein; easily enable the production of a large number of antigens even in a general laboratory allowing cell culture by using the APMV-7 HN protein; and enable the production of an antigen diagnostic reagent within a week by an insect cell culture method, thereby having an effect of shortening the production period of the antigen diagnostic reagent by at least one week. In addition, an APMV-7 HN protein antigen produced by the present invention has hemagglutination ability for erythrocytes of a chicken and thermal stability; has exhibited a specific HI reaction by APMV-7 immune serum; and also has an effect of exhibiting the same test result in comparison with a HI reaction test result from a conventional antigen (APMV-7 virus antigen). Therefore, a conventional APMV-7 virus antigen diagnostic reagent can be replaced with the APMV-7 HN protein antigen produced by the present invention even if infectious APMV-7 viruses are not secured.

Abstract translation: 本发明涉及由杆状病毒表达的禽副粘病毒（APMV）-7重组血凝素 - 神经氨酸酶（HN）蛋白，以及使用其的APMV-7的诊断方法。 表达本发明的APMV-7HH蛋白的杆状病毒或用杆状病毒转染的昆虫细胞产生高浓度的APMV-7HH蛋白; 即使在通常使用APMV-7HH蛋白的细胞培养的实验室中也容易产生大量抗原; 并且能够通过昆虫细胞培养法在一周内生产抗原诊断试剂，从而具有将抗原诊断试剂的生产周期缩短至少一周的效果。 此外，本发明生产的APMV-7HH蛋白抗原具有鸡的红细胞的血细胞凝集能力和热稳定性; 已经通过APMV-7免疫血清显示出特异的HI反应; 并且与常规抗原（APMV-7病毒抗原）的HI反应试验结果相比，具有显示相同试验结果的效果。 因此，即使不能确保感染性APMV-7病毒，也可以用本发明生产的APMV-7HH蛋白抗原代替传统的APMV-7病毒抗原诊断试剂。

公开(公告)号：KR1020070072945A

公开(公告)日：2007-07-10

申请号：KR1020060000325

申请日：2006-01-03

Applicant: 대한민국(농림축산식품부 농림축산검역본부장) ,  주식회사 바이오노트

Inventor： 이윤정 ,  최준구 ,  이은경 ,  권용국 ,  권준헌 ,  김재홍 ,  성환우 ,  오진식 ,  조영식 ,  조병기

IPC: G01N33/53

Abstract: A diagnostic strip for a H5 type highly pathogenic avian influenza and general avian influenza is provided to conveniently and rapidly diagnose whether a bird is infected by the H5 type highly pathogenic avian influenza and general avian influenza or not from stool in situ without special testing equipment. The diagnostic strip is characterized in that a monoclonal antibody common to general avian influenza virus in which hemaglutinin antigens are H1 to H15 and a monoclonal antibody specific to a highly pathogenic avian influenza in which a hemaglutinin antigen is H5 are used for diagnosing the highly pathogenic and the general avian influenza in which the hemaglutinin antigen is H5 from stool or tissue of chicken. The method for preparing the diagnostic strip comprises the steps of: (a) respectively absorbing the monoclonal antibody specific to the highly pathogenic avian influenza virus in which the hemaglutinin antigen is H5 and the monoclonal antibody common to general avian influenza virus into a membrane; (b) respectively attaching the antibody absorbed membrane obtained from the step(a) to gold particles so as to prepare an antibody-gold conjugate and then mixing it; and (c) precipitating the conjugate obtained from the step(b) in a basic material and then drying it.

Abstract translation: 提供一种H5型高致病性禽流感和一般禽流感的诊断条，以方便快捷地诊断鸟类是否由H5型高致病性禽流感和一般禽流感感染，或者不是在没有特殊检测设备的情况下从粪便中感染。 诊断条的特征在于，其中血凝素抗原为H1至H15的一般性禽流感病毒共有的单克隆抗体和其中血凝素抗原为H5的高致病性禽流感特异性的单克隆抗体用于诊断高致病性和 其中血凝素抗原为粪便或鸡组织中H5的一般性禽流感。 制备诊断条的方法包括以下步骤：（a）分别将其中血凝素抗原为H5的高致病性禽流感病毒和一般禽流感病毒共有的单克隆抗体特异性的单克隆抗体吸收到膜中; （b）将由步骤（a）获得的抗体吸收膜分别附着于金颗粒上，制备抗体 - 金缀合物，然后混合; 和（c）将由步骤（b）获得的缀合物沉淀在碱性材料中，然后干燥。

公开(公告)号：KR1020070005867A

公开(公告)日：2007-01-10

申请号：KR1020050060920

申请日：2005-07-06

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 성환우 ,  이윤정 ,  최준구 ,  이은경 ,  권준헌 ,  김재홍

IPC: C12N7/01 ,  C12N7/04

Abstract: A virus strain for preparing a vaccine is provided to have excellent defending effect on low pathogenic avian influenza H9N2 virus strain. And a vaccine is provided to be able to effectively prevent diseases caused by the low pathogenic avian influenza H9N2 recording the high mortality of bird. The low pathogenic avian influenza H9N2 virus strain for preparing vaccine is characterized in that a motif of hemangioblast protein cleavage site amino acid sequence has TSGR and it shows the defending capacity against domestic low pathogenic avian influenza virus. The method for preparing a vaccine for the low pathogenic avian influenza H9N2 virus strain comprises the steps of: (a) inactivating the low pathogenic avian influenza H9N2 virus strain; and (b) mixing the inactivated virus with an adequate excipient.

Abstract translation: 提供用于制备疫苗的病毒株，以对低致病性禽流感H9N2病毒株具有优异的防御作用。 并提供疫苗能够有效预防由低致病性禽流感H9N2引起的疾病，记录鸟类的高死亡率。 用于制备疫苗的低致病性禽流感H9N2病毒株的特征在于成血管细胞蛋白切割位点氨基酸序列的基序具有TSGR，并且显示出针对国内低致病性禽流感病毒的防御能力。 用于制备低致病性禽流感H9N2病毒株的疫苗的方法包括以下步骤：（a）灭活低致病性禽流感H9N2病毒株; 和（b）将灭活的病毒与足够的赋形剂混合。