Abstract:
The present invention is directed to improving the performance of assays using a disposable assay device which includes a porous material (20) in liquid communication with a capillary track (18). In particular, the capillary track (18) is used in conjunction with the solid support to direct test sample and assay reagents directly to a defined reaction site (22) on or in the porous material (20). Signal development at the reaction site (22) indicates the assay result. The present invention is also directed to the construction of a disposable assay device (10) which includes a capillary track (18). In particular, the capillary track is formed by printing a fluid insoluble material (25a), in the reverse image of the desired capillary track (18), on a film layer (23) and then capping the printed material. Alternatively, the capillary track (18) is formed by printing a fluid insoluble material, in the reverse image (30) of the desired capillary track (18), on a porous material (25b) which is then sandwiched between two film layers (23, 26). The present invention is also directed to a drop-forming means (50) which may incorporate one or more assay reagents to allow for improved sample processing and reagent addition, mixing and incubation.
Abstract:
Disclosed is an improved device for performing agglutination reactions having at least one chamber (25) having a proximate zone (6) and a distal zone (7), in which chamber (25) agglutination reactions can be performed. The improvement comprises utilization in the device of means for controlling overall rate of liquid flow through the chamber (25), the means comprising a layer (2) of liquid absorbent material selectively impregnated with a substance to form an impregnated region (26) and a non-impregnated region (4) wherein the non-impregnated region (4) is liquid absorbent and the impregnated region (26) is liquid-occlusive. The non-impregnated region (4) is in liquid communication with the distal zone (7) of the chamber (25).
Abstract:
The present invention is directed to improving the performance of assays using a disposable assay device which includes a porous material in liquid communication with a capillary track. In particular, the capillary track is used in conjunction with the solid support to direct test sample and assay reagents directly to a defined reaction site on or in the porous material. Signal development at the reaction site indicates the assay result. The present invention is also directed to the construction of a disposable assay device which includes a capillary track. In particular, the cappillary track is formed by printing a fluid insoluble material, in the reverse image of the desired capillary track, on a film layer and then capping the printed material. Alternatively, the capillary track is formed by printing a fluid insoluble material, in the reverse image of the desired capillary track, on a porous material which is then sandwiched between two film layers.
Abstract:
Disclosed is an improved device (10) for performing agglutination reactions having at least one chamber (10) having a reaction zone and a distal zone, in which chamber agglutination reactions can be performed. The improvement comprises utilization in the device of means for controlling the overall rate of liquid flow through the chamber (22), the means comprising a layer of liquid absorbant material (22) selectively impregnated with a substance to form an impregnated region and a non-impregnated region wherein the non-impregnated region is liquid absorbant and the impregnated region is liquid-occlusive. The non-impregnated region is in liquid communication with the distal zone of the chamber (10).
Abstract:
Disclosed is a device for performing agglutination reactions comprising: in adherent relationship, a first wettable layer (1), a second liquid-occlusive layer (2) parallel to and overlying the first layer (1), and a third layer (3) parallel to and overlying the second layer (2) and having a window for observing particles. The second layer is interposed between, and is in adherent relationship to the first and third layers. The second layer (2) has at least one general slot (25) defining a channel for directing liquid conducted by capillary action through a chamber defined by the slot in conjunction with the first (1) and third (3) layers. The chamber has a proximate zone and a distal zone. The geometry of the chamber is modified to provide flow paths having different rates of flow per unit area along the paths. This arrangement of such different paths in the chamber allows agglutination reactions in the chamber to result in the formation of a non-random pattern of aggregated particles in the distal zone of the chamber. Such a non-random pattern is more readily observable than a random pattern of aggregated particles.
Abstract:
Dispositif amélioré (10) permettant de procéder à des réactions d'agglutination, comportant au moins une chambre (10) présentant une zone de réaction et une zone distale, les réactions d'agglutination ayant lieu dans ladite chambre. L'amélioration comprend l'utilisation dans le dispositif d'un moyen de régulation du débit global de liquide dans la chambre (22), le moyen comprenant une couche de matière absorbant le liquide (22) sélectivement imprégnée d'une substance afin de former une région imprégnée ainsi qu'une région non imprégnée, ladite région non imprégnée absorbant le liquide et ladite région imprégnée arrêtant le liquide. Ladite région non imprégnée est en communication liquide avec la zone distale de la chambre (10).
Abstract:
Dispositif amélioré permettant de procéder à des réactions d'agglutination et comportant au moins une chambre (25) présentant une zone proximale (6) et une zone distale (7), les réactions d'agglutination intervenant dans ladite chambre (25). L'amélioration comprend l'utilisation dans le dispositif d'un moyen de régulation de la vitesse globale de l'écoulement de liquide à travers ladite chambre (25), le moyen comprenant une couche (2) de matière absorbant du liquide sélectivement imprégnée d'une substance afin de former une région imprégnée (26) ainsi qu'une région non imprégnée (4), ladite région non imprégnée (4) absorbant le liquide et ladite région imprégnée (26) arrêtant le liquide. Ladite région non imprégnée (4) est en communication fluide avec la zone distale (7) de la chambre (25).
Abstract:
The present invention is directed to improving the performance of assays using a disposable assay device which includes a porous material (20) in liquid communication with a capillary track (18). In particular, the capillary track (18) is used in conjunction with the solid support to direct test sample and assay reagents directly to a defined reaction site (22) on or in the porous material (20). Signal development at the reaction site (22) indicates the assay result. The present invention is also directed to the construction of a disposable assay device (10) which includes a capillary track (18). In particular, the capillary track is formed by printing a fluid insoluble material (25a), in the reverse image of the desired capillary track (18), on a film layer (23) and then capping the printed material. Alternatively, the capillary track (18) is formed by printing a fluid insoluble material, in the reverse image (30) of the desired capillary track (18), on a porous material (25b) which is then sandwiched between two film layers (23, 26). The present invention is also directed to a drop-forming means (50) which may incorporate one or more assay reagents to allow for improved sample processing and reagent addition, mixing and incubation.
Abstract:
The present invention is directed to improving the performance of assays using a disposable assay device which includes a porous material in liquid communication with a capillary track. In particular, the capillary track is used in conjunction with the solid support to direct test sample and assay reagents directly to a defined reaction site on or in the porous material. Signal development at the reaction site indicates the assay result. The present invention is also directed to the construction of a disposable assay device which includes a capillary track. In particular, the cappillary track is formed by printing a fluid insoluble material, in the reverse image of the desired capillary track, on a film layer and then capping the printed material. Alternatively, the capillary track is formed by printing a fluid insoluble material, in the reverse image of the desired capillary track, on a porous material which is then sandwiched between two film layers.
Abstract:
Dispositif permettant d'obtenir des réactions d'agglutination et comprenant: dans une relation d'adhérence, une première couche mouillable (1), une seconde arrêtant le liquide (2) parallèle à la première couche (1) qu'elle recouvre, ainsi qu'une troisième couche (3) parallèle à la seconde couche (2) qu'elle recouvre et dotée d'une fenêtre permettant l'observation de particules. La seconde couche est interposée entre les première et troisième couches avec lesquelles elle est en relation d'adhérence. La seconde couche (2) présente au moins une fente générale (25) définissant un canal permettant d'orienter du liquide conduit par capillarité à travers une chambre définie par la fente conjointement avec les première (1) et troisième (3) couches. La chambre présente une zone proximale ainsi qu'une zone distale. La géométrie de ladite chambre est modifiée afin de ménager des chemins d'écoulement ayant différents débits par zone unitaire le long desdits chemins. Cet agencement desdits chemins différents permet d'obtenir des réactions d'agglutination dans ladite chambre avec comme résultat la formation d'une configuration non aléatoire de particules agrégées dans la zone distale de ladite chambre. Ladite configuration non aléatoire est plus facilement observable qu'une configuration aléatoire de particules agrégées.