公开(公告)号：WO2007067999A3

公开(公告)日：2007-11-29

申请号：PCT/US2006061871

申请日：2006-12-11

Applicant: AMNIS CORP ,  ORTYN WILLIAM ,  BASIJI DAVID ,  FROST KEITH ,  LIANG LUCHUAN ,  BAUER RICHARD ,  HALL BRIAN ,  PERRY DAVID

Inventor： ORTYN WILLIAM ,  BASIJI DAVID ,  FROST KEITH ,  LIANG LUCHUAN ,  BAUER RICHARD ,  HALL BRIAN ,  PERRY DAVID

IPC: G02F1/01

CPC classification number: G02B27/0075 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/6458 ,  G01N2015/1452 ,  G02B21/365 ,  G02B27/0012

Abstract: A high speed, high-resolution flow imaging system is modified to achieve extended depth of field imaging. An optical distortion element is introduced into the flow imaging system. Light from an object, such as a cell, is distorted by the distortion element, such that a point spread function (PSF) of the imaging system is invariant across an extended depth of field. The distorted light is spectrally dispersed, and the dispersed light is used to simultaneously generate a plurality of images. The images are detected, and image processing is used to enhance the detected images by compensating for the distortion, to achieve extended depth of field images of the object. The post image processing preferably involves de-convolution, and requires knowledge of the PSF of the imaging system, as modified by the optical distortion element.

Abstract translation: 改进了高速，高分辨率流动成像系统，以实现扩展的景深成像。 光学失真元件被引入到流动成像系统中。 来自诸如单元的物体的光被失真元件扭曲，使得成像系统的点扩散函数（PSF）在扩展的景深范围内是不变的。 失真光被光谱分散，并且分散的光被用于同时产生多个图像。 检测图像，并且使用图像处理来通过补偿失真来增强检测到的图像，以实现对象的扩展景深图像。 后图像处理优选地涉及去卷积，并且需要由光学失真元件修改的成像系统的PSF的知识。

公开(公告)号：WO0231182A3

公开(公告)日：2002-09-06

申请号：PCT/US0142638

申请日：2001-10-12

Applicant: AMNIS CORP ,  FROST KEITH ,  BASIJI DAVID ,  BAUER RICHARD ,  FINCH ROSALYNDE ,  ORTYN WILLIAM ,  PERRY DAVID

Inventor： FROST KEITH ,  BASIJI DAVID ,  BAUER RICHARD ,  FINCH ROSALYNDE ,  ORTYN WILLIAM ,  PERRY DAVID

IPC: G01N21/64 ,  C07B61/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N15/02 ,  G01N15/10 ,  G01N15/14 ,  G01N21/05 ,  G01N33/48 ,  G01N33/483 ,  G01N33/50 ,  G02B7/28 ,  G02B21/00 ,  G02B27/00 ,  G02B27/14 ,  G01N33/543 ,  C07H21/04 ,  G01J3/28 ,  G01N21/00 ,  G01N33/53

CPC classification number: G02B27/148 ,  B01J2219/00274 ,  C07B2200/11 ,  C12Q1/6816 ,  C12Q1/6869 ,  C12Q2600/156 ,  C40B20/04 ,  C40B60/00 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6456 ,  G01N2015/0294 ,  G01N2015/1018 ,  G01N2015/1472 ,  G01N2015/1479 ,  G01N2021/058 ,  G01N2021/6421 ,  G02B7/28 ,  G02B21/00 ,  G02B27/0012 ,  G02B27/1013 ,  G02B27/126 ,  G02B27/144 ,  G02B27/145 ,  C12Q2565/626 ,  C12Q2563/149 ,  C12Q2563/107

Abstract: Combinatorially-synthesized deoxyribonucleic acid (DNA) oligonucleotides attached to encoded beads that are hybridized to amplified and labeled genomic DNA or ribonucleic acid (RNA) are analyzed using a flow imaging system. Oligonucleotides and corresponding reporters are bound to the surfaces of a plurality of small beads such that different beads bear different oligo sequences. Each bead bears a unique optical signature comprising a predefined number of unique reporters, where each reporter comprises a predefined combination of different fluorochromes. The composite spectral signature in turn identifies the unique nucleotide sequence of its attached oligo chains. This optical signature is rapidly decoded using an imaging system to discriminate the different reporters attached to each bead in a flow in regard to color and spatial position on the bead (see fig. 15).

Abstract translation: 使用流动成像系统分析与编码的珠偶联的与扩增和标记的基因组DNA或核糖核酸（RNA）杂交的组合合成的脱氧核糖核酸（DNA）寡核苷酸。 寡核苷酸和相应的报道结合到多个小珠的表面，使得不同的珠具有不同的寡核苷酸序列。 每个珠具有独特的光学特征，其包含预定数量的唯一记录器，其中每个报告者包括不同荧光染料的预定组合。 复合光谱特征依次识别其连接的寡链的唯一核苷酸序列。 使用成像系统快速解码该光学签名，以鉴别与珠上的颜色和空间位置相关的流中附着于每个珠的不同报告者（参见图15）。

公开(公告)号：EP1330650A4

公开(公告)日：2008-03-19

申请号：EP01979992

申请日：2001-10-12

Applicant: AMNIS CORP

Inventor： FROST KEITH ,  BASIJI DAVID ,  BAUER RICHARD ,  FINCH ROSALYNDE ,  ORTYN WILLIAM ,  PERRY DAVID

IPC: G01N21/64 ,  G01N33/543 ,  B01J19/00 ,  C07B61/00 ,  C07H21/04 ,  C12N15/09 ,  C12Q1/68 ,  G01J3/28 ,  G01N15/02 ,  G01N15/10 ,  G01N15/14 ,  G01N21/05 ,  G01N33/48 ,  G01N33/483 ,  G01N33/50 ,  G01N33/53 ,  G02B7/28 ,  G02B21/00 ,  G02B27/00 ,  G02B27/14

CPC classification number: G02B27/148 ,  B01J2219/00274 ,  C07B2200/11 ,  C12Q1/6816 ,  C12Q1/6869 ,  C12Q2600/156 ,  C40B20/04 ,  C40B60/00 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6456 ,  G01N2015/0294 ,  G01N2015/1018 ,  G01N2015/1472 ,  G01N2015/1479 ,  G01N2021/058 ,  G01N2021/6421 ,  G02B7/28 ,  G02B21/00 ,  G02B27/0012 ,  G02B27/1013 ,  G02B27/126 ,  G02B27/144 ,  G02B27/145 ,  C12Q2565/626 ,  C12Q2563/149 ,  C12Q2563/107

Abstract: Combinatorially-synthesized deoxyribonucleic acid (DNA) oligonucleotides attached to encoded beads that are hybridized to amplified and labeled genomic DNA or ribonucleic acid (RNA) are analyzed using a flow imaging system. Oligonucleotides and corresponding reporters are bound to the surfaces of a plurality of small beads such that different beads bear different oligo sequences. Each bead bears a unique optical signature comprising a predefined number of unique reporters, where each reporter comprises a predefined combination of different fluorochromes. The composite spectral signature in turn identifies the unique nucleotide sequence of its attached oligo chains. This optical signature is rapidly decoded using an imaging system to discriminate the different reporters attached to each bead in a flow in regard to color and spatial position on the bead (see fig. 15).

公开(公告)号：AU1189802A

公开(公告)日：2002-04-22

申请号：AU1189802

申请日：2001-10-12

Applicant: AMNIS CORP

Inventor： FROST KEITH ,  BASIJI DAVID ,  BAUER RICHARD ,  FINCH ROSALYNDE ,  ORTYN WILLIAM ,  PERRY DAVID

IPC: G01N21/64 ,  C07B61/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N15/02 ,  G01N15/10 ,  G01N15/14 ,  G01N21/05 ,  G01N33/48 ,  G01N33/483 ,  G01N33/50 ,  G02B7/28 ,  G02B21/00 ,  G02B27/00 ,  G02B27/14