公开(公告)号：DE3177004D1

公开(公告)日：1989-04-20

申请号：DE3177004

申请日：1981-10-02

Applicant: BAXTER INT

Inventor： CULLIS HERBERT M ,  GUTIERREZ LUIS F

IPC: B04B5/02 ,  A61M1/02 ,  B04B5/04 ,  B04B9/12

Abstract: The method for separating whole blood into the components thereof is practiced with and within a separation chamber mounted in a centrifuge device during centrifugation of the chamber, the chamber having inner and outer wall surfaces and first and second side edges. The method comprises the steps of: arranging and configuring the chamber such that it has (a) an inlet on the first side thereof through which whole blood is received, (b) a first upper outlet at the top of the chamber from which plasma with particles therein is withdrawn, (c) a second lower outlet at the bottom corner of the chamber on the second side thereof from which red blood cells are withdrawn, and (d) the inner wall surface positioned in a plane including a tangent to a circle about the axis of rotation and the plane positioned about normal (in a vertical direction) to a radius extending from the axis of rotation of the centrifuge device. The method and chamber direct whole blood into the chamber from the first side thereof at a point between the bottom and top of the chamber. Heavier particles such as red blood cells are directed downwardly and outwardly along the outer wall surface toward the lower bottom corner of the chamber. At the same time, plasma is directed upwardly along the inner wall surface of the chamber, so that there is separation of white blood cells, particularly granulocytes, from the whole blood, which are directed with the plasma, toward and out the first outlet from the chamber.

公开(公告)号：AU7430487A

公开(公告)日：1987-12-01

申请号：AU7430487

申请日：1987-05-07

Applicant: BAXTER INT

Inventor： PATTILLO MARCHA C ,  BACEHOWSKI DAVID C ,  BILSTAD ARNOLD C ,  CULLIS HERBERT M ,  DENNEHEY T MICHAEL ,  YANG JAMES W ,  BROWN WILLIAM C

IPC: C12M3/00 ,  C12M1/00 ,  C12M1/26 ,  C12N5/00 ,  C12N5/10 ,  C12M1/16 ,  C12N5/02 ,  A61B19/00

Abstract: A system for metering nutrient media to cell culture containers includes a media flow conduit which defines at one end means for substantially aseptic communication with the source of the media. In a preferred embodiment, sterile connectors may be used for essentially sterile conditions. A branch conduit extends from the flow conduit at a location spaced from the ends. The branch conduit communicates with means for receiving a predetermined-volume aliquot of media through the media flow conduit from said one end. The flow conduit also defines a portion downstream from the branch conduit, which communicates with at least one cell culture container, each container being a flexible bag. At least part of the flexible bag is made of a material capable of providing at least 2 times the oxygen diffusion and at least 2 times the carbon dioxide diffusion between the bag interior and exterior, compared with an identically-sized, sealed bag having polyethylene walls 0.013 inch thick.

公开(公告)号：CA1270467A

公开(公告)日：1990-06-19

申请号：CA536719

申请日：1987-05-08

Applicant: BAXTER INT

Inventor： PATTILLO MARTHA C ,  BACEHOWSKI DAVID V ,  BILSTAD ARNOLD C ,  CULLIS HERBERT M ,  DENNEHEY T MICHAEL ,  YANG JAMES W ,  BROWN WILLIAM C

IPC: C12M3/00 ,  C12M1/00 ,  C12M1/26 ,  C12N5/00 ,  C12N5/10

Abstract: SYSTEM FOR METERING NUTRIENT MEDIA TO CELL CULTURE CONTAINERS AND METHOD A system for metering nutrient media to cell culture containers includes a media flow conduit which defines at one end means for substantially aseptic communication with the source of the media. In a preferred embodiment, sterile connectors may be used from essentially sterile conditions. A branch conduit extends from the flow conduit at a location spaced from the ends. The branch conduit communicates with means for receiving a predetermined-volume aliquot of media through the media flow conduit from said one end. The flow conduit also defines a portion downstream from the branch conduit, which communicates with at least one cell culture container, each container being a flexible bag. At least part of the flexible bag is made of a material capable of providing at least 2 times the oxygen diffusion and at least 2 times the carbon dioxide diffusion between the bag interior and exterior, compared with an identically-sized, scaled bag having polyethylene walls 0.013 inch thick.

公开(公告)号：DE2858828C2

公开(公告)日：1993-12-02

申请号：DE2858828

申请日：1978-08-11

Applicant: BAXTER INT

Inventor： BACEHOWSKI DAVID V ,  CULLIS HERBERT M ,  VAN BAELEN ARMAND R

IPC: B04B1/00 ,  B04B5/04 ,  C07K16/06 ,  C07K16/30 ,  F04B9/02 ,  F04B43/10 ,  B04B7/12 ,  B04B11/00

Abstract: A blood component separating device comprises a centrifuge bowl adapted for rotation about its axis in a centrifuge. The bowl defines an outer arcuate wall plus an inner circumferential wall, spaced from the outer wall, to define a arcuate slot adapted for receiving blood components. Access tubing is adapted for communication with the slot. Preferably, an elongated, flexible, collapsible inner liner is provided in the slot. Individual access tubing also may communicate with an elongated container interior between the ends of the elongated, collapsible container. Furthermore, a portion of the slot may define the shape of an outwardly extending spiral, to provide an increasing gravitational field upon fluids therein as the fluid moves along the slot.

公开(公告)号：DE2845364C3

公开(公告)日：1994-04-28

申请号：DE2845364

申请日：1978-10-18

Applicant: BAXTER INT

Inventor： CULLIS HERBERT M ,  DE VRIES JAMES H

IPC: B04B5/00 ,  A61M1/02 ,  A61M1/36 ,  B04B5/04 ,  A61M1/38

Abstract: An intervivos blood processing system for centrifugally separating red blood cell and platelet components from whole blood includes a separation chamber for separating red blood cell and white blood cell components, and a collection chamber for collecting platelet components. Each chamber is formed with closely-spaced sidewalls defining respective generally flat interior chambers therebetween and is mounted on a rotatably driven carriage substantially tilted from a parallel arrangement with respect to the carriage axis of rotation at a slight angle whereby the red blood cell component is caused to collect in shoulder regions of the separation chamber and the platelet component is caused to collect on the sidewalls of the collection chamber. The processing chambers are preferably each formed from sheets of flexible hemo-compatible plastic sealed about their periphery to define the chambers therebetween, and are received on the carriage between removable carrier plates having recesses which define the shape of the chambers under fluid pressure.

公开(公告)号：AU607915B2

公开(公告)日：1991-03-21

申请号：AU7430487

申请日：1987-05-07

Applicant: BAXTER INT

Inventor： PATTILLO MARCHA C ,  BACEHOWSKI DAVID C ,  BILSTAD ARNOLD C ,  CULLIS HERBERT M ,  DENNEHEY T MICHAEL ,  YANG JAMES W ,  BROWN WILLIAM C

IPC: C12M3/00 ,  C12M1/00 ,  C12M1/26 ,  C12N5/00 ,  C12N5/10 ,  C12M1/16 ,  C12N5/02 ,  A61B19/00 ,  C12M1/04

Abstract: A system for metering nutrient media to cell culture containers includes a media flow conduit which defines at one end means for substantially aseptic communication with the source of the media. In a preferred embodiment, sterile connectors may be used for essentially sterile conditions. A branch conduit extends from the flow conduit at a location spaced from the ends. The branch conduit communicates with means for receiving a predetermined-volume aliquot of media through the media flow conduit from said one end. The flow conduit also defines a portion downstream from the branch conduit, which communicates with at least one cell culture container, each container being a flexible bag. At least part of the flexible bag is made of a material capable of providing at least 2 times the oxygen diffusion and at least 2 times the carbon dioxide diffusion between the bag interior and exterior, compared with an identically-sized, sealed bag having polyethylene walls 0.013 inch thick.