公开(公告)号：WO2020140071A1

公开(公告)日：2020-07-02

申请号：PCT/US2019/068801

申请日：2019-12-27

Applicant: ABBOTT LABORATORIES

Inventor： RUAN, Qiaoqiao ,  MACDONALD, Patrick J. ,  SWIFT, Kerry M. ,  TETIN, Sergey Y. ,  CALFIN, Brenda ,  LIN, Zhen ,  HAACK, Richard ,  POPE, Mark R. ,  PROSTKO, John ,  QIU, Xiaoxing ,  BOGDAN, Felicia

IPC: G01N33/543 ,  G01N33/566 ,  G01N33/58

Abstract: The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a cross-linked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provided are methods of analyzing two or more analytes of interest in a biological sample in a single assay using microparticles and detection conjugates comprising different fluorophore labels, acquiring transmitted light and fluorescent images of the microparticles, and using a customized image analysis process to analyze the acquired images.

公开(公告)号：WO2016161402A1

公开(公告)日：2016-10-06

申请号：PCT/US2016/025787

申请日：2016-04-02

Applicant: ABBOTT LABORATORIES

Inventor： HUFF, Jeffrey B. ,  HAYDEN, Mark A. ,  KARABATSOS, Peter J. ,  SCHAPALS, Andrew S. ,  MUERHOFF, Anthony S. ,  BOGDAN, Felicia ,  LEARY, Thomas ,  HOLETS-MCCORMACK, Shelley R. ,  LAURENSON, Sophie ,  FISCHER, Andrew T. ,  HAACK, Richard ,  HERSHBERGER, Stefan ,  HOUSE, Dustin ,  QIAO, Lei ,  MURPHY, M. Shawn ,  POPE, Mark R. ,  PRIETO-BALLENGEE, Edna M. ,  RUAN, QiaoQiao ,  SONI, Pathik ,  TETIN, Sergey ,  YARNELL, Lyle

IPC: G01N33/53 ,  G01N33/487 ,  G01N33/543

CPC classification number: G01N33/48721 ,  B01L3/502761 ,  B01L2300/0645 ,  B01L2400/0406 ,  G01N33/5308 ,  G01N33/54306 ,  G01N2458/10

Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.

Abstract translation: 公开了使用纳米孔分析物分析的方法，装置和系统。 方法，装置和系统利用第一和第二结合成员，每个结合成员都特异性结合生物样品中的分析物。 该方法还包括检测和/或计数连接到第二结合成员的可切割标签，并将标签的存在和/或数量与分析物的存在和/或浓度相关联。 方法的某些方面不涉及标签，而是可以直接检测/量化第二结合成员。 检测和/或计数可以通过将标签/第二结合部件移位通过纳米孔来进行。 还提供了被编程以执行所公开的方法的设备和系统。

公开(公告)号：WO2017004463A1

公开(公告)日：2017-01-05

申请号：PCT/US2016/040579

申请日：2016-06-30

Applicant: ABBOTT LABORATORIES

Inventor： HUFF, Jeffrey B. ,  HAYDEN, Mark A. ,  KARABATSOS, Peter J. ,  SCHAPALS, Andrew S. ,  MUERHOFF, Anthony S. ,  BOGDAN, Maria Felicia ,  LEARY, Thomas ,  HOLETS-MCCORMACK, Shelley R. ,  LAURENSON, Sophie ,  FISCHER, Andrew T. ,  HAACK, Richard ,  HERSHBERGER, Stefan ,  HOUSE, Dustin ,  QIAO, Lei ,  MURPHY, M. Shawn ,  POPE, Mark R. ,  PRIETO-BALLENGEE, Edna M. ,  RUAN, QiaoQiao ,  SONI, Pathik ,  TETIN, Sergey ,  YARNELL, Lyle

IPC: G01N33/53 ,  G01N33/487 ,  G01N33/543

CPC classification number: G01N33/54306 ,  G01N33/48721 ,  G01N33/5308 ,  G01N2458/10

Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. Other aspects of the methods use a phage conjugate as the detection moiety. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.

Abstract translation: 公开了使用纳米孔分析物分析的方法，装置和系统。 方法，装置和系统利用第一和第二结合成员，每个结合成员都特异性结合生物样品中的分析物。 该方法还包括检测和/或计数连接到第二结合成员的可切割标签，并将标签的存在和/或数量与分析物的存在和/或浓度相关联。 方法的某些方面不涉及标签，而是可以直接检测/量化第二结合成员。 方法的其他方面使用噬菌体缀合物作为检测部分。 检测和/或计数可以通过将标签/第二结合部件移位通过纳米孔来进行。 还提供了被编程以执行所公开的方法的设备和系统。

公开(公告)号：EP4375669A3

公开(公告)日：2024-07-24

申请号：EP24167359.9

申请日：2019-12-27

Applicant: Abbott Laboratories

Inventor： RUAN, Qiaoqiao ,  MACDONALD, Patrick J. ,  SWIFT, Kerry M. ,  TETIN, Sergey Y. ,  CALFIN, Brenda ,  LIN, Zhen ,  HAACK, Richard ,  POPE, Mark R. ,  PROSTKO, John ,  QIU, Xiaoxing ,  BOGDAN, M. Felicia

IPC: G01N33/543 ,  G01N33/566 ,  G01N33/58

CPC classification number: G01N33/543 ,  G01N33/566 ,  G01N33/582

Abstract: The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a crosslinked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provided are methods of analyzing two or more analytes of interest in a biological sample in a single assay using microparticles and detection conjugates comprising different fluorophore labels, acquiring transmitted light and fluorescent images of the microparticles, and using a customized image analysis process to analyze the acquired images.

公开(公告)号：EP3903103A1

公开(公告)日：2021-11-03

申请号：EP19839791.1

申请日：2019-12-27

Applicant: Abbott Laboratories

Inventor： RUAN, Qiaoqiao ,  MACDONALD, Patrick J. ,  SWIFT, Kerry M. ,  TETIN, Sergey Y. ,  CALFIN, Brenda ,  LIN, Zhen ,  HAACK, Richard ,  POPE, Mark R. ,  PROSTKO, John ,  QIU, Xiaoxing ,  BOGDAN, M. Felicia

IPC: G01N33/543 ,  G01N33/566 ,  G01N33/58

公开(公告)号：EP4375669A2

公开(公告)日：2024-05-29

申请号：EP24167359.9

申请日：2019-12-27

Applicant: Abbott Laboratories

Inventor： RUAN, Qiaoqiao ,  MACDONALD, Patrick J. ,  SWIFT, Kerry M. ,  TETIN, Sergey Y. ,  CALFIN, Brenda ,  LIN, Zhen ,  HAACK, Richard ,  POPE, Mark R. ,  PROSTKO, John ,  QIU, Xiaoxing ,  BOGDAN, M. Felicia

IPC: G01N33/58

CPC classification number: G01N33/543 ,  G01N33/566 ,  G01N33/582

Abstract: The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a crosslinked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provided are methods of analyzing two or more analytes of interest in a biological sample in a single assay using microparticles and detection conjugates comprising different fluorophore labels, acquiring transmitted light and fluorescent images of the microparticles, and using a customized image analysis process to analyze the acquired images.

公开(公告)号：EP3839507A1

公开(公告)日：2021-06-23

申请号：EP21156369.7

申请日：2016-04-02

Applicant: Abbott Laboratories

Inventor： HUFF, Jeffrey B ,  HAYDEN, Mark A ,  KARABATSOS, Peter J ,  SCHAPALS, Andrew S ,  MUERHOFF, Anthony S ,  BOGDAN, Felicia ,  LEARY, Thomas ,  HOLETS-MCCORMACK, Shelley R ,  LAURENSON, Sophie ,  FISCHER, Andrew T ,  HAACK, Richard ,  HERSHBERGER, Stefan ,  HOUSE, Dustin ,  QIAO, Lei ,  MURPHY, M. Shawn ,  POPE, Mark R. ,  PRIETO-BALLENGEE, Edna M. ,  RUAN, QiaoQiao ,  SONI, Pathik ,  TETIN, Sergey ,  YARNELL, Lyle

IPC: G01N33/53 ,  G01N33/487 ,  G01N33/543

Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.

公开(公告)号：EP3278108A1

公开(公告)日：2018-02-07

申请号：EP16717521.5

申请日：2016-04-02

Applicant: Abbott Laboratories

Inventor： HUFF, Jeffrey B. ,  HAYDEN, Mark A. ,  KARABATSOS, Peter J. ,  SCHAPALS, Andrew S. ,  MUERHOFF, Anthony S. ,  BOGDAN, Felicia ,  LEARY, Thomas ,  HOLETS-MCCORMACK, Shelley R. ,  LAURENSON, Sophie ,  FISCHER, Andrew T. ,  HAACK, Richard ,  HERSHBERGER, Stefan ,  HOUSE, Dustin ,  QIAO, Lei ,  MURPHY, M. Shawn ,  POPE, Mark R. ,  PRIETO-BALLENGEE, Edna M. ,  RUAN, QiaoQiao ,  SONI, Pathik ,  TETIN, Sergey ,  YARNELL, Lyle

IPC: G01N33/53 ,  G01N33/487 ,  G01N33/543

CPC classification number: G01N33/48721 ,  B01L3/502761 ,  B01L2300/0645 ,  B01L2400/0406 ,  G01N33/5308 ,  G01N33/54306 ,  G01N2458/10

Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.