公开(公告)号：KR1020040074108A

公开(公告)日：2004-08-21

申请号：KR1020047010333

申请日：2001-12-29

Applicant: 재단법인서울대학교산학협력재단

Inventor： 황우석 ,  이병천 ,  강성근 ,  한재용 ,  임정묵 ,  이창규 ,  이은송 ,  정의배 ,  조종기 ,  김대영 ,  현상환 ,  이갑상 ,  김혜수 ,  이소현 ,  이성철 ,  염수청

IPC: A01K67/02 ,  C12N5/07 ,  C12N15/63

Abstract: PURPOSE: A GFP(Green Fluorescent Protein)-transfected clone pig, a GT(alpha 1, 3 galactrosyltransferase) knock-out clone pig and a production method thereof are provided to produce a clone pig with a determined genetic character using transfection and targeting technologies. CONSTITUTION: The production method of the GFP-transfected clone pig comprises the steps of: preparing a cell line taken and cultured from a pig as a nucleus donating cell; introducing the GFP clone to the nucleus donating cell and expressing by forming a lipid(or a positive charge polymer)-DNA structure complex, adding the complex to the nucleus donating cell culture solution and culturing; producing a transfected embryo by transplanting the cell to an enucleated pig embryo and activating; and producing a piglet by transplanting the embryo to a surrogate mother.

Abstract translation: 目的：提供GFP（绿色荧光蛋白）转染的克隆猪，GT（α1,3半乳糖基转移酶）敲除克隆猪及其制备方法，以使用转染和靶向技术产生具有确定遗传特征的克隆猪 。 构成：GFP转染的克隆猪的制备方法包括以下步骤：制备从猪获得并培养为细胞核的细胞系; 将GFP克隆引入提供细胞核并通过形成脂质（或正电荷聚合物）-DNA结构复合物进行表达，将复合物加入给予细胞培养液的细胞核并培养; 通过将细胞移植到去核的猪胚胎并激活来产生转染的胚胎; 并通过将胚胎移植到替代母亲来生产仔猪。