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公开(公告)号：CA2721582C
公开(公告)日：2017-07-11
申请号：CA2721582
申请日：2009-04-17
Applicant: BIO-RAD LABORATORIES INC
Inventor： WATKINS MICHAEL I , MARR GREGORY A , YANG XIAOYUN , BRUEHL RICHARD , SHAN DAMING , COLEMAN PATRICK F
IPC: C12N15/29 , A61K38/00 , C07K14/415 , C07K19/00 , C12N15/63 , G01N33/53 , G01N33/564 , G01N33/68
Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.
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公开(公告)号：AU2009239558B2
公开(公告)日：2013-05-02
申请号：AU2009239558
申请日：2009-04-17
Applicant: BIO RAD LABORATORIES
Inventor： BRUEHL RICHARD , MARR GREGORY A , SHAN DAMING , WATKINS MICHAEL I , YANG XIAOYUN , COLEMAN PATRICK F
Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.
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Patent Agency Ranking
公开(公告)号：AU2009239558A1
公开(公告)日：2009-10-29
申请号：AU2009239558
申请日：2009-04-17
Applicant: BIO RAD LABORATORIES
Inventor： WATKINS MICHAEL I , BRUEHL RICHARD , YANG XIAOYUN , SHAN DAMING , COLEMAN PATRICK F , MARR GREGORY A
Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.
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公开(公告)号：AU2013208412A1
公开(公告)日：2013-08-22
申请号：AU2013208412
申请日：2013-07-22
Applicant: BIO RAD LABORATORIES
Inventor： WATKINS MICHAEL I , MARR GREGORY A , YANG XIAOYUN , BRUEHL RICHARD , SHAN DAMING , COLEMAN PATRICK F
Abstract: Abstract The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase. Fig. 1
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公开(公告)号：CA2721582A1
公开(公告)日：2009-10-29
申请号：CA2721582
申请日：2009-04-17
Applicant: BIO RAD LABORATORIES
Inventor： WATKINS MICHAEL I , MARR GREGORY A , YANG XIAOYUN , BRUEHL RICHARD , SHAN DAMING , COLEMAN PATRICK F
Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.
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公开(公告)号：WO2009131909A2
公开(公告)日：2009-10-29
申请号：PCT/US2009040943
申请日：2009-04-17
Applicant: BIO RAD LABORATORIES , WATKINS MICHAEL I , MARR GREGORY A , YANG XIAOYUN , BRUEHL RICHARD , SHAN DAMING , COLEMAN PATRICK F
Inventor： WATKINS MICHAEL I , MARR GREGORY A , YANG XIAOYUN , BRUEHL RICHARD , SHAN DAMING , COLEMAN PATRICK F
IPC: G01N33/53 , C07H21/04 , C07K14/415 , C12N5/10 , C12N15/63
CPC classification number: G01N33/564 , C07K14/415 , C07K2319/20 , C07K2319/23 , C07K2319/70 , C12N9/1044 , G01N2333/415 , G01N2333/91085 , G01N2800/24
Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.
Abstract translation: 本发明提供一种用于通过使来自受试者的体液样品与固定在固体支持物上的麦醇溶蛋白融合蛋白形成的抗原来确定受试者是否患有乳糜泻的方法。 抗原的麦醇溶蛋白融合蛋白包括与诸如谷胱甘肽-S转移酶（GST）蛋白质的标签连接的重组脱酰胺麦醇溶蛋白。 通过经由标签固定在固体支持物上的麦醇溶蛋白融合蛋白来制备抗原。 抗原可以进一步包括与麦醇溶蛋白融合蛋白交联的组织转谷氨酰胺酶（tTG）。 当存在tTG时，将tTG和重组脱酰基麦醇溶蛋白在固定固相之前混合在一起。
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公开(公告)号：EP2276499A4
公开(公告)日：2012-02-08
申请号：EP09734340
申请日：2009-04-17
Applicant: BIO RAD LABORATORIES
Inventor： WATKINS MICHAEL I , MARR GREGORY A , YANG XIAOYUN , BRUEHL RICHARD , SHAN DAMING , COLEMAN PATRICK F
CPC classification number: G01N33/564 , C07K14/415 , C07K2319/20 , C07K2319/23 , C07K2319/70 , C12N9/1044 , G01N2333/415 , G01N2333/91085 , G01N2800/24
Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.
Abstract translation: 本发明提供了一种用于通过使来自受试者的体液样品与固定在固体支持物上的麦醇溶蛋白融合蛋白形成的抗原来确定受试者是否患有乳糜泻的方法。 抗原的麦醇溶蛋白融合蛋白包括与诸如谷胱甘肽-S转移酶（GST）蛋白质的标签连接的重组脱酰胺麦醇溶蛋白。 通过经由标签固定在固体支持物上的麦醇溶蛋白融合蛋白来制备抗原。 抗原可以进一步包括与麦醇溶蛋白融合蛋白交联的组织转谷氨酰胺酶（tTG）。 当存在tTG时，将tTG和重组脱酰基麦醇溶蛋白在固定固相之前混合在一起。
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