Abstract:
Un método para determinar la presencia de anticuerpos de VIH en un fluido corporal, incluyendo: (a) contacto en condiciones que permitan la unión inmunoespecífica para formar una mezcla de reacción del fluido corporal con una composición que contenga por lo menos un polipéptido seleccionado del grupo consistente en las secuencias de aminoácidos: **(Ver fórmula)** donde W es un H del grupo amino terminal NH2 del polipéptido, o uno o más aminoácidos adicionales ligados al grupo amino terminal NH2 del polipéptido, seleccionándose los aminoácidos adicionales para facilitar el acoplamiento del polipéptido a una proteína portadora o a un soporte; X está ausente, Cys-Gly-Gly; o Lys-Lys, Y está ausente, Cys o uno o más aminoácidos añadidos para facilitar el acoplamiento; y Z es OH o NH; (b) detectar si se ha producido unión inmunoespecífica entre el polipéptido y un componente anticuerpo del fluido corporal formando un complejo inmune e indicando la detección del complejo inmune la presencia de anticuerpos de VIH en el fluido corporal.
Abstract:
The present invention relates to peptides that are derived from HSV-2 glycoprotein G2 and represent HSV-2 type-specific epitopes. The present invention also provides for compositions comprising these peptides for type-specific serological diagnosis of HSV-2 infection. Methods of using these peptides for type-specific detection of HSV-2 antibodies and differentiation of HSV-2 viral infection from HSV-1 and other herpes family viral infections are further provided.
Abstract:
The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.
Abstract:
The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.
Abstract:
Un método para determinar la presencia de anticuerpos para el virus de la inmunodeficiencia humana (HIV) en un fluido corporal, que comprende: (a) poner en contacto, en condiciones que permitan la unión inmunoespecífica para formar una mezcla de reacción, el fluido corporal con una composición que contiene al menos un polipéptido o proteína que consisten las siguientes secuencias de aminoácidos: (III) BRU 124F1X W-X-Lys-Ile-Gln-Asn-Phe-Arg-Val-Tyr-Tyr-Arg-Asp-Ser- Arg-Asp-Pro-Leu-Trp-Lys-GIy-Pro-Ala-Lys-Leu-Leu-Trp- Lys-Gly-Glu-Gly-Ala-Val-Val-Ile-GIn-Asp-Asn-Ser-Asp- Ile-Lys-Y-Z (IV)BR 124F3X W-X-Lys-Ile-Gln-Asn-Phe-Arg-Val-Tyr-Tyr-Arg-Asp-Ser- Arg-Asp-Pro-Leu-Trp-Lys-Gly-Pro-Ala-Lys-Leu-Leu-Trp- Lys-Gly-Glu-Gly-Ala-Val-Val-Ile-Gln-Asp-Asn-Y-Z (XIII) pol7-aa W-S-Tyr-Ser-Ala-Gly-Glu-Arg-Ile-Val-Asp-Ile-Ile-Ala-Thr- Asp-Ile-Gln-Thr-Lys-Glu-Leu-GIn-Lys-Gln-Ile-Thr-Lys-Ile- Gln-Asn-Phe-Arg-Val-Tyr-Tyr-Arg-Asp-Ser-Arg-Asp-Pro- Leu-Trp-Lys-Gly-Pro-Ala-Lys-Leu-Leu-Trp-Lys-Gly-Glu- Gly-Ala-Val-Val-lle-Gln-Asp-Asn-Ser-Asp-lle-Lys-Val-Val- Pro-Arg-Arg-Lys-Ala-Lys-Ile-Ile-Arg-Asp-Tyr-Gly-Lys-Gln- Met-Ala-GIy-Asp-Asp-Y-Z
Abstract:
Novel polypeptides, and recombinant polynucleotide sequences encoding the same, are provided. The polypeptides have substantially the same sequence as immunologically significant fragments of AIDS-related viruses. The polypeptides can be used as reagents in the determination of exposure of a human host to the virus. Of particular interest is the use of polypeptides in screening blood products.
Abstract:
Abstract The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase. Fig. 1
Abstract:
The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.