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    피타제 생산 효모 균주를 함유하는 사료용 조성물
    2.
    发明授权
    피타제 생산 효모 균주를 함유하는 사료용 조성물 有权
    피타제생산효모균주를함유하는사료용조성물

    公开(公告)号：KR100375673B1

    公开(公告)日：2003-03-15

    申请号：KR1020000004429

    申请日：2000-01-29

    Applicant: 대한민국(농촌진흥청장)

    Inventor： 이현정 ,  이상철 ,  김진욱 ,  신기준 ,  최윤재 ,  강승하 ,  복진덕

    IPC: C12N15/63

    Abstract: PURPOSE: Provided is a recombinant vector pGAP-phyA including phytase gene coding myo-inositol hexaphosphate phosphohydrolase derived from gram-positive bacteria, Enterobactor sp.4. And transformant Pichia pastoris pGAP-phyA is also provided, which can secret myo-inositol hexaphosphate phosphohydrolase continuously, and is useful as a feed additive. CONSTITUTION: A recombinant vector pGAP-phyA including phytase gene coding myo-inositol hexaphosphate phosphohydrolase is prepared by the steps of: (a) separating myo-inositol hexaphosphate phosphohydrolase chromosome derived from Enterobactor sp.4; (b) cloning and sequencing the base sequence of the gene coding myo-inositol hexaphosphate phosphohydrolase originated from gram-positive bacteria, Enterobactor sp.4; (c) constructing an expression vector including a gene fragment for coding myo-inositol hexaphosphate phosphohydrolase; (d) transforming Pichia pastoris GS 115 yeast strain with the vector; and (e) selecting a transformant.

    Abstract translation: 目的：提供一种重组载体pGAP-phyA，其包括编码肌酸六磷酸磷酸水解酶的肌醇六磷酸酶基因，所述肌醇六磷酸磷酸水解酶来源于革兰氏阳性细菌Enterobactor sp.4。 还提供了转化体巴斯德毕赤酵母pGAP-phyA，其可以连续分泌肌醇六磷酸磷酸水解酶，并且可用作饲料添加剂。 构成：通过以下步骤制备包含编码肌醇六磷酸磷酸水解酶的植酸酶基因的重组载体pGAP-phyA：（a）分离源自Enterobactor sp.4的肌醇六磷酸磷酸水解酶染色体; （b）克隆和测序编码源自革兰氏阳性细菌肌醇六磷酸磷酸水解酶的基因的碱基序列，Enterobactor sp.4; （c）构建包含编码肌肉六磷酸磷酸水解酶的基因片段的表达载体; （d）用载体转化巴斯德毕赤酵母GS115酵母菌株; 和（e）选择转化体。

    피타제 생산 효모 균주를 함유하는 사료용 조성물
    3.
    发明公开
    피타제 생산 효모 균주를 함유하는 사료용 조성물 有权
    含有来自ENTEROPACTER SP.4的含有PHYTASE基因的重组载体和包含它的变体

    公开(公告)号：KR1020010076962A

    公开(公告)日：2001-08-17

    申请号：KR1020000004429

    申请日：2000-01-29

    Applicant: 대한민국(농촌진흥청장)

    Inventor： 이현정 ,  이상철 ,  김진욱 ,  신기준 ,  최윤재 ,  강승하 ,  복진덕

    IPC: C12N15/63

    CPC classification number: C12N15/63 ,  A23K10/16 ,  A61K35/741

    Abstract: PURPOSE: Provided is a recombinant vector pGAP-phyA including phytase gene coding myo-inositol hexaphosphate phosphohydrolase derived from gram-positive bacteria, Enterobactor sp.4. And transformant Pichia pastoris pGAP-phyA is also provided, which can secret myo-inositol hexaphosphate phosphohydrolase continuously, and is useful as a feed additive. CONSTITUTION: A recombinant vector pGAP-phyA including phytase gene coding myo-inositol hexaphosphate phosphohydrolase is prepared by the steps of: (a) separating myo-inositol hexaphosphate phosphohydrolase chromosome derived from Enterobactor sp.4; (b) cloning and sequencing the base sequence of the gene coding myo-inositol hexaphosphate phosphohydrolase originated from gram-positive bacteria, Enterobactor sp.4; (c) constructing an expression vector including a gene fragment for coding myo-inositol hexaphosphate phosphohydrolase; (d) transforming Pichia pastoris GS 115 yeast strain with the vector; and (e) selecting a transformant.

    Abstract translation: 目的：提供一种重组载体pGAP-phyA，其中包括植酸酶基因，其编码来自革兰氏阳性菌的Entero反应器sp.4的肌醇六磷酸磷酸水解酶。 还提供了转化体巴斯德毕赤酵母pGAP-phyA，其可以连续分泌肌醇六磷酸磷酸水解酶，并且可用作饲料添加剂。 构成：通过以下步骤制备包含编码肌醇六磷酸磷酸水解酶的肌醇六磷酸酶基因的重组载体pGAP-phyA：（a）分离源自Enterobactor sp.4的肌醇六磷酸磷酸水解酶染色体; （b）克隆和测序来源于革兰氏阳性菌的Enterobactor sp.4的编码肌醇六磷酸磷酸水解酶的基因的碱基序列; （c）构建包含编码肌醇六磷酸磷酸水解酶的基因片段的表达载体; （d）用载体转化巴斯德毕赤酵母GS 115酵母菌株; 和（e）选择转化体。

    오메가 지방산의 균형과 베타인이 강화된 기능성 돈육 및 가공육 생산과 그 생산방법
    4.
    发明公开
    오메가 지방산의 균형과 베타인이 강화된 기능성 돈육 및 가공육 생산과 그 생산방법 审中-实审
    欧米茄脂肪酸的平衡以及功能性猪肉和加工肉类的生产，增加甜菜碱和生产方法

    公开(公告)号：KR1020170077936A

    公开(公告)日：2017-07-07

    申请号：KR1020150187693

    申请日：2015-12-28

    Applicant: 서울대학교산학협력단

    Inventor： 최윤재 ,  조종수 ,  강상기 ,  복진덕 ,  이윤석

    IPC: A23K1/16 ,  A23K1/10 ,  A23L13/00

    Abstract: 본발명은오메가지방산의균형을맞추고베타인을강화시킨돈육및 가공육과그 생산방법에관한것이다. 상기돈육과가공육은오메가지방산의균형을맞추고베타인을강화시킴으로써비육돈의여름철고온스트레스저감효과및 생리활성효과가있으며균형을맞춘오메가지방산과베타인을돈육내 축적시킴으로써기능성돈육을소비자에게전달할수 있다. 또, 선호부위인삼겹살이외에비선호부위인후지를이용한가공육제품을제공함으로써비선호부위의소비진작에도도움을주는뛰어난효과가있다.

    Abstract translation: 本发明涉及与ω-脂肪酸平衡并用甜菜碱强化的猪肉和加工肉及其制造方法。 猪肉和加工的肉可以是由夏天热应力降低效果，并且整理器的生理活性的效果，并且，ω脂肪酸和甜菜碱均衡猪肉通过增强甜菜碱平衡的欧米加脂肪酸内积累通过猪肉功能给消费者。 此外，有极好的效果，有助于通过使用富士另外的非优选的站点是优选的位点提供肚加工肉制品刺激的非优选区域的消耗。

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