전자기장을 이용하여 성체줄기세포를 슈반세포로 분화시키는 방법
    3.
    发明公开
    전자기장을 이용하여 성체줄기세포를 슈반세포로 분화시키는 방법 有权
    使用电磁场将成像干细胞分化成SCHWANN细胞的方法

    公开(公告)号:KR1020130099633A

    公开(公告)日:2013-09-06

    申请号:KR1020120021285

    申请日:2012-02-29

    Abstract: PURPOSE: A method for differentiating adult stem cells into schwann cells is provided to effectively differentiate adult stem cells into schwann cells without an expensive differentiation medium. CONSTITUTION: A method for differentiating adult stem cells into schwann cells using an electromagnetic field comprises the steps positioning the adult stem cells in the electromagnetic field at a frequency of 1-200 Hz; and culturing the adult stem cells. The intensity of the electromagnetic field is 0.5-2 mT. The culturing comprises the steps of: culturing the adult stem cells in an alpha-MEM medium containing beta-mercaptoethanol for 12-36 hours; culturing the adult stem cells in an alpha-MEM medium containing trans-retinoic acid for 60-82 hours; and culturing the adult stem cells in an alpha-MEM medium containing forskolin for 7-14 days.

    Abstract translation: 目的:提供一种将成体干细胞分化为雪旺氏细胞的方法,以有效地将成体干细胞分化成施旺细胞,而不需要昂贵的分化培养基。 构成:使用电磁场将成体干细胞分化成雪旺氏细胞的方法包括以1-200Hz的频率将成体干细胞定位在电磁场中的步骤; 并培养成体干细胞。 电磁场强度为0.5-2 mT。 培养包括以下步骤:在含有β-巯基乙醇的α-MEM培养基中培养12-36小时的成体干细胞; 在含有反式视黄酸的α-MEM培养基中培养成体干细胞60-82小时; 并在含有毛喉素的α-MEM培养基中培养成体干细胞7-14天。

    미세유체 기반의 조직 배양시 저산소 조건의 구현을 위한 세포 내 용존산소농도 제어장치 및 이를 이용한 세포 내 용존산소농도 제어방법
    4.
    发明授权

    公开(公告)号:KR101294521B1

    公开(公告)日:2013-08-07

    申请号:KR1020120015365

    申请日:2012-02-15

    CPC classification number: C12M41/48 C12M23/58 C12M41/34

    Abstract: PURPOSE: An apparatus for controlling the concentration of dissolved oxygen in cells and a method for controlling the concentration of dissolved oxygen in cells using the same are provided to simplify and miniaturize the apparatus because additional devices for injecting mixed gas are unnecessary. CONSTITUTION: An apparatus for controlling the concentration of dissolved oxygen in cells comprises: a first channel (110) which provides a space for cell culture; a second channel (120) which is placed at one side of the first channel; an intermediate membrane which is placed between the first and second channels and divides for enabling oxygen to pass through the first and second channels; and an oxygen absorbent supply unit which supplies an oxygen absorbent to the second channel for discharging intracellular dissolved oxygen of the first channel to the second channel through the intermediate membrane. A method for controlling the dissolved oxygen in cells using the apparatus comprises the steps of: culturing the cells in the first channel; and supplying the oxygen absorbent to the second channel and reducing oxygen partial pressure of the first channel.

    Abstract translation: 目的:提供一种用于控制细胞中溶解氧浓度的装置以及使用该装置控制细胞中溶解氧浓度的方法,以便简化和小型化装置,因为不需要额外的注入混合气体的装置。 构成:用于控制细胞中溶解氧浓度的装置包括:提供细胞培养空间的第一通道(110) 第二通道(120),其设置在所述第一通道的一侧; 中间膜,其被放置在第一通道和第二通道之间,并分开以使氧能够通过第一和第二通道; 以及氧吸收剂供给单元,其向第二通道供给氧吸收剂,用于通过中间膜将第一通道的细胞内溶解氧排出到第二通道。 使用该装置控制细胞中的溶解氧的方法包括以下步骤:在第一通道中培养细胞; 以及将氧吸收剂供应到第二通道并降低第一通道的氧分压。

    양극 산화와 연마를 이용한 표면 처리 방법
    6.
    发明公开
    양극 산화와 연마를 이용한 표면 처리 방법 无效
    使用阳极处理和抛光处理表面的方法

    公开(公告)号:KR1020110119100A

    公开(公告)日:2011-11-02

    申请号:KR1020100038605

    申请日:2010-04-26

    CPC classification number: C25D11/16 B24B37/04

    Abstract: PURPOSE: A surface treatment method using anodizing and polishing processes is provided to evenly form a nanoporous layer with reduced surface roughness by polishing a metal surface with diamonds and anodizing the metal surface. CONSTITUTION: A surface treatment method using anodizing and polishing is as follows. A metal surface is processed with a grinder and polished with diamonds. The polished surface is anodized. A nanoporous layer is formed on the metal surface without corrosion.

    Abstract translation: 目的:提供使用阳极氧化和抛光工艺的表面处理方法,通过用金刚石抛光金属表面并阳极氧化金属表面来均匀地形成具有降低的表面粗糙度的纳米多孔层。 构成:使用阳极氧化和抛光的表面处理方法如下。 用研磨机处理金属表面并用钻石抛光。 抛光表面经阳极氧化处理。 在金属表面上形成纳米多孔层而没有腐蚀。

    미세유체 소자를 이용한 세포손상 모델링 장치 및 이를 이용한 세포손상 모델링 방법
    8.
    发明授权
    미세유체 소자를 이용한 세포손상 모델링 장치 및 이를 이용한 세포손상 모델링 방법 有权
    使用微流体动力学和细胞损伤建模方法的细胞损伤建模装置

    公开(公告)号:KR101287690B1

    公开(公告)日:2013-07-24

    申请号:KR1020120015371

    申请日:2012-02-15

    CPC classification number: B01L3/5027 B01L2300/14 C12M23/16 C12N5/0619

    Abstract: PURPOSE: A cell damage modeling apparatus using a microfluidic element and a cell damage modeling method using the same are provided to simulate an environment which is similar to a real cell pressurizing condition through the configuration that is able to pressurize cells by supplying air to an air channel. CONSTITUTION: A cell damage modeling apparatus comprises: a cell culture channel (110) for culturing nerve cells; an air channel (120) formed at one side of the cell culture channel; an intermediate layer which is formed between the cell culture channel and the air channel and has an elastically transformable material; and an air supply unit which supplies air to the air channel to pressurize cells in the cell culture channel. The cell culture channel and the air channel form an interlayer in a main body (100) of a PDMS material.

    Abstract translation: 目的:提供使用微流体元件的细胞损伤造型装置和使用其的细胞损伤建模方法,以通过能够通过向空气供应空气来加压细胞的构造来模拟类似于真实细胞加压状态的环境 渠道。 构成:细胞损伤造型装置,包括:培养神经细胞的细胞培养通道(110) 形成在细胞培养通道一侧的空气通道(120) 中间层,其形成在细胞培养通道和空气通道之间,并具有可弹性变形的材料; 以及向空气通道供给空气以对细胞培养通道中的细胞加压的空气供给单元。 细胞培养通道和空气通道在PDMS材料的主体(100)中形成中间层。

    3차원 세포 배양 용구 및 이를 이용한 세포의 3차원 배양 방법
    9.
    发明公开
    3차원 세포 배양 용구 및 이를 이용한 세포의 3차원 배양 방법 有权
    3维细胞培养工具和细胞培养方法

    公开(公告)号:KR1020130033938A

    公开(公告)日:2013-04-04

    申请号:KR1020120050142

    申请日:2012-05-11

    CPC classification number: C12M23/12 C12M23/38 C12M35/06

    Abstract: PURPOSE: A three dimensional cell culture device and a three dimensional cell culture method using the same are provided to form a floating spheroid in a culture medium without a separate treatment such as inoculation of cells on a cover part. CONSTITUTION: A three dimensional cell culture device comprises a culture plate part, a cover part, and a protrusion part(50). The culture plate part has wells for culturing cells. The cover part covers the culture plate part. The protrusion part is extended toward the inside of the well from the surface which touches the culture plate part.

    Abstract translation: 目的:提供三维细胞培养装置和使用该三维细胞培养装置的三维细胞培养方法,以在培养基中形成浮球体,而无需单独处理,例如在盖部分上接种细胞。 构成:三维细胞培养装置包括培养板部分,盖部分和突出部分(50)。 培养板部分具有培养细胞的孔。 盖部覆盖培养板部。 突起部分从接触培养板部分的表面延伸到井的内部。

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