公开(公告)号：JPH02207792A

公开(公告)日：1990-08-17

申请号：JP2707989

申请日：1989-02-06

Applicant: AGENCY IND SCIENCE TECHN

Inventor： NAKAHARA HARUO ,  SUZUKI OSAMU ,  YOKOCHI TOSHIHIRO ,  YAMAOKA MASAKAZU ,  KAMISAKA YASUSHI

IPC: C12P7/64 ,  C12N11/02

Abstract: PURPOSE:To efficiently produce 13-hydroperoxy-gamma-linolenic acid by treating gamma-linolenic acid with soybean lipoxygenase in the presence of a specific buffer solution and a surfactant. CONSTITUTION:gamma-Linolenic acid is treated with soybean lipoxygenase in a two- phase system of hexane-boric acid buffer solution adjusted to pH >=9 in the presence of a surfactant. Consequently, gamma-linolenic acid is converted into 13- hydroxyperoxy-gamma-linolenic acid. Second oxygen addition of prepared 13- hydroxyperoxy-gamma-linolenic acid is prevented by immobilizing soybean lipoxygenase to chitosan and highly concentrated 13-hydroxyperoxy-gamma-linolenic acid is obtained. Immobilization of soybean lipoxygenase to chitosan is carried out through glutaraldehyde.

公开(公告)号：JPH06113868A

公开(公告)日：1994-04-26

申请号：JP28551892

申请日：1992-09-30

Applicant: AGENCY IND SCIENCE TECHN

Inventor： YAGI HISAAKI ,  YOKOCHI TOSHIHIRO ,  KAMISAKA YASUSHI ,  YAMAOKA MASAKAZU ,  NAKAHARA HARUROU

IPC: C12P7/40 ,  C12R1/38

Abstract: PURPOSE:To efficiently obtain a specific cyclopropanefatty acid using Pseudomonas bacteria. CONSTITUTION:Pseudomonas bacteria are cultured in a medium containing propanol and/or propionic acid or a salt thereof to obtain the objective fatty acid, i.e., 2-pentyl-1-cyclopropane-1-decanoic acid or 2-heptyl-1-cyclopropane-1- octanoic acid.

公开(公告)号：JPH05302087A

公开(公告)日：1993-11-16

申请号：JP13204492

申请日：1992-04-24

Applicant: AGENCY IND SCIENCE TECHN

Inventor： YOKOYAMA SHINYA ,  TSUCHIDE YUTAKA ,  SAWAYAMA SHIGEKI ,  MINOWA TOMOAKI ,  YOKOCHI TOSHIHIRO ,  KAMISAKA YASUSHI ,  NAKAHARA HARUROU ,  SUZUKI OSAMU

IPC: C10G1/00 ,  C10L1/00 ,  C12P1/00

Abstract: PURPOSE:To provide a method by which a cell which accumulates oil or fat is liquefied simply and effectively to give a substance like heavy oil and simultaneously bring the liquefaction product into a state with good phase separation properties. CONSTITUTION:A cell which accumulates oil or fat is kept at high temp. and pressure in the pressence of an aq. medium to thereby be liquefied to give a substance like heavy oil. The conditions of the liquefaction are controlled so that the liquefaction product contg. the substance is brought into a state with good phase separation properties.

公开(公告)号：JPS6398392A

公开(公告)日：1988-04-28

申请号：JP24319686

申请日：1986-10-15

Applicant: AGENCY IND SCIENCE TECHN ,  MEIJI SEIKA KAISHA

Inventor： NAKAHARA HARUROU ,  YOKOCHI TOSHIHIRO ,  KAMISAKA YASUSHI ,  SUZUKI OSAMU ,  HIRUTA OSAMU

IPC: C12P7/40 ,  C12P7/64

Abstract: PURPOSE:To exclusively produce the titled substance in high conversion, by treating gamma-linolenic acid with soybean lipoxygenase in a system of an organic solvent and an aqueous solution in the presence of an anionic surfactant. CONSTITUTION:gamma-Linolenic acid is dissolved in a binary phase system composed of an organic solvent (e.g., hexane, heptane, etc.) and an aqueous solution. The aqueous solution is a boric acid buffer solution of 6.5pH. gamma-Linolenic acid is treated with soybean lipoxygenase in the above binary phase system in the presence of an anionic surfactant such as sodium polyoxyethylene alkyl ether acetate, sarcosinate L-N, etc., to convert the gamma-linolenic acid to 9-hydroperoxy-gamma- linolenic acid. The objective product is recovered by adding hydrochloric acid to the reaction mixture, extracting the oil-soluble fraction with ethyl acetate, subjecting the extract to dehydrochlorination and dehydration and developing by a reversed phase thin-layer chromatography.