公开(公告)号：DE68927118D1

公开(公告)日：1996-10-17

申请号：DE68927118

申请日：1989-01-25

Applicant: ABBOTT LAB

Inventor： HILTIBRAN ROBERT G ,  JOU YI-HER ,  KLINE STEVEN J ,  SCHULTZ STEVEN GEORGE ,  STROUPE STEPHEN DENHAM

IPC: G01N33/532 ,  G01N33/538 ,  G01N33/543 ,  G01N33/574 ,  G01N33/537

Abstract: This invention presents novel separation and assay procedures which allows both the indicator and the capture reagents to be in solution to avoid problems of slowed immunoreaction kinetics. The separation procedure involves an analyte-specific soluble capture reagent, that is conjugated to a charged substance, and an insoluble solid phase material that is oppositely charged. A fluid sample suspected of containing the analyte is mixed with the capture reagent in solution to form a charged capture reagent/analyte complex. When binding is complete, the solution is contacted to the oppositely charged solid phase material to attract, attach, and separate the capture reagent/analyte complex from the fluid sample. With the appropriate indicator reagent, i.e., a second analyte-specific binding substance which is conjugated to a label capable of producing a detectable signal, both sandwich and competitive assays can be performed. The assay reaction complex can be separated from the solution by contact with the oppositely charged solid phase material, and the presence or amount of analyte is monitored by detecting the label of the indicator reagent.

公开(公告)号：AU2866089A

公开(公告)日：1989-08-03

申请号：AU2866089

申请日：1989-01-20

Applicant: ABBOTT LAB

Inventor： HILTIBRAN ROBERT G ,  JOU YI-HER ,  KLINE STEVEN J ,  SCHULTZ STEVEN GEORGE ,  STROUPE STEPHEN DENHAM

IPC: G01N33/532 ,  G01N33/538 ,  G01N33/543 ,  G01N33/574 ,  G01N33/68 ,  C07K17/00 ,  G01N33/74 ,  G01N33/76

Abstract: This invention presents novel separation and assay procedures which allows both the indicator and the capture reagents to be in solution to avoid problems of slowed immunoreaction kinetics. The separation procedure involves an analyte-specific soluble capture reagent, that is conjugated to a charged substance, and an insoluble solid phase material that is oppositely charged. A fluid sample suspected of containing the analyte is mixed with the capture reagent in solution to form a charged capture reagent/analyte complex. When binding is complete, the solution is contacted to the oppositely charged solid phase material to attract, attach, and separate the capture reagent/analyte complex from the fluid sample. With the appropriate indicator reagent, i.e., a second analyte-specific binding substance which is conjugated to a label capable of producing a detectable signal, both sandwich and competitive assays can be performed. The assay reaction complex can be separated from the solution by contact with the oppositely charged solid phase material, and the presence or amount of analyte is monitored by detecting the label of the indicator reagent.

公开(公告)号：DE3686184D1

公开(公告)日：1992-09-03

申请号：DE3686184

申请日：1986-03-10

Applicant: ABBOTT LAB

Inventor： SCHULTZ STEVEN GEORGE

IPC: G01J3/10 ,  G01J3/02 ,  G01J3/04 ,  G01J3/28 ,  G01N21/07 ,  H01L31/0216 ,  G01J3/36 ,  G01J3/00 ,  G01J3/42 ,  G01N21/31 ,  H01L27/14

Abstract: An improved spectrophotometer, especially suitable for use in centrifugal analysis instrumentation, is disclosed. The spectrophotometer is improved by including therein a detector comprising a photodiode array assembly having a photodiode array, a spectral filter assembly situated substantially parallel thereto and in the path of incident light and means for attenuating stray light which would otherwise impinge on each of the photodiodes of the array.

公开(公告)号：ES2016070T3

公开(公告)日：1997-01-16

申请号：ES89101263

申请日：1989-01-25

Applicant: ABBOTT LAB

Inventor： HILTIBRAN ROBERT G ,  JOU YI-HER ,  KLINE STEVEN J ,  SCHULTZ STEVEN GEORGE ,  STROUPE STEPHEN DENHAM

IPC: G01N33/532 ,  G01N33/538 ,  G01N33/543 ,  G01N33/574 ,  G01N33/537

Abstract: This invention presents novel separation and assay procedures which allows both the indicator and the capture reagents to be in solution to avoid problems of slowed immunoreaction kinetics. The separation procedure involves an analyte-specific soluble capture reagent, that is conjugated to a charged substance, and an insoluble solid phase material that is oppositely charged. A fluid sample suspected of containing the analyte is mixed with the capture reagent in solution to form a charged capture reagent/analyte complex. When binding is complete, the solution is contacted to the oppositely charged solid phase material to attract, attach, and separate the capture reagent/analyte complex from the fluid sample. With the appropriate indicator reagent, i.e., a second analyte-specific binding substance which is conjugated to a label capable of producing a detectable signal, both sandwich and competitive assays can be performed. The assay reaction complex can be separated from the solution by contact with the oppositely charged solid phase material, and the presence or amount of analyte is monitored by detecting the label of the indicator reagent.

公开(公告)号：AU609241B2

公开(公告)日：1991-04-26

申请号：AU2866089

申请日：1989-01-20

Applicant: ABBOTT LAB

Inventor： HILTIBRAN ROBERT G ,  JOU YI-HER ,  KLINE STEVEN J ,  SCHULTZ STEVEN GEORGE ,  STROUPE STEPHEN DENHAM

IPC: G01N33/532 ,  G01N33/538 ,  G01N33/543 ,  G01N33/574 ,  G01N33/68 ,  C07K17/00 ,  G01N33/74 ,  G01N33/76

Abstract: This invention presents novel separation and assay procedures which allows both the indicator and the capture reagents to be in solution to avoid problems of slowed immunoreaction kinetics. The separation procedure involves an analyte-specific soluble capture reagent, that is conjugated to a charged substance, and an insoluble solid phase material that is oppositely charged. A fluid sample suspected of containing the analyte is mixed with the capture reagent in solution to form a charged capture reagent/analyte complex. When binding is complete, the solution is contacted to the oppositely charged solid phase material to attract, attach, and separate the capture reagent/analyte complex from the fluid sample. With the appropriate indicator reagent, i.e., a second analyte-specific binding substance which is conjugated to a label capable of producing a detectable signal, both sandwich and competitive assays can be performed. The assay reaction complex can be separated from the solution by contact with the oppositely charged solid phase material, and the presence or amount of analyte is monitored by detecting the label of the indicator reagent.

公开(公告)号：DE68927118T2

公开(公告)日：1997-04-10

申请号：DE68927118

申请日：1989-01-25

Applicant: ABBOTT LAB

Inventor： HILTIBRAN ROBERT G ,  JOU YI-HER ,  KLINE STEVEN J ,  SCHULTZ STEVEN GEORGE ,  STROUPE STEPHEN DENHAM

IPC: G01N33/532 ,  G01N33/538 ,  G01N33/543 ,  G01N33/574 ,  G01N33/537

Abstract: This invention presents novel separation and assay procedures which allows both the indicator and the capture reagents to be in solution to avoid problems of slowed immunoreaction kinetics. The separation procedure involves an analyte-specific soluble capture reagent, that is conjugated to a charged substance, and an insoluble solid phase material that is oppositely charged. A fluid sample suspected of containing the analyte is mixed with the capture reagent in solution to form a charged capture reagent/analyte complex. When binding is complete, the solution is contacted to the oppositely charged solid phase material to attract, attach, and separate the capture reagent/analyte complex from the fluid sample. With the appropriate indicator reagent, i.e., a second analyte-specific binding substance which is conjugated to a label capable of producing a detectable signal, both sandwich and competitive assays can be performed. The assay reaction complex can be separated from the solution by contact with the oppositely charged solid phase material, and the presence or amount of analyte is monitored by detecting the label of the indicator reagent.

公开(公告)号：CA1338928C

公开(公告)日：1997-02-25

申请号：CA589397

申请日：1989-01-27

Applicant: ABBOTT LAB

Inventor： HILTIBRAN ROBERT G ,  JOU YI-HER ,  KLINE STEVEN J ,  SCHULTZ STEVEN GEORGE ,  STROUPE STEPHEN DENHAM

IPC: G01N33/532 ,  G01N33/538 ,  G01N33/543 ,  G01N33/574 ,  G01N33/544

Abstract: This invention presents novel separation and assay procedures which allows both the indicator and the capture reagents to be in solution to avoid problems of slowedimmunoreaction kinetics. The separation procedure involves an analyte-specific soluble capture reagent, that is conjugated to a charged substance, and an insoluble solid phase material that is oppositely charged. A fluid sample suspected of containing the analyte is mixed with the capture reagent in solution to form a charged capture reagent/analyte complex. When binding is complete, the solution is contacted to the oppositely charged solid phase material to attract, attach, and separate the capture reagent/analyte complex from the fluid sample. With the appropriate indicator reagent, i.e., a second analyte-specific binding substance which is conjugated to a label capable of producing adetectable signal, both sandwich and competitive assays can be performed. The assay reaction complex can be separated from the solution by contact with the oppositely charged solid phase material, and the presence or amount of analyte is monitored by detecting the label of the indicator reagent.

公开(公告)号：DE3686184T2

公开(公告)日：1993-02-25

申请号：DE3686184

申请日：1986-03-10

Applicant: ABBOTT LAB

Inventor： SCHULTZ STEVEN GEORGE

IPC: G01J3/10 ,  G01J3/02 ,  G01J3/04 ,  G01J3/28 ,  G01N21/07 ,  H01L31/0216 ,  G01J3/36 ,  G01J3/00 ,  G01J3/42 ,  G01N21/31 ,  H01L27/14

Abstract: An improved spectrophotometer, especially suitable for use in centrifugal analysis instrumentation, is disclosed. The spectrophotometer is improved by including therein a detector comprising a photodiode array assembly having a photodiode array, a spectral filter assembly situated substantially parallel thereto and in the path of incident light and means for attenuating stray light which would otherwise impinge on each of the photodiodes of the array.

公开(公告)号：ES2016070A4

公开(公告)日：1990-10-16

申请号：ES89101263

申请日：1989-01-25

Applicant: ABBOTT LAB

Inventor： HILTIBRAN ROBERT G ,  JOU YI-HER ,  KLINE STEVEN J ,  SCHULTZ STEVEN GEORGE ,  STROUPE STEPHEN DENHAM

IPC: G01N33/532 ,  G01N33/538 ,  G01N33/543 ,  G01N33/574