Abstract:
본 발명은 식중독균 검출 방법에 관한 것으로서, 보다 상세하게는 음식물 등에 오염된 식중독균의 함량을 정량적으로 신속하게 분리할 수 있는 방법에 관한 것이다. 본 발명에 따른 방법은 세균을 측정하는 시료에 상기 세균에 결합 가능한 자성 나노입자를 투입하여 세균에 상기 자성 나노입자를 결합시키는 단계; 자성 나노입자를 분리하는 단계; 자력을 이용하여 분리된 자성 나노입자를 고점성 액체에 투과시켜 세균이 결합된 자성 나노입자와 세균이 결합되지 않는 자성 나노입자를 분리하는 단계; 및 세균이 결합된 자성 나노입자를 정량하는 단계를 포함하는 것을 특징으로 한다.
Abstract:
The present invention relates to a method for detecting food borne pathogens and, more specifically, to a method for rapidly and quantitatively isolating contents of food borne pathogens contaminated in foods. In order to solve the assignment, the method according to the present invention comprises the following steps: adding magnetic nanoparticles capable of combining with bacteria to samples which measure the bacteria, and combining the magnetic nanoparticles with the bacteria; isolating the magnetic nanoparticles; separating the magnetic nanoparticles combined with the bacteria from magnetic nanoparticles not combined with the bacteria by transmitting the magnetic nanoparticles isolated using magnetism through a high viscous liquid; and quantifying the magnetic nanoparticles combined with the bacteria. As in the present invention, the method for isolating the magnetic nanoparticles using magnetism from high viscous liquid clearly isolates the magnetic nanoparticles with which bacteria are not combined from the magnetic nanoparticles with which bacteria are combined while showing band shapes in the high viscous liquid. If centrifugal separator is used, separation capability between nanoparticles is decreased. In addition, the method according to the present invention does not require a separate device such as the centrifugal separator, except magnetic nanoparticles and magnets.