公开(公告)号：KR100458965B1

公开(公告)日：2004-12-03

申请号：KR1020020011577

申请日：2002-03-05

Applicant: 충남대학교산학협력단

Inventor： 송경빈 ,  원미선 ,  이승환 ,  이승현 ,  선남규

IPC: A23L1/312

Abstract: PURPOSE: Provided is a manufacture of bovine and porcine plasma protein powders by treating low dose of gamma-ray, thereby preventing contamination by microorganisms, and removing biological risk factors while maintaining physicochemical properties of the plasma protein. CONSTITUTION: A manufacture of bovine and porcine plasma protein powders comprises the steps of: separating plasma from bovine and porcine using a centrifuger; precipitating the plasma using 2-10% trichloroacetic acid, 0.6-3N of hydrochloric acid, 50-70% of ammonium sulfate and the like; drying the precipitated plasma to give powder thereof; and packing the powder with polyethylene packing material and treating gamma-ray.

Abstract translation: 目的：提供一种通过处理低剂量的γ射线来制造牛和猪血浆蛋白质粉末，从而防止微生物污染，并且在保持血浆蛋白质的物理化学性质的同时去除生物危险因素。 组成：牛和猪血浆蛋白粉的生产包括以下步骤：用离心机从牛和猪中分离血浆; 使用2-10％三氯乙酸，0.6-3N盐酸，50-70％硫酸铵等沉淀血浆; 干燥沉淀的等离子体以得到其粉末; 并用聚乙烯包装材料包装粉末并处理γ射线。

公开(公告)号：KR1020030072044A

公开(公告)日：2003-09-13

申请号：KR1020020011577

申请日：2002-03-05

Applicant: 충남대학교산학협력단

Inventor： 송경빈 ,  원미선 ,  이승환 ,  이승현 ,  선남규

IPC: A23L1/312

Abstract: PURPOSE: Provided is a manufacture of bovine and porcine plasma protein powders by treating low dose of gamma-ray, thereby preventing contamination by microorganisms, and removing biological risk factors while maintaining physicochemical properties of the plasma protein. CONSTITUTION: A manufacture of bovine and porcine plasma protein powders comprises the steps of: separating plasma from bovine and porcine using a centrifuger; precipitating the plasma using 2-10% trichloroacetic acid, 0.6-3N of hydrochloric acid, 50-70% of ammonium sulfate and the like; drying the precipitated plasma to give powder thereof; and packing the powder with polyethylene packing material and treating gamma-ray.

Abstract translation: 目的：提供通过治疗低剂量γ射线的牛和猪血浆蛋白粉的制造，从而防止微生物的污染，并且在维持血浆蛋白质的物理化学性质的同时去除生物危险因素。 构成：牛和猪血浆蛋白粉的制造包括以下步骤：使用离心机从牛和猪分离血浆; 使用2-10％三氯乙酸，0.6-3N盐酸，50-70％硫酸铵等沉淀血浆; 干燥沉淀的等离子体，得到粉末; 并用聚乙烯包装材料包装粉末并处理γ射线。

公开(公告)号：KR1020010068159A

公开(公告)日：2001-07-13

申请号：KR1020010023416

申请日：2001-04-30

Applicant: 충남대학교산학협력단

Inventor： 송경빈 ,  원미선 ,  정혜영 ,  김숙현 ,  김지연 ,  선남규 ,  이진희 ,  이승환

IPC: A61K36/28 ,  A61P9/12

Abstract: PURPOSE: A manufacturing process for a low molecular weight of angiotensin converting enzyme(ACE) inhibitor from hot water extract of lettuce root is provided, which is an efficient and economical process. CONSTITUTION: The manufacturing process for a low molecular weight of ACE inhibitor from lettuce root comprises the steps of: removing an impurities by washing lettuce, separating its root, adding distilled water to the root, and extracting by making reflux for 2hrs at 100deg.C, 60deg.C; filtering, centrifuging for 30min. at 4deg.C, 8000rpm, and collecting the upper solution; ultra-filtering the upper solution using ultra-filter membrane(limit of M.W. is 1000) to prepare the extract; filtering through membrane filter paper, loading on high performance liquid chromatography, and separating a fraction, F4 with highest activity; and then gathering the F4, freeze-drying, and purifying using reversed phase HPLC.

Abstract translation: 目的：提供莴苣根热水提取物中低分子量血管紧张素转换酶（ACE）抑制剂的制备方法，这是一个有效和经济的过程。 构成：莴苣根的低分子量ACE抑制剂的制造过程包括以下步骤：通过洗莴苣，分离根，向根中加入蒸馏水，并在100℃回流2小时提取，除去杂质。 ，60deg.C 过滤，离心30min。 在4℃，8000rpm下收集上层溶液; 使用超滤膜（M.W.的限制为1000）对上部溶液进行超滤，以制备提取物; 通过膜滤纸过滤，装载高效液相色谱法，分离出一个具有最高活性的级分F4; 然后使用反相HPLC收集F4，冷冻干燥和纯化。

公开(公告)号：KR100447534B1

公开(公告)日：2004-09-08

申请号：KR1020010023416

申请日：2001-04-30

Applicant: 충남대학교산학협력단

Inventor： 송경빈 ,  원미선 ,  정혜영 ,  김숙현 ,  김지연 ,  선남규 ,  이진희 ,  이승환

IPC: A61K36/28 ,  A61P9/12

Abstract: PURPOSE: A manufacturing process for a low molecular weight of angiotensin converting enzyme(ACE) inhibitor from hot water extract of lettuce root is provided, which is an efficient and economical process. CONSTITUTION: The manufacturing process for a low molecular weight of ACE inhibitor from lettuce root comprises the steps of: removing an impurities by washing lettuce, separating its root, adding distilled water to the root, and extracting by making reflux for 2hrs at 100deg.C, 60deg.C; filtering, centrifuging for 30min. at 4deg.C, 8000rpm, and collecting the upper solution; ultra-filtering the upper solution using ultra-filter membrane(limit of M.W. is 1000) to prepare the extract; filtering through membrane filter paper, loading on high performance liquid chromatography, and separating a fraction, F4 with highest activity; and then gathering the F4, freeze-drying, and purifying using reversed phase HPLC.

Abstract translation: 目的：提供一种低分子量的生菜根热水提取物中的血管紧张素转化酶（ACE）抑制剂的制造工艺，这是一种高效且经济的工艺。 组成：低分子量生菜根ACE抑制剂的制造工艺包括以下步骤：通过洗涤生菜，分离其根，将蒸馏水加入根中，并通过在100℃回流2小时进行提取来除去杂质。 ，60deg.C; 过滤，离心30分钟。 在4℃，8000rpm下，收集上层溶液; 使用超滤膜（M.W.的极限为1000）超滤上层溶液以制备提取物; 通过膜滤纸过滤，上样于高效液相色谱仪，并分离出具有最高活性的馏分F4; 然后收集F4，冷冻干燥，并使用反相HPLC纯化。