公开(公告)号：WO2020140071A1

公开(公告)日：2020-07-02

申请号：PCT/US2019/068801

申请日：2019-12-27

Applicant: ABBOTT LABORATORIES

Inventor： RUAN, Qiaoqiao ,  MACDONALD, Patrick J. ,  SWIFT, Kerry M. ,  TETIN, Sergey Y. ,  CALFIN, Brenda ,  LIN, Zhen ,  HAACK, Richard ,  POPE, Mark R. ,  PROSTKO, John ,  QIU, Xiaoxing ,  BOGDAN, Felicia

IPC: G01N33/543 ,  G01N33/566 ,  G01N33/58

Abstract: The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a cross-linked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provided are methods of analyzing two or more analytes of interest in a biological sample in a single assay using microparticles and detection conjugates comprising different fluorophore labels, acquiring transmitted light and fluorescent images of the microparticles, and using a customized image analysis process to analyze the acquired images.

公开(公告)号：WO2017004463A1

公开(公告)日：2017-01-05

申请号：PCT/US2016/040579

申请日：2016-06-30

Applicant: ABBOTT LABORATORIES

Inventor： HUFF, Jeffrey B. ,  HAYDEN, Mark A. ,  KARABATSOS, Peter J. ,  SCHAPALS, Andrew S. ,  MUERHOFF, Anthony S. ,  BOGDAN, Maria Felicia ,  LEARY, Thomas ,  HOLETS-MCCORMACK, Shelley R. ,  LAURENSON, Sophie ,  FISCHER, Andrew T. ,  HAACK, Richard ,  HERSHBERGER, Stefan ,  HOUSE, Dustin ,  QIAO, Lei ,  MURPHY, M. Shawn ,  POPE, Mark R. ,  PRIETO-BALLENGEE, Edna M. ,  RUAN, QiaoQiao ,  SONI, Pathik ,  TETIN, Sergey ,  YARNELL, Lyle

IPC: G01N33/53 ,  G01N33/487 ,  G01N33/543

CPC classification number: G01N33/54306 ,  G01N33/48721 ,  G01N33/5308 ,  G01N2458/10

Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. Other aspects of the methods use a phage conjugate as the detection moiety. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.

Abstract translation: 公开了使用纳米孔分析物分析的方法，装置和系统。 方法，装置和系统利用第一和第二结合成员，每个结合成员都特异性结合生物样品中的分析物。 该方法还包括检测和/或计数连接到第二结合成员的可切割标签，并将标签的存在和/或数量与分析物的存在和/或浓度相关联。 方法的某些方面不涉及标签，而是可以直接检测/量化第二结合成员。 方法的其他方面使用噬菌体缀合物作为检测部分。 检测和/或计数可以通过将标签/第二结合部件移位通过纳米孔来进行。 还提供了被编程以执行所公开的方法的设备和系统。

公开(公告)号：WO2021026403A1

公开(公告)日：2021-02-11

申请号：PCT/US2020/045297

申请日：2020-08-06

Applicant: ABBOTT LABORATORIES

Inventor： POPE, Mark R. ,  HERSHBERGER, Stefan J. ,  RUAN, Qiaoqiao ,  SWIFT, Kerry M. ,  TETIN, Sergey Y. ,  MACDONALD, Patrick ,  HAACK, Richard A.

IPC: G01N33/58

Abstract: The disclosure provides kits and methods for detecting a substance that interferes with detection of an analyte in a sample and for expanding the dynamic range and reducing the hook effect of an immunoassay. The kits and methods employ two conjugates with two different detectable labels, at least one of which is a chemiluminescent compound of Formula (I).

公开(公告)号：WO2016161402A1

公开(公告)日：2016-10-06

申请号：PCT/US2016/025787

申请日：2016-04-02

Applicant: ABBOTT LABORATORIES

Inventor： HUFF, Jeffrey B. ,  HAYDEN, Mark A. ,  KARABATSOS, Peter J. ,  SCHAPALS, Andrew S. ,  MUERHOFF, Anthony S. ,  BOGDAN, Felicia ,  LEARY, Thomas ,  HOLETS-MCCORMACK, Shelley R. ,  LAURENSON, Sophie ,  FISCHER, Andrew T. ,  HAACK, Richard ,  HERSHBERGER, Stefan ,  HOUSE, Dustin ,  QIAO, Lei ,  MURPHY, M. Shawn ,  POPE, Mark R. ,  PRIETO-BALLENGEE, Edna M. ,  RUAN, QiaoQiao ,  SONI, Pathik ,  TETIN, Sergey ,  YARNELL, Lyle

IPC: G01N33/53 ,  G01N33/487 ,  G01N33/543

CPC classification number: G01N33/48721 ,  B01L3/502761 ,  B01L2300/0645 ,  B01L2400/0406 ,  G01N33/5308 ,  G01N33/54306 ,  G01N2458/10

Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.

Abstract translation: 公开了使用纳米孔分析物分析的方法，装置和系统。 方法，装置和系统利用第一和第二结合成员，每个结合成员都特异性结合生物样品中的分析物。 该方法还包括检测和/或计数连接到第二结合成员的可切割标签，并将标签的存在和/或数量与分析物的存在和/或浓度相关联。 方法的某些方面不涉及标签，而是可以直接检测/量化第二结合成员。 检测和/或计数可以通过将标签/第二结合部件移位通过纳米孔来进行。 还提供了被编程以执行所公开的方法的设备和系统。

公开(公告)号：EP4375669A2

公开(公告)日：2024-05-29

申请号：EP24167359.9

申请日：2019-12-27

Applicant: Abbott Laboratories

Inventor： RUAN, Qiaoqiao ,  MACDONALD, Patrick J. ,  SWIFT, Kerry M. ,  TETIN, Sergey Y. ,  CALFIN, Brenda ,  LIN, Zhen ,  HAACK, Richard ,  POPE, Mark R. ,  PROSTKO, John ,  QIU, Xiaoxing ,  BOGDAN, M. Felicia

IPC: G01N33/58

CPC classification number: G01N33/543 ,  G01N33/566 ,  G01N33/582

Abstract: The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a crosslinked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provided are methods of analyzing two or more analytes of interest in a biological sample in a single assay using microparticles and detection conjugates comprising different fluorophore labels, acquiring transmitted light and fluorescent images of the microparticles, and using a customized image analysis process to analyze the acquired images.

公开(公告)号：EP3839507A1

公开(公告)日：2021-06-23

申请号：EP21156369.7

申请日：2016-04-02

Applicant: Abbott Laboratories

Inventor： HUFF, Jeffrey B ,  HAYDEN, Mark A ,  KARABATSOS, Peter J ,  SCHAPALS, Andrew S ,  MUERHOFF, Anthony S ,  BOGDAN, Felicia ,  LEARY, Thomas ,  HOLETS-MCCORMACK, Shelley R ,  LAURENSON, Sophie ,  FISCHER, Andrew T ,  HAACK, Richard ,  HERSHBERGER, Stefan ,  HOUSE, Dustin ,  QIAO, Lei ,  MURPHY, M. Shawn ,  POPE, Mark R. ,  PRIETO-BALLENGEE, Edna M. ,  RUAN, QiaoQiao ,  SONI, Pathik ,  TETIN, Sergey ,  YARNELL, Lyle

IPC: G01N33/53 ,  G01N33/487 ,  G01N33/543

Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.

公开(公告)号：EP3278108A1

公开(公告)日：2018-02-07

申请号：EP16717521.5

申请日：2016-04-02

Applicant: Abbott Laboratories

Inventor： HUFF, Jeffrey B. ,  HAYDEN, Mark A. ,  KARABATSOS, Peter J. ,  SCHAPALS, Andrew S. ,  MUERHOFF, Anthony S. ,  BOGDAN, Felicia ,  LEARY, Thomas ,  HOLETS-MCCORMACK, Shelley R. ,  LAURENSON, Sophie ,  FISCHER, Andrew T. ,  HAACK, Richard ,  HERSHBERGER, Stefan ,  HOUSE, Dustin ,  QIAO, Lei ,  MURPHY, M. Shawn ,  POPE, Mark R. ,  PRIETO-BALLENGEE, Edna M. ,  RUAN, QiaoQiao ,  SONI, Pathik ,  TETIN, Sergey ,  YARNELL, Lyle

IPC: G01N33/53 ,  G01N33/487 ,  G01N33/543

CPC classification number: G01N33/48721 ,  B01L3/502761 ,  B01L2300/0645 ,  B01L2400/0406 ,  G01N33/5308 ,  G01N33/54306 ,  G01N2458/10

Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.

公开(公告)号：EP4375669A3

公开(公告)日：2024-07-24

申请号：EP24167359.9

申请日：2019-12-27

Applicant: Abbott Laboratories

Inventor： RUAN, Qiaoqiao ,  MACDONALD, Patrick J. ,  SWIFT, Kerry M. ,  TETIN, Sergey Y. ,  CALFIN, Brenda ,  LIN, Zhen ,  HAACK, Richard ,  POPE, Mark R. ,  PROSTKO, John ,  QIU, Xiaoxing ,  BOGDAN, M. Felicia

IPC: G01N33/543 ,  G01N33/566 ,  G01N33/58

CPC classification number: G01N33/543 ,  G01N33/566 ,  G01N33/582

Abstract: The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a crosslinked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provided are methods of analyzing two or more analytes of interest in a biological sample in a single assay using microparticles and detection conjugates comprising different fluorophore labels, acquiring transmitted light and fluorescent images of the microparticles, and using a customized image analysis process to analyze the acquired images.

公开(公告)号：EP4010707A1

公开(公告)日：2022-06-15

申请号：EP20768731.0

申请日：2020-08-06

Applicant: Abbott Laboratories

Inventor： POPE, Mark R. ,  HERSHBERGER, Stefan J. ,  RUAN, Qiaoqiao ,  SWIFT, Kerry M. ,  TETIN, Sergey Y. ,  MACDONALD, Patrick ,  HAACK, Richard A.

IPC: G01N33/58

公开(公告)号：EP3903103A1

公开(公告)日：2021-11-03

申请号：EP19839791.1

申请日：2019-12-27

Applicant: Abbott Laboratories

Inventor： RUAN, Qiaoqiao ,  MACDONALD, Patrick J. ,  SWIFT, Kerry M. ,  TETIN, Sergey Y. ,  CALFIN, Brenda ,  LIN, Zhen ,  HAACK, Richard ,  POPE, Mark R. ,  PROSTKO, John ,  QIU, Xiaoxing ,  BOGDAN, M. Felicia

IPC: G01N33/543 ,  G01N33/566 ,  G01N33/58