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公开(公告)号：WO2014081265A1
公开(公告)日：2014-05-30
申请号：PCT/KR2013/010765
申请日：2013-11-26
Applicant: 포항공과대학교 산학협력단 , 대한민국(식품의약품안전처장)
IPC: C12Q1/04 , C12Q1/24 , G01N33/569
CPC classification number: G01N33/54333 , C12Q1/04 , C12Q1/24 , G01N33/54326 , G01N33/54346 , G01N33/56911 , G01N33/56916 , G01N33/587 , G01N2333/255
Abstract: 본 발명은 식중독균 검출 방법에 관한 것으로서, 보다 상세하게는 음식물 등에 오염된 식중독균의 함량을 정량적으로 신속하게 분리할 수 있는 방법에 관한 것이다. 본 발명에 따른 방법은 세균을 측정하는 시료에 상기 세균에 결합 가능한 자성 나노입자를 투입하여 세균에 상기 자성 나노입자를 결합시키는 단계; 자성 나노입자를 분리하는 단계; 자력을 이용하여 분리된 자성 나노입자를 고점성 액체에 투과시켜 세균이 결합된 자성 나노입자와 세균이 결합되지 않는 자성 나노입자를 분리하는 단계; 및 세균이 결합된 자성 나노입자를 정량하는 단계를 포함하는 것을 특징으로 한다.
Abstract translation: 本发明涉及一种检测食物中毒细菌的方法，更具体地，涉及一种快速，定量分离污染食物等的食物中毒细菌成分的方法。 根据本发明的方法的特征在于包括以下步骤：将可与细菌结合的磁性纳米颗粒引入用于测量细菌的样品中，以将磁性纳米颗粒结合到细菌上; 分离磁性纳米粒子; 将通过使用磁性分离的纳米颗粒通过具有高粘度的溶液通过，以将与细菌结合的磁性纳米颗粒结合的磁性纳米颗粒分离; 并定量细菌所结合的磁性纳米颗粒。
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公开(公告)号：KR1020140067386A
公开(公告)日：2014-06-05
申请号：KR1020120134544
申请日：2012-11-26
Applicant: 포항공과대학교 산학협력단 , 대한민국 (식품의약품안전처장)
IPC: C12Q1/04 , C12Q1/24 , G01N33/569
CPC classification number: G01N33/54333 , C12Q1/04 , C12Q1/24 , G01N33/54326 , G01N33/54346 , G01N33/56911 , G01N33/56916 , G01N33/587 , G01N2333/255
Abstract: The present invention relates to a method for detecting food borne pathogens and, more specifically, to a method for rapidly and quantitatively isolating contents of food borne pathogens contaminated in foods. In order to solve the assignment, the method according to the present invention comprises the following steps: adding magnetic nanoparticles capable of combining with bacteria to samples which measure the bacteria, and combining the magnetic nanoparticles with the bacteria; isolating the magnetic nanoparticles; separating the magnetic nanoparticles combined with the bacteria from magnetic nanoparticles not combined with the bacteria by transmitting the magnetic nanoparticles isolated using magnetism through a high viscous liquid; and quantifying the magnetic nanoparticles combined with the bacteria. As in the present invention, the method for isolating the magnetic nanoparticles using magnetism from high viscous liquid clearly isolates the magnetic nanoparticles with which bacteria are not combined from the magnetic nanoparticles with which bacteria are combined while showing band shapes in the high viscous liquid. If centrifugal separator is used, separation capability between nanoparticles is decreased. In addition, the method according to the present invention does not require a separate device such as the centrifugal separator, except magnetic nanoparticles and magnets.
Abstract translation: 本发明涉及一种用于检测食源性病原体的方法，更具体地，涉及一种用于快速和定量地分离食品中食源性病原体的内容物的方法。 为了解决这一问题，本发明的方法包括以下步骤：将能够与细菌结合的磁性纳米颗粒加入到测量细菌的样品中，并将磁性纳米颗粒与细菌结合; 分离磁性纳米粒子; 通过传输使用磁性分离的磁性纳米颗粒通过高粘度液体将与细菌结合的磁性纳米颗粒与未与细菌组合的磁性纳米颗粒分离; 并量化与细菌结合的磁性纳米颗粒。 与本发明一样，使用高粘性液体的磁性分离磁性纳米粒子的方法清楚地将与细菌组合的磁性纳米粒子与细菌结合的磁性纳米颗粒分离，同时在高粘度液体中显示出带状。 如果使用离心分离器，则纳米颗粒之间的分离能力降低。 此外，根据本发明的方法除了磁性纳米颗粒和磁体之外不需要诸如离心分离器的单独的装置。
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公开(公告)号：KR101523822B1
公开(公告)日：2015-05-28
申请号：KR1020120134544
申请日：2012-11-26
Applicant: 포항공과대학교 산학협력단 , 대한민국 (식품의약품안전처장)
IPC: C12Q1/04 , C12Q1/24 , G01N33/569
CPC classification number: G01N33/54333 , C12Q1/04 , C12Q1/24 , G01N33/54326 , G01N33/54346 , G01N33/56911 , G01N33/56916 , G01N33/587 , G01N2333/255
Abstract: 본발명은식중독균검출방법에관한것으로서, 보다상세하게는음식물등에오염된식중독균의함량을정량적으로신속하게분리할수 있는방법에관한것이다. 상기와같은과제를해결하기위해서, 본발명에따른방법은세균을측정하는시료에상기세균에결합가능한자성나노입자를투입하여세균에상기자성나노입자를결합시키는단계; 자성나노입자를분리하는단계; 자력을이용하여분리된자성나노입자를고점성액체에투과시켜세균이결합된자성나노입자와세균이결합되지않는자성나노입자를분리하는단계; 및세균이결합된자성나노입자를정량하는단계를포함하는것을특징으로한다. 본발명에서와같이, 고점성액체에서자력을이용하여자성나노입자를분리하는방식은세균이결합되지않은자성나노입자가고점성액체에서띠 형태를띠면서세균이결합되는자성나노입자로부터명확하게분리된다는것이다. 원심분리기를이용하는경우는나노입자간의분리능이떨어지는문제가있다. 또한, 본발명에따르는방법은세균과결합할수 있는자성나노입자와자석을제외하고는원심분리기와같은별도의장치를필요로하지않는다는점에서특히유용하다.
Abstract translation: 本发明涉及一种检测食物中毒细菌的方法，更具体地说，涉及一种快速，定量分离污染食物等的食物中毒细菌成分的方法。 根据本发明的方法的特征在于包括以下步骤：将可与细菌结合的磁性纳米颗粒引入用于测量细菌的样品中，以将磁性纳米颗粒结合到细菌上; 分离磁性纳米粒子; 将通过使用磁性分离的纳米颗粒通过具有高粘度的溶液通过，以将与细菌结合的磁性纳米颗粒结合的磁性纳米颗粒分离; 并定量细菌所结合的磁性纳米颗粒。
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4.发明公开고추다대기 혼입 불량 가짜고춧가루의 분자생물학적 판별을 위한 신규 올리고뉴클레오티드 PCR 프라이머 审中-实审新型物种特异性寡核苷酸PCR引物，用于分子生物学检测经济活性成分红色粉末粉末
公开(公告)号：KR1020140132809A
公开(公告)日：2014-11-19
申请号：KR1020130050879
申请日：2013-05-06
Applicant: 전북대학교산학협력단 , 대한민국 (식품의약품안전처장)
CPC classification number: C12N15/11 , C12Q1/6844
Abstract: 본 발명은 마늘 또는 양파 혼입 판별용 프라이머 및 이의 용도에 관한 것으로, 보다 상세하게는 서열번호 1 내지 4로 이루어진 군에서 선택되는 2개 이상의 염기서열을 포함하는 프라이머쌍 및 이를 이용하여 마늘 또는 양파 혼입 여부를 신속하고 정확하며 간단히 판별할 수 있는 방법에 관한 것이다.
Abstract translation: 本发明涉及用于测定大蒜或洋葱掺假的引物及其用途，更具体地，涉及包含选自SEQ ID NO：1至4的核苷酸序列的至少两个的引物对，以及涉及 及时，准确地，简单地确定使用引物对大蒜或洋葱的掺假。
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公开(公告)号：KR101277801B1
公开(公告)日：2013-06-27
申请号：KR1020120059418
申请日：2012-06-01
Applicant: 대한민국 (식품의약품안전처장)
Abstract: PURPOSE: A method for determining the mixture of a root vegetable ingredient using a primer set containing a forward primer and a reverse primer is provided to scientifically monitor foods, and to prevent distribution of bad foods. CONSTITUTION: A method for determining the mixture of a root vegetable ingredient comprises: a step of isolating DNA from a food ingredient; a step of performing first, second, third, and fourth PCR using a primer set; and a step of detecting the presence of the root vegetable ingredient including Panax schinseng, Nopsis lanceolata, Platycodon grandiflorum, and Dioscorea batatas. The primer set is selected from the group consisting of: a primer set comprising a forward primer in sequence number 1 and a reverse primer in sequence number 2 for detecting Panax schinseng; a primer set comprising a forward primer in sequence number 3 and a reverse primer in sequence number 4 for detecting Nopsis lanceolata; a primer set comprising a forward primer in sequence number 5 and a reverse primer in sequence number 6 for detecting Platycodon grandiflorum; and a PCR primer comprising a forward primer in sequence number 7 and a reverse primer in sequence number 8 for detecting Dioscorea batatas.
Abstract translation: 目的：提供一种使用含有正向引物和反向引物的引物组确定根植物成分混合物的方法，以科学监测食物，并防止坏食物的分布。 构成：确定根植物成分的混合物的方法包括：从食品成分中分离DNA的步骤; 使用引物组进行第一，第二，第三和第四PCR的步骤; 以及检测包括人参，Nopsis lanceolata，Platycodon grandiflorum和薯a薯等根系植物成分的存在的步骤。 引物组选自：包含序列号1的正向引物的引物组和序列号2的用于检测人参的反向引物; 包含序列号3的正向引物和序列号4的反向引物的引物组，用于检测花椒（Nopsis lanceolata）; 一种引物组，其包含序列号5的正向引物和序列号6中用于检测桔梗的反向引物; 和包含序列号7的正向引物和序列号8的反向引物的PCR引物，用于检测薯detecting皂甙。
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Patent Agency Ranking
公开(公告)号：KR101277800B1
公开(公告)日：2013-06-27
申请号：KR1020120059417
申请日：2012-06-01
Applicant: 대한민국 (식품의약품안전처장)
Abstract: PURPOSE: A technique for determining the mixture of a mollusks ingredient in a food using a primer set containing a forward primer of a certain sequence and a reverse primer of a certain sequence is provided to enable scientific monitoring of a food, and to prevent distribution of bad food. CONSTITUTION: A method for determining the mixture of a mollusks ingredient in a food comprises: a step of isolating DNA from the food; a step of performing first and second PCR using a primer set and DNA as a template; and a step of detecting the presence of the mollusks ingredient in the food. The primer set is selected from the group consisting of: a primer set containing a forward primer in sequence number 1 and a reverse primer in sequence number 2 for detecting Todarodes pacificus; and a primer set containing a forward primer in sequence number 3 and a reverse primer in sequence number 4 for detecting Loligo bleekeri.
Abstract translation: 目的：提供一种用于使用含有某一序列的正向引物和一定序列的反向引物的引物组确定食品中软体动物成分的混合物的技术，以使科学监测食物，并防止 不好的食物。 构成：确定食品中软体动物成分的混合物的方法包括：从食物中分离DNA的步骤; 使用引物组和DNA作为模板进行第一和第二PCR的步骤; 以及检测食品中软体动物成分的存在的步骤。 引物组选自：包含序列号1的正向引物的引物组和序列号为2的反向引物，用于检测太平洋假丝酵母; 和含有序列号3的正向引物和序列号4的反向引物的引物组，用于检测Loligo bleekeri。
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公开(公告)号：KR101219763B1
公开(公告)日：2013-01-24
申请号：KR1020120059416
申请日：2012-06-01
Applicant: 대한민국 (식품의약품안전처장)
Abstract: PURPOSE: A method for determining the mixture of a fish ingredient in a food ingredient using a PCR prime set is provided to scientifically monitor foods, and to distribute and manage safety foods. CONSTITUTION: A method for determining the mixture of Oreochromis niloticus in a food ingredient comprises a step of using a PCR primer set containing a forward primer of sequence number 7 and a reverse primer of sequence number 8. The PCR primer set for determining the mixture contains the forward and reverse primers.
Abstract translation: 目的：提供一种使用PCR引物确定食品成分中鱼成分混合物的方法，以科学监测食物，并分发和管理安全食品。 构成：用于测定食品成分中的Oreochromis niloticus的混合物的方法包括使用含有序列号7的正向引物和序列号8的反向引物的PCR引物组的步骤。用于测定混合物的PCR引物组含有 正向和反向引物。
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公开(公告)号：KR101445596B1
公开(公告)日：2014-10-07
申请号：KR1020120108074
申请日：2012-09-27
Applicant: 단국대학교 산학협력단 , 대한민국 (식품의약품안전처장)
CPC classification number: G01N33/12 , G01N33/533 , G01N33/54326 , G01N33/552 , G01N33/582
Abstract: 항생제 농도의 측정 방법 및 측정 키트가 제공된다. 본 발명의 일 실시예에 따른 항생제 농도의 측정 방법은, 항생제와 결합된 자성 입자를 준비하는 단계, 항생제의 항체가 하나 이상 결합된 실리카 코팅 형광 입자를 준비하는 단계, 자성 입자를 실리카 코팅 형광 입자와 반응시키는 단계, 및 반응된 실리카 코팅 형광 입자에 레이저를 조사하는 단계를 포함한다.
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公开(公告)号：KR1020140042065A
公开(公告)日：2014-04-07
申请号：KR1020120108074
申请日：2012-09-27
Applicant: 단국대학교 산학협력단 , 대한민국 (식품의약품안전처장)
CPC classification number: G01N33/12 , G01N33/533 , G01N33/54326 , G01N33/552 , G01N33/582
Abstract: Provided are a method and a kit for measuring the concentration of antibiotics. The method for measuring the concentration of antibiotics according to one embodiment of the present invention includes a step of preparing magnetic particles combined with antibiotics, a step of preparing silica coated fluorescent particles combined with one or more antibodies of antibiotics, a step of making the magnetic particles react with the fluorescent particles, and a step of making the silica coated fluorescent particles be irradiated with lasers. [Reference numerals] (AA) Start; (BB) End; (S11) Step of pre-processing antibiotics; (S12) Step of coupling an amine group on the surface of magnetic particles; (S13) Step of adding a cross linking agent to the magnetic particles in which the amine group is combined; (S14) Step of combining the magnetic particles in which the amine group is combined with antibiotics; (S15) Step of collecting the magnetic particles combined with the antibiotics by using magnetic force; (S21) Step of forming coated fluorescent particles by coating the fluorescent particles with silica; (S22) Step of combining a carboxyl group on the surface of silica coated fluorescent particles; (S23) Step of adding the cross linking agent to the silica coated fluorescent particles combined with the carboxyl group; (S24) Step of combining antibodies of the antibiotics and the silica coated fluorescent particles combined with the carboxyl group; (S25) Step of measuring the antibodies amount of the antibiotics combined with the silica coated fluorescent particles; (S3) Step of reacting the magnetic particles with the silica coated fluorescent particles; (S4) Step of collecting the magnetic particles reacting to the silica coated fluorescent particles by using a magnet; (S5) Step of measuring the concentration of the antibiotics by irradiating the silica coated fluorescent particles with a laser
Abstract translation: 提供了用于测量抗生素浓度的方法和试剂盒。 根据本发明的一个实施方案的用于测量抗生素浓度的方法包括制备与抗生素组合的磁性颗粒的步骤，制备与一种或多种抗生素抗体结合的二氧化硅涂覆的荧光颗粒的步骤，制备磁性的步骤 颗粒与荧光颗粒反应，并且用激光照射二氧化硅涂覆的荧光颗粒的步骤。 （附图标记）（AA）开始; （BB）结束; （S11）预处理抗生素的步骤; （S12）将磁性粒子表面上的胺基团偶联的工序; （S13）将交联剂加入其中胺基团的磁性颗粒中的步骤; （S14）将胺基与抗生素组合的磁性粒子组合的工序; （S15）使用磁力收集与抗生素结合的磁性粒子的步骤; （S21）通过用二氧化硅涂布荧光体颗粒来形成涂布的荧光颗粒的步骤; （S22）在二氧化硅被覆荧光体颗粒的表面上组合羧基的工序; （S23）将交联剂添加到与羧基组合的二氧化硅被覆荧光粒子上的工序; （S24）将抗生素的抗体与与羧基结合的二氧化硅被覆荧光粒子结合的步骤; （S25）测定与二氧化硅被覆荧光粒子结合的抗生素的抗体量的步骤; （S3）磁性颗粒与二氧化硅涂覆的荧光颗粒反应的步骤; （S4）使用磁铁收集与二氧化硅涂布的荧光体粒子反应的磁性粒子的工序; （S5）通过用激光照射二氧化硅被覆荧光粒子来测定抗生素浓度的步骤
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公开(公告)号：KR101277798B1
公开(公告)日：2013-07-09
申请号：KR1020120059414
申请日：2012-06-01
Applicant: 대한민국 (식품의약품안전처장)
Abstract: PURPOSE: A method for identifying the mixture of meat ingredients in a food ingredient is provided to enable scientific food monitoring and to block distribution of bad foods. CONSTITUTION: A method for identifying the mixture of meat ingredients in a food ingredient comprises: a step of extracting DNA from the food ingredient; a step of performing PCR comprising first, second, third, fourth, fifth, and sixth steps using the DNA and a primer set; and a step of detecting the presence of meats including bos taurus, sus scrofa, ovis aries, capra hircus, cervus elaphus, cervus Nippon, and equus caballus.
Abstract translation: 目的：提供一种鉴定食品中肉类成分混合物的方法，以便科学的食品监测和阻止不良食品的分销。 构成：用于鉴定食品成分中的肉成分混合物的方法包括：从食品成分中提取DNA的步骤; 使用DNA和引物组进行包含第一，第二，第三，第四，第五和第六步骤的PCR的步骤; 检测肉骨的存在的步骤，包括金牛座，苏格兰黑猩猩，奥们白俄罗斯，披肩，鹿耳鹿，日本母鹿和马鞍马。
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