公开(公告)号：KR100271850B1

公开(公告)日：2000-11-15

申请号：KR1019980004812

申请日：1998-02-17

Applicant: 대한민국(농촌진흥청장)

Inventor： 조성근 ,  노환국 ,  윤상기 ,  나기준 ,  이상진 ,  정일정

IPC: C12N1/20

Abstract: PURPOSE: A method for incubating lactic acid bacteria(LAB) isolated from the stomach of pigs is provided, thereby the activity decrease and denaturation of LAB can be inhibited during the incubation of lactic acid bacteria. CONSTITUTION: The lactic acid bacteria Lactobacillus casei (ATCC 334), which has high acid tolerance and antimicrobial activity, is isolated from the stomach of pigs. The Lactobacillus casei (ATCC 334) is incubated in an MRS medium further containing 0.003% of riboflavin and 0.005% of nicotinic acid as essential nutrients, 0.005% of hydrochloric acid and 0.001% of pyridoxal as growth stimulators in order to maintain its activity and not to occur denaturation of LAB during incubation, and eventually to reach to the intestine of animals when animals uptake LAB.

Abstract translation: 目的：提供从猪胃分离的乳酸菌（LAB）的培养方法，在乳酸菌培养过程中可以抑制LAB的活性降低和变性。 构成：具有高耐酸性和抗菌活性的乳酸菌干酪乳杆菌（ATCC 334）从猪的胃中分离出来。 将干酪乳杆菌（ATCC 334）在进一步含有0.003％核黄素和0.005％烟酸作为必需营养物，0.005％盐酸和0.001％吡哆醛作为生长刺激剂的MRS培养基中孵育，以保持其活性，而不是 在孵育期间发生LAB的变性，并且当动物摄取LAB时最终达到动物的肠。

公开(公告)号：KR100824348B1

公开(公告)日：2008-04-23

申请号：KR1020070006789

申请日：2007-01-23

Applicant: 대한민국(농촌진흥청장)

Inventor： 유용희 ,  정종원 ,  김태일 ,  최희철 ,  송준익 ,  양창범 ,  노환국 ,  감동환

IPC: A61G11/00

Abstract: An incubator for gnotobiotic mini pigs is provided to control the temperature and moisture, supply feed water in a sterilized state, and to grow mini pigs in an aseptic state. An incubator for aseptic mini pigs includes a temperature control device, a moisture control device, a ventilation device, an aseptic water manufacturing device, a feeding device(9), a sterilizing pass box(19), an entry port(8) and installation tools(3). The ventilation device maintains the atmosphere inside the incubator in an optical state by using an external air inflow filter(6) and a natural exhaust filter(12) respectively installed on the upper and lower parts of the incubator. The sterilizing pass box comprises a UV sterilizing system for supplying feed and equipment.

Abstract translation: 提供生物型微型猪的孵化器，以控制温度和湿度，供应无菌状态的饲料，并在无菌状态下种植微型猪。 无菌小型猪的培养箱包括温度控制装置，湿度控制装置，通风装置，无菌水制造装置，进料装置（9），消毒通过箱（19），入口（8）和安装 工具（3）。 通气装置通过使用分别安装在培养箱的上部和下部的外部空气流入过滤器（6）和自然排气过滤器（12）将培养箱内的气氛保持在光学状态。 消毒传送箱包括用于供应饲料和设备的UV灭菌系统。

公开(公告)号：KR1020030003462A

公开(公告)日：2003-01-10

申请号：KR1020010039282

申请日：2001-07-02

Applicant: 대한민국(농촌진흥청장)

Inventor： 김동운 ,  인영민 ,  정석근 ,  함준상 ,  채현석 ,  안종남 ,  이종문 ,  김용곤 ,  윤상기 ,  노환국

IPC: A61K38/00

Abstract: PURPOSE: Provided is a process of preparing casein protein hydrolysate which is not decomposed by angiotensin converting enzyme while having excellent inhibitory activity on angiotensin converting enzyme, by using an enzyme which acts in neutrality, thereby increasing productivity and reducing the cost of production. The product is convenient to eat and can be used as a protein supply source. CONSTITUTION: A protease is added to an aqueous casein protein solution in the amount of 0.1 to 3% and reacts at 30 to 50deg.C and a pH of 6 to 9 for 4 to 48hr. The protease is one or more selected from the group consisting of MP(Aspergillus melleus), Promod 192(derived from Aspergillus), Pescalase(Bacillus licheniformis), Protamex(derived from Bacillus) to produce hydrolyzed casein vegetable protein which lowers a blood pressure and has a molecular weight of 773 daltons.

Abstract translation: 目的：提供一种制备酪蛋白蛋白水解物的方法，其不受血管紧张素转化酶分解，同时对血管紧张素转换酶具有优异的抑制活性，通过使用以中性作用的酶，从而提高生产率并降低生产成本。 该产品方便食用，可作为蛋白质供应来源。 构成：将蛋白酶以0.1〜3％的量添加至含水酪蛋白溶液中，在30〜50℃，pH6〜9下反应4〜48小时。 蛋白酶是选自MP（曲霉曲霉），Promod 192（源自曲霉属），Pescalase（地衣芽孢杆菌），Protamex（衍生自芽孢杆菌属）的一种或多种，​​以产生降低血压的水解酪蛋白植物蛋白， 分子量为773道尔顿。

公开(公告)号：KR1019990070143A

公开(公告)日：1999-09-15

申请号：KR1019980004812

申请日：1998-02-17

Applicant: 대한민국(농촌진흥청장)

Inventor： 조성근 ,  노환국 ,  윤상기 ,  나기준 ,  이상진 ,  정일정

IPC: C12N1/20

Abstract: 본 발명은 돼지 위로 부터의 분리증식방법과 그에 의해 증식된 내산성 및 항균성이 우수한 유산균에 관한 것으로, 유산균을 배지에서 증식할 때 생체내와 다른 환경의 미적응 및 생체네 균합성 영양성 합성능력감소 및 배지내 구성된 제한된 영양소 조건하에서 오는 유산균의 활력감소 및 변성을 방지하도록 증식시의 필수 영양소로서 리보플라빈과 니코틴산 및 발육자극제로서 엽산과 피리독살을 첨가한 배지에서, 돼지 위 점막으로 부터 분리한 유산균을 증식시키는 것을 포함한다.

公开(公告)号：KR100470457B1

公开(公告)日：2005-02-05

申请号：KR1020010039283

申请日：2001-07-02

Applicant: 대한민국(농촌진흥청장)

Inventor： 김동운 ,  정석근 ,  인영민 ,  함준상 ,  채현석 ,  안종남 ,  이종문 ,  김용곤 ,  윤상기 ,  노환국

IPC: A61K38/00

Abstract: PURPOSE: A process of preparing egg white protein hydrolysate improved in flavoring by hydrolyzing egg white protein with protease is provided. Whereby, the product is not decomposed in the body by angiotensin converting enzyme while having excellent inhibitory activity on angiotensin converting enzyme, has excellent antihypertensive properties, is convenient to eat and can be used as a protein supply source. CONSTITUTION: A protease is added to an aqueous egg white protein solution in the amount of 0.1 to 5% and reacts at 30 to 50deg.C and a pH of 6 to 9 for 4 to 48hr. The protease is one or more selected from the group consisting of Protamex(derived from Bacillus), MP(Aspergillus melleus), LP(Aspergillus oryzae), Flavourzyme(Aspergillus oryzae), neutrase, alcalase, Bacillus licheniformis, Promod 192(derived from Aspergillus). For example, egg white is dissolved in water at 55 to 60deg.C, cooled and added with 1% Protamex at a pH of 7. Then the solution reacts at 47deg.C for 28hr and is maintained at 90deg.C for 15min to inactivate enzyme.

Abstract translation: 目的：提供一种通过用蛋白酶水解蛋白蛋白质来改进调味的蛋清蛋白水解物的制备方法。 由此，该产品在血管紧张素转换酶的作用下不在体内分解，对血管紧张素转化酶具有优异的抑制活性，抗高血压作用优良，食用方便，可作为蛋白质供应来源。 构成：将蛋白酶以0.1-5％的量添加到蛋清蛋白水溶液中，并在30-50℃和pH6-9下反应4-48小时。 蛋白酶选自由Protamex（来源于芽孢杆菌），MP（曲霉属），LP（米曲霉），Flavourzyme（米曲霉），中性蛋白酶，碱性蛋白酶，地衣芽孢杆菌，Promod192（来源于曲霉（Aspergillus） ）。 例如，将蛋清溶于55-60℃的水中，冷却并加入1％Protamex，pH值为7.然后该溶液在47℃反应28小时，并保持在90℃15分钟灭活 酶。

公开(公告)号：KR100470456B1

公开(公告)日：2005-02-05

申请号：KR1020010039282

申请日：2001-07-02

Applicant: 대한민국(농촌진흥청장)

Inventor： 김동운 ,  인영민 ,  정석근 ,  함준상 ,  채현석 ,  안종남 ,  이종문 ,  김용곤 ,  윤상기 ,  노환국

IPC: A61K38/00

Abstract: PURPOSE: Provided is a process of preparing casein protein hydrolysate which is not decomposed by angiotensin converting enzyme while having excellent inhibitory activity on angiotensin converting enzyme, by using an enzyme which acts in neutrality, thereby increasing productivity and reducing the cost of production. The product is convenient to eat and can be used as a protein supply source. CONSTITUTION: A protease is added to an aqueous casein protein solution in the amount of 0.1 to 3% and reacts at 30 to 50deg.C and a pH of 6 to 9 for 4 to 48hr. The protease is one or more selected from the group consisting of MP(Aspergillus melleus), Promod 192(derived from Aspergillus), Pescalase(Bacillus licheniformis), Protamex(derived from Bacillus) to produce hydrolyzed casein vegetable protein which lowers a blood pressure and has a molecular weight of 773 daltons.

Abstract translation: 目的：提供一种通过使用中性作用的酶制备不被血管紧张素转化酶分解，同时对血管紧张素转化酶具有优异抑制活性的酪蛋白蛋白水解产物的方法，由此提高生产率并降低生产成本。 该产品食用方便，可作为蛋白质供应来源。 组成：将蛋白酶以0.1-3％的量添加到含水酪蛋白溶液中，并在30-50℃和pH6-9下反应4-48小时。 该蛋白酶选自MP（曲霉曲霉），Promod 192（来源于曲霉），Pescalase（地衣芽孢杆菌），Protamex（来源于芽孢杆菌）以产生降低血压的水解酪蛋白植物蛋白和 分子量为773道尔顿。

公开(公告)号：KR100450588B1

公开(公告)日：2004-09-30

申请号：KR1020020016822

申请日：2002-03-27

Applicant: 대한민국(농촌진흥청장) ,  주식회사 다인엔지니어링

Inventor： 최희철 ,  권두중 ,  김형호 ,  곽정훈 ,  최동윤 ,  이덕수 ,  노환국

IPC: C02F1/78

Abstract: PURPOSE: A decolorization system of livestock wastewater effluent using ozone oxidation is provided to lower BOD, SS, T-N and T-P concentration in the effluent water. CONSTITUTION: The system comprises an ozone generator(10) for generating ozone, a set of ozone treatment tanks(3,4,5,6) connected to the ozone generator(10), submerged water pumps(3a,4a,5a,6a) installed in the corresponding ozone treatment tanks(3,4,5,6) for circulating water in the ozone treatment tanks to be in contact with ozone, an aeration tank(7) for exhausting remained ozone after ozone treatment of the livestock wastewater in the ozone treatment tanks, a sedimentation tank(8) for settling solid components in the ozone treated water, and an effluent tank arranged at an outlet side of the sedimentation tank(8) for discharging effluent water.

Abstract translation: 目的：利用臭氧氧化对畜禽废水进行脱色处理，以降低废水中的BOD，SS，T-N和T-P浓度。 构成：该系统包括用于产生臭氧的臭氧发生器（10），连接到臭氧发生器（10）的一组臭氧处理罐（3,4,5,6），浸没式水泵（3a，4a，5a，6a ）安装在相应的臭氧处理池（3,4,5,6）中，用于使臭氧处理池中的水循环以与臭氧接触;曝气池（7），用于在对臭氧处理后的剩余臭氧进行处理 臭氧处理槽，用于沉淀臭氧处理水中的固体成分的沉淀槽（8）和布置在沉淀槽（8）的出口侧用于排出流出水的流出槽。

公开(公告)号：KR1020030077865A

公开(公告)日：2003-10-04

申请号：KR1020020016822

申请日：2002-03-27

Applicant: 대한민국(농촌진흥청장) ,  주식회사 다인엔지니어링

Inventor： 최희철 ,  권두중 ,  김형호 ,  곽정훈 ,  최동윤 ,  이덕수 ,  노환국

IPC: C02F1/78

CPC classification number: C02F1/78 ,  C02F2103/20 ,  C02F2201/782 ,  C02F2201/784

Abstract: PURPOSE: A decolorization system of livestock wastewater effluent using ozone oxidation is provided to lower BOD, SS, T-N and T-P concentration in the effluent water. CONSTITUTION: The system comprises an ozone generator(10) for generating ozone, a set of ozone treatment tanks(3,4,5,6) connected to the ozone generator(10), submerged water pumps(3a,4a,5a,6a) installed in the corresponding ozone treatment tanks(3,4,5,6) for circulating water in the ozone treatment tanks to be in contact with ozone, an aeration tank(7) for exhausting remained ozone after ozone treatment of the livestock wastewater in the ozone treatment tanks, a sedimentation tank(8) for settling solid components in the ozone treated water, and an effluent tank arranged at an outlet side of the sedimentation tank(8) for discharging effluent water.

Abstract translation: 目的：提供使用臭氧氧化的畜禽粪便废水脱色系统，降低废水中的BOD，SS，T-N和T-P浓度。 构成：该系统包括用于产生臭氧的臭氧发生器（10），连接到臭氧发生器（10）的一组臭氧处理罐（3,4,5,6），浸没式水泵（3a，4a，5a，6a） ）安装在相应的臭氧处理罐（3,4,5,6）中，用于使臭氧处理罐中的水循环以与臭氧接触，用于在臭氧处理牲畜废水之后排出残留臭氧的曝气池（7） 臭氧处理罐，用于在臭氧处理水中沉降固体成分的沉淀池（8）和布置在沉淀池（8）的出口侧排出废水的流出罐。

公开(公告)号：KR1020030003463A

公开(公告)日：2003-01-10

申请号：KR1020010039283

申请日：2001-07-02

Applicant: 대한민국(농촌진흥청장)

Inventor： 김동운 ,  정석근 ,  인영민 ,  함준상 ,  채현석 ,  안종남 ,  이종문 ,  김용곤 ,  윤상기 ,  노환국

IPC: A61K38/00

Abstract: PURPOSE: A process of preparing egg white protein hydrolysate improved in flavoring by hydrolyzing egg white protein with protease is provided. Whereby, the product is not decomposed in the body by angiotensin converting enzyme while having excellent inhibitory activity on angiotensin converting enzyme, has excellent antihypertensive properties, is convenient to eat and can be used as a protein supply source. CONSTITUTION: A protease is added to an aqueous egg white protein solution in the amount of 0.1 to 5% and reacts at 30 to 50deg.C and a pH of 6 to 9 for 4 to 48hr. The protease is one or more selected from the group consisting of Protamex(derived from Bacillus), MP(Aspergillus melleus), LP(Aspergillus oryzae), Flavourzyme(Aspergillus oryzae), neutrase, alcalase, Bacillus licheniformis, Promod 192(derived from Aspergillus). For example, egg white is dissolved in water at 55 to 60deg.C, cooled and added with 1% Protamex at a pH of 7. Then the solution reacts at 47deg.C for 28hr and is maintained at 90deg.C for 15min to inactivate enzyme.

Abstract translation: 目的：提供通过用蛋白酶水解蛋白蛋白来改善调味的蛋白蛋白水解产物的方法。 由此，通过血管紧张素转换酶不会在体内分解产物，同时对血管紧张素转换酶具有优异的抑制活性，具有优异的抗高血压特性，方便食用，可用作蛋白质供应来源。 构成：以0.1〜5％的量向蛋白水溶液中加入蛋白酶，30〜50℃，pH6〜9，反应4〜48小时。 蛋白酶是选自由Protamex（源自芽孢杆菌属），MP（Aspergillus melleus），LP（米曲霉），Flavourzyme（米曲霉），中性酶，alcalase，地衣芽孢杆菌，Promod192（源自曲霉属）的组中的一种或多种 ）。 例如，将蛋清溶解于55〜60℃的水中，冷却，在pH7下加入1％的Protamex。然后溶液在47℃下反应28小时，保持在90℃，灭活15分钟 酶。