Abstract:
본 발명은 외부 인가 교류 자장에 의해 열을 발생시킬 수 있는 자성 입자(magnetic particle), PDMS(polydimethylsiloxane)를 포함하는 실리콘계 고분자 수지로 구성된 혼합조성물, 상기 혼합조성물로 만들어지고 외부 인가 교류 자장에 의해 열을 발생시킬 수 있는 발열구조체, 및 상기 발열구조체를 이용한 생물 화학 반응 방법에 관한 것이다. 본 발명의 혼합조성물은 고분자 수지를 포함하여 다양한 크기와 패턴을 가진 소자를 제작할 수 있고, 상기 혼합조성물로 만들어진 발열구조체는 자성 입자를 포함하여 외부 인가 교류자장에 의해 별도의 열원 없이 자체적으로 열을 발생시키는 특성을 가지고 있어, 유전자 증폭을 비롯한 다양한 생물 및 화학 실험에 응용 가능하다. 폴리디메틸실록산, 자성 입자, 히터, 교류 자장, 마이크로칩
Abstract:
PURPOSE: A mixture composition is provided to enable the control of temperature increase, to control energy consumption, and to obtain a heating element with accurate temperature control. CONSTITUTION: A mixture composition comprises magnetic particles and silicon-based polymer resin. The magnetic particles are included in the amount of 1~50 mass % based on the whole mixed compound. The magnetic particles are selected from the group consisting of Fe3O4, Fe2O3, aluminum, cobalt, nickel, manganese, tin, zinc, cadmium, magnesium, copper, barium, lithium and yttrium.
Abstract translation:目的:提供混合物组合物,以便能够控制温度升高,控制能量消耗,并获得具有精确温度控制的加热元件。 构成:混合物组合物包含磁性颗粒和硅基聚合物树脂。 基于整个混合化合物,磁性颗粒的含量为1〜50质量%。 磁性颗粒选自Fe 3 O 4,Fe 2 O 3,铝,钴,镍,锰,锡,锌,镉,镁,铜,钡,锂和钇。
Abstract:
PURPOSE: A method for differentiating adult stem cells into schwann cells is provided to effectively differentiate adult stem cells into schwann cells without an expensive differentiation medium. CONSTITUTION: A method for differentiating adult stem cells into schwann cells using an electromagnetic field comprises the steps positioning the adult stem cells in the electromagnetic field at a frequency of 1-200 Hz; and culturing the adult stem cells. The intensity of the electromagnetic field is 0.5-2 mT. The culturing comprises the steps of: culturing the adult stem cells in an alpha-MEM medium containing beta-mercaptoethanol for 12-36 hours; culturing the adult stem cells in an alpha-MEM medium containing trans-retinoic acid for 60-82 hours; and culturing the adult stem cells in an alpha-MEM medium containing forskolin for 7-14 days.
Abstract:
PURPOSE: An apparatus for controlling the concentration of dissolved oxygen in cells and a method for controlling the concentration of dissolved oxygen in cells using the same are provided to simplify and miniaturize the apparatus because additional devices for injecting mixed gas are unnecessary. CONSTITUTION: An apparatus for controlling the concentration of dissolved oxygen in cells comprises: a first channel (110) which provides a space for cell culture; a second channel (120) which is placed at one side of the first channel; an intermediate membrane which is placed between the first and second channels and divides for enabling oxygen to pass through the first and second channels; and an oxygen absorbent supply unit which supplies an oxygen absorbent to the second channel for discharging intracellular dissolved oxygen of the first channel to the second channel through the intermediate membrane. A method for controlling the dissolved oxygen in cells using the apparatus comprises the steps of: culturing the cells in the first channel; and supplying the oxygen absorbent to the second channel and reducing oxygen partial pressure of the first channel.
Abstract:
본 발명은 3차원 세포 배양 용구 및 이를 이용한 세포의 3차원 배양 방법 에 관한 것이다. 본 발명의 3차원 세포 배양 장치 및 이를 이용한 3차원 세포 배양 방법은 나노 입자가 포함된 배양액에서 세포를 기초 배양하여, 자성 나노 입자를 세포 내 또는 세포막에 도입시키고, 상기 자성 나노 입자가 도입된 세포에 자성을 인가하여 별도의 표면 처리 없이, 배양액 내의 부유 상태에서 스페로이드를 형성할 수 있는 효과를 나타낸다.
Abstract:
PURPOSE: A surface treatment method using anodizing and polishing processes is provided to evenly form a nanoporous layer with reduced surface roughness by polishing a metal surface with diamonds and anodizing the metal surface. CONSTITUTION: A surface treatment method using anodizing and polishing is as follows. A metal surface is processed with a grinder and polished with diamonds. The polished surface is anodized. A nanoporous layer is formed on the metal surface without corrosion.
Abstract:
본 발명은 전자기장을 이용하여 성체줄기세포를 슈반세포로 분화시키는 방법에 관한 것이다. 본 발명에 따른 성체줄기세포를 슈반세포로 분화시키는 방법은, 특정 주파수와 강도의 전자기장을 이용하여 성체줄기세포를 배양함으로써 고비용의 슈반세포 분화 배지를 사용하지 않고도 기존에 슈반세포로의 분화가 용이하지 않았던 성체줄기세포를 효과적으로 슈반세포로 분화시킬 수 있다.
Abstract:
PURPOSE: A cell damage modeling apparatus using a microfluidic element and a cell damage modeling method using the same are provided to simulate an environment which is similar to a real cell pressurizing condition through the configuration that is able to pressurize cells by supplying air to an air channel. CONSTITUTION: A cell damage modeling apparatus comprises: a cell culture channel (110) for culturing nerve cells; an air channel (120) formed at one side of the cell culture channel; an intermediate layer which is formed between the cell culture channel and the air channel and has an elastically transformable material; and an air supply unit which supplies air to the air channel to pressurize cells in the cell culture channel. The cell culture channel and the air channel form an interlayer in a main body (100) of a PDMS material.
Abstract:
PURPOSE: A three dimensional cell culture device and a three dimensional cell culture method using the same are provided to form a floating spheroid in a culture medium without a separate treatment such as inoculation of cells on a cover part. CONSTITUTION: A three dimensional cell culture device comprises a culture plate part, a cover part, and a protrusion part(50). The culture plate part has wells for culturing cells. The cover part covers the culture plate part. The protrusion part is extended toward the inside of the well from the surface which touches the culture plate part.