公开(公告)号：KR1020010076994A

公开(公告)日：2001-08-17

申请号：KR1020000004484

申请日：2000-01-29

Applicant: 한국과학기술연구원

Inventor： 유재훈 ,  박호군 ,  윤현주 ,  정선주 ,  최소영 ,  권미윤

IPC: C07H21/00

Abstract: PURPOSE: Provided is DNA sequence that codes variable region of an antibody having enzymatic activity degrading glycosidic bond. And its expression method is also provided. CONSTITUTION: A DNA sequence coding variable region of an antibody having enzymatic activity degrading glycosidic bond is as follows: DNA sequence represented by sequence ID. No. 1 coding VH chain of antibody for glycosidase, 4f4r; DNA sequence represented by sequence ID. No. 3 coding VH chain of antibody for glycosidase, 6h4h; DNA sequence represented by sequence ID. No. 5 coding VH chain of antibody for glycosidase, Ab2l; DNA sequence represented by sequence ID. No. 7 coding antibody for glycosidase, 4f4r; DNA sequence represented by sequence ID. No. 9 coding VL chain of antibody for glycosidase, 6h4h; or DNA sequence represented by sequence ID. No. 11 coding VL chain of antibody for glycosidase, Ab2l. Wherein, all sequences are described as in the description. The antibody is prepared by expressing a fusion protein of VH and VL in an eukaryote cell or bacteria.

Abstract translation: 目的：提供编码具有酶活性降解糖苷键的抗体的可变区的DNA序列。 并提供其表达方法。 构成：具有酶活性降解糖苷键的抗体的编码可变区的DNA序列如下：由序列ID表示的DNA序列。 1号编码糖苷酶抗体的VH链，4f4r; 由序列ID表示的DNA序列。 3号编码糖苷酶抗体VH链，6h4h; 由序列ID表示的DNA序列。 编码糖基化抗体的第5号VH链，Ab2l; 由序列ID表示的DNA序列。 糖苷酶7号编码抗体，4f4r; 由序列ID表示的DNA序列。 9号编码糖苷酶抗体VL链，6h4h; 或由序列ID表示的DNA序列。 11号编码糖苷酶抗体的VL链，Ab2l。 其中所有序列如描述中所述。 通过在真核细胞或细菌中表达VH和VL的融合蛋白来制备抗体。

公开(公告)号：KR100439736B1

公开(公告)日：2004-07-12

申请号：KR1020010030455

申请日：2001-05-31

Applicant: 한국과학기술연구원

Inventor： 유재훈 ,  권미윤

IPC: C12Q1/68

Abstract: PURPOSE: Provided is a method of selecting new target mRNA from mRNA libraries by using affinity selection to identify new mRNA easily and rapidly by using human genome gene. CONSTITUTION: The method of selecting new target mRNA from mRNA libraries by using affinity selection comprises the steps of: selecting mRNAs having specific affinity to a target compound from an RNA library using SELEX; and conforming mRNA specific to an abnormal cell by hybridizing the selected mRNAs with normal cell DNA or on a DNA chip where normal cell cDNA.

Abstract translation: 目的：通过使用人类基因组基因，通过使用亲和选择来容易且迅速地鉴定新mRNA，从mRNA文库中选择新的靶mRNA。 组成：通过使用亲和选择从mRNA文库中选择新的靶mRNA的方法包括以下步骤：使用SELEX从RNA文库中选择对目标化合物具有特异性亲和力的mRNA; 通过将选择的mRNA与正常细胞DNA杂交或在正常细胞cDNA的DNA芯片上杂交，使特定于异常细胞的mRNA相一致。

公开(公告)号：KR100450864B1

公开(公告)日：2004-10-01

申请号：KR1020010073358

申请日：2001-11-23

Applicant: 한국과학기술연구원

Inventor： 유재훈 ,  이종국 ,  권미윤 ,  신계정

IPC: C07H15/232

CPC classification number: C07H5/10 ,  C07H15/232

Abstract: The present invention relates to heterodimeric conjugates of neomycin-chloramphenicol, of formula 1, their preparation and their use. Because of their heterodmieric structure, they can recognize both stem and loop of RNA motif and show binding ability to a certain RNA such that they have an enhanced pharmaceutical efficacy and reduced side effect which can be caused by non-specific drugs. For these reasons, they can be effectively used as an antiviral agent, an antibacterial agent or an anticancer drugs.

Abstract translation: 本发明涉及式1的新霉素 - 氯霉素的异二聚体缀合物，它们的制备及其用途。 由于它们具有异源性结构，因此它们可以识别RNA基序的茎和环，并显示对某种RNA的结合能力，从而具有增强的药效和减少可能由非特异性药物引起的副作用。 由于这些原因，它们可以有效地用作抗病毒剂，抗菌剂或抗癌药物。

公开(公告)号：KR100344273B1

公开(公告)日：2002-07-24

申请号：KR1020000004319

申请日：2000-01-28

Applicant: 한국과학기술연구원

Inventor： 유재훈 ,  권미윤

IPC: C07K7/00

Abstract: 본발명은 생체내 세포간 신호 전달, 또는 병원체의 숙주세포 인식에 중요한 sLeX라는 4당류에 접합하는 펩타이드분자, 및 상기 펩타이드를 연속적인 모티브로 가지며 시알릴 루이스 엑스에 특이적으로 결합하는 단백질을 찾는데 상기 펩타이드분자를 사용하는 방법을 제공한다. 당을 인식하는 일열구조의 펩타이드 반복 모티프는 여러 가지 병원체의 감염억제, 백신의 개발, 또는 당-표적자 인식 분자로서, 효과적인 치료제로 이용할 수 있다.

公开(公告)号：KR1020030042632A

公开(公告)日：2003-06-02

申请号：KR1020010073358

申请日：2001-11-23

Applicant: 한국과학기술연구원

Inventor： 유재훈 ,  이종국 ,  권미윤 ,  신계정

IPC: C07H15/232

CPC classification number: C07H5/10 ,  C07H15/232

Abstract: PURPOSE: Provided is a heterodimer of neomycin and chloramphenicol with improved specificity to a target RNA molecule to increase the medicinal efficacy while decreasing adverse effects due to the non-specific drugs. It is effectively used as an anti-virus agent, an anti-bacteria agent, or an anti-cancer agent. CONSTITUTION: A heterodimer(formula 1) of neomycin and chloramphenicol is structured such that the heterodimer is specific-coupled to 16S rRNA, RRE RNA, or TS RNA. In the formula(1), n is an integer of 2-10 and, more particularly, 3, 6 or 9. The specific coupling of the heterodimer to the RNA is characterized by simultaneously recognizing the stem and the loop of the RNA. The specific coupling is intrinsic to the sequence of RNA.

Abstract translation: 目的：提供新霉素和氯霉素的异二聚体，对目标RNA分子具有改进的特异性，以增加药物功效，同时减少由非特异性药物引起的不良反应。 它有效地用作抗病毒剂，抗菌剂或抗癌剂。 构成：新霉素和氯霉素的异二聚体（配方1）的结构使得异二聚体与16S rRNA，RRE RNA或TS RNA特异性偶联。 在式（1）中，n是2-10的整数，更特别是3-6或9.异源二聚体与RNA的特异性偶联特征在于同时识别RNA的茎和环。 特异性偶联是RNA序列固有的。

公开(公告)号：KR100445437B1

公开(公告)日：2004-08-21

申请号：KR1020020007495

申请日：2002-02-08

Applicant: 한국과학기술연구원

Inventor： 유재훈 ,  이종국 ,  권미윤 ,  배애님 ,  고훈영

IPC: C07H15/22

CPC classification number: C07H15/232

Abstract: The present invention relates to novel oxazolidinone derivatives represented as following compound I and a process for the preparation thereof. The compounds of the present invention have wide antibacterial spectrums superior antibacterial activity and low toxicity. Therefore, it can be expected to use as novel antibacterial agent. wherein, R 1 is alkylcarboxyl group or -CH 2 R 2 (wherein, R 2 is OH, argido group, -OR 3 (wherein, R 3 is C 1-4 alkyl, methansulfonyl, p-toluensulfonyl, carboxyl, C 1-4 alkylcarboxyl, C 1-4 alkylcarbonyl, benzyloxycarbonyl, or imidazolylcarbonyl), or -NHR 4 ).

Abstract translation: 本发明涉及下述化合物I所代表的新型恶唑烷酮衍生物及其制备方法。 本发明的化合物具有优异的抗菌活性和低毒性的广谱抗菌谱。 因此，可以期待用作新型抗菌剂。 其中R SUB 1 1是烷基羧基或-CH 2 SUB 2 R SUB 2 SUB（其中，R SUB 2 2 SUB是 OH，Argido基团，-OR SUB 3（其中，R SUB 3是SUB 1-4烷基，甲磺酰基，对甲苯磺酰基， 羧基，C 1-4亚烷基羧基，C 1-4亚烷基羰基，苄氧基羰基或咪唑基羰基）或-NHR SUB 4 / SUB）。

公开(公告)号：KR100428469B1

公开(公告)日：2004-04-27

申请号：KR1020010020421

申请日：2001-04-17

Applicant: 한국과학기술연구원

Inventor： 유재훈 ,  권미윤 ,  정선주

IPC: C07K7/08

CPC classification number: C07K7/08

Abstract: The present invention relates to carbohydrate-specific peptides, which have a strong affinity and preparation method thereof. Particularly, the present invention relates to carbohydrate-specific peptides, which have been developed to have an increased strong affinity to carbohydrates by the multiple combinations of peptides having carbohydrates-specific amino acid sequences and preparation method thereof. Carbohydrate-specific peptides of the present invention have a very strong affinity under micromol, so that they can shut off recognition between cells transferred by specific carbohydrates, which means they can be used for the treatment of diseases mediated by recognition between cells and used as a diagnostic kit which diagnoses diseases by confirming the existence of any specific carbohydrate.

Abstract translation: 本发明涉及具有强亲和力的碳水化合物特异性肽及其制备方法。 特别地，本发明涉及通过具有碳水化合物特异性氨基酸序列的肽的多种组合具有增强的对碳水化合物的强亲和力的碳水化合物特异性肽及其制备方法。 本发明的碳水化合物特异性肽在微摩尔下具有非常强的亲和力，从而它们可以关闭由特定碳水化合物转移的细胞之间的识别，这意味着它们可以用于治疗由细胞间识别介导的疾病并用作 通过确认任何特定碳水化合物的存在来诊断疾病的诊断试剂盒。

公开(公告)号：KR1020030067355A

公开(公告)日：2003-08-14

申请号：KR1020020007495

申请日：2002-02-08

Applicant: 한국과학기술연구원

Inventor： 유재훈 ,  이종국 ,  권미윤 ,  배애님 ,  고훈영

IPC: C07H15/22

CPC classification number: C07H15/232

Abstract: PURPOSE: Provided is a heterodimeric conjugate of neomycin-oxazolidinone which is useful as an antiviral agent and an antibacterial agent. Also, provided are the preparation method and use thereof. CONSTITUTION: A heterodimeric conjugate of neomycin-oxazolidinone is represented by the formula(1), wherein n is an integer of 2-10 and Ac represents an acetyl group. It is characterized by specifically bonding to 16S rRNA, RRE, RNA or 23S rRNA.

Abstract translation: 目的：提供新霉素 - 恶唑烷酮的异二聚体缀合物，其可用作抗病毒剂和抗菌剂。 此外，提供其制备方法和用途。 构成：新霉素 - 恶唑烷酮的异二聚体缀合物由式（1）表示，其中n为2-10的整数，Ac为乙酰基。 其特征是特异性结合16S rRNA，RRE，RNA或23S rRNA。

公开(公告)号：KR1020020091627A

公开(公告)日：2002-12-06

申请号：KR1020010030455

申请日：2001-05-31

Applicant: 한국과학기술연구원

Inventor： 유재훈 ,  권미윤

IPC: C12Q1/68

Abstract: PURPOSE: Provided is a method of selecting new target mRNA from mRNA libraries by using affinity selection to identify new mRNA easily and rapidly by using human genome gene. CONSTITUTION: The method of selecting new target mRNA from mRNA libraries by using affinity selection comprises the steps of: selecting mRNAs having specific affinity to a target compound from an RNA library using SELEX; and conforming mRNA specific to an abnormal cell by hybridizing the selected mRNAs with normal cell DNA or on a DNA chip where normal cell cDNA.

Abstract translation: 目的：提供一种通过使用亲和力选择，通过使用人类基因组基因容易且快速地鉴定新的mRNA从mRNA文库中选择新的靶mRNA的方法。 构成：通过使用亲和力选择从mRNA文库中选择新的靶mRNA的方法包括以下步骤：使用SELEX从RNA文库中选择对目标化合物具有特异亲和力的mRNA; 并通过将所选择的mRNA与正常细胞DNA或正常细胞cDNA的DNA芯片杂交，使异常细胞特异性的mRNA符合。

公开(公告)号：KR1020020080691A

公开(公告)日：2002-10-26

申请号：KR1020010020421

申请日：2001-04-17

Applicant: 한국과학기술연구원

Inventor： 유재훈 ,  권미윤 ,  정선주

IPC: C07K7/08

CPC classification number: C07K7/08

Abstract: PURPOSE: Provided are carbohydrate-specific peptides which have a strong affinity and a preparation method thereof. In particular, the carbohydrate-specific peptides are prepared by the multiple bond of peptides having a carbohydrate-specific sequence to increase an affinity. CONSTITUTION: The carbohydrate-specific peptides which have a strong affinity is characteristically prepared by the steps of; finding out peptides, which bind to carbohydrates, on the surface of a cell; and preparing peptides having a strong affinity to carbohydrates being on the surface of a cell by manufacturing a polymer of the peptides, wherein the polymer is manufactured by linking an amino acid having at least two amino residues to a peptide.

Abstract translation: 目的：提供具有强亲和力的碳水化合物特异性肽及其制备方法。 特别地，通过具有碳水化合物特异性序列的肽的多重键来制备碳水化合物特异性肽以增加亲和力。 构成：具有强亲和力的碳水化合物特异性肽通过以下步骤特征性地制备： 在细胞表面发现结合碳水化合物的肽; 并且通过制备肽的聚合物制备对碳水化合物具有强亲和力的肽，其中所述聚合物通过将具有至少两个氨基残基的氨基酸连接到肽来制备。