公开(公告)号：WO2017004463A1

公开(公告)日：2017-01-05

申请号：PCT/US2016/040579

申请日：2016-06-30

Applicant: ABBOTT LABORATORIES

Inventor： HUFF, Jeffrey B. ,  HAYDEN, Mark A. ,  KARABATSOS, Peter J. ,  SCHAPALS, Andrew S. ,  MUERHOFF, Anthony S. ,  BOGDAN, Maria Felicia ,  LEARY, Thomas ,  HOLETS-MCCORMACK, Shelley R. ,  LAURENSON, Sophie ,  FISCHER, Andrew T. ,  HAACK, Richard ,  HERSHBERGER, Stefan ,  HOUSE, Dustin ,  QIAO, Lei ,  MURPHY, M. Shawn ,  POPE, Mark R. ,  PRIETO-BALLENGEE, Edna M. ,  RUAN, QiaoQiao ,  SONI, Pathik ,  TETIN, Sergey ,  YARNELL, Lyle

IPC: G01N33/53 ,  G01N33/487 ,  G01N33/543

CPC classification number: G01N33/54306 ,  G01N33/48721 ,  G01N33/5308 ,  G01N2458/10

Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. Other aspects of the methods use a phage conjugate as the detection moiety. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.

Abstract translation: 公开了使用纳米孔分析物分析的方法，装置和系统。 方法，装置和系统利用第一和第二结合成员，每个结合成员都特异性结合生物样品中的分析物。 该方法还包括检测和/或计数连接到第二结合成员的可切割标签，并将标签的存在和/或数量与分析物的存在和/或浓度相关联。 方法的某些方面不涉及标签，而是可以直接检测/量化第二结合成员。 方法的其他方面使用噬菌体缀合物作为检测部分。 检测和/或计数可以通过将标签/第二结合部件移位通过纳米孔来进行。 还提供了被编程以执行所公开的方法的设备和系统。

公开(公告)号：WO2019213583A1

公开(公告)日：2019-11-07

申请号：PCT/US2019/030679

申请日：2019-05-03

Applicant: ABBOTT LABORATORIES

Inventor： TETIN, Sergey ,  HUFF, Jeffrey B. ,  SKINNER, Joseph P. ,  MACDONALD, Patrick ,  RUAN, Qiaoqiao

IPC: G01N33/53 ,  G01N33/543

Abstract: The disclosure provides a method for an enhanced detection of an analyte present in a biological sample. After the formation of the analyte/specific binding member(s)/detectable label complex, the labels are eluted and a first aliquot of eluant is brought into contact with a solid support, wherein the solid support comprises immobilized thereto specific binding member that specifically binds to the label, removing the first aliquot from the solid support and contacting the solid support with a second aliquot of the eluted label, and repeating the above steps, such that the label is concentrated on the solid support for further analysis to quantify the analyte in the biological sample.

公开(公告)号：WO2016161402A1

公开(公告)日：2016-10-06

申请号：PCT/US2016/025787

申请日：2016-04-02

Applicant: ABBOTT LABORATORIES

Inventor： HUFF, Jeffrey B. ,  HAYDEN, Mark A. ,  KARABATSOS, Peter J. ,  SCHAPALS, Andrew S. ,  MUERHOFF, Anthony S. ,  BOGDAN, Felicia ,  LEARY, Thomas ,  HOLETS-MCCORMACK, Shelley R. ,  LAURENSON, Sophie ,  FISCHER, Andrew T. ,  HAACK, Richard ,  HERSHBERGER, Stefan ,  HOUSE, Dustin ,  QIAO, Lei ,  MURPHY, M. Shawn ,  POPE, Mark R. ,  PRIETO-BALLENGEE, Edna M. ,  RUAN, QiaoQiao ,  SONI, Pathik ,  TETIN, Sergey ,  YARNELL, Lyle

IPC: G01N33/53 ,  G01N33/487 ,  G01N33/543

CPC classification number: G01N33/48721 ,  B01L3/502761 ,  B01L2300/0645 ,  B01L2400/0406 ,  G01N33/5308 ,  G01N33/54306 ,  G01N2458/10

Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.

Abstract translation: 公开了使用纳米孔分析物分析的方法，装置和系统。 方法，装置和系统利用第一和第二结合成员，每个结合成员都特异性结合生物样品中的分析物。 该方法还包括检测和/或计数连接到第二结合成员的可切割标签，并将标签的存在和/或数量与分析物的存在和/或浓度相关联。 方法的某些方面不涉及标签，而是可以直接检测/量化第二结合成员。 检测和/或计数可以通过将标签/第二结合部件移位通过纳米孔来进行。 还提供了被编程以执行所公开的方法的设备和系统。

公开(公告)号：EP3788371A1

公开(公告)日：2021-03-10

申请号：EP19727177.8

申请日：2019-05-03

Applicant: Abbott Laboratories

Inventor： TETIN, Sergey ,  HUFF, Jeffrey B. ,  SKINNER, Joseph P. ,  MACDONALD, Patrick ,  RUAN, Qiaoqiao

IPC: G01N33/53 ,  G01N33/543

公开(公告)号：EP3839507A1

公开(公告)日：2021-06-23

申请号：EP21156369.7

申请日：2016-04-02

Applicant: Abbott Laboratories

Inventor： HUFF, Jeffrey B ,  HAYDEN, Mark A ,  KARABATSOS, Peter J ,  SCHAPALS, Andrew S ,  MUERHOFF, Anthony S ,  BOGDAN, Felicia ,  LEARY, Thomas ,  HOLETS-MCCORMACK, Shelley R ,  LAURENSON, Sophie ,  FISCHER, Andrew T ,  HAACK, Richard ,  HERSHBERGER, Stefan ,  HOUSE, Dustin ,  QIAO, Lei ,  MURPHY, M. Shawn ,  POPE, Mark R. ,  PRIETO-BALLENGEE, Edna M. ,  RUAN, QiaoQiao ,  SONI, Pathik ,  TETIN, Sergey ,  YARNELL, Lyle

IPC: G01N33/53 ,  G01N33/487 ,  G01N33/543

Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.

公开(公告)号：EP3278108A1

公开(公告)日：2018-02-07

申请号：EP16717521.5

申请日：2016-04-02

Applicant: Abbott Laboratories

Inventor： HUFF, Jeffrey B. ,  HAYDEN, Mark A. ,  KARABATSOS, Peter J. ,  SCHAPALS, Andrew S. ,  MUERHOFF, Anthony S. ,  BOGDAN, Felicia ,  LEARY, Thomas ,  HOLETS-MCCORMACK, Shelley R. ,  LAURENSON, Sophie ,  FISCHER, Andrew T. ,  HAACK, Richard ,  HERSHBERGER, Stefan ,  HOUSE, Dustin ,  QIAO, Lei ,  MURPHY, M. Shawn ,  POPE, Mark R. ,  PRIETO-BALLENGEE, Edna M. ,  RUAN, QiaoQiao ,  SONI, Pathik ,  TETIN, Sergey ,  YARNELL, Lyle

IPC: G01N33/53 ,  G01N33/487 ,  G01N33/543

CPC classification number: G01N33/48721 ,  B01L3/502761 ,  B01L2300/0645 ,  B01L2400/0406 ,  G01N33/5308 ,  G01N33/54306 ,  G01N2458/10

Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.