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公开(公告)号：KR101557191B1
公开(公告)日：2015-10-02
申请号：KR1020130075529
申请日：2013-06-28
Applicant: 조지아 스테이트 유니버시티 리서치 파운데이션, 인코포레이티드 , 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C07K14/11 , C12N7/01 , A61K39/145 , A61P31/16
Abstract: 본발명은넓은범위의방어능력을갖는인플루엔자단독백신또는이를이용한인플루엔자보강백신에관한것이다. 본발명의인플루엔자단독백신또는인플루엔자보강백신은다양한혈청형의인플루엔자바이러스감염에대하여 M2e단백질에대한면역력을증가시킴으로써전국적유행병과같이예측하지못한바이러스의확산에효과적으로대처할수 있는광범위한방어능력을가진다.
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公开(公告)号：KR101415836B1
公开(公告)日：2014-07-09
申请号：KR1020120076917
申请日：2012-07-13
Applicant: 서울대학교산학협력단 , 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N7/01 , C12N15/63 , C12N15/33 , A61K39/145
Abstract: 조류 인플루엔자 바이러스의 HA 단백질 유래의 HA2 절편의 154번째 아미노산인 아스파라긴(N)에 N-글리칸 생성이 일어나지 않도록 154번째 아미노산인 아스파라긴 또는 156번째 아미노산인 세린(S)이나 트레오닌(T)을 다른 아미노산으로 치환하여 HA2 공통 에피톱에 대한 면역원성을 증가시키는 방법과 이러한 방법을 통해 면역원성이 증가된 재조합 바이러스가 제공된다. 본 발명의 HA2 공통 에피톱 면역원성 향상기술과 HA2 공통 에피톱 고면역원성 바이러스는 인플루엔자 예방을 위한 백신주 개발과 백신주로써 유용하게 이용될 수 있다.
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3.发明授权신속 면역크로마토그라피법에 의한 Ｈ9형 조류인플루엔자 바이러스 진단 키트 및 이를 이용하여 Ｈ9형 조류인플루엔자를 진단하는 방법 有权使用快速免疫层析技术的H9型禽流感病毒诊断试剂盒及使用相同方法诊断H9型禽流感
公开(公告)号：KR101211593B1
公开(公告)日：2013-01-07
申请号：KR1020100087163
申请日：2010-09-06
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , 주식회사 바이오노트
IPC: G01N33/569 , G01N33/538 , G01N33/553 , C07K16/08
Abstract: 본발명은헤마글루티닌항원이 H9형조류인플루엔자에특이적인항체를이용한신속면역크로마토그라피법에의해닭 총배설강(분변) 스왑시료에서 H9형조류인플루엔자감염여부를신속하고정확하게진단할수 있는진단키트및 이를이용하여 H9형조류인플루엔자를진단하는방법에관한것이다.
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4.发明公开신속 면역크로마토그라피법에 의한 Ｈ5형 고병원성 및 일반조류인플루엔자 바이러스 항원 진단 스트립 및 그 제조방법 有权用于H5型高致病性流感病毒性流行性感冒病毒和使用快速免疫印迹的一般流行性流感病毒抗原的诊断条带及其制备方法
公开(公告)号：KR1020070072945A
公开(公告)日：2007-07-10
申请号：KR1020060000325
申请日：2006-01-03
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , 주식회사 바이오노트
IPC: G01N33/53
Abstract: A diagnostic strip for a H5 type highly pathogenic avian influenza and general avian influenza is provided to conveniently and rapidly diagnose whether a bird is infected by the H5 type highly pathogenic avian influenza and general avian influenza or not from stool in situ without special testing equipment. The diagnostic strip is characterized in that a monoclonal antibody common to general avian influenza virus in which hemaglutinin antigens are H1 to H15 and a monoclonal antibody specific to a highly pathogenic avian influenza in which a hemaglutinin antigen is H5 are used for diagnosing the highly pathogenic and the general avian influenza in which the hemaglutinin antigen is H5 from stool or tissue of chicken. The method for preparing the diagnostic strip comprises the steps of: (a) respectively absorbing the monoclonal antibody specific to the highly pathogenic avian influenza virus in which the hemaglutinin antigen is H5 and the monoclonal antibody common to general avian influenza virus into a membrane; (b) respectively attaching the antibody absorbed membrane obtained from the step(a) to gold particles so as to prepare an antibody-gold conjugate and then mixing it; and (c) precipitating the conjugate obtained from the step(b) in a basic material and then drying it.
Abstract translation: 提供一种H5型高致病性禽流感和一般禽流感的诊断条，以方便快捷地诊断鸟类是否由H5型高致病性禽流感和一般禽流感感染，或者不是在没有特殊检测设备的情况下从粪便中感染。 诊断条的特征在于，其中血凝素抗原为H1至H15的一般性禽流感病毒共有的单克隆抗体和其中血凝素抗原为H5的高致病性禽流感特异性的单克隆抗体用于诊断高致病性和 其中血凝素抗原为粪便或鸡组织中H5的一般性禽流感。 制备诊断条的方法包括以下步骤：（a）分别将其中血凝素抗原为H5的高致病性禽流感病毒和一般禽流感病毒共有的单克隆抗体特异性的单克隆抗体吸收到膜中; （b）将由步骤（a）获得的抗体吸收膜分别附着于金颗粒上，制备抗体 - 金缀合物，然后混合; 和（c）将由步骤（b）获得的缀合物沉淀在碱性材料中，然后干燥。
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公开(公告)号：KR1020070005867A
公开(公告)日：2007-01-10
申请号：KR1020050060920
申请日：2005-07-06
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: A virus strain for preparing a vaccine is provided to have excellent defending effect on low pathogenic avian influenza H9N2 virus strain. And a vaccine is provided to be able to effectively prevent diseases caused by the low pathogenic avian influenza H9N2 recording the high mortality of bird. The low pathogenic avian influenza H9N2 virus strain for preparing vaccine is characterized in that a motif of hemangioblast protein cleavage site amino acid sequence has TSGR and it shows the defending capacity against domestic low pathogenic avian influenza virus. The method for preparing a vaccine for the low pathogenic avian influenza H9N2 virus strain comprises the steps of: (a) inactivating the low pathogenic avian influenza H9N2 virus strain; and (b) mixing the inactivated virus with an adequate excipient.
Abstract translation: 提供用于制备疫苗的病毒株，以对低致病性禽流感H9N2病毒株具有优异的防御作用。 并提供疫苗能够有效预防由低致病性禽流感H9N2引起的疾病，记录鸟类的高死亡率。 用于制备疫苗的低致病性禽流感H9N2病毒株的特征在于成血管细胞蛋白切割位点氨基酸序列的基序具有TSGR，并且显示出针对国内低致病性禽流感病毒的防御能力。 用于制备低致病性禽流感H9N2病毒株的疫苗的方法包括以下步骤：（a）灭活低致病性禽流感H9N2病毒株; 和（b）将灭活的病毒与足够的赋形剂混合。
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Patent Agency Ranking
公开(公告)号：KR1020060132154A
公开(公告)日：2006-12-21
申请号：KR1020050052324
申请日：2005-06-17
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: A recombinant protein of avian influenza virus and a diagnostic composition of avian influenza containing the same protein are provided to mass produce the antigen for diagnosis of avian influenza cheaply, so that the disease caused by avian influenza is prevented. The method for producing the recombinant nucleus protein of avian influenza virus comprises the steps of: (1) amplifying the gene encoding nucleocapsid having the nucleotide sequence of SEQ ID NO:1 as a common protein of MS96 strain with PCR, and cloning the PCR amplified gene into the TA cloning vector; (2) re-isolating the NP gene inserted into the TA cloning vector with a restriction enzyme EcoR I, and inserting the isolated NP gene into the baculovirus transition vector pBacPAK8 to prepare the baculovirus transition vector pBacAINP; (3) co-transfecting the Sf21 cell with the baculovirus and the transition vector pBacAINP to a recombinant baculovirus(rAINP) expressing the avian influenza virus NP protein; and (4) verifying the expression of avian influenza virus NP(nucleus protein) in the Sf21 cell by using a monoclonal antibody specific to the avian influenza virus NP.
Abstract translation: 提供禽流感病毒的重组蛋白和含有相同蛋白质的禽流感诊断组合物，以便大量生产用于禽流感诊断的抗原，从而防止禽流感引起的疾病。 制备禽流感病毒重组核蛋白的方法包括以下步骤：（1）用PCR扩增编码具有SEQ ID NO：1的核苷酸序列的核衣壳的基因作为MS96菌株的常见蛋白质，并将PCR扩增 基因进入TA克隆载体; （2）用限制酶EcoR I重新分离插入TA克隆载体的NP基因，将分离的NP基因插入杆状病毒转移载体pBacPAK8中以制备杆状病毒转移载体pBacAINP; （3）将杆状病毒和过渡载体pBacAINP的Sf21细胞共转染至表达禽流感病毒NP蛋白的重组杆状病毒（rAINP）; 和（4）通过使用特异于禽流感病毒NP的单克隆抗体来验证Sf21细胞中禽流感病毒NP（核蛋白）的表达。
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公开(公告)号：KR1020100136191A
公开(公告)日：2010-12-28
申请号：KR1020090054422
申请日：2009-06-18
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: An antigen for hemoglutination for swine Japanese encephalitis virus is provided to reduce pollution caused by acetone extraction. CONSTITUTION: A method for preparing antigen for hemoglutination of high concentration for swine Japanese encephalitis virus comprises: a step of inoculating swine Japanese encephalitis virus to a tissue culture cells and planting; a step of freezing culture liquid; a step of thawing the culture liquid and repeating freezing and thawing once; a step of collecting cultured swine Japanese encephalitis virus; a step of inactivating swine Japanese encephalitis virus; a step of concentrating swine Japanese encephalitis virus; and a step of precipitating swine Japanese encephalitis virus by centrifugation.
Abstract translation: 目的：提供用于猪日本脑炎病毒血凝反应的抗原，以减少丙酮提取引起的污染。 构成：一种制备猪日本脑炎病毒高浓度血凝反应抗原的方法，包括：将日本脑炎病毒接种在组织培养细胞中并种植; 冷冻培养液的一个步骤; 解冻培养液并重复冻融一次; 收集日本猪日本脑炎病毒的一步; 灭活日本脑炎病毒的一步; 日本脑炎病毒浓缩一步; 并通过离心沉淀猪日本脑炎病毒的一个步骤。
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公开(公告)号：KR1020150134004A
公开(公告)日：2015-12-01
申请号：KR1020140060752
申请日：2014-05-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , 주식회사 인트론바이오테크놀로지
CPC classification number: C12Q1/686
Abstract: 본발명은개선된검출정확성을제공해줄 수있는핵산검사기반의조류인플루엔자바이러스검출및 H5형, H7형, 및 H9형동시감별검출키트에관한것이다. 본발명에따른조류인플루엔자바이러스검출및 H5형, H7형, 및 H9형동시감별검출키트는조류인플루엔자바이러스의목적유전자에서의짧은보존서열로인한문제점을극복할수 있는서열번호 1 내지서열번호 16으로특징지어지는특이프라이머들이적용되고, 또한기존조류인플루엔자바이러스검출용다중유전자증폭에서의민감도저하및 위음성발생문제를회피하기위하여 8개의튜브로구성된다중튜브방식이적용된것을특징으로한다. 본발명의조류인플루엔자바이러스검출및 H5형, H7형및 H9형을동시감별검출키트는조류인플루엔자바이러스검출과임상적으로주요한조류인플루엔자바이러스 H5형, 조류인플루엔자 H7형및 조류인플루엔자 H9형의동시감별검출에있어유용하게활용될수 있다.
Abstract translation: 本发明涉及用于禽流感病毒的试剂盒和基于具有改进的检测精度的核酸测试的H5-，H7-和H9-型同时差异检测。 根据本发明的试剂盒应用特征为SEQ ID NO：1至16的特异性引物，其可以克服由于禽流感病毒的靶基因中短的保守序列引起的问题; 并应用8管形成的多管法，以避免禽流感病毒检测现有多基因扩增中灵敏度降低和假阴性产生的问题。 根据本发明的试剂盒可以有用地用于禽流感病毒检测和H5型禽流感病毒，H7型禽流感病毒和H9型禽流感病毒的同时差异检测，这在临床上是重要的。
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9.发明公开조류인플루엔자 바이러스 검사용 프로브 조성물, 유전자 칩 그리고 이를 이용한 조류인플루엔자 바이러스 검사방법 有权用于分配AVIAN流感病毒的探针和DNA芯片以及使用其检测AVIAN流感病毒的方法
公开(公告)号：KR1020130116609A
公开(公告)日：2013-10-24
申请号：KR1020120039157
申请日：2012-04-16
Applicant: 바이오코아 주식회사 , 건국대학교 산학협력단 , 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: C12Q1/701 , C12Q1/04 , C12Q1/6837 , C12Q1/6844
Abstract: PURPOSE: A probe composition for detecting avian influenza virus is provided to simultaneously test the subtypes of HA gene and NA gene and pathogenicity and to quickly and accurately diagnose avian influenza virus. CONSTITUTION: A probe composition for determining serotype and pathogenicity of avian influenza virus contains: at least one probe selected among oligonucleotides with base sequences of sequence numbers 10-24 and complementary oligonucleotides thereof for determining the subtype of HA gene in avian influenza virus; at least one probe selected among oligonucleotides with base sequences of sequence numbers 27-46 and complementary oligonucleotides thereof for determining high pathogenicity of avian influenza virus; and at least one probe selected among oligonucleotides with base sequences of sequence numbers 47-63 and complementary oligonucleotides thereof for determining low pathogenicity of avian influenza virus.
Abstract translation: 目的：提供用于检测禽流感病毒的探针组合物，同时测试HA基因亚型和NA基因的致病性，并迅速准确地诊断禽流感病毒。 构成：用于测定禽流感病毒的血清型和致病性的探针组合物含有：选自具有序列号为10-24的碱基序列的寡核苷酸和其互补寡核苷酸的至少一种探针，用于确定禽流感病毒中HA基因的亚型; 选自具有序列号为27-46的碱基序列的寡核苷酸和其互补寡核苷酸的至少一个探针，用于确定禽流感病毒的高致病性; 和选自具有序列号47-63的碱基序列的寡核苷酸和其互补寡核苷酸中的至少一种探针，用于确定禽流感病毒的低致病性。
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公开(公告)号：KR100790801B1
公开(公告)日：2008-01-04
申请号：KR1020050060920
申请日：2005-07-06
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명은 HA 단백질 분절부위 아미노산 배열의 모티프가 TSGR을 가지며, 국내유행 저병원성 조류인플루엔자 바이러스에 방어능력을 나타내는 백신제조용 H9N2 혈청형의 저병원성 조류인플루엔자 바이러스주를 제공한다. 상기 바이러스주를 백신제조용 원료로 사용하는 경우 양계산업에 있어 산란율 저하 및 폐사 등 지속적인 문제를 일으키는 H9N2 혈청형 저병원성 조류인플루엔자 예방에 활용이 가능하다.
저병원성 조류인플루엔자, H9N2, 백신
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