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公开(公告)号：WO2014081265A1
公开(公告)日：2014-05-30
申请号：PCT/KR2013/010765
申请日：2013-11-26
Applicant: 포항공과대학교 산학협력단 , 대한민국(식품의약품안전처장)
IPC: C12Q1/04 , C12Q1/24 , G01N33/569
CPC classification number: G01N33/54333 , C12Q1/04 , C12Q1/24 , G01N33/54326 , G01N33/54346 , G01N33/56911 , G01N33/56916 , G01N33/587 , G01N2333/255
Abstract: 본 발명은 식중독균 검출 방법에 관한 것으로서, 보다 상세하게는 음식물 등에 오염된 식중독균의 함량을 정량적으로 신속하게 분리할 수 있는 방법에 관한 것이다. 본 발명에 따른 방법은 세균을 측정하는 시료에 상기 세균에 결합 가능한 자성 나노입자를 투입하여 세균에 상기 자성 나노입자를 결합시키는 단계; 자성 나노입자를 분리하는 단계; 자력을 이용하여 분리된 자성 나노입자를 고점성 액체에 투과시켜 세균이 결합된 자성 나노입자와 세균이 결합되지 않는 자성 나노입자를 분리하는 단계; 및 세균이 결합된 자성 나노입자를 정량하는 단계를 포함하는 것을 특징으로 한다.
Abstract translation: 本发明涉及一种检测食物中毒细菌的方法，更具体地，涉及一种快速，定量分离污染食物等的食物中毒细菌成分的方法。 根据本发明的方法的特征在于包括以下步骤：将可与细菌结合的磁性纳米颗粒引入用于测量细菌的样品中，以将磁性纳米颗粒结合到细菌上; 分离磁性纳米粒子; 将通过使用磁性分离的纳米颗粒通过具有高粘度的溶液通过，以将与细菌结合的磁性纳米颗粒结合的磁性纳米颗粒分离; 并定量细菌所结合的磁性纳米颗粒。
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公开(公告)号：KR101523822B1
公开(公告)日：2015-05-28
申请号：KR1020120134544
申请日：2012-11-26
Applicant: 포항공과대학교 산학협력단 , 대한민국 (식품의약품안전처장)
IPC: C12Q1/04 , C12Q1/24 , G01N33/569
CPC classification number: G01N33/54333 , C12Q1/04 , C12Q1/24 , G01N33/54326 , G01N33/54346 , G01N33/56911 , G01N33/56916 , G01N33/587 , G01N2333/255
Abstract: 본발명은식중독균검출방법에관한것으로서, 보다상세하게는음식물등에오염된식중독균의함량을정량적으로신속하게분리할수 있는방법에관한것이다. 상기와같은과제를해결하기위해서, 본발명에따른방법은세균을측정하는시료에상기세균에결합가능한자성나노입자를투입하여세균에상기자성나노입자를결합시키는단계; 자성나노입자를분리하는단계; 자력을이용하여분리된자성나노입자를고점성액체에투과시켜세균이결합된자성나노입자와세균이결합되지않는자성나노입자를분리하는단계; 및세균이결합된자성나노입자를정량하는단계를포함하는것을특징으로한다. 본발명에서와같이, 고점성액체에서자력을이용하여자성나노입자를분리하는방식은세균이결합되지않은자성나노입자가고점성액체에서띠 형태를띠면서세균이결합되는자성나노입자로부터명확하게분리된다는것이다. 원심분리기를이용하는경우는나노입자간의분리능이떨어지는문제가있다. 또한, 본발명에따르는방법은세균과결합할수 있는자성나노입자와자석을제외하고는원심분리기와같은별도의장치를필요로하지않는다는점에서특히유용하다.
Abstract translation: 本发明涉及一种检测食物中毒细菌的方法，更具体地说，涉及一种快速，定量分离污染食物等的食物中毒细菌成分的方法。 根据本发明的方法的特征在于包括以下步骤：将可与细菌结合的磁性纳米颗粒引入用于测量细菌的样品中，以将磁性纳米颗粒结合到细菌上; 分离磁性纳米粒子; 将通过使用磁性分离的纳米颗粒通过具有高粘度的溶液通过，以将与细菌结合的磁性纳米颗粒结合的磁性纳米颗粒分离; 并定量细菌所结合的磁性纳米颗粒。
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公开(公告)号：KR1020140067386A
公开(公告)日：2014-06-05
申请号：KR1020120134544
申请日：2012-11-26
Applicant: 포항공과대학교 산학협력단 , 대한민국 (식품의약품안전처장)
IPC: C12Q1/04 , C12Q1/24 , G01N33/569
CPC classification number: G01N33/54333 , C12Q1/04 , C12Q1/24 , G01N33/54326 , G01N33/54346 , G01N33/56911 , G01N33/56916 , G01N33/587 , G01N2333/255
Abstract: The present invention relates to a method for detecting food borne pathogens and, more specifically, to a method for rapidly and quantitatively isolating contents of food borne pathogens contaminated in foods. In order to solve the assignment, the method according to the present invention comprises the following steps: adding magnetic nanoparticles capable of combining with bacteria to samples which measure the bacteria, and combining the magnetic nanoparticles with the bacteria; isolating the magnetic nanoparticles; separating the magnetic nanoparticles combined with the bacteria from magnetic nanoparticles not combined with the bacteria by transmitting the magnetic nanoparticles isolated using magnetism through a high viscous liquid; and quantifying the magnetic nanoparticles combined with the bacteria. As in the present invention, the method for isolating the magnetic nanoparticles using magnetism from high viscous liquid clearly isolates the magnetic nanoparticles with which bacteria are not combined from the magnetic nanoparticles with which bacteria are combined while showing band shapes in the high viscous liquid. If centrifugal separator is used, separation capability between nanoparticles is decreased. In addition, the method according to the present invention does not require a separate device such as the centrifugal separator, except magnetic nanoparticles and magnets.
Abstract translation: 本发明涉及一种用于检测食源性病原体的方法，更具体地，涉及一种用于快速和定量地分离食品中食源性病原体的内容物的方法。 为了解决这一问题，本发明的方法包括以下步骤：将能够与细菌结合的磁性纳米颗粒加入到测量细菌的样品中，并将磁性纳米颗粒与细菌结合; 分离磁性纳米粒子; 通过传输使用磁性分离的磁性纳米颗粒通过高粘度液体将与细菌结合的磁性纳米颗粒与未与细菌组合的磁性纳米颗粒分离; 并量化与细菌结合的磁性纳米颗粒。 与本发明一样，使用高粘性液体的磁性分离磁性纳米粒子的方法清楚地将与细菌组合的磁性纳米粒子与细菌结合的磁性纳米颗粒分离，同时在高粘度液体中显示出带状。 如果使用离心分离器，则纳米颗粒之间的分离能力降低。 此外，根据本发明的方法除了磁性纳米颗粒和磁体之外不需要诸如离心分离器的单独的装置。
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公开(公告)号：KR101456540B1
公开(公告)日：2014-11-03
申请号：KR1020130051429
申请日：2013-05-07
Applicant: 서울여자대학교 산학협력단 , 대한민국 (식품의약품안전처장)
CPC classification number: B65D65/40 , A01N65/00 , B32B27/08 , B32B2323/04 , B32B2323/10 , B65D81/28
Abstract: 미세캡슐화한 계피 오일을 사용하여 방충효능을 향상시킨 방충 포장재의 제조방법에 관한 것으로, (a) 폴리비닐알코올 수용액을 제조하는 단계, (b) 상기 (a)단계에서 제조된 폴리비닐알코올 수용액에 계피 오일 또는 회향 오일 및 유화제를 첨가한 후 균질화하여 미세캡슐 폴리비닐알코올 에멀젼을 제조하는 단계, (c) 상기 (b)단계에서 제조된 폴리비닐알코올 에멀젼과 잉크를 혼합하여 코팅액을 제조하는 단계, (d) 상기 (c)단계에서 제조된 코팅액을 제1 수지층에 코팅하여 코팅된 제1 수지층을 형성하는 단계를 포함하는 구성을 마련한다.
상기와 같은 제조방법에 의해 제조된 방충 포장재는 화랑곡나방 유충이 기피하는 계피 오일을 미세캡슐화하여 방출물질의 방출 속도를 늦출 수 있어 방충 효능이 탁월하다.Abstract translation: 防虫包装材料的制造方法技术领域本发明涉及防虫包装材料的制造方法，其通过使用微囊化肉桂油来提高害虫防效。 防虫包装材料的制造方法包括：（a）制造聚乙烯醇水溶液的步骤; （b）在步骤（a）中制造的聚乙烯醇水溶液中加入肉桂油或茴香油和乳化剂，制造微胶囊聚乙烯醇乳液的步骤，使其均匀化; （c）通过混合步骤（b）中制造的聚乙烯醇乳液和油墨来制造涂布溶液的步骤; 和（d）通过用步骤（c）中制造的涂布溶液涂覆第一树脂层来形成涂覆的第一树脂层的步骤。 通过制造方法制造的防虫包装材料，通过微囊化肉桂油推迟释放材料的释放速度，从而抵消了印度餐后的幼虫，防虫效果优异。
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5.发明公开유해물질에 대한 데이터를 토대로 인과관계에 기반하여 기후변화에 따른 유해물질 전이 메커니즘을 탐색하는 방법 无效使用危险材料危险性概况的气候变化模型对气候变化的危险材料过渡机制的搜索方法
公开(公告)号：KR1020130078659A
公开(公告)日：2013-07-10
申请号：KR1020110147726
申请日：2011-12-30
Applicant: 한국보건산업진흥원 , 대한민국 (식품의약품안전처장)
CPC classification number: Y02P90/30 , G06Q50/26 , G01W1/02 , G06F17/30241 , G06Q50/04
Abstract: PURPOSE: A method for searching a harmful material transition mechanism corresponding to climate change based on data for harmful materials is provided to accurately guarantee food safety. CONSTITUTION: A specific area, a climate factor, and a temporal range of the climate factor are selected for searching a harmful material transition mechanism corresponding to climate change (S100). A harm factor which is utilized for a search of a transition mechanism is selected (S110). The harm factor is generated by harmful materials. Change amount of the climate factor is calculated in the selected area corresponding to the temporal range, and change amount of the harm factor is calculated after searching a database based on the change amount of the climate factor (S120). Change amount of a dangerous factor is calculated through the change amount of the harm factor (S130). [Reference numerals] (S100) Specific area, a climate factor, and a temporal range of the climate factor are selected for searching a harmful material transition mechanism corresponding to climate change; (S110) Harm factor which is utilized for a search of a transition mechanism is selected; (S120) Change amount of the climate factor is calculated in the selected area corresponding to the temporal range, and change amount of the harm factor is calculated after searching a database based on the change amount of the climate factor; (S130) Change amount of a dangerous factor is calculated through the change amount of the harm factor
Abstract translation: 目的：提供一种基于有害物质数据搜索对应于气候变化的有害物质转换机制的方法，以准确保证食品安全。 构成：选择特定区域，气候因子和气候因子的时间范围，用于搜索对应于气候变化的有害物质转换机制（S100）。 选择用于搜索过渡机制的危害因素（S110）。 危害因素是由有害物质产生的。 在对应于时间范围的选定区域中计算气候因子的变化量，并且基于气候因子的变化量搜索数据库后计算危害因子的变化量（S120）。 通过危害因子的变化量计算危险因素的变化量（S130）。 （附图标记）（S100）选择气候因子的比表面积，气候因子和时间范围来搜索对应于气候变化的有害物质转换机制; （S110）选择用于搜索过渡机制的危害因子; （S120）在与时间范围对应的选择区域中计算气候因子的变化量，根据气候因子的变化量，在搜索数据库后计算危害因子的变化量; （S130）通过危害因子的变化量计算危险因素的变化量
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Patent Agency Ranking
公开(公告)号：KR101277799B1
公开(公告)日：2013-06-27
申请号：KR1020120059415
申请日：2012-06-01
Applicant: 대한민국 (식품의약품안전처장)
Abstract: PURPOSE: A method for determining the mixture of a poultry ingredient in a food using a primer set comprising a forward primer of a certain sequence and a reverse primer of a certain sequence is provided to scientifically monitor foods for prevent distribution of bad foods. CONSTITUTION: A method for determining the mixture of a poultry ingredient in a food comprises: a step of isolating DNA; a step of performing first, second, third, and fourth PCR using a primer set and the DNA as a template; and a step of detecting the poultry ingredient. The primer set is selected from the group consisting of: a primer set comprising a forward primer in sequence number 1 and a reverse primer in sequence number 2 for detecting Gallus gallus; a primer set comprising a forward primer in sequence number 3 and a reverse primer in sequence number 4 for detecting Anas platyrhynchos; a primer set comprising a forward primer in sequence number 5 and a reverse primer in sequence number 6 for detecting Meleagris gallopago; and a primer comprising a forward primer in sequence number 7 and a reverse primer in sequence number 8 for detecting Struthio camelus.
Abstract translation: 目的：提供一种使用包含某一序列的正向引物和一定序列的反向引物的引物组确定食品中家禽成分的混合物的方法，以科学监测食物以防止坏食物的分布。 构成：确定食品中家禽成分混合物的方法包括：分离DNA的步骤; 使用引物组和DNA作为模板进行第一，第二，第三和第四PCR的步骤; 以及检测家禽成分的步骤。 引物组选自：引物组，其包含序列号1的正向引物和序列号为2的反向引物，用于检测Gallus gallus; 包含序列号3的正向引物和序列号4的反向引物的引物组，用于检测Anas platyrhynchos; 序列号5的正向引物和序列号6的反向引物的引物组，用于检测Meleagris gallopago; 和包含序列号7的正向引物和序列号8的反向引物的引物，用于检测骆驼属。
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公开(公告)号：KR101123956B1
公开(公告)日：2012-03-13
申请号：KR1020110007618
申请日：2011-01-26
Applicant: 대한민국 (식품의약품안전처장) , (주)인디텍코리아 , 연세대학교 산학협력단
CPC classification number: G01K11/12 , G01K3/04 , G01K11/06 , G01K2207/04
Abstract: PURPOSE: A critical temperature indicator and a manufacturing method thereof which displays critical temperature of freezing and refrigerated food are provided to make mass production and to display a red sign. CONSTITUTION: A critical temperature indicator comprises a lower part(20), a development medium member(21), a time controller, transparent members(40,45) and an upper part. The upper side is dyed in red or the lower part is formed in the base film. The development medium member absorbs the development material. The time controller adheres to the lower part and development medium member. The transparent member is piled up on the development medium member. The transparent member comprises a first display(42) of linear type. The upper part is composed of the opaque body layer equipped with a molding part.
Abstract translation: 目的：提供显示冷冻和冷冻食品临界温度的临界温度指示器及其制造方法，以便批量生产并显示红色符号。 构成：临界温度指示器包括下部（20），显影介质构件（21），时间控制器，透明构件（40,45）和上部。 上侧染成红色，下部形成在底膜中。 显影介质构件吸收显影材料。 时间控制器粘附到下部和显影介质成员。 透明构件堆积在显影介质构件上。 透明构件包括直线型的第一显示器（42）。 上部由装有成型部的不透明体层构成。
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公开(公告)号：KR1020160109406A
公开(公告)日：2016-09-21
申请号：KR1020150033743
申请日：2015-03-11
Applicant: 창원대학교 산학협력단 , 대한민국 (식품의약품안전처장)
Abstract: 본발명은청소년식생활평가도구와이를이용한식사종류별반정량적식품섭취빈도조사방법에관한것으로, 보다상세하게는청소년식생활평가도구와네트워크, 사용자단말기, 메인서버및 데이터베이스를포함하는식품섭취빈도조사시스템을이용한청소년식품섭취빈도조사방법에있어서, 상기청소년식생활평가도구는아침식사에대한질문, 점심식사에대한질문, 저녁식사에대한질문, 간식에대한질문, 조사결과보기를포함하는안내페이지와조사페이지로구성되고, 상기메인서버는사용자단말기로부터수신되는요청데이터를분석하고, 요청데이터에해당하는청소년식품섭취빈도조사데이터또는청소년식생활평가데이터를데이터베이스를검색하여그 결과를사용자단말기로전송하는단계와; 사용자단말기는메인서버로부터전송되는청소년식품섭취빈도조사데이터또는청소년식생활평가데이터를화면으로출력하는단계를포함한다. 이에따라, 본발명은청소년식품안전영양교육컨텐츠개발과식생활평가도구표준화를통하여청소년의식품안전및 영양안전의인식과실천을향상시켜미래사회의주역인청소년의건강을확보하여건강한국가발전에기여할수 있는매우유용한발명인것이다.
Abstract translation: 本发明涉及一种用于青少年食物寿命的评估工具和用于使用其评估每种膳食种类的半定量营养摄入频率的方法，更具体地，涉及一种使用以下方法测量青少年的营养摄入频率的方法： 用于测量营养摄入频率的系统，其中用于测量营养摄入频率的系统包括用于青少年食物寿命的评估工具，网络，用户终端，主服务器和数据库。 青少年食物生活的评估工具包括指导页面和测量页面，包括关于早餐的询问，午餐的询问，晚餐的查询，关于茶点的查询和检查调查结果。 用于测量营养摄取频率的方法包括：分析从用户终端接收到的请求数据并通过搜索与该请求对应的青少年营养摄入频率或青少年食物寿命评估数据的调查数据向用户终端发送搜索结果的步骤 数据库中的数据库，由主服务器; 以及由用户终端在屏幕上输出关于从主服务器发送的青少年营养摄入频率或青少年食物生活评估数据的调查数据的步骤。 因此，青少年食物生活的评估工具和测量青少年营养摄入量的方法可以通过改善对未来社会的领导作用的青少年的健康，通过改善食物的认识和实践来促进健康的国家发展 通过食品生活评估工具的标准化和青少年食品安全和营养安全教育内容的发展，青少年的安全和营养安全。
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公开(公告)号：KR101445596B1
公开(公告)日：2014-10-07
申请号：KR1020120108074
申请日：2012-09-27
Applicant: 단국대학교 산학협력단 , 대한민국 (식품의약품안전처장)
CPC classification number: G01N33/12 , G01N33/533 , G01N33/54326 , G01N33/552 , G01N33/582
Abstract: 항생제 농도의 측정 방법 및 측정 키트가 제공된다. 본 발명의 일 실시예에 따른 항생제 농도의 측정 방법은, 항생제와 결합된 자성 입자를 준비하는 단계, 항생제의 항체가 하나 이상 결합된 실리카 코팅 형광 입자를 준비하는 단계, 자성 입자를 실리카 코팅 형광 입자와 반응시키는 단계, 및 반응된 실리카 코팅 형광 입자에 레이저를 조사하는 단계를 포함한다.
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公开(公告)号：KR1020140042065A
公开(公告)日：2014-04-07
申请号：KR1020120108074
申请日：2012-09-27
Applicant: 단국대학교 산학협력단 , 대한민국 (식품의약품안전처장)
CPC classification number: G01N33/12 , G01N33/533 , G01N33/54326 , G01N33/552 , G01N33/582
Abstract: Provided are a method and a kit for measuring the concentration of antibiotics. The method for measuring the concentration of antibiotics according to one embodiment of the present invention includes a step of preparing magnetic particles combined with antibiotics, a step of preparing silica coated fluorescent particles combined with one or more antibodies of antibiotics, a step of making the magnetic particles react with the fluorescent particles, and a step of making the silica coated fluorescent particles be irradiated with lasers. [Reference numerals] (AA) Start; (BB) End; (S11) Step of pre-processing antibiotics; (S12) Step of coupling an amine group on the surface of magnetic particles; (S13) Step of adding a cross linking agent to the magnetic particles in which the amine group is combined; (S14) Step of combining the magnetic particles in which the amine group is combined with antibiotics; (S15) Step of collecting the magnetic particles combined with the antibiotics by using magnetic force; (S21) Step of forming coated fluorescent particles by coating the fluorescent particles with silica; (S22) Step of combining a carboxyl group on the surface of silica coated fluorescent particles; (S23) Step of adding the cross linking agent to the silica coated fluorescent particles combined with the carboxyl group; (S24) Step of combining antibodies of the antibiotics and the silica coated fluorescent particles combined with the carboxyl group; (S25) Step of measuring the antibodies amount of the antibiotics combined with the silica coated fluorescent particles; (S3) Step of reacting the magnetic particles with the silica coated fluorescent particles; (S4) Step of collecting the magnetic particles reacting to the silica coated fluorescent particles by using a magnet; (S5) Step of measuring the concentration of the antibiotics by irradiating the silica coated fluorescent particles with a laser
Abstract translation: 提供了用于测量抗生素浓度的方法和试剂盒。 根据本发明的一个实施方案的用于测量抗生素浓度的方法包括制备与抗生素组合的磁性颗粒的步骤，制备与一种或多种抗生素抗体结合的二氧化硅涂覆的荧光颗粒的步骤，制备磁性的步骤 颗粒与荧光颗粒反应，并且用激光照射二氧化硅涂覆的荧光颗粒的步骤。 （附图标记）（AA）开始; （BB）结束; （S11）预处理抗生素的步骤; （S12）将磁性粒子表面上的胺基团偶联的工序; （S13）将交联剂加入其中胺基团的磁性颗粒中的步骤; （S14）将胺基与抗生素组合的磁性粒子组合的工序; （S15）使用磁力收集与抗生素结合的磁性粒子的步骤; （S21）通过用二氧化硅涂布荧光体颗粒来形成涂布的荧光颗粒的步骤; （S22）在二氧化硅被覆荧光体颗粒的表面上组合羧基的工序; （S23）将交联剂添加到与羧基组合的二氧化硅被覆荧光粒子上的工序; （S24）将抗生素的抗体与与羧基结合的二氧化硅被覆荧光粒子结合的步骤; （S25）测定与二氧化硅被覆荧光粒子结合的抗生素的抗体量的步骤; （S3）磁性颗粒与二氧化硅涂覆的荧光颗粒反应的步骤; （S4）使用磁铁收集与二氧化硅涂布的荧光体粒子反应的磁性粒子的工序; （S5）通过用激光照射二氧化硅被覆荧光粒子来测定抗生素浓度的步骤
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