公开(公告)号：AU2567888A

公开(公告)日：1989-05-25

申请号：AU2567888

申请日：1988-11-17

Applicant: ABBOTT LAB

Inventor： SARIN VIRENDER K ,  KNIGGE KEVIN M

IPC: C07K14/705 ,  C07K14/00 ,  C07K14/155 ,  C07K14/16 ,  C07K16/10 ,  C07K19/00 ,  C12N7/04 ,  C12N15/02 ,  C12P21/02 ,  C12P21/08 ,  G01N33/569 ,  C07K13/00 ,  G01N33/571 ,  C07K7/10 ,  C07K15/12 ,  G01N33/545 ,  G01N33/577 ,  C12N15/00 ,  A61K37/02 ,  A61K39/21

Abstract: Novel synthetic peptides which substantially replicate various sequences of gp160 envelope protein of the human immunodeficiency virus (HIV) are disclosed. Also disclosed are methods for detection of antibodies to HIV in biological samples using the gp160 peptides.

公开(公告)号：ES2099699T3

公开(公告)日：1997-06-01

申请号：ES90108317

申请日：1990-05-02

Applicant: ABBOTT LAB

Inventor： POPE MARK R ,  KNIGGE KEVIN M

IPC: G01N33/547 ,  G01N33/543 ,  G01N33/569

Abstract: The present invention provides novel methods for the covalent attachment of a specific binding member to a solid phase through the reaction of a thiolated solid phase and a specific binding member that is complexed to a heterobifunctional coupling agent. The resulting immobilized specific binding member can be used in diagnostic binding assays. The invention enables the production of immobilized specific binding members which have increased sensitivity, specificity and stability, while requiring less specific binding member be used.

公开(公告)号：AU8174694A

公开(公告)日：1995-03-16

申请号：AU8174694

申请日：1994-12-23

Applicant: ABBOTT LAB

Inventor： CHAN EMERSON W ,  ROBEY WILLIAM GERARD ,  DALUGA CYNTHIA K ,  KNIGGE KEVIN M ,  STOJAK JOSEPH J II ,  SCHULZE WERNER ,  BRAUN BRIAN P ,  KAPSALIS ANDREAS A ,  STEPHENS JOHN E ,  VALLARI DAVID S

IPC: G01N33/543 ,  G01N33/569 ,  G01N33/68 ,  G01N33/571 ,  G01N33/576

Abstract: An immunoassay which is capable of simultaneously detecting any desired number of antigens of one infectious agent, or combinations of antigens of several infectious agents, or any desired number of immunoglobulins of one infectious agent, or combinations of immunoglobulins of several infectious agents on a single solid support. A test sample is contacted with a solid support on which one or more antigens are immobilized as discrete test sites. Antigen-antibody complexes are formed and detected on the solid support.

公开(公告)号：DE3687276T2

公开(公告)日：1993-05-06

申请号：DE3687276

申请日：1986-10-03

Applicant: ABBOTT LAB

Inventor： BROWN III ,  DEVEREAUX SHARON M ,  KNIGGE KEVIN M ,  CLEMENS JOHN M ,  HOFLER JOHN G ,  SAFFORD SARAH E

IPC: G01N33/548 ,  G01N31/22 ,  G01N33/52 ,  G01N33/543 ,  G01N33/546 ,  G01N33/569 ,  G01N33/576 ,  G01N33/76

Abstract: A novel material and device useful in solid-phase binding assays to determine the presence or amount of an analyte in a test sample, particularly antigens or antibodies, is disclosed. The material comprises a porous matrix of fibers and a plurality of substantially spherical, solid particles having an average diameter of from about 0.1 to about 5 microns. The particles are retained and immobilized upon the fibers of the matrix. Preferably, the particles have on their surfaces a substance capable of reaction with the analyte in the sample, and the average diameter of the particles is less than the average pore size of the matrix. The device, in a preferred embodiment, comprises a substantially planar layer of the described material.

公开(公告)号：DE3686116D1

公开(公告)日：1992-08-27

申请号：DE3686116

申请日：1986-10-03

Applicant: ABBOTT LAB

Inventor： BROWN III WILLIAM E ,  DEVEREAUX SHARON M ,  KNIGGE KEVIN M ,  CLEMENS JOHN M ,  HOFLER JOHN G ,  SAFFORD SARAH E

IPC: G01N33/548 ,  G01N31/22 ,  G01N33/52 ,  G01N33/543 ,  G01N33/546 ,  G01N33/569 ,  G01N33/576 ,  G01N33/76

Abstract: A novel material and device useful in solid-phase binding assays to determine the presence or amount of an analyte in a test sample, particularly antigens or antibodies, is disclosed. The material comprises a porous matrix of fibers and a plurality of substantially spherical, solid particles having an average diameter of from about 0.1 to about 5 microns. The particles are retained and immobilized upon the fibers of the matrix. Preferably, the particles have on their surfaces a substance capable of reaction with the analyte in the sample, and the average diameter of the particles is less than the average pore size of the matrix. The device, in a preferred embodiment, comprises a substantially planar layer of the described material.

公开(公告)号：CA1301648C

公开(公告)日：1992-05-26

申请号：CA615951

申请日：1990-12-06

Applicant: ABBOTT LAB

Inventor： BROWN WILLIAM E III ,  CLEMENS JOHN M ,  DEVEREAUX SHARON M ,  HOFLER JOHN G ,  KNIGGE KEVIN M ,  SAFFORD SARAH E

IPC: G01N33/548 ,  G01N31/22 ,  G01N33/52 ,  G01N33/543 ,  G01N33/546 ,  G01N33/569 ,  G01N33/576 ,  G01N33/76 ,  C12M1/34

Abstract: A novel material and device useful in solid-phase binding assays to determine the presence or amount of an analyte in a test sample, particularly antigens or antibodies, is disclosed. The material comprises a porous matrix of fibers and a plurality of substantially spherical, solid particles having an average diameter of from about 0.1 to about 5 microns. The particles are retained and immobilized upon the fibers of the matrix. Preferably, the particles have on their surfaces a substance capable of reaction with the analyte in the sample, and the average diameter of the particles is less than the average pore size of the matrix. The device, in a preferred embodiment, comprises a substantially planar layer of the described material.

公开(公告)号：CA2043689A1

公开(公告)日：1991-12-05

申请号：CA2043689

申请日：1991-05-31

Applicant: ABBOTT LAB

Inventor： CHAN EMERSON W ,  SCHULZE WERNER ,  ROBEY WILLIAM G ,  BRAUN BRIAN P ,  DALUGA CYNTHIA K ,  KAPSALIS ANDREAS A ,  KNIGGE KEVIN M ,  STEPHENS JOHN E ,  STOJAK JOSEPH J II ,  VALLARI DAVID S

IPC: G01N33/543 ,  G01N33/569

Abstract: An immunoassay which is capable of simultaneously detecting any desired number of antigens of one infectious agent, or combinations of antigens of several infectious agents, or any desired number of immunoglobulins of one infectious agent, or combinations of immunoglobulins of several infectious agents on a single solid support. A test sample is contacted with a solid support on which one or more antigens are immobilized as discrete test sites. Antigen-antibody complexes are formed and detected on the solid support.

公开(公告)号：DE69029873T2

公开(公告)日：1997-08-07

申请号：DE69029873

申请日：1990-05-02

Applicant: ABBOTT LAB

Inventor： POPE MARK R ,  KNIGGE KEVIN M

IPC: G01N33/547 ,  G01N33/543 ,  G01N33/569

Abstract: The present invention provides novel methods for the covalent attachment of a specific binding member to a solid phase through the reaction of a thiolated solid phase and a specific binding member that is complexed to a heterobifunctional coupling agent. The resulting immobilized specific binding member can be used in diagnostic binding assays. The invention enables the production of immobilized specific binding members which have increased sensitivity, specificity and stability, while requiring less specific binding member be used.

公开(公告)号：DE3686116T2

公开(公告)日：1993-01-28

申请号：DE3686116

申请日：1986-10-03

Applicant: ABBOTT LAB

Inventor： BROWN III WILLIAM E ,  DEVEREAUX SHARON M ,  KNIGGE KEVIN M ,  CLEMENS JOHN M ,  HOFLER JOHN G ,  SAFFORD SARAH E

IPC: G01N33/548 ,  G01N31/22 ,  G01N33/52 ,  G01N33/543 ,  G01N33/546 ,  G01N33/569 ,  G01N33/576 ,  G01N33/76

Abstract: A novel material and device useful in solid-phase binding assays to determine the presence or amount of an analyte in a test sample, particularly antigens or antibodies, is disclosed. The material comprises a porous matrix of fibers and a plurality of substantially spherical, solid particles having an average diameter of from about 0.1 to about 5 microns. The particles are retained and immobilized upon the fibers of the matrix. Preferably, the particles have on their surfaces a substance capable of reaction with the analyte in the sample, and the average diameter of the particles is less than the average pore size of the matrix. The device, in a preferred embodiment, comprises a substantially planar layer of the described material.

公开(公告)号：AT83325T

公开(公告)日：1992-12-15

申请号：AT90109680

申请日：1990-05-22

Applicant: ABBOTT LAB

Inventor： BROWN WILLIAM E III ,  DEVEREAUX SHARON M ,  KNIGGE KEVIN M ,  CLEMENS JOHN M ,  HOFLER JOHN G ,  SAFFORD SARAH E

IPC: G01N33/548 ,  G01N31/22 ,  G01N33/52 ,  G01N33/543 ,  G01N33/546 ,  G01N33/569 ,  G01N33/576 ,  G01N33/76

Abstract: A novel material and device useful in solid-phase binding assays to determine the presence or amount of an analyte in a test sample, particularly antigens or antibodies, is disclosed. The material comprises a porous matrix of fibers and a plurality of substantially spherical, solid particles having an average diameter of from about 0.1 to about 5 microns. The particles are retained and immobilized upon the fibers of the matrix. Preferably, the particles have on their surfaces a substance capable of reaction with the analyte in the sample, and the average diameter of the particles is less than the average pore size of the matrix. The device, in a preferred embodiment, comprises a substantially planar layer of the described material.