公开(公告)号：WO0231182A3

公开(公告)日：2002-09-06

申请号：PCT/US0142638

申请日：2001-10-12

Applicant: AMNIS CORP ,  FROST KEITH ,  BASIJI DAVID ,  BAUER RICHARD ,  FINCH ROSALYNDE ,  ORTYN WILLIAM ,  PERRY DAVID

Inventor： FROST KEITH ,  BASIJI DAVID ,  BAUER RICHARD ,  FINCH ROSALYNDE ,  ORTYN WILLIAM ,  PERRY DAVID

IPC: G01N21/64 ,  C07B61/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N15/02 ,  G01N15/10 ,  G01N15/14 ,  G01N21/05 ,  G01N33/48 ,  G01N33/483 ,  G01N33/50 ,  G02B7/28 ,  G02B21/00 ,  G02B27/00 ,  G02B27/14 ,  G01N33/543 ,  C07H21/04 ,  G01J3/28 ,  G01N21/00 ,  G01N33/53

CPC classification number: G02B27/148 ,  B01J2219/00274 ,  C07B2200/11 ,  C12Q1/6816 ,  C12Q1/6869 ,  C12Q2600/156 ,  C40B20/04 ,  C40B60/00 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6456 ,  G01N2015/0294 ,  G01N2015/1018 ,  G01N2015/1472 ,  G01N2015/1479 ,  G01N2021/058 ,  G01N2021/6421 ,  G02B7/28 ,  G02B21/00 ,  G02B27/0012 ,  G02B27/1013 ,  G02B27/126 ,  G02B27/144 ,  G02B27/145 ,  C12Q2565/626 ,  C12Q2563/149 ,  C12Q2563/107

Abstract: Combinatorially-synthesized deoxyribonucleic acid (DNA) oligonucleotides attached to encoded beads that are hybridized to amplified and labeled genomic DNA or ribonucleic acid (RNA) are analyzed using a flow imaging system. Oligonucleotides and corresponding reporters are bound to the surfaces of a plurality of small beads such that different beads bear different oligo sequences. Each bead bears a unique optical signature comprising a predefined number of unique reporters, where each reporter comprises a predefined combination of different fluorochromes. The composite spectral signature in turn identifies the unique nucleotide sequence of its attached oligo chains. This optical signature is rapidly decoded using an imaging system to discriminate the different reporters attached to each bead in a flow in regard to color and spatial position on the bead (see fig. 15).

Abstract translation: 使用流动成像系统分析与编码的珠偶联的与扩增和标记的基因组DNA或核糖核酸（RNA）杂交的组合合成的脱氧核糖核酸（DNA）寡核苷酸。 寡核苷酸和相应的报道结合到多个小珠的表面，使得不同的珠具有不同的寡核苷酸序列。 每个珠具有独特的光学特征，其包含预定数量的唯一记录器，其中每个报告者包括不同荧光染料的预定组合。 复合光谱特征依次识别其连接的寡链的唯一核苷酸序列。 使用成像系统快速解码该光学签名，以鉴别与珠上的颜色和空间位置相关的流中附着于每个珠的不同报告者（参见图15）。

公开(公告)号：AU1189802A

公开(公告)日：2002-04-22

申请号：AU1189802

申请日：2001-10-12

Applicant: AMNIS CORP

Inventor： FROST KEITH ,  BASIJI DAVID ,  BAUER RICHARD ,  FINCH ROSALYNDE ,  ORTYN WILLIAM ,  PERRY DAVID

IPC: G01N21/64 ,  C07B61/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N15/02 ,  G01N15/10 ,  G01N15/14 ,  G01N21/05 ,  G01N33/48 ,  G01N33/483 ,  G01N33/50 ,  G02B7/28 ,  G02B21/00 ,  G02B27/00 ,  G02B27/14

公开(公告)号：EP1330650A4

公开(公告)日：2008-03-19

申请号：EP01979992

申请日：2001-10-12

Applicant: AMNIS CORP

Inventor： FROST KEITH ,  BASIJI DAVID ,  BAUER RICHARD ,  FINCH ROSALYNDE ,  ORTYN WILLIAM ,  PERRY DAVID

IPC: G01N21/64 ,  G01N33/543 ,  B01J19/00 ,  C07B61/00 ,  C07H21/04 ,  C12N15/09 ,  C12Q1/68 ,  G01J3/28 ,  G01N15/02 ,  G01N15/10 ,  G01N15/14 ,  G01N21/05 ,  G01N33/48 ,  G01N33/483 ,  G01N33/50 ,  G01N33/53 ,  G02B7/28 ,  G02B21/00 ,  G02B27/00 ,  G02B27/14

CPC classification number: G02B27/148 ,  B01J2219/00274 ,  C07B2200/11 ,  C12Q1/6816 ,  C12Q1/6869 ,  C12Q2600/156 ,  C40B20/04 ,  C40B60/00 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6456 ,  G01N2015/0294 ,  G01N2015/1018 ,  G01N2015/1472 ,  G01N2015/1479 ,  G01N2021/058 ,  G01N2021/6421 ,  G02B7/28 ,  G02B21/00 ,  G02B27/0012 ,  G02B27/1013 ,  G02B27/126 ,  G02B27/144 ,  G02B27/145 ,  C12Q2565/626 ,  C12Q2563/149 ,  C12Q2563/107

Abstract: Combinatorially-synthesized deoxyribonucleic acid (DNA) oligonucleotides attached to encoded beads that are hybridized to amplified and labeled genomic DNA or ribonucleic acid (RNA) are analyzed using a flow imaging system. Oligonucleotides and corresponding reporters are bound to the surfaces of a plurality of small beads such that different beads bear different oligo sequences. Each bead bears a unique optical signature comprising a predefined number of unique reporters, where each reporter comprises a predefined combination of different fluorochromes. The composite spectral signature in turn identifies the unique nucleotide sequence of its attached oligo chains. This optical signature is rapidly decoded using an imaging system to discriminate the different reporters attached to each bead in a flow in regard to color and spatial position on the bead (see fig. 15).