公开(公告)号：AU607915B2

公开(公告)日：1991-03-21

申请号：AU7430487

申请日：1987-05-07

Applicant: BAXTER INT

Inventor： PATTILLO MARCHA C ,  BACEHOWSKI DAVID C ,  BILSTAD ARNOLD C ,  CULLIS HERBERT M ,  DENNEHEY T MICHAEL ,  YANG JAMES W ,  BROWN WILLIAM C

IPC: C12M3/00 ,  C12M1/00 ,  C12M1/26 ,  C12N5/00 ,  C12N5/10 ,  C12M1/16 ,  C12N5/02 ,  A61B19/00 ,  C12M1/04

Abstract: A system for metering nutrient media to cell culture containers includes a media flow conduit which defines at one end means for substantially aseptic communication with the source of the media. In a preferred embodiment, sterile connectors may be used for essentially sterile conditions. A branch conduit extends from the flow conduit at a location spaced from the ends. The branch conduit communicates with means for receiving a predetermined-volume aliquot of media through the media flow conduit from said one end. The flow conduit also defines a portion downstream from the branch conduit, which communicates with at least one cell culture container, each container being a flexible bag. At least part of the flexible bag is made of a material capable of providing at least 2 times the oxygen diffusion and at least 2 times the carbon dioxide diffusion between the bag interior and exterior, compared with an identically-sized, sealed bag having polyethylene walls 0.013 inch thick.

公开(公告)号：AU7430487A

公开(公告)日：1987-12-01

申请号：AU7430487

申请日：1987-05-07

Applicant: BAXTER INT

Inventor： PATTILLO MARCHA C ,  BACEHOWSKI DAVID C ,  BILSTAD ARNOLD C ,  CULLIS HERBERT M ,  DENNEHEY T MICHAEL ,  YANG JAMES W ,  BROWN WILLIAM C

IPC: C12M3/00 ,  C12M1/00 ,  C12M1/26 ,  C12N5/00 ,  C12N5/10 ,  C12M1/16 ,  C12N5/02 ,  A61B19/00

Abstract: A system for metering nutrient media to cell culture containers includes a media flow conduit which defines at one end means for substantially aseptic communication with the source of the media. In a preferred embodiment, sterile connectors may be used for essentially sterile conditions. A branch conduit extends from the flow conduit at a location spaced from the ends. The branch conduit communicates with means for receiving a predetermined-volume aliquot of media through the media flow conduit from said one end. The flow conduit also defines a portion downstream from the branch conduit, which communicates with at least one cell culture container, each container being a flexible bag. At least part of the flexible bag is made of a material capable of providing at least 2 times the oxygen diffusion and at least 2 times the carbon dioxide diffusion between the bag interior and exterior, compared with an identically-sized, sealed bag having polyethylene walls 0.013 inch thick.