公开(公告)号：GB2485850A

公开(公告)日：2012-05-30

申请号：GB201100787

申请日：2010-11-25

Applicant: BIO RAD LABORATORIES

Inventor： HINDSON BENJAMIN JOSEPH ,  SAXONOV SERGE ,  BELGRADER PHILIP ,  NESS KEVIN DEAN ,  LUCERO MICHAEL ,  COLSTON JR BILLY WAYNE

IPC: C12Q1/68

Abstract: Methods to determine nucleic acid copy numbers within a genetic material, such as chromosome number for the detection of foetal genetic or chromosomal diseases such as trisomy or tetraploidy in Downâ s syndrome, trisomy 8, Kleinfelterâ s syndrome, Patau syndrome, Edwards syndrome or XXX syndrome are disclosed wherein a first ligation probe (half-probe) specific for a first target sequence is hybridised to a target nucleic acid, a second ligation probe (half-probe) specific for a second target sequence is hybridised to an adjacent nucleic acid sequence and the two half-probes are joined by ligation prior to partitioning, preferably in an water-in-oil emulsion and subjected to quantitative amplification, preferably by a quantitative PCR approach. A microcapsule comprising a solid-phase exterior and an aqueous phase containing a ligated probe and a water-in-oil mixture comprising a ligation probes are also disclosed.

公开(公告)号：AU2009297108B2

公开(公告)日：2015-02-12

申请号：AU2009297108

申请日：2009-09-23

Applicant: BIO RAD LABORATORIES

Inventor： COLSTON JR BILLY WAYNE ,  HINDSON BENJAMIN JOSEPH ,  NESS KEVIN DEAN ,  MASQUELIER DONALD ARTHUR ,  MILANOVICH FRED PAUL ,  MODLIN DOUGLAS N ,  RIOT VINCENT ,  BURD SAMUEL ,  MAKAREWICZ JR ANTHONY JOSEPH ,  BELGRADER PHILLIP

IPC: G01N21/00

Abstract: Systems, including apparatus and methods, for performing assays. These systems may involve separating sample components by partitioning them into droplets or other partitions, amplifying or otherwise reacting the components within the droplets, detecting the amplified components, or characteristics thereof, and/or analyzing the resulting data, among others.