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1.发明申请CONTINUOUS CENTRIFUGATION PROCESS FOR THE SEPARATION OF BIOLOGIC COMPONENTS FROM HETEROGENEOUS CELL POPULATIONS 审中-公开用于分离异源细胞群体生物成分的连续离心方法
公开(公告)号：WO9427698A3
公开(公告)日：1996-10-10
申请号：PCT/US9305735
申请日：1993-06-15
Applicant: BAXTER INT
Inventor： LAKE WILLIAM C , GIESLER RICHARD , VAN EPPS DENNIS , CHAPMAN JOHN R , MARTINSON JEFFREY A , ELLIS DALE R , AONO FREDERICK , BISCHOF DANIEL F
IPC: G01N33/48 , A61K35/14 , A61K35/24 , A61M1/36 , B03B5/28 , C12M1/00 , C12N5/00 , C12N5/06 , C12Q1/02 , C12Q1/24 , G01N33/49 , G01N33/50 , G01N33/53 , G01N33/569 , B01D
CPC classification number: G01N33/56972 , A61M1/3618 , A61M1/362 , A61M1/3696 , C12Q1/24 , G01N33/5005 , G01N33/569
Abstract: A method combining the techniques of immunoaffinity separation and continuous flow centrifugal separation is provided for selective separation of a nucleated heterogeneous cell population from a heterogeneous cell mixture. The heterogeneous cell mixture is intimately contacted to promote binding thereto by particles having attached a substance that actively binds to a specific desired type of cell out of the cell mixture. The particles are selected so that the sedimentation velocity of the particle/cell conjugate differs sufficiently from those of other cells in the cell mixture to allow its separation by means of a continuous flow cell separator. The method rapidly processes large volumes of cell mixture with the high accuracy expected of immunoaffinity separation and can be used to separate, for example, various types of leukocytes from whole blood, bone marrow concentrate, or a peripheral blood stem cell concentrate; or precursors of lymphokine activated killer cells, tumor infiltrating lymphocyte cells, or activated killer monocytes from lymphocyte or monocyte cell concentrates or from a tissue cell preparation.
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Patent Agency Ranking2.发明公开
公开(公告)号：EP0653062A4
公开(公告)日：1997-12-03
申请号：EP93915369
申请日：1993-06-15
Applicant: BAXTER INT
Inventor： LAKE WILLIAM C , GIESLER RICHARD , VAN EPPS DENNIS , CHAPMAN JOHN R , MARTINSON JEFFREY A , ELLIS DALE R , AONO FREDERICK , BISCHOF DANIEL F
IPC: G01N33/48 , A61K35/14 , A61K35/24 , A61M1/36 , B03B5/28 , C12M1/00 , C12N5/00 , C12N5/06 , C12Q1/02 , C12Q1/24 , G01N33/49 , G01N33/50 , G01N33/53 , G01N33/569
CPC classification number: G01N33/56972 , A61M1/3618 , A61M1/362 , A61M1/3696 , C12Q1/24 , G01N33/5005 , G01N33/569
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公开(公告)号：AT382633T
公开(公告)日：2008-01-15
申请号：AT01941591
申请日：2001-05-23
Applicant: BAXTER HEALTHCARE SA , BAXTER INT
Inventor： SCHAEFFER ANDREW , TSENG-LAW JANET , THORNTON JEFFREY , VAN EPPS DENNIS
IPC: C07K7/06 , G01N33/53 , A61K35/12 , A61K47/48 , A61P35/00 , A61P37/04 , A61P37/06 , C07K1/04 , C07K7/08 , C07K16/28 , C07K16/30 , C07K19/00 , C12N5/06 , C12N5/08 , C12Q1/02 , G01N33/543 , G01N33/569 , G01N33/574
Abstract: The present invention provides peptide-ligand conjugates containing a peptide, where a free form of the peptide is capable of displacing a second ligand specific for the peptide. The invention also provides a method of selecting a cell. The method includes the steps of contacting a cell population with a peptide-ligand conjugate, wherein the conjugate comprises a first ligand specific for a cell in the population conjugated to a peptide capable of displacing a second ligand specific for the peptide, thereby forming a cell-conjugate complex; contacting the peptide-ligand conjugate with the second ligand; and isolating the cell-conjugate complex. The method can further include the step of adding a free form of the peptide to release the cell from the complex. The invention also provides a subpopulation of cells containing two or more specifically isolated cells and methods of isolating cell subpopulations. The invention additionally provides a method of diagnosing a condition using peptide-ligand conjugates. The invention further provides a kit for isolating a cell containing a peptide capable of displacing an antibody specific for the peptide and the antibody.
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公开(公告)号：DE69332926T2
公开(公告)日：2004-03-11
申请号：DE69332926
申请日：1993-06-15
Applicant: BAXTER INT
Inventor： LAKE C , GIESLER RICHARD , VAN EPPS DENNIS , CHAPMAN R , MARTINSON A , ELLIS R , AONO FREDERICK , BISCHOF F
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公开(公告)号：DE69332926D1
公开(公告)日：2003-06-05
申请号：DE69332926
申请日：1993-06-15
Applicant: BAXTER INT
Inventor： LAKE C , GIESLER RICHARD , VAN EPPS DENNIS , CHAPMAN R , MARTINSON A , ELLIS R , AONO FREDERICK , BISCHOF F
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公开(公告)号：CA2140455A1
公开(公告)日：1994-12-08
申请号：CA2140455
申请日：1993-06-15
Applicant: BAXTER INT
Inventor： LAKE WILLIAM C , GIESLER RICHARD , VAN EPPS DENNIS , CHAPMAN JOHN R , MARTINSON JEFFREY A , ELLIS DALE R , AONO FREDERICK , BISCHOF DANIEL F
IPC: G01N33/48 , A61K35/14 , A61K35/24 , A61M1/36 , B03B5/28 , C12M1/00 , C12N5/00 , C12N5/06 , C12Q1/02 , C12Q1/24 , G01N33/49 , G01N33/50 , G01N33/53 , G01N33/569 , A61K35/18 , A61K35/28 , B01D21/26 , C12N1/00
Abstract: A method combining the techniques of immunoaffinity separation and continuous flow centrifugal is provided for selective separation of a nucleated heterogeneous cell population from a heterogeneous cell mixture. The heterogeneous cell mixture is intimately contacted to promote binding thereto by particles having attached a substance that actively binds to a specific desired type of cell out of the cell mixture. The particles are selected so that the sedimentation velocity of the particle/cell conjugate differs sufficiently from those of other cells in the cell mixture to allow its separation by means of a continuous flow cell separator. The method rapidly processes large volumes of cell mixture with the high accuracy expected of immunoaffinity separation and can be used to separate, for example, various types of leukocytes from whole blood, bone marrow concentrate, or a peripheral blood stem cell concentrate; or precursors of lymphokine activated killer cells, tumor infiltrating cells, or activated killer monocytes from lymphocyte or monocyte cell concentrates or from a tissue cell preparation.
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