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1.发明授权씀바귀뿌리 열수 추출물로부터 저분자량엔지오텐신전환효소 저해제를 제조하는 방법 및 상기 방법에 의해 제조된 저분자량엔지오텐신전환효소 저해제를 함유하는 식품 有权씀바귀뿌리열수추출물로부터저분자량엔지오텐신전환효소저해제를제조하는방법및상기방법에의해제조된저분자량엔지오텐신전환효소저해제를함유하는식품
公开(公告)号：KR100447534B1
公开(公告)日：2004-09-08
申请号：KR1020010023416
申请日：2001-04-30
Applicant: 충남대학교산학협력단
Abstract: PURPOSE: A manufacturing process for a low molecular weight of angiotensin converting enzyme(ACE) inhibitor from hot water extract of lettuce root is provided, which is an efficient and economical process. CONSTITUTION: The manufacturing process for a low molecular weight of ACE inhibitor from lettuce root comprises the steps of: removing an impurities by washing lettuce, separating its root, adding distilled water to the root, and extracting by making reflux for 2hrs at 100deg.C, 60deg.C; filtering, centrifuging for 30min. at 4deg.C, 8000rpm, and collecting the upper solution; ultra-filtering the upper solution using ultra-filter membrane(limit of M.W. is 1000) to prepare the extract; filtering through membrane filter paper, loading on high performance liquid chromatography, and separating a fraction, F4 with highest activity; and then gathering the F4, freeze-drying, and purifying using reversed phase HPLC.
Abstract translation: 目的：提供一种低分子量的生菜根热水提取物中的血管紧张素转化酶（ACE）抑制剂的制造工艺，这是一种高效且经济的工艺。 组成：低分子量生菜根ACE抑制剂的制造工艺包括以下步骤：通过洗涤生菜，分离其根，将蒸馏水加入根中，并通过在100℃回流2小时进行提取来除去杂质。 ，60deg.C; 过滤，离心30分钟。 在4℃，8000rpm下，收集上层溶液; 使用超滤膜（M.W.的极限为1000）超滤上层溶液以制备提取物; 通过膜滤纸过滤，上样于高效液相色谱仪，并分离出具有最高活性的馏分F4; 然后收集F4，冷冻干燥，并使用反相HPLC纯化。
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2.发明公开씀바귀뿌리 열수 추출물로부터 저분자량엔지오텐신전환효소 저해제를 제조하는 방법 및 상기 방법에 의해 제조된 저분자량엔지오텐신전환효소 저해제를 함유하는 식품 有权高分子量转氨酶抑制剂低分子量制备工艺从热水提取物
公开(公告)号：KR1020010068159A
公开(公告)日：2001-07-13
申请号：KR1020010023416
申请日：2001-04-30
Applicant: 충남대학교산학협력단
Abstract: PURPOSE: A manufacturing process for a low molecular weight of angiotensin converting enzyme(ACE) inhibitor from hot water extract of lettuce root is provided, which is an efficient and economical process. CONSTITUTION: The manufacturing process for a low molecular weight of ACE inhibitor from lettuce root comprises the steps of: removing an impurities by washing lettuce, separating its root, adding distilled water to the root, and extracting by making reflux for 2hrs at 100deg.C, 60deg.C; filtering, centrifuging for 30min. at 4deg.C, 8000rpm, and collecting the upper solution; ultra-filtering the upper solution using ultra-filter membrane(limit of M.W. is 1000) to prepare the extract; filtering through membrane filter paper, loading on high performance liquid chromatography, and separating a fraction, F4 with highest activity; and then gathering the F4, freeze-drying, and purifying using reversed phase HPLC.
Abstract translation: 目的：提供莴苣根热水提取物中低分子量血管紧张素转换酶（ACE）抑制剂的制备方法，这是一个有效和经济的过程。 构成：莴苣根的低分子量ACE抑制剂的制造过程包括以下步骤：通过洗莴苣，分离根，向根中加入蒸馏水，并在100℃回流2小时提取，除去杂质。 ，60deg.C 过滤，离心30min。 在4℃，8000rpm下收集上层溶液; 使用超滤膜（M.W.的限制为1000）对上部溶液进行超滤，以制备提取物; 通过膜滤纸过滤，装载高效液相色谱法，分离出一个具有最高活性的级分F4; 然后使用反相HPLC收集F4，冷冻干燥和纯化。
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公开(公告)号：KR101721063B1
公开(公告)日：2017-03-29
申请号：KR1020160163392
申请日：2016-12-02
Applicant: 충남대학교산학협력단
Abstract: 본발명에의한개인정보검색방법은, 이미지파일에서텍스트정보를추출하는제1 단계; 텍스트정보가개인정보일확률을계산하는제2 단계; 제2 단계의확률값이 1차기준값미만이면개인정보가아닌것으로판단하는제3 단계; 제2 단계의확률값이 1차기준값이상이면반복학습을통해텍스트인식정확도를향상시키는제4 단계; 제4 단계에서인식된텍스트로개인정보일확률을계산하는제5 단계; 제5 단계의확률값이 2차기준값이상이면개인정보인것으로판단하는제6 단계;를포함하는것을특징으로한다.
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