Abstract:
PURPOSE: Molecular markers and a detecting method using the same are provided to identify sequences of three subunits of bean beta-conglycinin by using a molecular marker, and to easily detect existence of three subunits of beta-conglycinin by directly extracting DNA from plant without conducting SDS-PAGE. CONSTITUTION: Primer pairs for selecting bean cultivar which detects a subunit of bean beta-conglycinin is selected from a group consisting of; a primer pair represented by sequence number 1 and 2; a primer pair represented by sequence number 3 and 4; a primer pair represented by sequence number 5 and 6; a primer pair represented by sequence number 7 and 8; a primer pair represented by sequence number 9 and 10; a primer pair represented by sequence number 11 and 12; and a primer pair represented by sequence number 13 and 14. A method of selecting bean cultivar which detects a subunit of bean beta-conglycinin comprises; (i) a step which separates DNA from a bean sample; (ii) a step which amplifies a target sequence by performing an amplification reaction with at least one primer pairs among the primer pairs and the separated DNA as a template; and (iii) a step which detects the amplified products.
Abstract:
본 발명은 광고 홍보용 벼논 식물 재배 방법에 관한 것으로서, 더욱 상세하게는 녹색벼와 유색벼를 동시에 이앙함으로써, 벼 생육기간인 약 4개월 동안 추가적인 비용없이 자연친화적으로 홍보할 수 있으며, 우리 농업의 중요성과 우리 쌀의 소중함을 보다 효과적으로 홍보할 수 있고, 동절기에 보리, 밀 등과 함께 자운영, 유채 등의 경관식물을 심어 연중 내내 벼논을 이용한 광고 홍보를 시행하고, 도로변이나 철로변의 심미적 가치를 높일 수 있는 광고 홍보용 벼논 식물 재배 방법에 관한 것이다. 이를 위해, 본 발명은 벼논에서 광고 홍보의 문구를 이루는 공간 및 배경 공간에 각각 다른 색을 띠는 종류의 식물을 심어 상기 광고 홍보의 문구가 시각적으로 선명하게 인식될 수 있도록 하는 광고 홍보용 벼논 식물 재배 방법을 제공한다. 광고 홍보, 벼논, 녹색벼, 유색벼, 경관식물, 보리, 벼
Abstract:
PURPOSE: A manufacturing method of a bean composition containing conjugated linoleic acid is provided to have the bean composition containing conjugated linoleic acid by solid fermenting a wet grinding material of bean after inoculating a mold or a mushroom mycelium. CONSTITUTION: A wet grinding material of bean which has 20-80 weight% of moisture content is manufactured by performing a water-logging and grinding the bean. A wet grinding material of bean is boiled to annihilate various germs. A boiled wet grinding material of bean is cooled and inoculated with a microorganism. A inoculated grinding material of bean is fermented at 10-50 °C for 7-30 days. The microorganism is made by cultivating a spawn after inoculating in a liquid culture medium in which rice and bean are mixed. The microorganism is Eumycetes or the mushroom mycelium. The wet grinding material of bean and microorganism is mixed as a ratio of (80-100) : (1-20) parts by weight. A linoleic acid or the cooking oil is additionally added in the grinding material of bean. The grinding material of bean is wet grinding material of bean which has 20-80 weight% of moisture content or a liquid grinding material in which the bean and moisture have a ratio of (6 - 20) parts by weight. [Reference numerals] (AA) Domestic bean raw material (variety: Deapung, Deauwl); (BB) Soaking in water and grinding; (CC) Manufacturing soybean milk; (DD) Cultivating on mold or mushroom mycelium; (EE,FF) Inoculation; (GG) Cultivating lactobacillus; (HH) Solid fermentation; (II) Lactobacillus fermentation; (JJ) Bean processed product with CLS; (KK) Bean yogurt with CLS
Abstract:
The present invention relates to a bean composition containing conjugated linoleic acid (CLA) and a method for producing the same. More specifically, the bean composition is produced in which linoleic acid is converted into CLA by adding microorganisms in a bean pulverized material so that the utilization of beans is maximized. Accordingly, the domestic production rate of beans is increased. CLA components are contained in biological activity functions of microorganisms which are beneficial for the human body so as to enhance efficiency in weight loss, cholesterol decrease, hyperlipidemia alleviation, and atherosclerosis alleviation. Accordingly, the bean composition can be utilized as functional food and a medical product.
Abstract:
A method of cultivating paddy field plants for advertizing in summer seasons is provided to make characters using various different colored paddy field plants, thereby using the paddy field plants for advertising. A method of cultivating paddy field plants for advertizing comprises the following steps of: planting colored rice(110) in a first space where an advertising message is shown; planting green rice in a background space(120); and planting the colored rice in the circumference of the first space. The colored rice represents purple rice whose leaf and stem are purple. The method of cultivating paddy field plants for advertizing in winter seasons comprises the following steps of: planting landscape plants in the first space; planting winter cereals plant in the background space; and re-planting the landscape plants in the circumference of the first space.
Abstract:
본 발명은 베타-콘글리시닌의 3-서브유닛을 판별하기 위한 분자표지 마커 및 이를 이용한 선별방법에 관한 것으로, 더욱 상세하게는 하나의 분자표지 마커를 이용하여 콩 단백질의 베타-콘글리시닌의 α,α',β 세가지 서브유닛에 대한 염기서열을 선별함으로써 콩 종자를 이용하여 단백질 전기영동(SDS-PAGE)을 할 필요 없이 식물체 잎에서 바로 DNA를 추출하여 베타-콘글리시닌의 3-서브유닛의 유무를 쉽게 알 수 있으며 분자표지를 이용하여 세대단축의 효과와 정확한 개체를 선발할 수 있어 많은 노동력과 비용절감의 효과가 있다. 뿐만 아니라 베타-콘글리시닌의 α와 α' 서브유닛이 제거된 유전자원을 이용하여 국내 우수품종과 교배한 개체는 수입콩과의 차별화를 이룰 수 있는 효과가 있는 베타-콘글리시닌의 3-서브유닛을 판별하기 위한 분자표지 마커 및 이를 이용한 선별방법에 관한 것이다.