公开(公告)号：KR1020010076994A

公开(公告)日：2001-08-17

申请号：KR1020000004484

申请日：2000-01-29

Applicant: 한국과학기술연구원

Inventor： 유재훈 ,  박호군 ,  윤현주 ,  정선주 ,  최소영 ,  권미윤

IPC: C07H21/00

Abstract: PURPOSE: Provided is DNA sequence that codes variable region of an antibody having enzymatic activity degrading glycosidic bond. And its expression method is also provided. CONSTITUTION: A DNA sequence coding variable region of an antibody having enzymatic activity degrading glycosidic bond is as follows: DNA sequence represented by sequence ID. No. 1 coding VH chain of antibody for glycosidase, 4f4r; DNA sequence represented by sequence ID. No. 3 coding VH chain of antibody for glycosidase, 6h4h; DNA sequence represented by sequence ID. No. 5 coding VH chain of antibody for glycosidase, Ab2l; DNA sequence represented by sequence ID. No. 7 coding antibody for glycosidase, 4f4r; DNA sequence represented by sequence ID. No. 9 coding VL chain of antibody for glycosidase, 6h4h; or DNA sequence represented by sequence ID. No. 11 coding VL chain of antibody for glycosidase, Ab2l. Wherein, all sequences are described as in the description. The antibody is prepared by expressing a fusion protein of VH and VL in an eukaryote cell or bacteria.

Abstract translation: 目的：提供编码具有酶活性降解糖苷键的抗体的可变区的DNA序列。 并提供其表达方法。 构成：具有酶活性降解糖苷键的抗体的编码可变区的DNA序列如下：由序列ID表示的DNA序列。 1号编码糖苷酶抗体的VH链，4f4r; 由序列ID表示的DNA序列。 3号编码糖苷酶抗体VH链，6h4h; 由序列ID表示的DNA序列。 编码糖基化抗体的第5号VH链，Ab2l; 由序列ID表示的DNA序列。 糖苷酶7号编码抗体，4f4r; 由序列ID表示的DNA序列。 9号编码糖苷酶抗体VL链，6h4h; 或由序列ID表示的DNA序列。 11号编码糖苷酶抗体的VL链，Ab2l。 其中所有序列如描述中所述。 通过在真核细胞或细菌中表达VH和VL的融合蛋白来制备抗体。